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The map label for this gene is gshB [H]

Identifier: 92112192

GI number: 92112192

Start: 76845

End: 77798

Strand: Direct

Name: gshB [H]

Synonym: Csal_0057

Alternate gene names: 92112192

Gene position: 76845-77798 (Clockwise)

Preceding gene: 92112191

Following gene: 92112193

Centisome position: 2.08

GC content: 66.35

Gene sequence:

>954_bases
ATGAGCGAGCGCGCCTTGAAGCTCGGCGTGGTGATGGACCCCATCGCCGATATCGCCTACAAGAAAGACACCACCCTGGC
CATGCTGTGGGCCGCCCAGGAGCGCGGCTGGTCGCTGTATTACCTCGAGCCCGAAGACCTGTACCTGGAAGGCGGACGCG
CCATGGGTCGCATGCGCGCGCTGGAAGCCTATCGCGACCCCGAGGCCTGGTACCGGCTCGACGTATCGCGGGCCGCGCCG
CTGGCGGAGCTCGACGTGATCCTGATGCGCCAGGACCCGCCGGTGGACGGCCATTTCCTCAATGCCGTGCATTTGCTGGG
CTTCGCCGAGCGCGAGGGCGTGCTGGTGGTCAACCCGACGCAGGCGCTGTTGACCAGCAACGAGAAACTCTTCGCACAGC
AGTTCCCGCAGTGCATTCCGCCCTCCGTGGTGTCCTGTCACGACGGCGTGCTGCGCGACTTCCACGCCCGGCACGGCGAC
GTGATCCTCAAGCCGCTGGATGGCATGGGCGGCAGCGGTATCTTCCACATCACGCCGGAAGGCCGGAACCTGGGCTCGGT
CATCGAGCAGCTCACCGAGCGCGGCACGCGCCAGATCATGGCGCAACGCTATCTGCCCGAGATCCAGGCCGGCGACACGC
GCATCCTGCTGATCGACGGCGAGCCGGTGCCCTATGGGCTGGCGCGCATTCCCAGCGCCGGCGAGACGCGTGGCAACCTG
GCGGCCGGCGGCCGTGGCGAGGCTCGCGAACTCACCGACCGCGACCATTGGCTGATCGAGCAGGTCAAGCCGGCGATTCT
CGAGAAGGGACTGATCTTCGTGGGGCTCGACGTGATCGGCGATTACATCACCGAGATCAATGTCACCAGTCCCACTTGCG
TGCGCGAAATCGACGCGCAGCGCGGCACGGATATCGGTGGTCAATTGATGGAGGCCATCGCCCGGCGCTTTTGA

Upstream 100 bases:

>100_bases
CGACGACGCTTCGTCCGACTGAGGCGTCGCCGGCGTCTTGGTCTTCCGTTGACGGCTGCGGCATACTGCGCGCATCCATT
CCTTACCCGGAAGACCGATC

Downstream 100 bases:

>100_bases
GCGGGACGCGCCGTCGCATGATGGCGAGCCCCGTGCCGGGGAGTGATACAATCGCCGTGAAAGGCACCAATTTCGGCGTG
CGCTAGAGGCGAGGCGCGCC

Product: glutathione synthase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSERALKLGVVMDPIADIAYKKDTTLAMLWAAQERGWSLYYLEPEDLYLEGGRAMGRMRALEAYRDPEAWYRLDVSRAAP
LAELDVILMRQDPPVDGHFLNAVHLLGFAEREGVLVVNPTQALLTSNEKLFAQQFPQCIPPSVVSCHDGVLRDFHARHGD
VILKPLDGMGGSGIFHITPEGRNLGSVIEQLTERGTRQIMAQRYLPEIQAGDTRILLIDGEPVPYGLARIPSAGETRGNL
AAGGRGEARELTDRDHWLIEQVKPAILEKGLIFVGLDVIGDYITEINVTSPTCVREIDAQRGTDIGGQLMEAIARRF

Sequences:

>Translated_317_residues
MSERALKLGVVMDPIADIAYKKDTTLAMLWAAQERGWSLYYLEPEDLYLEGGRAMGRMRALEAYRDPEAWYRLDVSRAAP
LAELDVILMRQDPPVDGHFLNAVHLLGFAEREGVLVVNPTQALLTSNEKLFAQQFPQCIPPSVVSCHDGVLRDFHARHGD
VILKPLDGMGGSGIFHITPEGRNLGSVIEQLTERGTRQIMAQRYLPEIQAGDTRILLIDGEPVPYGLARIPSAGETRGNL
AAGGRGEARELTDRDHWLIEQVKPAILEKGLIFVGLDVIGDYITEINVTSPTCVREIDAQRGTDIGGQLMEAIARRF
>Mature_316_residues
SERALKLGVVMDPIADIAYKKDTTLAMLWAAQERGWSLYYLEPEDLYLEGGRAMGRMRALEAYRDPEAWYRLDVSRAAPL
AELDVILMRQDPPVDGHFLNAVHLLGFAEREGVLVVNPTQALLTSNEKLFAQQFPQCIPPSVVSCHDGVLRDFHARHGDV
ILKPLDGMGGSGIFHITPEGRNLGSVIEQLTERGTRQIMAQRYLPEIQAGDTRILLIDGEPVPYGLARIPSAGETRGNLA
AGGRGEARELTDRDHWLIEQVKPAILEKGLIFVGLDVIGDYITEINVTSPTCVREIDAQRGTDIGGQLMEAIARRF

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=312, Percent_Identity=54.1666666666667, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35094; Mature: 34963

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERALKLGVVMDPIADIAYKKDTTLAMLWAAQERGWSLYYLEPEDLYLEGGRAMGRMRA
CCCCCEEECCHHHHHHHHHCCCCCEEEEEEEHHCCCCEEEEECCCCEEEECCHHHHHHHH
LEAYRDPEAWYRLDVSRAAPLAELDVILMRQDPPVDGHFLNAVHLLGFAEREGVLVVNPT
HHHHCCCHHHEEEEHHHCCCHHHEEEEEEECCCCCCCHHHHHHHHHEECCCCCEEEECCH
QALLTSNEKLFAQQFPQCIPPSVVSCHDGVLRDFHARHGDVILKPLDGMGGSGIFHITPE
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCC
GRNLGSVIEQLTERGTRQIMAQRYLPEIQAGDTRILLIDGEPVPYGLARIPSAGETRGNL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCC
AAGGRGEARELTDRDHWLIEQVKPAILEKGLIFVGLDVIGDYITEINVTSPTCVREIDAQ
CCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHEECCCCCHHHHHHHHH
RGTDIGGQLMEAIARRF
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SERALKLGVVMDPIADIAYKKDTTLAMLWAAQERGWSLYYLEPEDLYLEGGRAMGRMRA
CCCCEEECCHHHHHHHHHCCCCCEEEEEEEHHCCCCEEEEECCCCEEEECCHHHHHHHH
LEAYRDPEAWYRLDVSRAAPLAELDVILMRQDPPVDGHFLNAVHLLGFAEREGVLVVNPT
HHHHCCCHHHEEEEHHHCCCHHHEEEEEEECCCCCCCHHHHHHHHHEECCCCCEEEECCH
QALLTSNEKLFAQQFPQCIPPSVVSCHDGVLRDFHARHGDVILKPLDGMGGSGIFHITPE
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCC
GRNLGSVIEQLTERGTRQIMAQRYLPEIQAGDTRILLIDGEPVPYGLARIPSAGETRGNL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCC
AAGGRGEARELTDRDHWLIEQVKPAILEKGLIFVGLDVIGDYITEINVTSPTCVREIDAQ
CCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHEECCCCCHHHHHHHHH
RGTDIGGQLMEAIARRF
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]