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The map label for this gene is yiaO [H]

Identifier: 92112440

GI number: 92112440

Start: 345222

End: 346235

Strand: Direct

Name: yiaO [H]

Synonym: Csal_0306

Alternate gene names: 92112440

Gene position: 345222-346235 (Clockwise)

Preceding gene: 92112439

Following gene: 92112442

Centisome position: 9.34

GC content: 61.83

Gene sequence:

>1014_bases
ATGAAAACAATCTATTACGCCGCTGGCTTGACCCTTGGATTGGCCGTGATGCCTGGCCTGGCGCAGGCGGAAACCCTGCG
TCTGGCACACGTCTGGCCGGGGGGATCCAAGATCGACAAGGAAATCTTCCAGGCCTGGGCCGACAGCGTGGAAGAAGCCT
CGGACGGGCGCCTGGAGGTGCAGGTGTTCCCCGGCCAGACGCTCGCCAAGTCCGATGCCACCTACGAAGCCGTGGTCAAC
GGGATCGCCGATATCGGCGCCACGGCGCAGGGCTACAACTCGGGGCGCTTCCCTCTGACGCAGGTCGTCGAGGTGCCGGG
AGTCTCCAACAGCAGCCGGCAAGGCTCCTGCGTGTTGCAGAAGCTCTATGACCAGGGCGACCTTGACCAGGAATATTCGG
ACTCGCACGTGCTGTTCATGTTCACGACCGGTCCGGGTTACCTGCACGCCAAGGAAAAGCTTCTCGAAACGCCCAGCGAC
CTGGAAGGCATGCGGCTGCGCCGTCCCACCTCGCTGGTGGGCGACATGATGGCGGACCTGGGCGCCCAGCCCGTGGGCAT
GCCCGCGCCGGATATCTACACCGCCTTGCAGCGCGGCGTGATGGATGGCTTGAGCATCAACTGGGAAGGGGCCAAGACGT
TCCGGGTCAACGAGCTCGTCAACTACCATACCGAAGTGCCGCTGTACGATCTGGCACTGATCGCGACGATGAACAAGGAC
GACTACGCCAGCCTGCCGGATGACCTGAAGAAAGTCATCGATGACCACAGCGGCATGGAATGGTCGATGAAGGCGGCCAG
CGTCTACGACGAACTGATCGAGGAAGGACGCCAGGAAGCACTCGATGCGGGGGATGAAATCATCACCATCGAAAACCCGC
TCGAGAACGAGGACTGGGGCCCGGTACTCAAGGATACGATCAATCGTTATGTCAGCGATCTGCAGGGCAAGGGCCTGCCG
GGCGCGCAGACCTACGACAATGTCATGACCCTCAAGGCGTCTTGCGACGCCTGA

Upstream 100 bases:

>100_bases
CCGTACTCAGTCTGTTTCTCATCTGATTCCAGGAGTGGAACGCGCGAGTGGTATGAAAGGACACAAACGCTAGCCATCAA
CACATCATGAGGTACCAACA

Downstream 100 bases:

>100_bases
TAGGGAGGCTCTGATTCCTCCGGAGGCGCCCTGACGCCTAGGGCGTTTCCGGCGGGACCGTCTCGTCATGATGACGGCTT
GTCGCTTCGATGAGCGTGCG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKTIYYAAGLTLGLAVMPGLAQAETLRLAHVWPGGSKIDKEIFQAWADSVEEASDGRLEVQVFPGQTLAKSDATYEAVVN
GIADIGATAQGYNSGRFPLTQVVEVPGVSNSSRQGSCVLQKLYDQGDLDQEYSDSHVLFMFTTGPGYLHAKEKLLETPSD
LEGMRLRRPTSLVGDMMADLGAQPVGMPAPDIYTALQRGVMDGLSINWEGAKTFRVNELVNYHTEVPLYDLALIATMNKD
DYASLPDDLKKVIDDHSGMEWSMKAASVYDELIEEGRQEALDAGDEIITIENPLENEDWGPVLKDTINRYVSDLQGKGLP
GAQTYDNVMTLKASCDA

