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The map label for this gene is yiaO [H]

Identifier: 92112443

GI number: 92112443

Start: 348947

End: 349966

Strand: Direct

Name: yiaO [H]

Synonym: Csal_0309

Alternate gene names: 92112443

Gene position: 348947-349966 (Clockwise)

Preceding gene: 92112442

Following gene: 92112444

Centisome position: 9.44

GC content: 64.22

Gene sequence:

>1020_bases
ATGAAGCACGCCATCTCCTATTGCCTGCTGGGGCTCGGCCTCACCGCCGCCACCGGCGTTCACGCCGACACCACGTTGCG
CCTGGGCCATCTATGGCCGGCGCAGTCCGCCGTCAATCAGGACTTGATCAAGGCCTGGGCCGATCAGGTCACGCAAGACG
CGAATGGTGCGCTCGAGGTGGAAATCTATCCCTCGCAGACACTGAGCAAGGCCAGCAGCGCCTACGAGGGCGTCGTCAAC
GGCGTCGCCGATATCACCGTCACGCTGCAGGGCTATACGGCGGGACGCTTTCCGCTCACCGAGATCGTGCAGTTGCCGGG
GGTATCGGCCAGTGCACCGCAAGGCGCCTGTATCCTGCAGTCGCTTTACGATGACGGGGCGTTGGGGGACGAATACGCCG
ACTCCCATCTGCTGTTCCTGTTCACTACTGGTCCGGCACCGCTGCACACACGCGACACGGAGGTGCAAACGCCGGCGGAT
CTCGAGGGCCTGCGCATCCGCACGCCCAGCGAAGTCTCCAGCGACATGCTGGAAAGCATGGGGGCCGACCCGGTGGGCAT
GCCGGCACCGGACATCTACACCTCGCTGCAGCGTGGCGTGATCGATGGTCTGAGCCTTCCCTGGGAAGCCATGAAGGTCT
TCCAGGTCAACGAACTGGCCAGCTATCACCTGGATATTCCCTACTACACGGGCGCCTTGATGGCGACCATGAATCGCGAT
GCCTACGAGGCGCTGTCGCCGGAAGCACGCGAGGCCATCGATGCCAATACCGGCATGGGCTGGGCAGAGAAGGCGGGCGA
GGTCTTCTATCGACTCGATAGCGAAGGCAAGGCGCAGGCACGCGAGCAGGGCGACACCATCCACCACGTCGAGGACCCCC
TGAACGATCCGCAGTGGTCAGGTCCCTTGAAGCGTGGTACCGAGCGCTACCTCGAACGCCTGGAGGAACGCGGCATCGAC
AACGCACGAGCGGTATACGAGCAGGCGCTGTCCCTGCGGGATGGTGCCTGCGCCCGCTGA

Upstream 100 bases:

>100_bases
CGTGATCGAGGTGCCGACAACGACCATCGAGCCCTGAGGGCTTGGGCGTTCGACGAGCGCCATGTCAGTGAACATCCAAC
AATGATAACGAGGTATCACC

Downstream 100 bases:

>100_bases
TCGTTTGGCTGGACTGCGCCGTGGCCGGTACGGCGCGGCGGCTGGTATCCTCGATGCATCGAGGCTGACCCGGGCGAGGC
GCGAGATGACGGCGCACGCG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKHAISYCLLGLGLTAATGVHADTTLRLGHLWPAQSAVNQDLIKAWADQVTQDANGALEVEIYPSQTLSKASSAYEGVVN
GVADITVTLQGYTAGRFPLTEIVQLPGVSASAPQGACILQSLYDDGALGDEYADSHLLFLFTTGPAPLHTRDTEVQTPAD
LEGLRIRTPSEVSSDMLESMGADPVGMPAPDIYTSLQRGVIDGLSLPWEAMKVFQVNELASYHLDIPYYTGALMATMNRD
AYEALSPEAREAIDANTGMGWAEKAGEVFYRLDSEGKAQAREQGDTIHHVEDPLNDPQWSGPLKRGTERYLERLEERGID
NARAVYEQALSLRDGACAR

