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The map label for this gene is gloA [H]

Identifier: 91789403

GI number: 91789403

Start: 3749469

End: 3749879

Strand: Direct

Name: gloA [H]

Synonym: Bpro_3549

Alternate gene names: 91789403

Gene position: 3749469-3749879 (Clockwise)

Preceding gene: 91789400

Following gene: 91789407

Centisome position: 72.1

GC content: 61.31

Gene sequence:

>411_bases
ATGCGACTACTCCACACCATGCTGCGCGTTGGCAATCTCCAGCGCTCCATCGACTTCTACACCCAGGTGCTCGGCATGAA
GCTGTTGCGCACCTCCGAAAACCCCGAATACAAGTACAGCCTGGCTTTCGTCGGCTATGAAGGCAACCCGGCGCAGGCCG
AGATTGAGCTGACCTACAACTGGGGCACCGAAAGCTATGAGCTGGGCACGGCCTATGGCCACATTGCGCTGGGCGTGCCG
GACGCTTATGCGGCCTGCGAAAAGATCAAGGCCGCGGGCGGCAATGTGACGCGCGAAGCCGGCCCCGTCAAGGGCGGCAA
GACGGTGATTGCCTTTGTGACCGACCCGGACGGCTACAAGATTGAGTTGATCCAGCGCGCTGAAGATGGCGGCGGCGCTG
GTTTGCGTTGA

Upstream 100 bases:

>100_bases
AAAGCGGCTTTCCGAAGGTCTGCCGGGCGACCGCGGCCAACCACTCCAGGGTGCTGAACACGCCCCTGCAAACTTCCCCC
GGTAGCGGGATAATCCGCGC

Downstream 100 bases:

>100_bases
GGCTTACAGGAGCTATATCTCATATAGCTCCTTGCGCCCATTTTTATTGGGCTAGAAGCTGATTTCGCTCATGAACGCCC
GAGTTTGAGTGTTCCCCGGC

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MRLLHTMLRVGNLQRSIDFYTQVLGMKLLRTSENPEYKYSLAFVGYEGNPAQAEIELTYNWGTESYELGTAYGHIALGVP
DAYAACEKIKAAGGNVTREAGPVKGGKTVIAFVTDPDGYKIELIQRAEDGGGAGLR

Sequences:

>Translated_136_residues
MRLLHTMLRVGNLQRSIDFYTQVLGMKLLRTSENPEYKYSLAFVGYEGNPAQAEIELTYNWGTESYELGTAYGHIALGVP
DAYAACEKIKAAGGNVTREAGPVKGGKTVIAFVTDPDGYKIELIQRAEDGGGAGLR
>Mature_136_residues
MRLLHTMLRVGNLQRSIDFYTQVLGMKLLRTSENPEYKYSLAFVGYEGNPAQAEIELTYNWGTESYELGTAYGHIALGVP
DAYAACEKIKAAGGNVTREAGPVKGGKTVIAFVTDPDGYKIELIQRAEDGGGAGLR

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=38.3561643835616, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=78.3582089552239, Blast_Score=213, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=41.4285714285714, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=40.2777777777778, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920404, Length=137, Percent_Identity=29.9270072992701, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14777; Mature: 14777

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGNLQRSIDFYTQVLGMKLLRTSENPEYKYSLAFVGYEGNPAQAEIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
WGTESYELGTAYGHIALGVPDAYAACEKIKAAGGNVTREAGPVKGGKTVIAFVTDPDGYK
CCCCCEEECCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIQRAEDGGGAGLR
EEEEEECCCCCCCCCC
>Mature Secondary Structure
MRLLHTMLRVGNLQRSIDFYTQVLGMKLLRTSENPEYKYSLAFVGYEGNPAQAEIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
WGTESYELGTAYGHIALGVPDAYAACEKIKAAGGNVTREAGPVKGGKTVIAFVTDPDGYK
CCCCCEEECCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIQRAEDGGGAGLR
EEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]