Sequences:

>Translated_337_residues
MKTIYYAAGLTLGLAVMPGLAQAETLRLAHVWPGGSKIDKEIFQAWADSVEEASDGRLEVQVFPGQTLAKSDATYEAVVN
GIADIGATAQGYNSGRFPLTQVVEVPGVSNSSRQGSCVLQKLYDQGDLDQEYSDSHVLFMFTTGPGYLHAKEKLLETPSD
LEGMRLRRPTSLVGDMMADLGAQPVGMPAPDIYTALQRGVMDGLSINWEGAKTFRVNELVNYHTEVPLYDLALIATMNKD
DYASLPDDLKKVIDDHSGMEWSMKAASVYDELIEEGRQEALDAGDEIITIENPLENEDWGPVLKDTINRYVSDLQGKGLP
GAQTYDNVMTLKASCDA
>Mature_337_residues
MKTIYYAAGLTLGLAVMPGLAQAETLRLAHVWPGGSKIDKEIFQAWADSVEEASDGRLEVQVFPGQTLAKSDATYEAVVN
GIADIGATAQGYNSGRFPLTQVVEVPGVSNSSRQGSCVLQKLYDQGDLDQEYSDSHVLFMFTTGPGYLHAKEKLLETPSD
LEGMRLRRPTSLVGDMMADLGAQPVGMPAPDIYTALQRGVMDGLSINWEGAKTFRVNELVNYHTEVPLYDLALIATMNKD
DYASLPDDLKKVIDDHSGMEWSMKAASVYDELIEEGRQEALDAGDEIITIENPLENEDWGPVLKDTINRYVSDLQGKGLP
GAQTYDNVMTLKASCDA

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=303, Percent_Identity=22.7722772277228, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36764; Mature: 36764

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIYYAAGLTLGLAVMPGLAQAETLRLAHVWPGGSKIDKEIFQAWADSVEEASDGRLEV
CCEEEEECHHHHHHHHCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
QVFPGQTLAKSDATYEAVVNGIADIGATAQGYNSGRFPLTQVVEVPGVSNSSRQGSCVLQ
EECCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCCHHHHHH
KLYDQGDLDQEYSDSHVLFMFTTGPGYLHAKEKLLETPSDLEGMRLRRPTSLVGDMMADL
HHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
GAQPVGMPAPDIYTALQRGVMDGLSINWEGAKTFRVNELVNYHTEVPLYDLALIATMNKD
CCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEEHHHHHHHHCCCCHHHEEEEEECCCC
DYASLPDDLKKVIDDHSGMEWSMKAASVYDELIEEGRQEALDAGDEIITIENPLENEDWG
CCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
PVLKDTINRYVSDLQGKGLPGAQTYDNVMTLKASCDA
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MKTIYYAAGLTLGLAVMPGLAQAETLRLAHVWPGGSKIDKEIFQAWADSVEEASDGRLEV
CCEEEEECHHHHHHHHCCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
QVFPGQTLAKSDATYEAVVNGIADIGATAQGYNSGRFPLTQVVEVPGVSNSSRQGSCVLQ
EECCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCCHHHHHH
KLYDQGDLDQEYSDSHVLFMFTTGPGYLHAKEKLLETPSDLEGMRLRRPTSLVGDMMADL
HHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
GAQPVGMPAPDIYTALQRGVMDGLSINWEGAKTFRVNELVNYHTEVPLYDLALIATMNKD
CCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEEHHHHHHHHCCCCHHHEEEEEECCCC
DYASLPDDLKKVIDDHSGMEWSMKAASVYDELIEEGRQEALDAGDEIITIENPLENEDWG
CCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
PVLKDTINRYVSDLQGKGLPGAQTYDNVMTLKASCDA
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]