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The map label for this gene is gsiB [H]

Identifier: 91790239

GI number: 91790239

Start: 4649496

End: 4651121

Strand: Reverse

Name: gsiB [H]

Synonym: Bpro_4406

Alternate gene names: 91790239

Gene position: 4651121-4649496 (Counterclockwise)

Preceding gene: 91790240

Following gene: 91790238

Centisome position: 89.44

GC content: 58.43

Gene sequence:

>1626_bases
ATGGATCGTCGTGATTTCGTCAAAGGCGCAGCAGCTCTTACTGCAACCGCCGCCGCTGGCATCGGGCAAGCGCAACAAGC
CGGGGCGCCAGTTGCGAAGCGCTCAATTCTGACAGCTACGGCGCAGTTCGACCCGGCCCGCCCGGAGATTGCCCGGGTTG
TCGCACAGTCCTGCAAGTCGCTCGGGTGGGAGGTTGAAGCCAATCCCATCGACTACAACCAGGGCATCACGAAAGTGATC
AACGAGCATGACTTCGAGATGTTCCTGGTCTTTCTTCCGGGTACGGCCATCCGCATCGACCCCGACTTCTTCATTCGCAG
CATTCACCACTCCACAGAGCATAAGCGCGGCGGCTTCAACTGGGCGGGCTACAAGAACGACCGTGTAGACGCGCTGGCAA
CCGTGCAGAGCCGCTTGATGAAGATCGACGACCGTAGGAAGCCGGTGCTCGAAGCGCAGGAAACCATCTTCCAGGACCAG
CCTGGAACCGTGCTGGCCTATACGCAGATGACGATGGCTCATCGTTCGGACAAGCTCAAGGGCCTGGTGCCGCAGCTCGG
CGAGGGAATCGGCGGCTTTTGGTCTGACGTCAACATGGAAGTGGCGGGCGACGGATTCTCGCGCACCGGTTCGAATGCGG
ACGTCAAGCATCTGAATCCAGTTGCGGTCAATGACTCCATGGAATTCATGGAGCTTTCCATGATCTACGACCGACTTTTC
CGCCTTGGGCCCGACGGCAAACCTGTACCTTGGGCAGCCTCCGGAATGAAGTTGGTGGATGACAAAACCATCGATCTGAC
GATCCGCTCGGGTATGCGCTGGCATGACGGTAAGCCAGTGACGGCCGAGGATGTCAAGTTCACCTTCGACTATCACAAGA
AGTGGAAGGCGCCATTTTTCCTTGCCGCGCTCAATAATGTCGTCGCGGTGGAGCTCCCGAGCCCCAATACGGTGCGCATC
CGACTCGAAAATCCATCGGCTCCCTTTGTCTCCAACGTGATGGCGACGATGTTTCTTCTCCCCAAACACATTTGGGAAGG
CATCCCGGAGAAGGTCGGCATTGACGATCCGCTCAAATTCCCCAACGACAAGCCGGTCGGTAGCGGACCGTTCAAGTTCG
ACTACTGGCGGCGCGGTTCGGAACTCAAGGTCACCGCGTTCCGCGAGCACTTCAACCCACCCAAGTGCGCAGGCATCATC
CGCATTACTTACGGCAGCCATGACGCGTTGGCCGCCGCCATCGAGAAGGGGGAATGCGACCGCTCACGCTACATCTTGTC
GCCAGCCCTTGTCGATCGTATAAAGAACGTGAAGAACGTAGTGGCCAAGGGCTATCCCAGCCACGGCCTCTATCACCTTG
CGTACAACAACAAGATCAAGCCTTTCGACGATCCCGCCTTCCGCCAGGCGCTCAACCATGTGATGCCGCGCAAGATGATC
TCGGAGCTGATCCTGCTCGGGTACGCCGATCCCGGCGCATCAATTATTTCGCCCATCAGCACGTTTTGGCACAACCCGGC
TGTCAAGGTTCCGGCTGAAGACGTGAAGAAGGCGCGCGACATTCTTGCGAAGGCCGGTTACGGATGGGACGCGCAAGGCA
AATTGCTCTCTCCCCGCGGCAAATGA

Upstream 100 bases:

>100_bases
CTAGATAACGTTACTCCTTGCAACATGGACATGTGAAGTCTGCATTCATAGACTTGTCACATATCGGGCTTGGTGGCCTG
GGAAACTTAAGGAGACAAGA

Downstream 100 bases:

>100_bases
TCTACGGTGCATTGTGGGCGGATGACGCCCCTACTCGAAACCGCGGCCATCAGGGAGGCTCGCGATGACTGCGTATATCC
TGCGCCGTCTCTTGTCGGCA

Product: twin-arginine translocation pathway signal protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MDRRDFVKGAAALTATAAAGIGQAQQAGAPVAKRSILTATAQFDPARPEIARVVAQSCKSLGWEVEANPIDYNQGITKVI
NEHDFEMFLVFLPGTAIRIDPDFFIRSIHHSTEHKRGGFNWAGYKNDRVDALATVQSRLMKIDDRRKPVLEAQETIFQDQ
PGTVLAYTQMTMAHRSDKLKGLVPQLGEGIGGFWSDVNMEVAGDGFSRTGSNADVKHLNPVAVNDSMEFMELSMIYDRLF
RLGPDGKPVPWAASGMKLVDDKTIDLTIRSGMRWHDGKPVTAEDVKFTFDYHKKWKAPFFLAALNNVVAVELPSPNTVRI
RLENPSAPFVSNVMATMFLLPKHIWEGIPEKVGIDDPLKFPNDKPVGSGPFKFDYWRRGSELKVTAFREHFNPPKCAGII
RITYGSHDALAAAIEKGECDRSRYILSPALVDRIKNVKNVVAKGYPSHGLYHLAYNNKIKPFDDPAFRQALNHVMPRKMI
SELILLGYADPGASIISPISTFWHNPAVKVPAEDVKKARDILAKAGYGWDAQGKLLSPRGK

Sequences:

>Translated_541_residues
MDRRDFVKGAAALTATAAAGIGQAQQAGAPVAKRSILTATAQFDPARPEIARVVAQSCKSLGWEVEANPIDYNQGITKVI
NEHDFEMFLVFLPGTAIRIDPDFFIRSIHHSTEHKRGGFNWAGYKNDRVDALATVQSRLMKIDDRRKPVLEAQETIFQDQ
PGTVLAYTQMTMAHRSDKLKGLVPQLGEGIGGFWSDVNMEVAGDGFSRTGSNADVKHLNPVAVNDSMEFMELSMIYDRLF
RLGPDGKPVPWAASGMKLVDDKTIDLTIRSGMRWHDGKPVTAEDVKFTFDYHKKWKAPFFLAALNNVVAVELPSPNTVRI
RLENPSAPFVSNVMATMFLLPKHIWEGIPEKVGIDDPLKFPNDKPVGSGPFKFDYWRRGSELKVTAFREHFNPPKCAGII
RITYGSHDALAAAIEKGECDRSRYILSPALVDRIKNVKNVVAKGYPSHGLYHLAYNNKIKPFDDPAFRQALNHVMPRKMI
SELILLGYADPGASIISPISTFWHNPAVKVPAEDVKKARDILAKAGYGWDAQGKLLSPRGK
>Mature_541_residues
MDRRDFVKGAAALTATAAAGIGQAQQAGAPVAKRSILTATAQFDPARPEIARVVAQSCKSLGWEVEANPIDYNQGITKVI
NEHDFEMFLVFLPGTAIRIDPDFFIRSIHHSTEHKRGGFNWAGYKNDRVDALATVQSRLMKIDDRRKPVLEAQETIFQDQ
PGTVLAYTQMTMAHRSDKLKGLVPQLGEGIGGFWSDVNMEVAGDGFSRTGSNADVKHLNPVAVNDSMEFMELSMIYDRLF
RLGPDGKPVPWAASGMKLVDDKTIDLTIRSGMRWHDGKPVTAEDVKFTFDYHKKWKAPFFLAALNNVVAVELPSPNTVRI
RLENPSAPFVSNVMATMFLLPKHIWEGIPEKVGIDDPLKFPNDKPVGSGPFKFDYWRRGSELKVTAFREHFNPPKCAGII
RITYGSHDALAAAIEKGECDRSRYILSPALVDRIKNVKNVVAKGYPSHGLYHLAYNNKIKPFDDPAFRQALNHVMPRKMI
SELILLGYADPGASIISPISTFWHNPAVKVPAEDVKKARDILAKAGYGWDAQGKLLSPRGK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=321, Percent_Identity=27.1028037383178, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787052, Length=341, Percent_Identity=26.6862170087977, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787762, Length=297, Percent_Identity=25.5892255892256, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082063, Length=201, Percent_Identity=30.8457711442786, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60047; Mature: 60047

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRDFVKGAAALTATAAAGIGQAQQAGAPVAKRSILTATAQFDPARPEIARVVAQSCKS
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
LGWEVEANPIDYNQGITKVINEHDFEMFLVFLPGTAIRIDPDFFIRSIHHSTEHKRGGFN
CCCEEECCCCCCCCCHHHHHCCCCEEEEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCC
WAGYKNDRVDALATVQSRLMKIDDRRKPVLEAQETIFQDQPGTVLAYTQMTMAHRSDKLK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
GLVPQLGEGIGGFWSDVNMEVAGDGFSRTGSNADVKHLNPVAVNDSMEFMELSMIYDRLF
HHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
RLGPDGKPVPWAASGMKLVDDKTIDLTIRSGMRWHDGKPVTAEDVKFTFDYHKKWKAPFF
HCCCCCCCCCCCCCCCEEECCCEEEEEEECCCEECCCCCCCHHCEEEEEEHHHCCCCCEE
LAALNNVVAVELPSPNTVRIRLENPSAPFVSNVMATMFLLPKHIWEGIPEKVGIDDPLKF
EEECCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PNDKPVGSGPFKFDYWRRGSELKVTAFREHFNPPKCAGIIRITYGSHDALAAAIEKGECD
CCCCCCCCCCCEEHHHCCCCCEEEEEHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCC
RSRYILSPALVDRIKNVKNVVAKGYPSHGLYHLAYNNKIKPFDDPAFRQALNHVMPRKMI
CCCEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
SELILLGYADPGASIISPISTFWHNPAVKVPAEDVKKARDILAKAGYGWDAQGKLLSPRG
HHHHHEECCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
K
C
>Mature Secondary Structure
MDRRDFVKGAAALTATAAAGIGQAQQAGAPVAKRSILTATAQFDPARPEIARVVAQSCKS
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
LGWEVEANPIDYNQGITKVINEHDFEMFLVFLPGTAIRIDPDFFIRSIHHSTEHKRGGFN
CCCEEECCCCCCCCCHHHHHCCCCEEEEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCC
WAGYKNDRVDALATVQSRLMKIDDRRKPVLEAQETIFQDQPGTVLAYTQMTMAHRSDKLK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
GLVPQLGEGIGGFWSDVNMEVAGDGFSRTGSNADVKHLNPVAVNDSMEFMELSMIYDRLF
HHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
RLGPDGKPVPWAASGMKLVDDKTIDLTIRSGMRWHDGKPVTAEDVKFTFDYHKKWKAPFF
HCCCCCCCCCCCCCCCEEECCCEEEEEEECCCEECCCCCCCHHCEEEEEEHHHCCCCCEE
LAALNNVVAVELPSPNTVRIRLENPSAPFVSNVMATMFLLPKHIWEGIPEKVGIDDPLKF
EEECCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PNDKPVGSGPFKFDYWRRGSELKVTAFREHFNPPKCAGIIRITYGSHDALAAAIEKGECD
CCCCCCCCCCCEEHHHCCCCCEEEEEHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCC
RSRYILSPALVDRIKNVKNVVAKGYPSHGLYHLAYNNKIKPFDDPAFRQALNHVMPRKMI
CCCEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
SELILLGYADPGASIISPISTFWHNPAVKVPAEDVKKARDILAKAGYGWDAQGKLLSPRG
HHHHHEECCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA