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The map label for this gene is trpB

Identifier: 91789467

GI number: 91789467

Start: 3818995

End: 3820338

Strand: Direct

Name: trpB

Synonym: Bpro_3616

Alternate gene names: 91789467

Gene position: 3818995-3820338 (Clockwise)

Preceding gene: 91789466

Following gene: 91789468

Centisome position: 73.44

GC content: 62.87

Gene sequence:

>1344_bases
ATGTTCGACTACCACCAACCTGACCCGACCGGCCATTTCGGCATTTACGGCGGCAGTTTCGTTTCCGAGACACTGACCCA
CGCGATCAATGAGCTGAAAGACGCCTACGCGAAGTACCAGAACGACCCGGAATTCCTGGCCGAATTCCATTCAGAGCTGG
CCCACTTCGTGGGCCGTCCCTCTCCCATTTACCATGCGGCCCGCATGAGCCGCGAACAGGGCGGTGCGCAGATCTACCTG
AAGCGCGAAGACCTGAACCATACCGGCGCGCACAAGATCAACAACACCATCGGCCAGGCCATGCTGGCCCGCCGCATGGG
CAAGACGCGCATCATTGCTGAAACCGGCGCCGGCCAGCACGGCGTGGCAACCGCCACCATCTGCGCGCGTTATGGCCTGG
AATGCGTGGTCTACATGGGCAGCGAAGACGTCAAGCGCCAGAGCCCCAATGTCTACCGCATGAAGCTGCTGGGCGCCACG
GTGGTTCCGGTTGAAAGCGGCAGCAAGACGCTGAAAGATGCACTCAACGAGGCCATGCGCGACTGGGTCGCCAATGTGGA
CAACACCTTCTACATCATCGGCACCGTGGCGGGGCCGCACCCCTACCCCATGATGGTCCGCGACTTCCAGAGCGTGATCG
GTACCGAATGCCTGGCGCAAATGCCGGAATTCATCGGTAACCGCCAGCCCGATGCGGTGATTGCCTGCGTCGGTGGGGGC
AGCAATGCCATGGGCATTTTCTACCCCTATATTGCGCATGAAGAAACCCGCCTGATCGGCGTGGAAGCGGCCGGCGAGGG
CCTGGAGAGCGGCAAGCACTCCGCATCGCTGCAGCGCGGCCTGCCCGGCGTGCTGCACGGCAACCGTACCTATGTCCTGC
AAAACGACGATGGCCAGGTGACCGAGACGCACAGCATCAGCGCCGGACTCGACTACCCCGGCGTGGGGCCCGAGCATGCC
TTCCTGAAGGACATTGGCCGCGCCGAATATGTGGGCATCACGGACACCGAAGCGCTGGAAGCCTTCCATTACCTGTGCCG
TACCGAGGGCATCATTGCCGCGCTGGAGTCCAGCCACGCCGTGGCCTACGCGATGAAACTTGCCAAAACCATGCGCAGCG
ACCAGAGCATCCTGGTCAACCTGTCGGGCCGGGGCGACAAGGACATCGGCACGGTGGCCGACCTCTCGGGCGCAGACTTT
TATGACCGGCCGTCGATGCGCGGCCTGGCCGTCAAGGGCGGCGAACAACCAAAGGAATTTTCCGACGGGCCGCCCCTAGG
AAAATTAGCCCCCTCGGGGGGCAGCGCAGTACGCGAAGCGACAAGCGTGGGGGCCAGGAAATGA

Upstream 100 bases:

>100_bases
GTCGAGGTTTCGAAAGGAATCAAGAGCGCCGACAAAATCAACCAATTTGTCGCAGCCGTACGCGCTGCCGACGAGCAACT
TGCGAAGACCCAAACCCATC

Downstream 100 bases:

>100_bases
GCCGCATTGCTTCTACCTTTTCCGCGCTGAAAAGCCAGGGCCGCAAGGCCCTGATTCCCTACGTCACCGCAGGCTTCCCC
TTCGCCGATATCACGCCTGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MFDYHQPDPTGHFGIYGGSFVSETLTHAINELKDAYAKYQNDPEFLAEFHSELAHFVGRPSPIYHAARMSREQGGAQIYL
KREDLNHTGAHKINNTIGQAMLARRMGKTRIIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGAT
VVPVESGSKTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGTECLAQMPEFIGNRQPDAVIACVGGG
SNAMGIFYPYIAHEETRLIGVEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQVTETHSISAGLDYPGVGPEHA
FLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVADLSGADF
YDRPSMRGLAVKGGEQPKEFSDGPPLGKLAPSGGSAVREATSVGARK

