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The map label for this gene is fcl [C]

Identifier: 91789840

GI number: 91789840

Start: 4197234

End: 4198229

Strand: Direct

Name: fcl [C]

Synonym: Bpro_4000

Alternate gene names: 91789840

Gene position: 4197234-4198229 (Clockwise)

Preceding gene: 91789839

Following gene: 91789841

Centisome position: 80.71

GC content: 51.81

Gene sequence:

>996_bases
ATGATCAACATATCGGGATCAAGTCCTGCATACGAGGGGATTTCTTCGTTTGCAGGCAAAAAGACGTGGATAACCGGACA
TCGCGGAATGCTTGGATCTGCCCTGGTTCGCAGCTTTGCCAAAGAGGATGCCAATATATTGCTGACCTCTCGCCAGGAAC
TTGACTTGTCCGACCAAAGCGCCGTCTTCAATTGGGTCGACGAAAACCGGCCTGAGTTGATTTTTCACGTGGGTGCAAAG
GTAGGGGGTATCCACGCCAACTCCACGCTTCCTGCCGATTTTCTGTACAGCAATTTGATGATTCAATCAAATGTCATCAA
TGCAGCGCATCTTTTTGGGGCGAAGAAACTGGTGTTCGTGGCATCAAATTGCACCTACCCAACAAAAACAGCACAGCCTA
TACCCGAAGAAGCGCTGATGACCGGCCCTCTCGACGAGAACATTCGCGCTTATGCCATCAGCAAAATCGCGGGCATTGAG
ATGTGCCGAGCCTATCGCAAGCAGTATGGCTCCAACTTTATCTCCGTAATTCCTCCCAATTTGTATGGTCCTGGTGATAA
CTATCACCCACAGCATAGCCACGTCGTTGCGGGCATTTTGCGCCGTACCCACGAGGCAAAGCTGGCCGGCAAATCAGAGT
TTGTTGTTTGGGGTGACGGCACTCCGCGACGTGAATTGTTGCACGTGGACGACCTGGCTGATGCCATGAAGACCCTCATG
GCTGCCCCCGTCACTCATGATCTGTACAACATTGGCTGCGGCCATGACTTGTCAATTGCGGAGCTGGCGACGCTGATTGC
CGAAGTAGTCGACTTCAAGGGAAGCATCGTCTACGACAAATCCAAGCCAAATGGCACGATGAGAAAATTATTGGACAGCT
CACGTATTCACTCTTTGGGCTGGCGTCCAAAAATAGATGAGAAGGCCGGCCTCAAGAGTGCCTACAGGGACTTCCTGACC
CTTCTCGAAACACCGGAACTTGCCGGGCGGCTGTAA

Upstream 100 bases:

>100_bases
CGCCTGAAAACATGGAATGATTGCCGCCGATCAAACGTAATCGAATGTCACAATTGGGCGTTAGCTTCGACAAAATGTAA
ATGTATTGGTGAATAGATTA

Downstream 100 bases:

>100_bases
TGTCCACGCTTGACGGCGGCGGGGCCGTACAAGGCAAATGAATTTCCGGTCAATTTCCGGTCGTATCACTGCAATGGGCT
ACTAACCTCCGCTTGAGATT

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MINISGSSPAYEGISSFAGKKTWITGHRGMLGSALVRSFAKEDANILLTSRQELDLSDQSAVFNWVDENRPELIFHVGAK
VGGIHANSTLPADFLYSNLMIQSNVINAAHLFGAKKLVFVASNCTYPTKTAQPIPEEALMTGPLDENIRAYAISKIAGIE
MCRAYRKQYGSNFISVIPPNLYGPGDNYHPQHSHVVAGILRRTHEAKLAGKSEFVVWGDGTPRRELLHVDDLADAMKTLM
AAPVTHDLYNIGCGHDLSIAELATLIAEVVDFKGSIVYDKSKPNGTMRKLLDSSRIHSLGWRPKIDEKAGLKSAYRDFLT
LLETPELAGRL

Sequences:

>Translated_331_residues
MINISGSSPAYEGISSFAGKKTWITGHRGMLGSALVRSFAKEDANILLTSRQELDLSDQSAVFNWVDENRPELIFHVGAK
VGGIHANSTLPADFLYSNLMIQSNVINAAHLFGAKKLVFVASNCTYPTKTAQPIPEEALMTGPLDENIRAYAISKIAGIE
MCRAYRKQYGSNFISVIPPNLYGPGDNYHPQHSHVVAGILRRTHEAKLAGKSEFVVWGDGTPRRELLHVDDLADAMKTLM
AAPVTHDLYNIGCGHDLSIAELATLIAEVVDFKGSIVYDKSKPNGTMRKLLDSSRIHSLGWRPKIDEKAGLKSAYRDFLT
LLETPELAGRL
>Mature_331_residues
MINISGSSPAYEGISSFAGKKTWITGHRGMLGSALVRSFAKEDANILLTSRQELDLSDQSAVFNWVDENRPELIFHVGAK
VGGIHANSTLPADFLYSNLMIQSNVINAAHLFGAKKLVFVASNCTYPTKTAQPIPEEALMTGPLDENIRAYAISKIAGIE
MCRAYRKQYGSNFISVIPPNLYGPGDNYHPQHSHVVAGILRRTHEAKLAGKSEFVVWGDGTPRRELLHVDDLADAMKTLM
AAPVTHDLYNIGCGHDLSIAELATLIAEVVDFKGSIVYDKSKPNGTMRKLLDSSRIHSLGWRPKIDEKAGLKSAYRDFLT
LLETPELAGRL

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=281, Percent_Identity=30.9608540925267, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=21.301775147929, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788365, Length=312, Percent_Identity=47.1153846153846, Blast_Score=297, Evalue=7e-82,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=23.2758620689655, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=25, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=286, Percent_Identity=26.5734265734266, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=23.3333333333333, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922778, Length=292, Percent_Identity=31.1643835616438, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 36291; Mature: 36291

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINISGSSPAYEGISSFAGKKTWITGHRGMLGSALVRSFAKEDANILLTSRQELDLSDQS
CEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCH
AVFNWVDENRPELIFHVGAKVGGIHANSTLPADFLYSNLMIQSNVINAAHLFGAKKLVFV
HHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCEEEEE
ASNCTYPTKTAQPIPEEALMTGPLDENIRAYAISKIAGIEMCRAYRKQYGSNFISVIPPN
ECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LYGPGDNYHPQHSHVVAGILRRTHEAKLAGKSEFVVWGDGTPRRELLHVDDLADAMKTLM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHEEHHHHHHHHHHHH
AAPVTHDLYNIGCGHDLSIAELATLIAEVVDFKGSIVYDKSKPNGTMRKLLDSSRIHSLG
HCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCC
WRPKIDEKAGLKSAYRDFLTLLETPELAGRL
CCCCCCHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MINISGSSPAYEGISSFAGKKTWITGHRGMLGSALVRSFAKEDANILLTSRQELDLSDQS
CEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCH
AVFNWVDENRPELIFHVGAKVGGIHANSTLPADFLYSNLMIQSNVINAAHLFGAKKLVFV
HHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCEEEEE
ASNCTYPTKTAQPIPEEALMTGPLDENIRAYAISKIAGIEMCRAYRKQYGSNFISVIPPN
ECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LYGPGDNYHPQHSHVVAGILRRTHEAKLAGKSEFVVWGDGTPRRELLHVDDLADAMKTLM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHEEHHHHHHHHHHHH
AAPVTHDLYNIGCGHDLSIAELATLIAEVVDFKGSIVYDKSKPNGTMRKLLDSSRIHSLG
HCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCC
WRPKIDEKAGLKSAYRDFLTLLETPELAGRL
CCCCCCHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]