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The map label for this gene is dnaN [H]

Identifier: 92112138

GI number: 92112138

Start: 1630

End: 2733

Strand: Direct

Name: dnaN [H]

Synonym: Csal_0002

Alternate gene names: 92112138

Gene position: 1630-2733 (Clockwise)

Preceding gene: 92112137

Following gene: 92112139

Centisome position: 0.04

GC content: 64.13

Gene sequence:

>1104_bases
ATGAGATTTACCATCACCCGCGAAGCCCTATTGCGCCCGCTATCGCTGGTCGCTGGCGTCGTCGAGCGTCGTCAGACCCT
GCCGGTGCTGTCCAACGTGCTGCTCCAGGTGCAGGATTCGCAGCTGGCATTGACCGGTACGGATCTCGAGGTCGAGTTGA
TCGGCCGCACCACGCCGCATCACGTCGATGAACCGGGCTCTGCCACGGTACCGGCGCGCAAGCTGATGGACATTTGCAAG
TCGCTGCCCGAGCAGACCGACATCACCTTCACGCTCGAGGAAGGACGGGCGATCCTGCGTGCCGGGCGTTCGCGTTTCAC
CTTGTCCACGCTGCCCGTGGCGGAGTTCCCGAATATCGAGGGAAGTCAAAGCGATACCACGCTGACACTGGCGCGTGGCA
CGCTCAAGCATCTGATCGATGCGACGTCCTTCGCCATGGCGCAGCAGGATGTCCGCTACTACCTGAACGGCATGCTGCTC
GAGTTCGGCCACCATCTGGTGCGTACGGTCGCCACCGACGGCCACCGCCTGGCGGTCTGCGCGCGCAGCGCGGACGTCGA
GGTCGAGCCGTCGCAAAAGCTGATCGTGCCTCGCAAGGGGATTCTCGAGCTGGCGCGCCTGCTGGACGGCAGCGACGAGC
CGGTGGAACTCACCATCGGCGGTACGCACCTGCGCGCCCAGACCGGTGATTTCACCTTCACCTCGAAGCTGGTCGACGGC
AAGTTCCCGGATTACGATCGCGTCATTCCGCGTGGGGGCGACAAGGTCGTCATCGCCGAGCGTGGCGAGCTGCGCCAGGT
GCTGTCGCGCACCGCGATTCTCTCCAACGAGAAATATCGCGGAGTGCGACTCAACCTCGAGGAGAACAACCTGCGGGTGA
CGGCGAACAACCCCGAGCAGGAGGAGGCCGAGGAGAACGTGGCCATCGAGTACGATGGGGGCGCGCTGGAAGTCGGTTTC
AACGTGGGGTACCTCGTCGATGTGCTCGGCGTGCTGGACGCCGATCGTGTGCAGATGACGCTTTCCGATCCCAACAGCAG
TGCACTGCTGGAGGAACCGGGCGGCGGCGATGCCATGTACGTGGTCATGCCGATGCGCCTCTGA

Upstream 100 bases:

>100_bases
GCGGATATCCGCGAGGACTACAAGAACCTGCTGCGGCTGCTGACCAGTTGAGCATGGCGGCCCGAATATTCCCTTGGACC
CCAGGACGAGAGTGGAGCCC

Downstream 100 bases:

>100_bases
TCGCCTTGTCCGCCCCTTCACGCGGCTCCGTCCCGGGCGCCTCGTCGGCTCCCTCGCCGATGTCACGCCGGGACGGCGCG
CTCTCTTGATGTCCTCGTAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRFTITREALLRPLSLVAGVVERRQTLPVLSNVLLQVQDSQLALTGTDLEVELIGRTTPHHVDEPGSATVPARKLMDICK
SLPEQTDITFTLEEGRAILRAGRSRFTLSTLPVAEFPNIEGSQSDTTLTLARGTLKHLIDATSFAMAQQDVRYYLNGMLL
EFGHHLVRTVATDGHRLAVCARSADVEVEPSQKLIVPRKGILELARLLDGSDEPVELTIGGTHLRAQTGDFTFTSKLVDG
KFPDYDRVIPRGGDKVVIAERGELRQVLSRTAILSNEKYRGVRLNLEENNLRVTANNPEQEEAEENVAIEYDGGALEVGF
NVGYLVDVLGVLDADRVQMTLSDPNSSALLEEPGGGDAMYVVMPMRL

Sequences:

>Translated_367_residues
MRFTITREALLRPLSLVAGVVERRQTLPVLSNVLLQVQDSQLALTGTDLEVELIGRTTPHHVDEPGSATVPARKLMDICK
SLPEQTDITFTLEEGRAILRAGRSRFTLSTLPVAEFPNIEGSQSDTTLTLARGTLKHLIDATSFAMAQQDVRYYLNGMLL
EFGHHLVRTVATDGHRLAVCARSADVEVEPSQKLIVPRKGILELARLLDGSDEPVELTIGGTHLRAQTGDFTFTSKLVDG
KFPDYDRVIPRGGDKVVIAERGELRQVLSRTAILSNEKYRGVRLNLEENNLRVTANNPEQEEAEENVAIEYDGGALEVGF
NVGYLVDVLGVLDADRVQMTLSDPNSSALLEEPGGGDAMYVVMPMRL
>Mature_367_residues
MRFTITREALLRPLSLVAGVVERRQTLPVLSNVLLQVQDSQLALTGTDLEVELIGRTTPHHVDEPGSATVPARKLMDICK
SLPEQTDITFTLEEGRAILRAGRSRFTLSTLPVAEFPNIEGSQSDTTLTLARGTLKHLIDATSFAMAQQDVRYYLNGMLL
EFGHHLVRTVATDGHRLAVCARSADVEVEPSQKLIVPRKGILELARLLDGSDEPVELTIGGTHLRAQTGDFTFTSKLVDG
KFPDYDRVIPRGGDKVVIAERGELRQVLSRTAILSNEKYRGVRLNLEENNLRVTANNPEQEEAEENVAIEYDGGALEVGF
NVGYLVDVLGVLDADRVQMTLSDPNSSALLEEPGGGDAMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=55.3133514986376, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40296; Mature: 40296

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTITREALLRPLSLVAGVVERRQTLPVLSNVLLQVQDSQLALTGTDLEVELIGRTTPH
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCEEEEEECCCCCC
HVDEPGSATVPARKLMDICKSLPEQTDITFTLEEGRAILRAGRSRFTLSTLPVAEFPNIE
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
GSQSDTTLTLARGTLKHLIDATSFAMAQQDVRYYLNGMLLEFGHHLVRTVATDGHRLAVC
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ARSADVEVEPSQKLIVPRKGILELARLLDGSDEPVELTIGGTHLRAQTGDFTFTSKLVDG
EECCCCEECCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCEEEEEHHHCC
KFPDYDRVIPRGGDKVVIAERGELRQVLSRTAILSNEKYRGVRLNLEENNLRVTANNPEQ
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCH
EEAEENVAIEYDGGALEVGFNVGYLVDVLGVLDADRVQMTLSDPNSSALLEEPGGGDAMY
HHHHCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCEEE
VVMPMRL
EEEECCC
>Mature Secondary Structure
MRFTITREALLRPLSLVAGVVERRQTLPVLSNVLLQVQDSQLALTGTDLEVELIGRTTPH
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCEEEEEECCCCCC
HVDEPGSATVPARKLMDICKSLPEQTDITFTLEEGRAILRAGRSRFTLSTLPVAEFPNIE
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
GSQSDTTLTLARGTLKHLIDATSFAMAQQDVRYYLNGMLLEFGHHLVRTVATDGHRLAVC
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ARSADVEVEPSQKLIVPRKGILELARLLDGSDEPVELTIGGTHLRAQTGDFTFTSKLVDG
EECCCCEECCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCEEEEEHHHCC
KFPDYDRVIPRGGDKVVIAERGELRQVLSRTAILSNEKYRGVRLNLEENNLRVTANNPEQ
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCH
EEAEENVAIEYDGGALEVGFNVGYLVDVLGVLDADRVQMTLSDPNSSALLEEPGGGDAMY
HHHHCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCEEE
VVMPMRL
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]