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The map label for this gene is glnL [H]

Identifier: 92112380

GI number: 92112380

Start: 278829

End: 279926

Strand: Direct

Name: glnL [H]

Synonym: Csal_0245

Alternate gene names: 92112380

Gene position: 278829-279926 (Clockwise)

Preceding gene: 92112379

Following gene: 92112381

Centisome position: 7.54

GC content: 66.3

Gene sequence:

>1098_bases
ATGACGATGGATAATGGACGGGCCATGCCGGAACCTTCCCAGCAGCAGTTTCTCGAACAGCAGCGCCTGCTCGAACATTT
GACGACCGCCGTGGTACTGCTCGACGGCGAGCTGCGGCTGCGTTGGATGAACCCCGCGGCCGAGTCGCTGCTGGCGACCA
GCCGGGTGCGGGTCGTCGGCCAGTGTCTGGAGAGCCTGGCGGGCGTCGAGGGCAGTGTCGAGGCGGTGTTGATCAAGGCG
CGCGACGAGCTGCATCCCTTCACGCAGCGCGAGGCGACGCTGGTATTGCCGTCGGGGGATACGGCCACGGTGGACTTCAC
CGTCACGCCGTTGTCCGCCGATGAACTGCTGATCGAGATCGAGCCGCGCGACCGGCTGTTGCGCATTTCCCGCGAGGAGG
CGCTGATCGCGCGCCAGGAGACCATCAAGGTGCTCACCCGGGGGCTGGCGCACGAGGTGAAGAACCCGCTGGGCGGCATT
CGCGGTGCGGCGCAGTTGCTCGAGCGAGACCTGCCCGATCCGCAGCTCGCCGAGTTCACCCGGGTCATCGTCGAAGAGGC
CGATCGGCTGCGCGATCTGGTGGATCGCATGCTGGGGCCCAACAGCATGAGTCGTCACGAGCCGCTCAACATTCACAAGG
TGCTCGAGCGGGTGCGTACCCTGCTGGAGGCCGAACATCCGCGCATCGACTACGTGCGCGATTACGACCCCAGCCTGCCG
GATCTGGAAGGTGACGAAGCGCAGCTGATTCAGGCGGTGCTCAACGTCGCCCGCAACGCCGTGCAGGCCATGGAGGAACA
CGGCGTCGAGACGCCCCGGCTGATTCTGCGCACGCGGGCGCGACGCCAGTTCACGCTGGGCGCCGAGCGCCACCGGCTGG
TCTGCGAGCTGGGCATTCTCGACAACGGCCCCGGCATTCCCGAGACGCTGCGCGAGACGCTGTTCTATCCGATGGTGTCG
GGGCGTGCCGAGGGCAGTGGCCTGGGGCTGTCCATCGCCCAGTCGATCCTGCATCAGCACCAGGGGTTGATCGAGTGCGA
CTCGGTGCCCGGGTGTACCGAATTCCGCTTGTTGATTCCGCTTGAGAGCCATCATTGA

Upstream 100 bases:

>100_bases
TTTGCACTAATTTGGTGCATGGTGGCGTCATCCCGTGTCGGCTGCACTGCCGTATGCCGGGCCGATCAGGACTGGCGCGC
TTCTTGCACTGTTAACGGAC

Downstream 100 bases:

>100_bases
GGCTTGGGAGGCCCCATGACTGACGTCGCACGCATCATGATCGTCGACGACGATCGCGCCATTCGCTGGGTGCTGGAGCG
CGCGCTGGCTCAGCCGGACC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTMDNGRAMPEPSQQQFLEQQRLLEHLTTAVVLLDGELRLRWMNPAAESLLATSRVRVVGQCLESLAGVEGSVEAVLIKA
RDELHPFTQREATLVLPSGDTATVDFTVTPLSADELLIEIEPRDRLLRISREEALIARQETIKVLTRGLAHEVKNPLGGI
RGAAQLLERDLPDPQLAEFTRVIVEEADRLRDLVDRMLGPNSMSRHEPLNIHKVLERVRTLLEAEHPRIDYVRDYDPSLP
DLEGDEAQLIQAVLNVARNAVQAMEEHGVETPRLILRTRARRQFTLGAERHRLVCELGILDNGPGIPETLRETLFYPMVS
GRAEGSGLGLSIAQSILHQHQGLIECDSVPGCTEFRLLIPLESHH