Sequences:

>Translated_339_residues
MKHAISYCLLGLGLTAATGVHADTTLRLGHLWPAQSAVNQDLIKAWADQVTQDANGALEVEIYPSQTLSKASSAYEGVVN
GVADITVTLQGYTAGRFPLTEIVQLPGVSASAPQGACILQSLYDDGALGDEYADSHLLFLFTTGPAPLHTRDTEVQTPAD
LEGLRIRTPSEVSSDMLESMGADPVGMPAPDIYTSLQRGVIDGLSLPWEAMKVFQVNELASYHLDIPYYTGALMATMNRD
AYEALSPEAREAIDANTGMGWAEKAGEVFYRLDSEGKAQAREQGDTIHHVEDPLNDPQWSGPLKRGTERYLERLEERGID
NARAVYEQALSLRDGACAR
>Mature_339_residues
MKHAISYCLLGLGLTAATGVHADTTLRLGHLWPAQSAVNQDLIKAWADQVTQDANGALEVEIYPSQTLSKASSAYEGVVN
GVADITVTLQGYTAGRFPLTEIVQLPGVSASAPQGACILQSLYDDGALGDEYADSHLLFLFTTGPAPLHTRDTEVQTPAD
LEGLRIRTPSEVSSDMLESMGADPVGMPAPDIYTSLQRGVIDGLSLPWEAMKVFQVNELASYHLDIPYYTGALMATMNRD
AYEALSPEAREAIDANTGMGWAEKAGEVFYRLDSEGKAQAREQGDTIHHVEDPLNDPQWSGPLKRGTERYLERLEERGID
NARAVYEQALSLRDGACAR

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=265, Percent_Identity=27.1698113207547, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36713; Mature: 36713

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAISYCLLGLGLTAATGVHADTTLRLGHLWPAQSAVNQDLIKAWADQVTQDANGALEV
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
EIYPSQTLSKASSAYEGVVNGVADITVTLQGYTAGRFPLTEIVQLPGVSASAPQGACILQ
EECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHH
SLYDDGALGDEYADSHLLFLFTTGPAPLHTRDTEVQTPADLEGLRIRTPSEVSSDMLESM
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHC
GADPVGMPAPDIYTSLQRGVIDGLSLPWEAMKVFQVNELASYHLDIPYYTGALMATMNRD
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
AYEALSPEAREAIDANTGMGWAEKAGEVFYRLDSEGKAQAREQGDTIHHVEDPLNDPQWS
HHHHCCCHHHHHHCCCCCCCHHHHHCCEEEEECCCCHHHHHHCCCCCHHHHCCCCCCCCC
GPLKRGTERYLERLEERGIDNARAVYEQALSLRDGACAR
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKHAISYCLLGLGLTAATGVHADTTLRLGHLWPAQSAVNQDLIKAWADQVTQDANGALEV
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
EIYPSQTLSKASSAYEGVVNGVADITVTLQGYTAGRFPLTEIVQLPGVSASAPQGACILQ
EECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHH
SLYDDGALGDEYADSHLLFLFTTGPAPLHTRDTEVQTPADLEGLRIRTPSEVSSDMLESM
HHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHC
GADPVGMPAPDIYTSLQRGVIDGLSLPWEAMKVFQVNELASYHLDIPYYTGALMATMNRD
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
AYEALSPEAREAIDANTGMGWAEKAGEVFYRLDSEGKAQAREQGDTIHHVEDPLNDPQWS
HHHHCCCHHHHHHCCCCCCCHHHHHCCEEEEECCCCHHHHHHCCCCCHHHHCCCCCCCCC
GPLKRGTERYLERLEERGIDNARAVYEQALSLRDGACAR
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]