Sequences:

>Translated_447_residues
MFDYHQPDPTGHFGIYGGSFVSETLTHAINELKDAYAKYQNDPEFLAEFHSELAHFVGRPSPIYHAARMSREQGGAQIYL
KREDLNHTGAHKINNTIGQAMLARRMGKTRIIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGAT
VVPVESGSKTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGTECLAQMPEFIGNRQPDAVIACVGGG
SNAMGIFYPYIAHEETRLIGVEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQVTETHSISAGLDYPGVGPEHA
FLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVADLSGADF
YDRPSMRGLAVKGGEQPKEFSDGPPLGKLAPSGGSAVREATSVGARK
>Mature_447_residues
MFDYHQPDPTGHFGIYGGSFVSETLTHAINELKDAYAKYQNDPEFLAEFHSELAHFVGRPSPIYHAARMSREQGGAQIYL
KREDLNHTGAHKINNTIGQAMLARRMGKTRIIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGAT
VVPVESGSKTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGTECLAQMPEFIGNRQPDAVIACVGGG
SNAMGIFYPYIAHEETRLIGVEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQVTETHSISAGLDYPGVGPEHA
FLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHAVAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVADLSGADF
YDRPSMRGLAVKGGEQPKEFSDGPPLGKLAPSGGSAVREATSVGARK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=55.0649350649351, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321412, Length=394, Percent_Identity=54.3147208121827, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_POLSJ (Q126M1)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550419.1
- ProteinModelPortal:   Q126M1
- SMR:   Q126M1
- STRING:   Q126M1
- GeneID:   4013723
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3616
- NMPDR:   fig|296591.1.peg.5156
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q126M1
- ProtClustDB:   PRK04346
- BioCyc:   PSP296591:BPRO_3616-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 48182; Mature: 48182

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYHQPDPTGHFGIYGGSFVSETLTHAINELKDAYAKYQNDPEFLAEFHSELAHFVGRP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
SPIYHAARMSREQGGAQIYLKREDLNHTGAHKINNTIGQAMLARRMGKTRIIAETGAGQH
CHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGATVVPVESGSKTLKDALNEAMR
CHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHH
DWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGTECLAQMPEFIGNRQPDAVIACVGGG
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SNAMGIFYPYIAHEETRLIGVEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQV
CCCCEEEEHEECCCCCEEEEEECCCCCHHCCCHHHHHHHCCCCEEECCEEEEEECCCCCE
TETHSISAGLDYPGVGPEHAFLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHA
EEECCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCEEEHHCCHH
VAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVADLSGADFYDRPSMRGLAVKGGEQPKEF
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCC
SDGPPLGKLAPSGGSAVREATSVGARK
CCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MFDYHQPDPTGHFGIYGGSFVSETLTHAINELKDAYAKYQNDPEFLAEFHSELAHFVGRP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
SPIYHAARMSREQGGAQIYLKREDLNHTGAHKINNTIGQAMLARRMGKTRIIAETGAGQH
CHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGATVVPVESGSKTLKDALNEAMR
CHHHHHHHHHCCCEEEEEECCHHHHHCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHH
DWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGTECLAQMPEFIGNRQPDAVIACVGGG
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SNAMGIFYPYIAHEETRLIGVEAAGEGLESGKHSASLQRGLPGVLHGNRTYVLQNDDGQV
CCCCEEEEHEECCCCCEEEEEECCCCCHHCCCHHHHHHHCCCCEEECCEEEEEECCCCCE
TETHSISAGLDYPGVGPEHAFLKDIGRAEYVGITDTEALEAFHYLCRTEGIIAALESSHA
EEECCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCEEEHHCCHH
VAYAMKLAKTMRSDQSILVNLSGRGDKDIGTVADLSGADFYDRPSMRGLAVKGGEQPKEF
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCC
SDGPPLGKLAPSGGSAVREATSVGARK
CCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA