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The map label for this gene is ptxB [H]

Identifier: 92112382

GI number: 92112382

Start: 281484

End: 282458

Strand: Direct

Name: ptxB [H]

Synonym: Csal_0247

Alternate gene names: 92112382

Gene position: 281484-282458 (Clockwise)

Preceding gene: 92112381

Following gene: 92112383

Centisome position: 7.61

GC content: 63.79

Gene sequence:

>975_bases
ATGTTCTCGCAGACGCTTCCTCTGACCCGCCCCGCCCTGGGGCTTGCCTTCGCTGCCCTGGTCGCCGGGGTGTCCACCGC
CCAGGCCGATTTCCAGCTCAGTGAACGCTACACCGATGCCGATGGCGACCTCGTCGCCGACGTGCCGGAAGACGAATCCC
AGTGGCGCGATCCGGACACGCTGGTCTTCGCCTATACCCCGGTGGAAGATCCTGCCGTCTACGCGGACGTGTGGTCCGGC
TTCCTGGATCACATGGAGGAGGTCACCGGCAAGAAAGTGCGCTTCTTCCCCGTGCAGTCCAATGCCGCGCAGATCGAGGC
GATGCGCGCCGGCCGCCTGCACGTGGCCGGTTTCAACACCGGTTCCAACCCGCTGGCGGTGGCCTGCGCGGGCTTTCGGC
CCTTCGCGATGATGGCCGCCGAGGATGGCTCCTTCGGCTACGAGATGGAGATCATCACCTACCCCGGTTCGGGCATCGAC
GAGGTCGAGGACATCAAGGGCAAGACCCTGACCCATACCTCGGAGACGTCCAATTCCGGCTACAAGGCGCCCACCGCGCT
GATGGCCTCGGAATTCGGCATGACCTCCGGCGAGGATTACGAGGTCGACTTCTCCGGCAAGCACGACAATTCGATCCTGG
GCGTGGCCAATCACGACTATGAAGTCGCCACCATCGCCAACTCGGTCAAGGCGCGCATGCTCGAGCGCGACGTGATCGAC
GCGGATCAGATCGAGGTCATCTACCAGTCGGATACCTTCCCGACCACGGCGTACGGGGTGGCCCACGACCTCGCGCCCGA
GCTGCAGGAAGCGATCAAGGAAGCCTTCTTCGGCTTCGACTGGGAGGGCTCCGCGCTGGCCGAGGAATTCGGCAAGAACG
GCGAGTCGCAGTTCATTCCCATCACCTTCAAGAAACACTGGTCGGTGATCCGCCAGATCGACGCTGCCAACGGCGTCGAC
TACCAGTGCTCGTAA

Upstream 100 bases:

>100_bases
ATGCCGACGTCACGGGAACGTCATCGAACGGACGCGGCATTGTCATTCGCTGGACCGAACAATGGGCATCGCTCATTCGT
TCGCAACCAGGTGACAACGC

Downstream 100 bases:

>100_bases
GCGCCGACTAATCGCGCGCGACGGGTGGGCCGGGCGTTGCCACGCGACGCTCGGCCCGTTTTGGCAGGGAGATGGATGTG
CTGGAACTACAACGACTCAC

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MFSQTLPLTRPALGLAFAALVAGVSTAQADFQLSERYTDADGDLVADVPEDESQWRDPDTLVFAYTPVEDPAVYADVWSG
FLDHMEEVTGKKVRFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPLAVACAGFRPFAMMAAEDGSFGYEMEIITYPGSGID
EVEDIKGKTLTHTSETSNSGYKAPTALMASEFGMTSGEDYEVDFSGKHDNSILGVANHDYEVATIANSVKARMLERDVID
ADQIEVIYQSDTFPTTAYGVAHDLAPELQEAIKEAFFGFDWEGSALAEEFGKNGESQFIPITFKKHWSVIRQIDAANGVD
YQCS

Sequences:

>Translated_324_residues
MFSQTLPLTRPALGLAFAALVAGVSTAQADFQLSERYTDADGDLVADVPEDESQWRDPDTLVFAYTPVEDPAVYADVWSG
FLDHMEEVTGKKVRFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPLAVACAGFRPFAMMAAEDGSFGYEMEIITYPGSGID
EVEDIKGKTLTHTSETSNSGYKAPTALMASEFGMTSGEDYEVDFSGKHDNSILGVANHDYEVATIANSVKARMLERDVID
ADQIEVIYQSDTFPTTAYGVAHDLAPELQEAIKEAFFGFDWEGSALAEEFGKNGESQFIPITFKKHWSVIRQIDAANGVD
YQCS
>Mature_324_residues
MFSQTLPLTRPALGLAFAALVAGVSTAQADFQLSERYTDADGDLVADVPEDESQWRDPDTLVFAYTPVEDPAVYADVWSG
FLDHMEEVTGKKVRFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPLAVACAGFRPFAMMAAEDGSFGYEMEIITYPGSGID
EVEDIKGKTLTHTSETSNSGYKAPTALMASEFGMTSGEDYEVDFSGKHDNSILGVANHDYEVATIANSVKARMLERDVID
ADQIEVIYQSDTFPTTAYGVAHDLAPELQEAIKEAFFGFDWEGSALAEEFGKNGESQFIPITFKKHWSVIRQIDAANGVD
YQCS

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=256, Percent_Identity=24.609375, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSQTLPLTRPALGLAFAALVAGVSTAQADFQLSERYTDADGDLVADVPEDESQWRDPDT
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCEEHHHHCCCCCCCEEEECCCCHHHCCCCCE
LVFAYTPVEDPAVYADVWSGFLDHMEEVTGKKVRFFPVQSNAAQIEAMRAGRLHVAGFNT
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEECCC
GSNPLAVACAGFRPFAMMAAEDGSFGYEMEIITYPGSGIDEVEDIKGKTLTHTSETSNSG
CCCCEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHCCCCEEEECCCCCCCC
YKAPTALMASEFGMTSGEDYEVDFSGKHDNSILGVANHDYEVATIANSVKARMLERDVID
CCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHCCC
ADQIEVIYQSDTFPTTAYGVAHDLAPELQEAIKEAFFGFDWEGSALAEEFGKNGESQFIP
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEE
ITFKKHWSVIRQIDAANGVDYQCS
EEHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFSQTLPLTRPALGLAFAALVAGVSTAQADFQLSERYTDADGDLVADVPEDESQWRDPDT
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHCEEHHHHCCCCCCCEEEECCCCHHHCCCCCE
LVFAYTPVEDPAVYADVWSGFLDHMEEVTGKKVRFFPVQSNAAQIEAMRAGRLHVAGFNT
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEECCC
GSNPLAVACAGFRPFAMMAAEDGSFGYEMEIITYPGSGIDEVEDIKGKTLTHTSETSNSG
CCCCEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHCCCCEEEECCCCCCCC
YKAPTALMASEFGMTSGEDYEVDFSGKHDNSILGVANHDYEVATIANSVKARMLERDVID
CCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHCCC
ADQIEVIYQSDTFPTTAYGVAHDLAPELQEAIKEAFFGFDWEGSALAEEFGKNGESQFIP
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEE
ITFKKHWSVIRQIDAANGVDYQCS
EEHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]