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The map label for this gene is mdlC [H]

Identifier: 92112442

GI number: 92112442

Start: 347297

End: 348883

Strand: Direct

Name: mdlC [H]

Synonym: Csal_0308

Alternate gene names: 92112442

Gene position: 347297-348883 (Clockwise)

Preceding gene: 92112440

Following gene: 92112443

Centisome position: 9.39

GC content: 67.36

Gene sequence:

>1587_bases
ATGCCAACCGTTCATTCTGCGACTTATGACTTGTTGCGCCGTCAGGGAATGACGACGATTTTCGGAAATCCTGGCTCCAA
TGAGTTGCCTTTCCTCAAGAATTTTCCTGCCGATTTTCGTTATATTCTCGGTCTTCACGAGGGCGTGGTCGTCGGCATGG
CGGATGGCTTCGCATTGGCCAGCGGGGCGCCGGCGCTGGTCAACCTGCATGCCGCCGCCGGCTCCGGCAATGCCATGGGC
GCCTTGACCAACGCCTGGTATTCGCATTCGCCGCTGGTGCTGACCGCGGGACAGCAGGCCCGTTCGATGATCGGCGTGGA
ATCGATGCTGGCCAATGTCGATGCGCCGCAGCTGCCCCGCCCCCTGGTCAAGTGGAGCTATGAGCCGGCGTGTCCCGAGG
ACGTGCCACGGGCCCTGAGCCAGGCGATCCACACGGCCTGCCTGCCGGCGCGCGGTCCGGTTTACGTGTCGATTCCCCAC
GATGACTGGGGGCACGCGGTCGATGACGATGCCGCGCTGCTCAGCGCCCGTCAGGTGGCGAGCGCCGCGCAACCCGCCGA
GACCCAGGTCGAGGCGCTCGTCGAGCGCCTGAACGCGGCCCGCAACCCGGTTCTGGTGCTGGGGCCGGATGTGGACGCCT
ATGGCGCCAATGCCCTGGCGGTGACCCTGGCCGAGCGCCTGAATGCGCCGGCCTGGGTGGCGCCGTCGGCCTCGCGCTGC
CCTTTCCCGACGCGTCATGCCTGCTTTCGTGGCGTCCTGCCGGCGGCGATCCAGGGGATTTCGGACCGCCTCGACGGTCA
CGATCTCATCGTCGTCATCGGTGCGCCGGTCTTCCGGTATCACCAGTACGCGCCGGGGCGTTACCTGCCCGATGGCGCCG
AACTGGTTCATCTGACCTGCGACATGCAGGAGGCCGCACGGGCACCCATGGGCGATGCCCTGGTGGGTGACATCCACGCC
ATGCTGGACCTGCTGGTGCCGCGTGTCCGGCAAAGCGGTCGGGCGCGTCCCGAGCCGCAAGCCGCCCCCGAGCCGCAGGT
CGACCCCGAGGGGATGCTGACACCGGAGACGGTGTTCGACGTCATGAACGCGATGGCGCCGGAGGACGCGATCTACGTCA
AGGAATCCACCTCGACGGTCACGGCGTTCTGGGAGCGAGTGGAAATGCGCCACCCGGGCAGTTACTTCTTTCCCGCCGCC
GGCGGGCTGGGCTTCGGCTTGCCGGCGGCCGTCGGCGTGCAGCTGGCCTGTCCCGAACGCCGGGTGATCGGGGTGATCGG
CGACGGCTCGGCGAATTACGCCATCACCGCGCTGTGGAGCGCTGCGCAATACCGCATTCCGGTGGTCTTCGTGATCTTGA
AGAACGGCACCTACGGTGCCCTGCGCTGGTTCTCCCGTCTGCTGGATGCGGAGGATTCGCCCGGCCTCGACGTGCCCGGT
CTCGACTTCTGTGCCCTGGCCGAGGGCTATGGCGTCGAGGCCGAGCAGGCGGCGTCCCGGGAAGCCTTCGAGGCAGCCCT
GAAAGCGGCCCTTGCCGATGATCGGCCGCGCGTGATCGAGGTGCCGACAACGACCATCGAGCCCTGA

