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The map label for this gene is gatA2 [H]

Identifier: 91790199

GI number: 91790199

Start: 4605320

End: 4606735

Strand: Direct

Name: gatA2 [H]

Synonym: Bpro_4364

Alternate gene names: 91790199

Gene position: 4605320-4606735 (Clockwise)

Preceding gene: 91790192

Following gene: 91790201

Centisome position: 88.56

GC content: 63.98

Gene sequence:

>1416_bases
ATGGACTTTGCTTTCCTTTCCGCCACCGATCTCGGACTAGCATTGGCCAAGGGCGAGCTCACGTCAGTGGCACTCGTGAC
TGAGTTGCTCGCTCGCATCCGCACCTACGACCCAATATTGAACGCTTTCGTCGAGGTCTATGGCGACGCTGCACTTCTGG
CGGCACGGGACTCGGACGGACGGCGGTCCCGCAACCAGGCAAGGGGCCCCCTTGACGGCGTTCCGTTCGCGGTCAAGGAT
CTGTTCGACGTCGAGGGCCATGCCACGTTGGCCGGATCGAAGGCGTCCGCCCCTGCGGCCGCTGCGCAATCGGCCACCGC
GGTCCGGCGATTGCTAGCTGAGGGCATGGTCTTGCTCGGCAAGACGCATACGGTCGAGTTCGCGTACGGCAGCTGGGGAA
CGAACCCTAGCTGCGGCACCCCGGTCAACCCGCGCGATCCTATAGTTCAGCGCGTCCCGGGCGGATCGTCCAGCGGATCG
GGTGTGGCGGTGGCAGCCGGGCTGGTGCCCGTCGCGCTGGGCACCGATACAGGCGGCTCGGTGCGCAGCCCGTCGGCACT
ATGCGGGATCGTCGGGATGAAGACGTCGCTCGGACTGATCGGGCGCGGTGGGGTGCAGCCGCTCGCCCTTGTCTTCGACG
CCGTGGGCCCAATGACTCGCTCCGTCGAGGACGTGGCGCTCATCCAGGCGGCGTTGCAAGGAGAGGATGCGGACGATCCG
GCGACGTTCGGGGTACCGCGGACGGATTCGCTCGTCGACCTAAACAAGGGTGTCGCAGGGCTGACGCTGCGCTACCCGAC
CATGGAGAACCTAAAGGCTGCGGAACCCGACATCCTTGACCGGTTCAAGGAGACGCTCGCTGATCTGAAAGCCATGGGCG
CCGTCCTCGACGAGACGCCGCTACCTCGTCCCCTCGAAACATATGCGACCCTCACGGCCAATATCACGACGGTGGAGGCC
TGGAGCCGTTACCGACATCTAATCGAGAAGGAGAACAGCCTCGTGGACCCGGAAATCGCGACGCGGATGTCGCGCGGCAG
AACGATGAGTATCACGGATTACCTCGGCTACCTGGAGCAGCGGCGGCTGATGCAAACGGAGTTTCACCGCTACTTTGCCG
GGGCGAATGCCGTCCTGCTTCCCTCGTCGCCCATCACAGCGAAGCCCATAGACGGAGTACACAATACCAACGCTCCGTTT
GGCCTTTTCACTCGTCTCGCCAATCTGATGGACCTCGCCTCCCTGAGCATACCGATGGGTGACGCCGAGGGCCTCCCCAC
CGCTGTTCAGATCATGGTCCGTCGCTACGCCGACCCCCTGGCTTTTCGAATCGGACGCGCGCTTGAAATTCAGCGCGGCG
GATTGTTCGTGCCCCCTCCCGGCTACGAGCGCCTGGATCGCACGAAGATGCTTTGA

Upstream 100 bases:

>100_bases
GAGCTCTTCGCCGATCCGATCATTTTAGTCCGAATACAATCGGTTGCCTATTAAATACAATCGATTGGATTACAGTATGA
ACCTTATAAAAGGAGCTGAC

Downstream 100 bases:

>100_bases
CTTAAAGTATGAGTCATCCATCTGCTGGCCGTTTTCGCCACTCAATGGCCGTATTCGACAACAAGTAGTGCTGTTGCTGC
CGTCTTGCCTTGATTGGCAA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDFAFLSATDLGLALAKGELTSVALVTELLARIRTYDPILNAFVEVYGDAALLAARDSDGRRSRNQARGPLDGVPFAVKD
LFDVEGHATLAGSKASAPAAAAQSATAVRRLLAEGMVLLGKTHTVEFAYGSWGTNPSCGTPVNPRDPIVQRVPGGSSSGS
GVAVAAGLVPVALGTDTGGSVRSPSALCGIVGMKTSLGLIGRGGVQPLALVFDAVGPMTRSVEDVALIQAALQGEDADDP
ATFGVPRTDSLVDLNKGVAGLTLRYPTMENLKAAEPDILDRFKETLADLKAMGAVLDETPLPRPLETYATLTANITTVEA
WSRYRHLIEKENSLVDPEIATRMSRGRTMSITDYLGYLEQRRLMQTEFHRYFAGANAVLLPSSPITAKPIDGVHNTNAPF
GLFTRLANLMDLASLSIPMGDAEGLPTAVQIMVRRYADPLAFRIGRALEIQRGGLFVPPPGYERLDRTKML

Sequences:

>Translated_471_residues
MDFAFLSATDLGLALAKGELTSVALVTELLARIRTYDPILNAFVEVYGDAALLAARDSDGRRSRNQARGPLDGVPFAVKD
LFDVEGHATLAGSKASAPAAAAQSATAVRRLLAEGMVLLGKTHTVEFAYGSWGTNPSCGTPVNPRDPIVQRVPGGSSSGS
GVAVAAGLVPVALGTDTGGSVRSPSALCGIVGMKTSLGLIGRGGVQPLALVFDAVGPMTRSVEDVALIQAALQGEDADDP
ATFGVPRTDSLVDLNKGVAGLTLRYPTMENLKAAEPDILDRFKETLADLKAMGAVLDETPLPRPLETYATLTANITTVEA
WSRYRHLIEKENSLVDPEIATRMSRGRTMSITDYLGYLEQRRLMQTEFHRYFAGANAVLLPSSPITAKPIDGVHNTNAPF
GLFTRLANLMDLASLSIPMGDAEGLPTAVQIMVRRYADPLAFRIGRALEIQRGGLFVPPPGYERLDRTKML
>Mature_471_residues
MDFAFLSATDLGLALAKGELTSVALVTELLARIRTYDPILNAFVEVYGDAALLAARDSDGRRSRNQARGPLDGVPFAVKD
LFDVEGHATLAGSKASAPAAAAQSATAVRRLLAEGMVLLGKTHTVEFAYGSWGTNPSCGTPVNPRDPIVQRVPGGSSSGS
GVAVAAGLVPVALGTDTGGSVRSPSALCGIVGMKTSLGLIGRGGVQPLALVFDAVGPMTRSVEDVALIQAALQGEDADDP
ATFGVPRTDSLVDLNKGVAGLTLRYPTMENLKAAEPDILDRFKETLADLKAMGAVLDETPLPRPLETYATLTANITTVEA
WSRYRHLIEKENSLVDPEIATRMSRGRTMSITDYLGYLEQRRLMQTEFHRYFAGANAVLLPSSPITAKPIDGVHNTNAPF
GLFTRLANLMDLASLSIPMGDAEGLPTAVQIMVRRYADPLAFRIGRALEIQRGGLFVPPPGYERLDRTKML

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=29.2, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=24.8484848484848, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=425, Percent_Identity=26.8235294117647, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=26.2626262626263, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=234, Percent_Identity=30.3418803418803, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=213, Percent_Identity=29.1079812206573, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=412, Percent_Identity=27.1844660194175, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=197, Percent_Identity=32.994923857868, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=28.82096069869, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=25.7367387033399, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=24.6987951807229, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=24.6987951807229, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=24.6987951807229, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=24.6987951807229, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=26.6533066132265, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=25.9557344064386, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=421, Percent_Identity=25.1781472684086, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648439, Length=421, Percent_Identity=25.1781472684086, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50015; Mature: 50015

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFAFLSATDLGLALAKGELTSVALVTELLARIRTYDPILNAFVEVYGDAALLAARDSDG
CCCEEEEHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
RRSRNQARGPLDGVPFAVKDLFDVEGHATLAGSKASAPAAAAQSATAVRRLLAEGMVLLG
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEC
KTHTVEFAYGSWGTNPSCGTPVNPRDPIVQRVPGGSSSGSGVAVAAGLVPVALGTDTGGS
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCEEEEECCCCCCC
VRSPSALCGIVGMKTSLGLIGRGGVQPLALVFDAVGPMTRSVEDVALIQAALQGEDADDP
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
ATFGVPRTDSLVDLNKGVAGLTLRYPTMENLKAAEPDILDRFKETLADLKAMGAVLDETP
CCCCCCCCCCHHHHCCCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LPRPLETYATLTANITTVEAWSRYRHLIEKENSLVDPEIATRMSRGRTMSITDYLGYLEQ
CCCCHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
RRLMQTEFHRYFAGANAVLLPSSPITAKPIDGVHNTNAPFGLFTRLANLMDLASLSIPMG
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DAEGLPTAVQIMVRRYADPLAFRIGRALEIQRGGLFVPPPGYERLDRTKML
CCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MDFAFLSATDLGLALAKGELTSVALVTELLARIRTYDPILNAFVEVYGDAALLAARDSDG
CCCEEEEHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
RRSRNQARGPLDGVPFAVKDLFDVEGHATLAGSKASAPAAAAQSATAVRRLLAEGMVLLG
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEC
KTHTVEFAYGSWGTNPSCGTPVNPRDPIVQRVPGGSSSGSGVAVAAGLVPVALGTDTGGS
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCEEEEECCCCCCC
VRSPSALCGIVGMKTSLGLIGRGGVQPLALVFDAVGPMTRSVEDVALIQAALQGEDADDP
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
ATFGVPRTDSLVDLNKGVAGLTLRYPTMENLKAAEPDILDRFKETLADLKAMGAVLDETP
CCCCCCCCCCHHHHCCCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LPRPLETYATLTANITTVEAWSRYRHLIEKENSLVDPEIATRMSRGRTMSITDYLGYLEQ
CCCCHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
RRLMQTEFHRYFAGANAVLLPSSPITAKPIDGVHNTNAPFGLFTRLANLMDLASLSIPMG
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DAEGLPTAVQIMVRRYADPLAFRIGRALEIQRGGLFVPPPGYERLDRTKML
CCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]