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The map label for this gene is glnG [H]

Identifier: 92112381

GI number: 92112381

Start: 279942

End: 281351

Strand: Direct

Name: glnG [H]

Synonym: Csal_0246

Alternate gene names: 92112381

Gene position: 279942-281351 (Clockwise)

Preceding gene: 92112380

Following gene: 92112382

Centisome position: 7.57

GC content: 68.37

Gene sequence:

>1410_bases
ATGACTGACGTCGCACGCATCATGATCGTCGACGACGATCGCGCCATTCGCTGGGTGCTGGAGCGCGCGCTGGCTCAGCC
GGACCTCGAGGTCGAGAGCTTCGAGCGTGCCGATAGTGCCCTGGAGCGCATCCTGCGACAGCCACCGGATGTCCTGTTGA
CCGATATCCGCATGCCGGGGCTGGACGGGCTGGACCTGATGGCGCGCATCCGTGAAAAGTATCCGGATCTGCCGGTGATC
GTGATGACCGCCCACTCCGATCTCGACAGCGCGGTGGCCTCGTATCAGGGCGGTGCCTTCGAGTACCTGCCCAAGCCGTT
CGACGTCGACGAGGCGCTGGCGCTGGTGCGGCGCGGCGTGGCGCATGCCCGCGAGCGGCGCACGCCGGCGGCGGTGCCCG
AAGGACTGTCGGCAGAGATCATCGGTGAAGCCCCGGCGATGCAGGAGGTCTTCCGTGCCATCGGCCGGCTCTCGCAATCC
CACATCACGGTGATGATCAACGGCGAGTCGGGGACCGGCAAGGAGCGCGTCGCCCAGGCGCTGCACCAGCACAGCCCGCG
ACACGCCGCGCCGTTCATCGCGCTCAACATGGCGGCGATTCCCAAGGACCTGATGGAATCGGAACTGTTCGGTCATGAAA
AGGGCGCCTTCACCGGGGCGACCTCGCAGCGGCGCGGGCGTTTCGAACAGGCCGATGGCGGCACGCTGTTCCTCGACGAG
ATCGGCGACATGCCCGCCGAGACCCAGACCCGCCTGCTGCGGGTGCTGGCCGACGGAGAGTTCTACCGGGTCGGGGGGCA
TACGCCGGTCAAGGTCGATGTTCGCATCATCGCCGCGACCCACCAGCATCTGGAGCGGCTGGTCGAGGACGGCCGTTTCC
GCGAGGACCTGTTCCACCGCCTCAACGTGATCCGCGTGCACCTGCCGCGTCTGGCAGAGCGCCGCGAGGACATTCCGCGT
CTGGCCCAGCACTTCCTCGCCGAGGCCGCCAAGGAGCTTGCCACCGAACCCAAGGTGCTGACCCAAGAGGCCGAGCAGCA
TCTCACCCGCCTGCCATGGCCGGGCAACGTGCGCCAGCTCGAGAATACCTGCCGCTGGCTGACGGTGATGGCCTCGGGGC
GCGAGGTGTTGATCGAGGACCTGCCGCCGGAGCTGCGCGATGTCGAAGGCACCGAAAGCGCCGGCGGCGACTGGCGCGGC
GCCTTCCGCGAGTGGGCCGATCGCGCCCTGGCCGCCGGGCATACGCACCTGCTCGAGGAGGCGGTGCCGGATTTCGAGCG
CATCCTCATCGAGACGGCGCTCAAGCACACCGGCGGGCGCAAGGGCGAAGCCGCCGAGCTGCTGGGCTGGGGGCGCAATA
CACTGACGCGTAAGCTCAAGGTGCTGCTGCCGGCGCTGGCCGACGAATGA

Upstream 100 bases:

>100_bases
TCAGCACCAGGGGTTGATCGAGTGCGACTCGGTGCCCGGGTGTACCGAATTCCGCTTGTTGATTCCGCTTGAGAGCCATC
ATTGAGGCTTGGGAGGCCCC

Downstream 100 bases:

>100_bases
CCCACGACGGCGCGGGGCGTCGAGGCGCGGTCATGCCGACGTCACGGGAACGTCATCGAACGGACGCGGCATTGTCATTC
GCTGGACCGAACAATGGGCA

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTDVARIMIVDDDRAIRWVLERALAQPDLEVESFERADSALERILRQPPDVLLTDIRMPGLDGLDLMARIREKYPDLPVI
VMTAHSDLDSAVASYQGGAFEYLPKPFDVDEALALVRRGVAHARERRTPAAVPEGLSAEIIGEAPAMQEVFRAIGRLSQS
HITVMINGESGTGKERVAQALHQHSPRHAAPFIALNMAAIPKDLMESELFGHEKGAFTGATSQRRGRFEQADGGTLFLDE
IGDMPAETQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQHLERLVEDGRFREDLFHRLNVIRVHLPRLAERREDIPR
LAQHFLAEAAKELATEPKVLTQEAEQHLTRLPWPGNVRQLENTCRWLTVMASGREVLIEDLPPELRDVEGTESAGGDWRG
AFREWADRALAAGHTHLLEEAVPDFERILIETALKHTGGRKGEAAELLGWGRNTLTRKLKVLLPALADE

