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The map label for this gene is ghrA [H]

Identifier: 91789994

GI number: 91789994

Start: 4370474

End: 4371412

Strand: Reverse

Name: ghrA [H]

Synonym: Bpro_4156

Alternate gene names: 91789994

Gene position: 4371412-4370474 (Counterclockwise)

Preceding gene: 91789995

Following gene: 91789993

Centisome position: 84.06

GC content: 64.43

Gene sequence:

>939_bases
ATGAATCCGATGCGCATCAGTTTTTGCTGTACCGACACAAAAGCCCAGCCGTGGCTCGAAGGCCTGCGCGCCGCCCTGCC
TGATTCGCAGGTTGAGCTGTGGTCGGCGGGCGCGGCGCTGGCTGACTACGCGGTCGTCTGGGCGCCACCGCAACAATTTC
TGGATGAGCAGACCCGGCTCAAAGGCATCTTCAACATTGGCGCTGGCGTGGATGCGCTGATGAAACTGCGCCTGCCAGCA
GGCATACCGGTCGTCCGGCTGGACGATGCGGGCATGTCGGTGCAGATGGCCGAGTATGTCTGCCATGCCGTGATTCGCCA
TTTCCGCGAGTTCGACGGTTATGAGGCCGATGCGGCGCAGGGCAAATGGTCGTACCGGAAAACCCGCCTGCGCAAGGACT
TTCCGGTTGGCGTGATGGGGCTCGGTGTACTGGGCGAGCGCGTGGGCCAGGCCCTGGCGCAGTTTGACTTTCCGGTGATG
GGCTGGAGCCGCTCGGCAAAAAACGTGGCCGGCATGCAATGCTTTGCCGGCGAGGCGGGGTTTCCTGAATTCCTGGCGGC
CACCCGGATTTTGGTCTGCCTGCTGCCGCTGACGCCGGACACCGAGGGCATCATGCGGCGCGACACGCTGGCCCGGCTGC
AGCCCGGCGCCTATGTGATCAACGTGGCGCGGGGCGGCCATCTGGTGGACGAAGACCTGATCGCCCTGATTGACAGCGGC
CACATCGCGGGCGCCATGCTGGATGTGTTCCGCACGGAGCCGCTGCCCGCCTCGCATCCATTCTGGGCGCATCCCCGCAT
CACCGTGACGCCGCACACCTCGGCCCGAACGCTGCGCGAAGAGAGCATTGCGCAGATTGCGGGGAAGATACGCGCGCTGC
ACAGTGGCACGCCGATTGCCAGCCTTGCCGGTGTGGTTGACCCAAAAAAAGGATACTGA

Upstream 100 bases:

>100_bases
GCGCCGGCCGACGGCGTGGTGCAGGAATTGCTCTACGCGCCGGGCGATCAGGTCGCCGAAGGCGCGGAATTGCTGAAAAT
AGCGGTTTGAATTTTCATGG

Downstream 100 bases:

>100_bases
TGTTGCCCCCACGGTCGTTCTCTTCGCGTAACTCCCTGCCCCCGAGGGGCCCGCTGCGCCTGCGGCCCGGCCCTTCGACA
CGCTCAGGACAGGCCAAGGC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNPMRISFCCTDTKAQPWLEGLRAALPDSQVELWSAGAALADYAVVWAPPQQFLDEQTRLKGIFNIGAGVDALMKLRLPA
GIPVVRLDDAGMSVQMAEYVCHAVIRHFREFDGYEADAAQGKWSYRKTRLRKDFPVGVMGLGVLGERVGQALAQFDFPVM
GWSRSAKNVAGMQCFAGEAGFPEFLAATRILVCLLPLTPDTEGIMRRDTLARLQPGAYVINVARGGHLVDEDLIALIDSG
HIAGAMLDVFRTEPLPASHPFWAHPRITVTPHTSARTLREESIAQIAGKIRALHSGTPIASLAGVVDPKKGY

Sequences:

>Translated_312_residues
MNPMRISFCCTDTKAQPWLEGLRAALPDSQVELWSAGAALADYAVVWAPPQQFLDEQTRLKGIFNIGAGVDALMKLRLPA
GIPVVRLDDAGMSVQMAEYVCHAVIRHFREFDGYEADAAQGKWSYRKTRLRKDFPVGVMGLGVLGERVGQALAQFDFPVM
GWSRSAKNVAGMQCFAGEAGFPEFLAATRILVCLLPLTPDTEGIMRRDTLARLQPGAYVINVARGGHLVDEDLIALIDSG
HIAGAMLDVFRTEPLPASHPFWAHPRITVTPHTSARTLREESIAQIAGKIRALHSGTPIASLAGVVDPKKGY
>Mature_312_residues
MNPMRISFCCTDTKAQPWLEGLRAALPDSQVELWSAGAALADYAVVWAPPQQFLDEQTRLKGIFNIGAGVDALMKLRLPA
GIPVVRLDDAGMSVQMAEYVCHAVIRHFREFDGYEADAAQGKWSYRKTRLRKDFPVGVMGLGVLGERVGQALAQFDFPVM
GWSRSAKNVAGMQCFAGEAGFPEFLAATRILVCLLPLTPDTEGIMRRDTLARLQPGAYVINVARGGHLVDEDLIALIDSG
HIAGAMLDVFRTEPLPASHPFWAHPRITVTPHTSARTLREESIAQIAGKIRALHSGTPIASLAGVVDPKKGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=28.1954887218045, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI6912396, Length=171, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI87081824, Length=316, Percent_Identity=42.4050632911392, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI87082289, Length=148, Percent_Identity=31.0810810810811, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=258, Percent_Identity=28.2945736434109, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=24.0566037735849, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921140, Length=260, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574286, Length=235, Percent_Identity=26.8085106382979, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45552429, Length=260, Percent_Identity=25.3846153846154, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45551003, Length=260, Percent_Identity=25.3846153846154, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574284, Length=260, Percent_Identity=25.3846153846154, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585514, Length=260, Percent_Identity=25.3846153846154, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574282, Length=260, Percent_Identity=25.3846153846154, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33960; Mature: 33960

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPMRISFCCTDTKAQPWLEGLRAALPDSQVELWSAGAALADYAVVWAPPQQFLDEQTRL
CCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHCCEEEECCHHHHHHHHHHH
KGIFNIGAGVDALMKLRLPAGIPVVRLDDAGMSVQMAEYVCHAVIRHFREFDGYEADAAQ
HHHHHCCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKWSYRKTRLRKDFPVGVMGLGVLGERVGQALAQFDFPVMGWSRSAKNVAGMQCFAGEAG
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
FPEFLAATRILVCLLPLTPDTEGIMRRDTLARLQPGAYVINVARGGHLVDEDLIALIDSG
CHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
HIAGAMLDVFRTEPLPASHPFWAHPRITVTPHTSARTLREESIAQIAGKIRALHSGTPIA
CHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
SLAGVVDPKKGY
HHHHCCCCCCCC
>Mature Secondary Structure
MNPMRISFCCTDTKAQPWLEGLRAALPDSQVELWSAGAALADYAVVWAPPQQFLDEQTRL
CCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCCHHHCCEEEECCHHHHHHHHHHH
KGIFNIGAGVDALMKLRLPAGIPVVRLDDAGMSVQMAEYVCHAVIRHFREFDGYEADAAQ
HHHHHCCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GKWSYRKTRLRKDFPVGVMGLGVLGERVGQALAQFDFPVMGWSRSAKNVAGMQCFAGEAG
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
FPEFLAATRILVCLLPLTPDTEGIMRRDTLARLQPGAYVINVARGGHLVDEDLIALIDSG
CHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
HIAGAMLDVFRTEPLPASHPFWAHPRITVTPHTSARTLREESIAQIAGKIRALHSGTPIA
CHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
SLAGVVDPKKGY
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA