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The map label for this gene is gcvA [H]

Identifier: 91790192

GI number: 91790192

Start: 4595684

End: 4596595

Strand: Direct

Name: gcvA [H]

Synonym: Bpro_4357

Alternate gene names: 91790192

Gene position: 4595684-4596595 (Clockwise)

Preceding gene: 91790183

Following gene: 91790199

Centisome position: 88.37

GC content: 55.04

Gene sequence:

>912_bases
GTGGACATGAAACGAGCATGTCCCACAATCCAAGAATTGCTTGCTTTTGACGCTGTCGCTCGGCACGGCAGCCTGACCCG
TGCCGCAGGCGCGCTGTGCGTTTCGGTTAGCGGCATCAGCAAACAGCTTGCCGGATTGGAAAGTTTTGTCGGACATCCGC
TGCTCGAGAAACAGGGGCGTGGCGTCCAGTTGACCACCATTGGTCGTGAGTACTGGACGAAAATTGGCCCGAGCTTGCGG
ACAATTGAGACGGCGAGCTTTGAGGCGAAGTCAGCAGGGTCCGGCGCTGGAGTGCTTTCGTTGGCGAGCGTGCCCACGTT
TTTGACTAAGTGGCTGATTCCGCGATTGGCTGACTTTCGTCGGCTTCATCCGGGTGTGACGTTCAGCTTCAGCCAGCATC
TGGGTTCTACAGAGCCCCATCCTACTGAGATTGATGCTGCAATTCGCTACGGCTCAGCTGTGTGGCCGGGCGTAGAGAGC
GAATACATTGCTGGTCGCGAGTTTGTCTGCGTATGCTCCACTGAGCATCTCCAAAGCAGGAGATGGACACCGAAAGAAGT
GCTCAAACAAACGTTGCTACATCACGACCAAGCCCCTGCAGCATGGGCTCACTGGGCGGAGCGGCACGGTATCGACGACG
TTCACACGCTCGCAGGTCCGAGATTTGCTCAATATTCGGCGCTAATACAAGCTGTTCTAAGCGGGCTTGGAATAGGCCTT
GTCCCAAAAATCCTGATTGAAGAAGAACTCGCCGAGAAACGAGCGATTGCCCTGGGAGAAGTTGTCGTTATGGATCAGGG
GCACTATCTGTGCTTTATGCCAAATCGCCTGGATCGCCCGATTTTTGCGGCGTTTCGCACTTGGATACTGCAAGAGGGTA
AAGGCAGCTTATCAAGTGATACAGCCCCCTAA

Upstream 100 bases:

>100_bases
GAAGTCTTCCAGCAATGCTCGAATTCTAGGCTTCACCTCGCAGGCGACAAGTGAAAAATCTGCCTCAACTGTTGACGATT
AGGAAAGTGTTTGTCATAGT

Downstream 100 bases:

>100_bases
AAAGGGCGACCATGCTGTTGCTGTTGGGGTCTACTGACAGGGCTCTCGGCCTTACCTCTTCCCTTCGTGTACGAATTAAC
TAATAGCCAGAGTTGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDMKRACPTIQELLAFDAVARHGSLTRAAGALCVSVSGISKQLAGLESFVGHPLLEKQGRGVQLTTIGREYWTKIGPSLR
TIETASFEAKSAGSGAGVLSLASVPTFLTKWLIPRLADFRRLHPGVTFSFSQHLGSTEPHPTEIDAAIRYGSAVWPGVES
EYIAGREFVCVCSTEHLQSRRWTPKEVLKQTLLHHDQAPAAWAHWAERHGIDDVHTLAGPRFAQYSALIQAVLSGLGIGL
VPKILIEEELAEKRAIALGEVVVMDQGHYLCFMPNRLDRPIFAAFRTWILQEGKGSLSSDTAP

Sequences:

>Translated_303_residues
MDMKRACPTIQELLAFDAVARHGSLTRAAGALCVSVSGISKQLAGLESFVGHPLLEKQGRGVQLTTIGREYWTKIGPSLR
TIETASFEAKSAGSGAGVLSLASVPTFLTKWLIPRLADFRRLHPGVTFSFSQHLGSTEPHPTEIDAAIRYGSAVWPGVES
EYIAGREFVCVCSTEHLQSRRWTPKEVLKQTLLHHDQAPAAWAHWAERHGIDDVHTLAGPRFAQYSALIQAVLSGLGIGL
VPKILIEEELAEKRAIALGEVVVMDQGHYLCFMPNRLDRPIFAAFRTWILQEGKGSLSSDTAP
>Mature_303_residues
MDMKRACPTIQELLAFDAVARHGSLTRAAGALCVSVSGISKQLAGLESFVGHPLLEKQGRGVQLTTIGREYWTKIGPSLR
TIETASFEAKSAGSGAGVLSLASVPTFLTKWLIPRLADFRRLHPGVTFSFSQHLGSTEPHPTEIDAAIRYGSAVWPGVES
EYIAGREFVCVCSTEHLQSRRWTPKEVLKQTLLHHDQAPAAWAHWAERHGIDDVHTLAGPRFAQYSALIQAVLSGLGIGL
VPKILIEEELAEKRAIALGEVVVMDQGHYLCFMPNRLDRPIFAAFRTWILQEGKGSLSSDTAP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=30.2405498281787, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=26.2626262626263, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=27.3684210526316, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=233, Percent_Identity=30.4721030042918, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=264, Percent_Identity=26.5151515151515, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=25.1968503937008, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=24.9158249158249, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33099; Mature: 33099

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMKRACPTIQELLAFDAVARHGSLTRAAGALCVSVSGISKQLAGLESFVGHPLLEKQGR
CCCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHCCC
GVQLTTIGREYWTKIGPSLRTIETASFEAKSAGSGAGVLSLASVPTFLTKWLIPRLADFR
CEEEEECCHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLHPGVTFSFSQHLGSTEPHPTEIDAAIRYGSAVWPGVESEYIAGREFVCVCSTEHLQSR
HHCCCCEEEHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCEEEEECHHHHHHC
RWTPKEVLKQTLLHHDQAPAAWAHWAERHGIDDVHTLAGPRFAQYSALIQAVLSGLGIGL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHH
VPKILIEEELAEKRAIALGEVVVMDQGHYLCFMPNRLDRPIFAAFRTWILQEGKGSLSSD
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
TAP
CCC
>Mature Secondary Structure
MDMKRACPTIQELLAFDAVARHGSLTRAAGALCVSVSGISKQLAGLESFVGHPLLEKQGR
CCCHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHCCC
GVQLTTIGREYWTKIGPSLRTIETASFEAKSAGSGAGVLSLASVPTFLTKWLIPRLADFR
CEEEEECCHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLHPGVTFSFSQHLGSTEPHPTEIDAAIRYGSAVWPGVESEYIAGREFVCVCSTEHLQSR
HHCCCCEEEHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCEEEEECHHHHHHC
RWTPKEVLKQTLLHHDQAPAAWAHWAERHGIDDVHTLAGPRFAQYSALIQAVLSGLGIGL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHH
VPKILIEEELAEKRAIALGEVVVMDQGHYLCFMPNRLDRPIFAAFRTWILQEGKGSLSSD
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
TAP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]