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The map label for this gene is narI [H]

Identifier: 91790421

GI number: 91790421

Start: 4865355

End: 4866074

Strand: Reverse

Name: narI [H]

Synonym: Bpro_4592

Alternate gene names: 91790421

Gene position: 4866074-4865355 (Counterclockwise)

Preceding gene: 91790422

Following gene: 91790420

Centisome position: 93.57

GC content: 60.0

Gene sequence:

>720_bases
ATGACCACGCTGCACGGTTTTCTTTTTGGTGTCTACCCCTACATCTGCCTGACGGTGTTTCTGCTCGGCAGCCTGATCCG
CTTTGACCGCGACCAGTACACCTGGAAAAGCGATTCCTCGCAGCTGCTTCGCAGCGGCTTGCTGCGCTGGGGCAGCAACC
TGTTCCATGTCGGCATCCTTTTCCTGTTTTTCGGCCATCTGGTGGGTTTGCTGGCACCGCACGCCTTTTACGCGCATTTT
CTCGAAGCGTCAGTCAAGCAGAAGATCGCCATTTACTCGGGCGGTGCGTTCGGCATCCTGTGTTTTATCGGCCTGTCGAT
GCTGATCTATCGCCGCGTCTTTGATCCGCGCATCCGTTACACCAGCCACAAAACCGACCTGGCCATCCTCCTGATTTTGT
GGGTGCAGCTGGTGGTCGGGCTGATTACCCTGCCGTATTCGTGGGCGCATTCGGATGGCAGCGTGATGCTGATCCTGGCC
GACTGGGCGCAGCGCATCCTCACGCTGCGGACGGTGGATTCTGCGGCGCTGGCCGGTCTGCCCTGGCCTTACCTTTTTCA
CATCGTGTTCGGAATGACGATTTTCCTGCTGTTTCCGTTCAGCCGCCTCGTGCATGTCTGGAGCGGGTTCGCGTCGGTGG
GCTATCTGCTTCGTCCCTACCAGGTGGTGCGTAGCCGGCGCCTGGGCGTTCCCGGGGCCAAGTCGCCCGACGGCCGCTAG

Upstream 100 bases:

>100_bases
TGGTTTTTGACGGCTGCTCTTCCAAAGGCCAGGCCCGGCCCGACCAGGCGCAACCGATTCACATTGTGCGCAAGGTGCGT
CCCACCCAAGGAGTACAAGC

Downstream 100 bases:

>100_bases
GTACGCACTGCAAGAAACGAGGCTCCCTCCATGACTACACAAACGCTTTCTTCACCCGCCGCCGCCCAGGGCTGTGGCAG
CGGTTCCTGCCAGTGTGCTT

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTTLHGFLFGVYPYICLTVFLLGSLIRFDRDQYTWKSDSSQLLRSGLLRWGSNLFHVGILFLFFGHLVGLLAPHAFYAHF
LEASVKQKIAIYSGGAFGILCFIGLSMLIYRRVFDPRIRYTSHKTDLAILLILWVQLVVGLITLPYSWAHSDGSVMLILA
DWAQRILTLRTVDSAALAGLPWPYLFHIVFGMTIFLLFPFSRLVHVWSGFASVGYLLRPYQVVRSRRLGVPGAKSPDGR

Sequences:

>Translated_239_residues
MTTLHGFLFGVYPYICLTVFLLGSLIRFDRDQYTWKSDSSQLLRSGLLRWGSNLFHVGILFLFFGHLVGLLAPHAFYAHF
LEASVKQKIAIYSGGAFGILCFIGLSMLIYRRVFDPRIRYTSHKTDLAILLILWVQLVVGLITLPYSWAHSDGSVMLILA
DWAQRILTLRTVDSAALAGLPWPYLFHIVFGMTIFLLFPFSRLVHVWSGFASVGYLLRPYQVVRSRRLGVPGAKSPDGR
>Mature_238_residues
TTLHGFLFGVYPYICLTVFLLGSLIRFDRDQYTWKSDSSQLLRSGLLRWGSNLFHVGILFLFFGHLVGLLAPHAFYAHFL
EASVKQKIAIYSGGAFGILCFIGLSMLIYRRVFDPRIRYTSHKTDLAILLILWVQLVVGLITLPYSWAHSDGSVMLILAD
WAQRILTLRTVDSAALAGLPWPYLFHIVFGMTIFLLFPFSRLVHVWSGFASVGYLLRPYQVVRSRRLGVPGAKSPDGR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=226, Percent_Identity=48.6725663716814, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI1787738, Length=225, Percent_Identity=51.1111111111111, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 27088; Mature: 26957

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLHGFLFGVYPYICLTVFLLGSLIRFDRDQYTWKSDSSQLLRSGLLRWGSNLFHVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
FLFFGHLVGLLAPHAFYAHFLEASVKQKIAIYSGGAFGILCFIGLSMLIYRRVFDPRIRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHC
TSHKTDLAILLILWVQLVVGLITLPYSWAHSDGSVMLILADWAQRILTLRTVDSAALAGL
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
PWPYLFHIVFGMTIFLLFPFSRLVHVWSGFASVGYLLRPYQVVRSRRLGVPGAKSPDGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTLHGFLFGVYPYICLTVFLLGSLIRFDRDQYTWKSDSSQLLRSGLLRWGSNLFHVGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
FLFFGHLVGLLAPHAFYAHFLEASVKQKIAIYSGGAFGILCFIGLSMLIYRRVFDPRIRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHC
TSHKTDLAILLILWVQLVVGLITLPYSWAHSDGSVMLILADWAQRILTLRTVDSAALAGL
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
PWPYLFHIVFGMTIFLLFPFSRLVHVWSGFASVGYLLRPYQVVRSRRLGVPGAKSPDGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]