Sequences:

>Translated_365_residues
MTMDNGRAMPEPSQQQFLEQQRLLEHLTTAVVLLDGELRLRWMNPAAESLLATSRVRVVGQCLESLAGVEGSVEAVLIKA
RDELHPFTQREATLVLPSGDTATVDFTVTPLSADELLIEIEPRDRLLRISREEALIARQETIKVLTRGLAHEVKNPLGGI
RGAAQLLERDLPDPQLAEFTRVIVEEADRLRDLVDRMLGPNSMSRHEPLNIHKVLERVRTLLEAEHPRIDYVRDYDPSLP
DLEGDEAQLIQAVLNVARNAVQAMEEHGVETPRLILRTRARRQFTLGAERHRLVCELGILDNGPGIPETLRETLFYPMVS
GRAEGSGLGLSIAQSILHQHQGLIECDSVPGCTEFRLLIPLESHH
>Mature_364_residues
TMDNGRAMPEPSQQQFLEQQRLLEHLTTAVVLLDGELRLRWMNPAAESLLATSRVRVVGQCLESLAGVEGSVEAVLIKAR
DELHPFTQREATLVLPSGDTATVDFTVTPLSADELLIEIEPRDRLLRISREEALIARQETIKVLTRGLAHEVKNPLGGIR
GAAQLLERDLPDPQLAEFTRVIVEEADRLRDLVDRMLGPNSMSRHEPLNIHKVLERVRTLLEAEHPRIDYVRDYDPSLPD
LEGDEAQLIQAVLNVARNAVQAMEEHGVETPRLILRTRARRQFTLGAERHRLVCELGILDNGPGIPETLRETLFYPMVSG
RAEGSGLGLSIAQSILHQHQGLIECDSVPGCTEFRLLIPLESHH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=341, Percent_Identity=46.0410557184751, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI1788549, Length=356, Percent_Identity=28.0898876404494, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=27.8008298755187, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40834; Mature: 40703

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDNGRAMPEPSQQQFLEQQRLLEHLTTAVVLLDGELRLRWMNPAAESLLATSRVRVVG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCHHHHHHHHHHHHHHHH
QCLESLAGVEGSVEAVLIKARDELHPFTQREATLVLPSGDTATVDFTVTPLSADELLIEI
HHHHHHHCCCCCEEEEEEECHHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEE
EPRDRLLRISREEALIARQETIKVLTRGLAHEVKNPLGGIRGAAQLLERDLPDPQLAEFT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVIVEEADRLRDLVDRMLGPNSMSRHEPLNIHKVLERVRTLLEAEHPRIDYVRDYDPSLP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DLEGDEAQLIQAVLNVARNAVQAMEEHGVETPRLILRTRARRQFTLGAERHRLVCELGIL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
DNGPGIPETLRETLFYPMVSGRAEGSGLGLSIAQSILHQHQGLIECDSVPGCTEFRLLIP
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEEE
LESHH
ECCCC
>Mature Secondary Structure 
TMDNGRAMPEPSQQQFLEQQRLLEHLTTAVVLLDGELRLRWMNPAAESLLATSRVRVVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCHHHHHHHHHHHHHHHH
QCLESLAGVEGSVEAVLIKARDELHPFTQREATLVLPSGDTATVDFTVTPLSADELLIEI
HHHHHHHCCCCCEEEEEEECHHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEE
EPRDRLLRISREEALIARQETIKVLTRGLAHEVKNPLGGIRGAAQLLERDLPDPQLAEFT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RVIVEEADRLRDLVDRMLGPNSMSRHEPLNIHKVLERVRTLLEAEHPRIDYVRDYDPSLP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DLEGDEAQLIQAVLNVARNAVQAMEEHGVETPRLILRTRARRQFTLGAERHRLVCELGIL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCC
DNGPGIPETLRETLFYPMVSGRAEGSGLGLSIAQSILHQHQGLIECDSVPGCTEFRLLIP
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEEE
LESHH
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]