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The map label for this gene is proP [H]

Identifier: 91789980

GI number: 91789980

Start: 4354251

End: 4355909

Strand: Reverse

Name: proP [H]

Synonym: Bpro_4142

Alternate gene names: 91789980

Gene position: 4355909-4354251 (Counterclockwise)

Preceding gene: 91789982

Following gene: 91789979

Centisome position: 83.76

GC content: 59.43

Gene sequence:

>1659_bases
ATGTCAACAGCAGTCGACAACCGACCCATGACAGCTGAGCAGAAGAAAGTGATCTTCGCCTCATCGCTGGGCACCGTATT
TGAGTGGTATGACTTTTACCTCTACGGTTCACTCGCCGCCATCATTGCCAAGCAGTTCTTCAGCGGACTGGACGCCGGCT
CGGCGTTCATTTTTGCACTGCTGGCATTCGCCGCCGGTTTCATCGTGCGTCCCTTCGGCGCGCTGGTGTTCGGCCGCCTC
GGCGACATGATTGGACGCAAGTACACCTTCCTGGTCACCATCGTGATCATGGGCCTGTCGACCTTCATCGTGGGCTTGCT
GCCCAGCTATGCCTCCATCGGCGTTGCCGCTCCGGTGATCCTGATCGCATTGCGCATGTTGCAGGGCCTGGCACTCGGTG
GCGAGTACGGCGGTGCGGCGGTGTATGTGGCCGAGCATGCGCCGGCACACAAGCGCGGTGCTTATACCTCGTGGATTCAG
ACCACGGCAACGCTGGGTCTCTTCCTGTCGCTGATGGTGATTCTGGGCACACGCACCGCCATTGGCGAGGCCGCCTTTGC
CGACTGGGGCTGGCGTGTTCCCTTCATCGTCTCGATCCTGTTGCTGGCCGTGTCTGTGTACATCCGCCTGTCCATGAACG
AGTCGCCTGCCTTCCAGAAAATGAAGGCAGAGGGCAAGACCTCCAAGGCGCCGCTGTCCGAATCTTTCGGCCAGTGGAAA
AACCTGAAGATCGTGATTCTGGCGCTGATCGGTTTGACCGCCGGCCAGGCCGTGGTCTGGTACTCCGGCCAGTTTTATGC
GCTGTTCTTCCTGACGCAGGCGCTCAAGGTCGACGGTCCTACCGCCAACGTTCTGGTGGCTTATTCGCTGATCCTGGGCA
CGCCGTTTTTCGTCGTGTTCGGCGCGCTGTCTGACAAGATTGGCCGCAAGCCCATCATCATGGCAGGCTGTCTGCTCGCG
GTGATTACCTACTTCCCGGTGTTCGGCGCACTGACCAAAGCCGCCAATCCTGATCTGGCTGCAGCACAAGCCAAGAACAA
GGTGGTGGTGACTGCAGATACGAAAGAGTGTTCGTTCCAGTTCAACCCGACCGGTACGGCCAAGTTCACCAGCTCCTGCG
ACATCGCCAAGCAGGTGCTGGCCGGGGCATCGGTGAGCTATGAAAACGCCGAAGCGCCTGCAGGTACTGTCGCCACCATC
AAGATTGGCGAAACCGCCATTCCCAGCTACTCCTCCAAAGGCCTGAGCGCCGACGAAGCCAAGAAGAAGGACGCCGAGTT
CAAGAAGGCGGTTGCGGATGACCTGAAGGCCGCCGGCTACCCCAGCAAGGCAGACCCCGCCAAGGTGGATAAGGTCATGG
TCACGGCCATCCTGTTCTATCTGGTTCTACTGGTGACCATGGTGTACGGACCGATCGCCGCCATGCTGGTGGAGCTGTTT
CCCACCCGTATTCGCTACACGTCCATGAGCCTGCCTTATCACATCGGCAATGGCTGGTTCGGCGGCTTGCTGCCGACCAC
CGCCTTCGCCATCGTGGCCCAGACCGGCAACATGTACAACGGCCTGTGGTACCCGATCATCATTGCCGGCGCTACCTTTG
TGATCGGCACCCTGTTCATCAGGGAAACCAAGAACGTTGACATCTACGCCAACGACTGA

Upstream 100 bases:

>100_bases
AAGCTCCTGTTGGTTTCACGTCAGAGCTGGGTCAGCGGTCAGCAAAATAATGGACTCAGCTTCCACGTCGGAAGCCCTAC
TATTTTGGAGACAAACCATC

Downstream 100 bases:

>100_bases
CTGATCTTGAGGCGGCGCGGGCCCTGGCTGCCCGTGCTGACCAGAACGATTCCGTAAATTCAAAGGCCTTCGTCATCATC
TGCCGGGGGCCTTTTTTTGG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSTAVDNRPMTAEQKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRL
GDMIGRKYTFLVTIVIMGLSTFIVGLLPSYASIGVAAPVILIALRMLQGLALGGEYGGAAVYVAEHAPAHKRGAYTSWIQ
TTATLGLFLSLMVILGTRTAIGEAAFADWGWRVPFIVSILLLAVSVYIRLSMNESPAFQKMKAEGKTSKAPLSESFGQWK
NLKIVILALIGLTAGQAVVWYSGQFYALFFLTQALKVDGPTANVLVAYSLILGTPFFVVFGALSDKIGRKPIIMAGCLLA
VITYFPVFGALTKAANPDLAAAQAKNKVVVTADTKECSFQFNPTGTAKFTSSCDIAKQVLAGASVSYENAEAPAGTVATI
KIGETAIPSYSSKGLSADEAKKKDAEFKKAVADDLKAAGYPSKADPAKVDKVMVTAILFYLVLLVTMVYGPIAAMLVELF
PTRIRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIIAGATFVIGTLFIRETKNVDIYAND

Sequences:

>Translated_552_residues
MSTAVDNRPMTAEQKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRL
GDMIGRKYTFLVTIVIMGLSTFIVGLLPSYASIGVAAPVILIALRMLQGLALGGEYGGAAVYVAEHAPAHKRGAYTSWIQ
TTATLGLFLSLMVILGTRTAIGEAAFADWGWRVPFIVSILLLAVSVYIRLSMNESPAFQKMKAEGKTSKAPLSESFGQWK
NLKIVILALIGLTAGQAVVWYSGQFYALFFLTQALKVDGPTANVLVAYSLILGTPFFVVFGALSDKIGRKPIIMAGCLLA
VITYFPVFGALTKAANPDLAAAQAKNKVVVTADTKECSFQFNPTGTAKFTSSCDIAKQVLAGASVSYENAEAPAGTVATI
KIGETAIPSYSSKGLSADEAKKKDAEFKKAVADDLKAAGYPSKADPAKVDKVMVTAILFYLVLLVTMVYGPIAAMLVELF
PTRIRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIIAGATFVIGTLFIRETKNVDIYAND
>Mature_551_residues
STAVDNRPMTAEQKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFALLAFAAGFIVRPFGALVFGRLG
DMIGRKYTFLVTIVIMGLSTFIVGLLPSYASIGVAAPVILIALRMLQGLALGGEYGGAAVYVAEHAPAHKRGAYTSWIQT
TATLGLFLSLMVILGTRTAIGEAAFADWGWRVPFIVSILLLAVSVYIRLSMNESPAFQKMKAEGKTSKAPLSESFGQWKN
LKIVILALIGLTAGQAVVWYSGQFYALFFLTQALKVDGPTANVLVAYSLILGTPFFVVFGALSDKIGRKPIIMAGCLLAV
ITYFPVFGALTKAANPDLAAAQAKNKVVVTADTKECSFQFNPTGTAKFTSSCDIAKQVLAGASVSYENAEAPAGTVATIK
IGETAIPSYSSKGLSADEAKKKDAEFKKAVADDLKAAGYPSKADPAKVDKVMVTAILFYLVLLVTMVYGPIAAMLVELFP
TRIRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIIAGATFVIGTLFIRETKNVDIYAND

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=326, Percent_Identity=37.7300613496933, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1788292, Length=349, Percent_Identity=31.5186246418338, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789941, Length=335, Percent_Identity=35.8208955223881, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788942, Length=335, Percent_Identity=30.7462686567164, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=26.271186440678, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082404, Length=222, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323653, Length=164, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59005; Mature: 58874

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVDNRPMTAEQKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFAL
CCCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTIVIMGLSTFIVGLLPSYASIGVAAPVI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIALRMLQGLALGGEYGGAAVYVAEHAPAHKRGAYTSWIQTTATLGLFLSLMVILGTRTA
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
IGEAAFADWGWRVPFIVSILLLAVSVYIRLSMNESPAFQKMKAEGKTSKAPLSESFGQWK
HCCHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCHHHHCCCC
NLKIVILALIGLTAGQAVVWYSGQFYALFFLTQALKVDGPTANVLVAYSLILGTPFFVVF
CHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHH
GALSDKIGRKPIIMAGCLLAVITYFPVFGALTKAANPDLAAAQAKNKVVVTADTKECSFQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCEEE
FNPTGTAKFTSSCDIAKQVLAGASVSYENAEAPAGTVATIKIGETAIPSYSSKGLSADEA
ECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
KKKDAEFKKAVADDLKAAGYPSKADPAKVDKVMVTAILFYLVLLVTMVYGPIAAMLVELF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIIAGATFVIGTLFI
HHHHEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RETKNVDIYAND
EECCCEEEEECC
>Mature Secondary Structure 
STAVDNRPMTAEQKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDAGSAFIFAL
CCCCCCCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTIVIMGLSTFIVGLLPSYASIGVAAPVI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIALRMLQGLALGGEYGGAAVYVAEHAPAHKRGAYTSWIQTTATLGLFLSLMVILGTRTA
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
IGEAAFADWGWRVPFIVSILLLAVSVYIRLSMNESPAFQKMKAEGKTSKAPLSESFGQWK
HCCHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCHHHHCCCC
NLKIVILALIGLTAGQAVVWYSGQFYALFFLTQALKVDGPTANVLVAYSLILGTPFFVVF
CHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHH
GALSDKIGRKPIIMAGCLLAVITYFPVFGALTKAANPDLAAAQAKNKVVVTADTKECSFQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCEEE
FNPTGTAKFTSSCDIAKQVLAGASVSYENAEAPAGTVATIKIGETAIPSYSSKGLSADEA
ECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
KKKDAEFKKAVADDLKAAGYPSKADPAKVDKVMVTAILFYLVLLVTMVYGPIAAMLVELF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGLLPTTAFAIVAQTGNMYNGLWYPIIIAGATFVIGTLFI
HHHHEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RETKNVDIYAND
EECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]