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The map label for this gene is paaF [C]

Identifier: 91789997

GI number: 91789997

Start: 4374335

End: 4375114

Strand: Reverse

Name: paaF [C]

Synonym: Bpro_4159

Alternate gene names: 91789997

Gene position: 4375114-4374335 (Counterclockwise)

Preceding gene: 91789998

Following gene: 91789996

Centisome position: 84.13

GC content: 63.59

Gene sequence:

>780_bases
ATGGCTGCAATTCAAATTACCCAAGCGGGGCCGCTGCTGACCCTCACCCTGAGTCGTCCGGAGGTGCGCAATGCTTTCAA
CGATGAAGTGATCGCCGAATTGACAGCGGCGTTCGCGCAGGCAGGCCGGCGCGAGGACGTGCGCGCGGTGGTGCTGGCGG
CCGAAGGGCCGGCCTTTTGCGCTGGCGCCGACCTGAACTGGATGCGGCGCATGGCGGACTACACGCGCGAGGAAAACCTG
GCCGATGCGGCGCAACTGGCCGAGATGCTGCGTGCCATTTATGAATGCCCGAAACCCACCGTGGCGCGCATTCAGGGCGA
TGTGTATGCGGGCGGCATGGGGCTGGTGGCGGCCTGCGACATCGCCGTCTCTGGCGACACGGCGCAGTACTGCCTCAGCG
AAGTCAAGCTGGGCCTGATCCCAGCCACCATCAGCCCTTATGTGATTCGCGCCATGGGCGCGCGCGCAGCCCACCGCTAT
TTTTTGACGGCTGAGCGTTTTGATGCCCAGGAGGCGCATCGCATCGGTTTCATTCATGAAGTGGTGAGCGCCGTCGCGCT
CGATGCCAAGGTGGCCGAGATTGCACAGGCGCTGGTCAATGCCAGCCCGCAGGCGGTGAAAGCCTGCAAGCGGCTGGTGC
AGGATGTGGCACAGCACGACATCACGCCCGCCCTGATCGCGCAAACCGTGCAGGGCATTGCCGATATTCGTTCCAGCGAA
CAGGGCAAGGAGGGCGTGCAGTCTTTCCTGCAAAAGCGCAAGCCGTCCTGGATCATCTGA

Upstream 100 bases:

>100_bases
CGACCCGGCCGATACGCGCCGTGTGCTGGCGCTGGGCTTGAGCGCTGCGCGCAATGCGCCGATTCCAGAGACCAAGTTTG
GTTTGTTCAGGATGTAAGCC

Downstream 100 bases:

>100_bases
TGCTCCCCCCCTTTGCTTGCTCACTTCGTGTAGCCGCCTCCCCCCTCAAGGGGGTAACACCAGCGGTCCGGCCCTTCGAC
AGGCTCAGGACAGGCCAAGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MAAIQITQAGPLLTLTLSRPEVRNAFNDEVIAELTAAFAQAGRREDVRAVVLAAEGPAFCAGADLNWMRRMADYTREENL
ADAAQLAEMLRAIYECPKPTVARIQGDVYAGGMGLVAACDIAVSGDTAQYCLSEVKLGLIPATISPYVIRAMGARAAHRY
FLTAERFDAQEAHRIGFIHEVVSAVALDAKVAEIAQALVNASPQAVKACKRLVQDVAQHDITPALIAQTVQGIADIRSSE
QGKEGVQSFLQKRKPSWII

Sequences:

>Translated_259_residues
MAAIQITQAGPLLTLTLSRPEVRNAFNDEVIAELTAAFAQAGRREDVRAVVLAAEGPAFCAGADLNWMRRMADYTREENL
ADAAQLAEMLRAIYECPKPTVARIQGDVYAGGMGLVAACDIAVSGDTAQYCLSEVKLGLIPATISPYVIRAMGARAAHRY
FLTAERFDAQEAHRIGFIHEVVSAVALDAKVAEIAQALVNASPQAVKACKRLVQDVAQHDITPALIAQTVQGIADIRSSE
QGKEGVQSFLQKRKPSWII
>Mature_258_residues
AAIQITQAGPLLTLTLSRPEVRNAFNDEVIAELTAAFAQAGRREDVRAVVLAAEGPAFCAGADLNWMRRMADYTREENLA
DAAQLAEMLRAIYECPKPTVARIQGDVYAGGMGLVAACDIAVSGDTAQYCLSEVKLGLIPATISPYVIRAMGARAAHRYF
LTAERFDAQEAHRIGFIHEVVSAVALDAKVAEIAQALVNASPQAVKACKRLVQDVAQHDITPALIAQTVQGIADIRSSEQ
GKEGVQSFLQKRKPSWII

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=28.6885245901639, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=29.3436293436293, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=248, Percent_Identity=22.9838709677419, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221136753, Length=248, Percent_Identity=22.9838709677419, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221307494, Length=248, Percent_Identity=22.9838709677419, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI116235487, Length=232, Percent_Identity=22.8448275862069, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=29.7297297297297, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=27.710843373494, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=27.2388059701493, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=29.0836653386454, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082183, Length=239, Percent_Identity=23.4309623430962, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=28.3422459893048, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=26.4227642276423, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=213, Percent_Identity=30.0469483568075, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=26.6393442622951, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=178, Percent_Identity=27.5280898876405, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=225, Percent_Identity=23.5555555555556, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=240, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=24.8826291079812, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=180, Percent_Identity=27.2222222222222, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=28.5123966942149, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=28.5123966942149, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=26.1044176706827, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=218, Percent_Identity=26.605504587156, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19920382, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27874; Mature: 27743

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIQITQAGPLLTLTLSRPEVRNAFNDEVIAELTAAFAQAGRREDVRAVVLAAEGPAFC
CCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
AGADLNWMRRMADYTREENLADAAQLAEMLRAIYECPKPTVARIQGDVYAGGMGLVAACD
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCHHHHHHH
IAVSGDTAQYCLSEVKLGLIPATISPYVIRAMGARAAHRYFLTAERFDAQEAHRIGFIHE
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VVSAVALDAKVAEIAQALVNASPQAVKACKRLVQDVAQHDITPALIAQTVQGIADIRSSE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QGKEGVQSFLQKRKPSWII
CHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAIQITQAGPLLTLTLSRPEVRNAFNDEVIAELTAAFAQAGRREDVRAVVLAAEGPAFC
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
AGADLNWMRRMADYTREENLADAAQLAEMLRAIYECPKPTVARIQGDVYAGGMGLVAACD
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCHHHHHHH
IAVSGDTAQYCLSEVKLGLIPATISPYVIRAMGARAAHRYFLTAERFDAQEAHRIGFIHE
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VVSAVALDAKVAEIAQALVNASPQAVKACKRLVQDVAQHDITPALIAQTVQGIADIRSSE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QGKEGVQSFLQKRKPSWII
CHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA