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The map label for this gene is kipI [H]

Identifier: 92113158

GI number: 92113158

Start: 1160762

End: 1161493

Strand: Direct

Name: kipI [H]

Synonym: Csal_1030

Alternate gene names: 92113158

Gene position: 1160762-1161493 (Clockwise)

Preceding gene: 92113157

Following gene: 92113159

Centisome position: 31.4

GC content: 67.9

Gene sequence:

>732_bases
GTGAACGACCCGAAAATCGAAACGGTGGGCATGGACACGCTCATCGTGCGCCTGTTCGACGGCATCGATGAAGCTCACAT
GCCATGGATGCTGGCCGCCGATCGCGCTCTGCGCGAGGCCTTTGGCACGGCATTGATCGACCTGGTGCCGTCCTACACCA
CCTTGCTGGTGCACTACGACGTGGCGCACCTGACGCTGCCGGCGGCCCGTGCCCGGGTGCGCGAGGCGCTCGACGGCTTG
TCGCCGACGGCCGAGCGTGAAGGGCGGCGGCATGTGATCCCGGTGTGGTATGACGTGAGTGTCGGGCCGGAACTCGAGCG
CATCGCCGCCCGCCTGGGCGTGAGTCATGACCAAGTCGTCGCACGGCACTGCGCGCGCGATTACAGCGTCTTCGCGCTGG
GCTTCGCACCGGGGTACGCCTTCATGGGGCGTCTCGACGAAGAGCTGGCCACGCCACGCCTGAAGACGCCCCGCCAGAAA
GTCGCCGCCGGCAGCGTGGCCATCGCCGAACGCCAGACGGCGGTCTATCCCACGCTCTCGCCCGGCGGCTGGAATATCGT
CGGGCGCACGCCCGTGGCCCTGTTCGGAGACGACCGGGAAGGCTACAGCCTGTTCCGGCCGGGCGATAAAGTGCGCTTCG
AGGCGATTTCCCGCGAGGCCTTCATCGAGCAGGGCGGCGACCCGACACCGCTGGCAGAGCGCGGCGATACACCGACGGAG
GAACAGGCATGA

Upstream 100 bases:

>100_bases
CCGGCGGATACCTTGTGCGTGCATGGCGACAACGCCGAATCGGTAGCTGCCGTACGCGCCATTCGCGATGCCTTCGATGG
CCTGGCGCGGGAGTGAGCCG

Downstream 100 bases:

>100_bases
AAGGGTTGGTCGTCGAACGCGCGGGACCGTTGGCGCTGATTCAGGATGGCGGGCGCTTCGGCGTGCGTCACCTTGGCGTG
ACCCAGGGAGGCGCCGCCGA

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNDPKIETVGMDTLIVRLFDGIDEAHMPWMLAADRALREAFGTALIDLVPSYTTLLVHYDVAHLTLPAARARVREALDGL
SPTAEREGRRHVIPVWYDVSVGPELERIAARLGVSHDQVVARHCARDYSVFALGFAPGYAFMGRLDEELATPRLKTPRQK
VAAGSVAIAERQTAVYPTLSPGGWNIVGRTPVALFGDDREGYSLFRPGDKVRFEAISREAFIEQGGDPTPLAERGDTPTE
EQA

Sequences:

>Translated_243_residues
MNDPKIETVGMDTLIVRLFDGIDEAHMPWMLAADRALREAFGTALIDLVPSYTTLLVHYDVAHLTLPAARARVREALDGL
SPTAEREGRRHVIPVWYDVSVGPELERIAARLGVSHDQVVARHCARDYSVFALGFAPGYAFMGRLDEELATPRLKTPRQK
VAAGSVAIAERQTAVYPTLSPGGWNIVGRTPVALFGDDREGYSLFRPGDKVRFEAISREAFIEQGGDPTPLAERGDTPTE
EQA
>Mature_243_residues
MNDPKIETVGMDTLIVRLFDGIDEAHMPWMLAADRALREAFGTALIDLVPSYTTLLVHYDVAHLTLPAARARVREALDGL
SPTAEREGRRHVIPVWYDVSVGPELERIAARLGVSHDQVVARHCARDYSVFALGFAPGYAFMGRLDEELATPRLKTPRQK
VAAGSVAIAERQTAVYPTLSPGGWNIVGRTPVALFGDDREGYSLFRPGDKVRFEAISREAFIEQGGDPTPLAERGDTPTE
EQA

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=133, Percent_Identity=47.3684210526316, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26709; Mature: 26709

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPKIETVGMDTLIVRLFDGIDEAHMPWMLAADRALREAFGTALIDLVPSYTTLLVHYD
CCCCCEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
VAHLTLPAARARVREALDGLSPTAEREGRRHVIPVWYDVSVGPELERIAARLGVSHDQVV
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHH
ARHCARDYSVFALGFAPGYAFMGRLDEELATPRLKTPRQKVAAGSVAIAERQTAVYPTLS
HHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCEEECCCCC
PGGWNIVGRTPVALFGDDREGYSLFRPGDKVRFEAISREAFIEQGGDPTPLAERGDTPTE
CCCCEEECCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EQA
CCC
>Mature Secondary Structure
MNDPKIETVGMDTLIVRLFDGIDEAHMPWMLAADRALREAFGTALIDLVPSYTTLLVHYD
CCCCCEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
VAHLTLPAARARVREALDGLSPTAEREGRRHVIPVWYDVSVGPELERIAARLGVSHDQVV
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHH
ARHCARDYSVFALGFAPGYAFMGRLDEELATPRLKTPRQKVAAGSVAIAERQTAVYPTLS
HHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCEEECCCCC
PGGWNIVGRTPVALFGDDREGYSLFRPGDKVRFEAISREAFIEQGGDPTPLAERGDTPTE
CCCCEEECCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EQA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]