Upstream 100 bases:

>100_bases
CATCCGAATAAGTCCCATTTATTTTGTTTAGAAAGAATAATGATTTGAGTGGTATTGGCAAGAAAACTATCGTTCAAGAA
CACGACGTTTCGAGGTGGTT

Downstream 100 bases:

>100_bases
GGGCTTGGGCGTTCGACGAGCGCCATGTCAGTGAACATCCAACAATGATAACGAGGTATCACCATGAAGCACGCCATCTC
CTATTGCCTGCTGGGGCTCG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MPTVHSATYDLLRRQGMTTIFGNPGSNELPFLKNFPADFRYILGLHEGVVVGMADGFALASGAPALVNLHAAAGSGNAMG
ALTNAWYSHSPLVLTAGQQARSMIGVESMLANVDAPQLPRPLVKWSYEPACPEDVPRALSQAIHTACLPARGPVYVSIPH
DDWGHAVDDDAALLSARQVASAAQPAETQVEALVERLNAARNPVLVLGPDVDAYGANALAVTLAERLNAPAWVAPSASRC
PFPTRHACFRGVLPAAIQGISDRLDGHDLIVVIGAPVFRYHQYAPGRYLPDGAELVHLTCDMQEAARAPMGDALVGDIHA
MLDLLVPRVRQSGRARPEPQAAPEPQVDPEGMLTPETVFDVMNAMAPEDAIYVKESTSTVTAFWERVEMRHPGSYFFPAA
GGLGFGLPAAVGVQLACPERRVIGVIGDGSANYAITALWSAAQYRIPVVFVILKNGTYGALRWFSRLLDAEDSPGLDVPG
LDFCALAEGYGVEAEQAASREAFEAALKAALADDRPRVIEVPTTTIEP

Sequences:

>Translated_528_residues
MPTVHSATYDLLRRQGMTTIFGNPGSNELPFLKNFPADFRYILGLHEGVVVGMADGFALASGAPALVNLHAAAGSGNAMG
ALTNAWYSHSPLVLTAGQQARSMIGVESMLANVDAPQLPRPLVKWSYEPACPEDVPRALSQAIHTACLPARGPVYVSIPH
DDWGHAVDDDAALLSARQVASAAQPAETQVEALVERLNAARNPVLVLGPDVDAYGANALAVTLAERLNAPAWVAPSASRC
PFPTRHACFRGVLPAAIQGISDRLDGHDLIVVIGAPVFRYHQYAPGRYLPDGAELVHLTCDMQEAARAPMGDALVGDIHA
MLDLLVPRVRQSGRARPEPQAAPEPQVDPEGMLTPETVFDVMNAMAPEDAIYVKESTSTVTAFWERVEMRHPGSYFFPAA
GGLGFGLPAAVGVQLACPERRVIGVIGDGSANYAITALWSAAQYRIPVVFVILKNGTYGALRWFSRLLDAEDSPGLDVPG
LDFCALAEGYGVEAEQAASREAFEAALKAALADDRPRVIEVPTTTIEP
>Mature_527_residues
PTVHSATYDLLRRQGMTTIFGNPGSNELPFLKNFPADFRYILGLHEGVVVGMADGFALASGAPALVNLHAAAGSGNAMGA
LTNAWYSHSPLVLTAGQQARSMIGVESMLANVDAPQLPRPLVKWSYEPACPEDVPRALSQAIHTACLPARGPVYVSIPHD
DWGHAVDDDAALLSARQVASAAQPAETQVEALVERLNAARNPVLVLGPDVDAYGANALAVTLAERLNAPAWVAPSASRCP
FPTRHACFRGVLPAAIQGISDRLDGHDLIVVIGAPVFRYHQYAPGRYLPDGAELVHLTCDMQEAARAPMGDALVGDIHAM
LDLLVPRVRQSGRARPEPQAAPEPQVDPEGMLTPETVFDVMNAMAPEDAIYVKESTSTVTAFWERVEMRHPGSYFFPAAG
GLGFGLPAAVGVQLACPERRVIGVIGDGSANYAITALWSAAQYRIPVVFVILKNGTYGALRWFSRLLDAEDSPGLDVPGL
DFCALAEGYGVEAEQAASREAFEAALKAALADDRPRVIEVPTTTIEP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=525, Percent_Identity=22.0952380952381, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=26.4492753623188, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=28.0575539568345, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790104, Length=541, Percent_Identity=26.0628465804067, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787096, Length=484, Percent_Identity=21.4876033057851, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788716, Length=468, Percent_Identity=19.8717948717949, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=24.5045045045045, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 56185; Mature: 56054

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVHSATYDLLRRQGMTTIFGNPGSNELPFLKNFPADFRYILGLHEGVVVGMADGFALA
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCHHHHHHHHCCCCEEEEECCCEEEC
SGAPALVNLHAAAGSGNAMGALTNAWYSHSPLVLTAGQQARSMIGVESMLANVDAPQLPR
CCCCEEEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCC
PLVKWSYEPACPEDVPRALSQAIHTACLPARGPVYVSIPHDDWGHAVDDDAALLSARQVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
SAAQPAETQVEALVERLNAARNPVLVLGPDVDAYGANALAVTLAERLNAPAWVAPSASRC
HHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCC
PFPTRHACFRGVLPAAIQGISDRLDGHDLIVVIGAPVFRYHQYAPGRYLPDGAELVHLTC
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCEEEEEEE
DMQEAARAPMGDALVGDIHAMLDLLVPRVRQSGRARPEPQAAPEPQVDPEGMLTPETVFD
CHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VMNAMAPEDAIYVKESTSTVTAFWERVEMRHPGSYFFPAAGGLGFGLPAAVGVQLACPER
HHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHCEEEECCCC
RVIGVIGDGSANYAITALWSAAQYRIPVVFVILKNGTYGALRWFSRLLDAEDSPGLDVPG
EEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
LDFCALAEGYGVEAEQAASREAFEAALKAALADDRPRVIEVPTTTIEP
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
PTVHSATYDLLRRQGMTTIFGNPGSNELPFLKNFPADFRYILGLHEGVVVGMADGFALA
CCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCHHHHHHHHCCCCEEEEECCCEEEC
SGAPALVNLHAAAGSGNAMGALTNAWYSHSPLVLTAGQQARSMIGVESMLANVDAPQLPR
CCCCEEEEEEEECCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCC
PLVKWSYEPACPEDVPRALSQAIHTACLPARGPVYVSIPHDDWGHAVDDDAALLSARQVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
SAAQPAETQVEALVERLNAARNPVLVLGPDVDAYGANALAVTLAERLNAPAWVAPSASRC
HHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCC
PFPTRHACFRGVLPAAIQGISDRLDGHDLIVVIGAPVFRYHQYAPGRYLPDGAELVHLTC
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCEEEEEEE
DMQEAARAPMGDALVGDIHAMLDLLVPRVRQSGRARPEPQAAPEPQVDPEGMLTPETVFD
CHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VMNAMAPEDAIYVKESTSTVTAFWERVEMRHPGSYFFPAAGGLGFGLPAAVGVQLACPER
HHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHCEEEECCCC
RVIGVIGDGSANYAITALWSAAQYRIPVVFVILKNGTYGALRWFSRLLDAEDSPGLDVPG
EEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
LDFCALAEGYGVEAEQAASREAFEAALKAALADDRPRVIEVPTTTIEP
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]