Sequences:

>Translated_469_residues
MTDVARIMIVDDDRAIRWVLERALAQPDLEVESFERADSALERILRQPPDVLLTDIRMPGLDGLDLMARIREKYPDLPVI
VMTAHSDLDSAVASYQGGAFEYLPKPFDVDEALALVRRGVAHARERRTPAAVPEGLSAEIIGEAPAMQEVFRAIGRLSQS
HITVMINGESGTGKERVAQALHQHSPRHAAPFIALNMAAIPKDLMESELFGHEKGAFTGATSQRRGRFEQADGGTLFLDE
IGDMPAETQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQHLERLVEDGRFREDLFHRLNVIRVHLPRLAERREDIPR
LAQHFLAEAAKELATEPKVLTQEAEQHLTRLPWPGNVRQLENTCRWLTVMASGREVLIEDLPPELRDVEGTESAGGDWRG
AFREWADRALAAGHTHLLEEAVPDFERILIETALKHTGGRKGEAAELLGWGRNTLTRKLKVLLPALADE
>Mature_468_residues
TDVARIMIVDDDRAIRWVLERALAQPDLEVESFERADSALERILRQPPDVLLTDIRMPGLDGLDLMARIREKYPDLPVIV
MTAHSDLDSAVASYQGGAFEYLPKPFDVDEALALVRRGVAHARERRTPAAVPEGLSAEIIGEAPAMQEVFRAIGRLSQSH
ITVMINGESGTGKERVAQALHQHSPRHAAPFIALNMAAIPKDLMESELFGHEKGAFTGATSQRRGRFEQADGGTLFLDEI
GDMPAETQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQHLERLVEDGRFREDLFHRLNVIRVHLPRLAERREDIPRL
AQHFLAEAAKELATEPKVLTQEAEQHLTRLPWPGNVRQLENTCRWLTVMASGREVLIEDLPPELRDVEGTESAGGDWRGA
FREWADRALAAGHTHLLEEAVPDFERILIETALKHTGGRKGEAAELLGWGRNTLTRKLKVLLPALADE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=461, Percent_Identity=68.5466377440347, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=41.505376344086, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=40.5701754385965, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1788905, Length=378, Percent_Identity=42.5925925925926, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87082117, Length=331, Percent_Identity=42.5981873111783, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=48.7704918032787, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=42.2018348623853, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=41.4925373134328, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=41.8238993710692, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=270, Percent_Identity=40.7407407407407, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=46.0176991150442, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=31.023102310231, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789828, Length=269, Percent_Identity=35.3159851301115, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=32.4786324786325, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790860, Length=126, Percent_Identity=31.7460317460317, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789402, Length=122, Percent_Identity=31.9672131147541, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52361; Mature: 52230

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVARIMIVDDDRAIRWVLERALAQPDLEVESFERADSALERILRQPPDVLLTDIRMPG
CCCCEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LDGLDLMARIREKYPDLPVIVMTAHSDLDSAVASYQGGAFEYLPKPFDVDEALALVRRGV
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
AHARERRTPAAVPEGLSAEIIGEAPAMQEVFRAIGRLSQSHITVMINGESGTGKERVAQA
HHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LHQHSPRHAAPFIALNMAAIPKDLMESELFGHEKGAFTGATSQRRGRFEQADGGTLFLDE
HHHCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEHHH
IGDMPAETQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQHLERLVEDGRFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHH
LNVIRVHLPRLAERREDIPRLAQHFLAEAAKELATEPKVLTQEAEQHLTRLPWPGNVRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGREVLIEDLPPELRDVEGTESAGGDWRGAFREWADRALAAGHTHLLEE
HHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVPDFERILIETALKHTGGRKGEAAELLGWGRNTLTRKLKVLLPALADE
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDVARIMIVDDDRAIRWVLERALAQPDLEVESFERADSALERILRQPPDVLLTDIRMPG
CCCEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LDGLDLMARIREKYPDLPVIVMTAHSDLDSAVASYQGGAFEYLPKPFDVDEALALVRRGV
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
AHARERRTPAAVPEGLSAEIIGEAPAMQEVFRAIGRLSQSHITVMINGESGTGKERVAQA
HHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LHQHSPRHAAPFIALNMAAIPKDLMESELFGHEKGAFTGATSQRRGRFEQADGGTLFLDE
HHHCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEHHH
IGDMPAETQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQHLERLVEDGRFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHH
LNVIRVHLPRLAERREDIPRLAQHFLAEAAKELATEPKVLTQEAEQHLTRLPWPGNVRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGREVLIEDLPPELRDVEGTESAGGDWRGAFREWADRALAAGHTHLLEE
HHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVPDFERILIETALKHTGGRKGEAAELLGWGRNTLTRKLKVLLPALADE
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]