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The map label for this gene is potH [H]

Identifier: 91790194

GI number: 91790194

Start: 4598283

End: 4600058

Strand: Reverse

Name: potH [H]

Synonym: Bpro_4359

Alternate gene names: 91790194

Gene position: 4600058-4598283 (Counterclockwise)

Preceding gene: 91790195

Following gene: 91790193

Centisome position: 88.46

GC content: 58.67

Gene sequence:

>1776_bases
ATGACACGCAGCAGTAGGTTCGTAAATTGGGTTGTAAATTGGGCGCGGTTCTTACTGCTGACTCCAATAGCCATCTTGCT
TCTCCTCGCCTTCGCTCTACCGTTGCTGGCGATCGTGCCGGCGGCGTTCGGCGGTGATGCCGCCGGGCGTTTCATACGAA
TTTTCGGCAATCCCGTGTACCTAACGGTGATCTGGACAACGGTGCGCATTAGCCTGGAAGCCACAGCGATAACTCTCGTT
GTCTCGTTCCCGCTGGCATGGATCTTGAGCCGGGCGACTGGGGTGAAGGCGCTGCTTCTTGGGCTGTTGGTGCTGGTTCC
CTTCCTGACCAGCGTGATGGTGCGGACGTTTGCTTGGCTGGCGATCCTCGGACAACGTGGCCTTGTCAACGCGACGCTTC
TCAGCCTGGGGTTGACCTCAGAGCCGCTCTCGCTTCTGTTCAGCGAACTGGCAGTTGTGCTCGCCCTCGTGCAATGTTCG
ATCCCGATGATGGTCTTCGCTCTCGTCACCGTCCTCCGGCGCATTGACGGACGCATTCTCCTGGCCGCTCACACGCTCGG
GGCCAGCCCGCTGCGCATCTGGTCAGGCGTACTGATTCCTCTCGCAATCCGCGGCATCCAATCCGGCGTTACCATCATCT
TCCTCTTCACCATGGCGAGCTTCATCGCGCCGACCTTTCTCGGCAGTCAGCGCCAGCAGATGCTCGCCCAAGTAGTTCAG
TCGGAAATCGACATCGGAGCCGACTGGGCTTTGGCCGCGGCACTCGGCATCACGCTTGCCCTCATAGCGACGATCATCGT
CACGGCACTCGCCGCGGTAGCGAGATACTTGTCGCGTTGGCAGCGTCCGGGTTTATTGCCGCTGACTATGCCGCAAGAGC
ATGTGGAGCAGGTGGACAAGTCGCTTCTTCGCAGACCGGTAAAGCTGCTTCCCTCCCGTCTCCACATTCCTGCGGGTTTT
CGCGGCGGCGCGCGATTTGTCGAACCGGTCTATCTGTTGTTCATATCTGCCTATATCCTCCTTCCGCTGGTCGTGCTCAT
TCCTGTGTCGTTCAGCTCGGCAGAGGTTCTCATCTTTCCGCCACCCGGCTACTCCTTGCGATGGTTCGAAACGATCTTTC
ACAGCCAGGAATGGATTGCGGCGGCACTGACCAGCCTTCGGATCGGCGCCGCCGTCTGCGTCGCGACCCTCGTCATCGCC
ACCCTGACGGTTCTTGGCATTGGTCGCCATTTCCGTTACCAGGGTGCTGCCGAAGCGCTGCTGATGTCGCCCTTGACCGT
CCCAAGCGTTGTTTTCGCGCTAGGTGCTTATCTGGCCTTCTCGCGTGTGGGACTCGTGGATACCGAGACAGGGATCATCA
TTGCTCATACTGTTTTGATATTCCCAATTGTTTACCAGGTCGCGTCGGCAACATTTAGTTCCATCGACCCTTGGCTGTCC
CTTGCAGCGGCAAGCCTTGGGGCGAATCCTTGGCGCATCTTTCGAACGATCGTTTTCCCCTTGCTCCTTCCGGGCCTCGC
AATCGGAGCTCTGCTTGCGATGCTTCTCTCGTTCGATGAATCTGTCTCTTCGATCTTTCTCAGCAACCTGTCGGTGAAGA
CCTTGCCGAGAAAGTTGTGGGAAGGCATTCGCTTCAATACCAGTCCCGAATCTGCGGCCGTCTCCGTCGTGCTGCTGGGT
GTGACATGCGCCGTGGTCGTTGTCGGCATGGTGGTTGTACTCAGGCGCCGGAAGAACGCTCTGTTGCCGGTGCAAGCGCG
CCCTGAACCGGTTTGA

Upstream 100 bases:

>100_bases
TTCTTAACTCGAAATGGTGGAACGAGAACCTCGAGTCTGTGACGGCCCGCTGGAACACGTACGTCTTGCGCTAACAGCGC
GTAAATAGGAGAACGCGAAG

Downstream 100 bases:

>100_bases
CGGCATCAGCTGTCTTCGTTGGCGCACCCGTGAAGAACACTGGTCCTCGATTCAGACAGGTCCATGGCTGAGCAAGATGG
GTCGTTGCCGATTACCGATT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MTRSSRFVNWVVNWARFLLLTPIAILLLLAFALPLLAIVPAAFGGDAAGRFIRIFGNPVYLTVIWTTVRISLEATAITLV
VSFPLAWILSRATGVKALLLGLLVLVPFLTSVMVRTFAWLAILGQRGLVNATLLSLGLTSEPLSLLFSELAVVLALVQCS
IPMMVFALVTVLRRIDGRILLAAHTLGASPLRIWSGVLIPLAIRGIQSGVTIIFLFTMASFIAPTFLGSQRQQMLAQVVQ
SEIDIGADWALAAALGITLALIATIIVTALAAVARYLSRWQRPGLLPLTMPQEHVEQVDKSLLRRPVKLLPSRLHIPAGF
RGGARFVEPVYLLFISAYILLPLVVLIPVSFSSAEVLIFPPPGYSLRWFETIFHSQEWIAAALTSLRIGAAVCVATLVIA
TLTVLGIGRHFRYQGAAEALLMSPLTVPSVVFALGAYLAFSRVGLVDTETGIIIAHTVLIFPIVYQVASATFSSIDPWLS
LAAASLGANPWRIFRTIVFPLLLPGLAIGALLAMLLSFDESVSSIFLSNLSVKTLPRKLWEGIRFNTSPESAAVSVVLLG
VTCAVVVVGMVVVLRRRKNALLPVQARPEPV

Sequences:

>Translated_591_residues
MTRSSRFVNWVVNWARFLLLTPIAILLLLAFALPLLAIVPAAFGGDAAGRFIRIFGNPVYLTVIWTTVRISLEATAITLV
VSFPLAWILSRATGVKALLLGLLVLVPFLTSVMVRTFAWLAILGQRGLVNATLLSLGLTSEPLSLLFSELAVVLALVQCS
IPMMVFALVTVLRRIDGRILLAAHTLGASPLRIWSGVLIPLAIRGIQSGVTIIFLFTMASFIAPTFLGSQRQQMLAQVVQ
SEIDIGADWALAAALGITLALIATIIVTALAAVARYLSRWQRPGLLPLTMPQEHVEQVDKSLLRRPVKLLPSRLHIPAGF
RGGARFVEPVYLLFISAYILLPLVVLIPVSFSSAEVLIFPPPGYSLRWFETIFHSQEWIAAALTSLRIGAAVCVATLVIA
TLTVLGIGRHFRYQGAAEALLMSPLTVPSVVFALGAYLAFSRVGLVDTETGIIIAHTVLIFPIVYQVASATFSSIDPWLS
LAAASLGANPWRIFRTIVFPLLLPGLAIGALLAMLLSFDESVSSIFLSNLSVKTLPRKLWEGIRFNTSPESAAVSVVLLG
VTCAVVVVGMVVVLRRRKNALLPVQARPEPV
>Mature_590_residues
TRSSRFVNWVVNWARFLLLTPIAILLLLAFALPLLAIVPAAFGGDAAGRFIRIFGNPVYLTVIWTTVRISLEATAITLVV
SFPLAWILSRATGVKALLLGLLVLVPFLTSVMVRTFAWLAILGQRGLVNATLLSLGLTSEPLSLLFSELAVVLALVQCSI
PMMVFALVTVLRRIDGRILLAAHTLGASPLRIWSGVLIPLAIRGIQSGVTIIFLFTMASFIAPTFLGSQRQQMLAQVVQS
EIDIGADWALAAALGITLALIATIIVTALAAVARYLSRWQRPGLLPLTMPQEHVEQVDKSLLRRPVKLLPSRLHIPAGFR
GGARFVEPVYLLFISAYILLPLVVLIPVSFSSAEVLIFPPPGYSLRWFETIFHSQEWIAAALTSLRIGAAVCVATLVIAT
LTVLGIGRHFRYQGAAEALLMSPLTVPSVVFALGAYLAFSRVGLVDTETGIIIAHTVLIFPIVYQVASATFSSIDPWLSL
AAASLGANPWRIFRTIVFPLLLPGLAIGALLAMLLSFDESVSSIFLSNLSVKTLPRKLWEGIRFNTSPESAAVSVVLLGV
TCAVVVVGMVVVLRRRKNALLPVQARPEPV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=214, Percent_Identity=32.7102803738318, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787369, Length=272, Percent_Identity=29.0441176470588, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787368, Length=217, Percent_Identity=24.8847926267281, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787713, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=26.8939393939394, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787714, Length=205, Percent_Identity=30.2439024390244, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 63833; Mature: 63701

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSSRFVNWVVNWARFLLLTPIAILLLLAFALPLLAIVPAAFGGDAAGRFIRIFGNPVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCEE
LTVIWTTVRISLEATAITLVVSFPLAWILSRATGVKALLLGLLVLVPFLTSVMVRTFAWL
EEEEEEHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AILGQRGLVNATLLSLGLTSEPLSLLFSELAVVLALVQCSIPMMVFALVTVLRRIDGRIL
HHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LAAHTLGASPLRIWSGVLIPLAIRGIQSGVTIIFLFTMASFIAPTFLGSQRQQMLAQVVQ
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SEIDIGADWALAAALGITLALIATIIVTALAAVARYLSRWQRPGLLPLTMPQEHVEQVDK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SLLRRPVKLLPSRLHIPAGFRGGARFVEPVYLLFISAYILLPLVVLIPVSFSSAEVLIFP
HHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PPGYSLRWFETIFHSQEWIAAALTSLRIGAAVCVATLVIATLTVLGIGRHFRYQGAAEAL
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHH
LMSPLTVPSVVFALGAYLAFSRVGLVDTETGIIIAHTVLIFPIVYQVASATFSSIDPWLS
HHCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LAAASLGANPWRIFRTIVFPLLLPGLAIGALLAMLLSFDESVSSIFLSNLSVKTLPRKLW
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGIRFNTSPESAAVSVVLLGVTCAVVVVGMVVVLRRRKNALLPVQARPEPV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRSSRFVNWVVNWARFLLLTPIAILLLLAFALPLLAIVPAAFGGDAAGRFIRIFGNPVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCEE
LTVIWTTVRISLEATAITLVVSFPLAWILSRATGVKALLLGLLVLVPFLTSVMVRTFAWL
EEEEEEHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AILGQRGLVNATLLSLGLTSEPLSLLFSELAVVLALVQCSIPMMVFALVTVLRRIDGRIL
HHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LAAHTLGASPLRIWSGVLIPLAIRGIQSGVTIIFLFTMASFIAPTFLGSQRQQMLAQVVQ
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SEIDIGADWALAAALGITLALIATIIVTALAAVARYLSRWQRPGLLPLTMPQEHVEQVDK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SLLRRPVKLLPSRLHIPAGFRGGARFVEPVYLLFISAYILLPLVVLIPVSFSSAEVLIFP
HHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PPGYSLRWFETIFHSQEWIAAALTSLRIGAAVCVATLVIATLTVLGIGRHFRYQGAAEAL
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHH
LMSPLTVPSVVFALGAYLAFSRVGLVDTETGIIIAHTVLIFPIVYQVASATFSSIDPWLS
HHCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LAAASLGANPWRIFRTIVFPLLLPGLAIGALLAMLLSFDESVSSIFLSNLSVKTLPRKLW
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGIRFNTSPESAAVSVVLLGVTCAVVVVGMVVVLRRRKNALLPVQARPEPV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]