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The map label for this gene is nasA [H]

Identifier: 91789133

GI number: 91789133

Start: 3464423

End: 3465634

Strand: Direct

Name: nasA [H]

Synonym: Bpro_3273

Alternate gene names: 91789133

Gene position: 3464423-3465634 (Clockwise)

Preceding gene: 91789132

Following gene: 91789136

Centisome position: 66.62

GC content: 63.61

Gene sequence:

>1212_bases
ATGGCGGGATTCCGCACCTTTCTCAAATCGGGCCATGCTCCGACCTTGTTCGCCGCCTTCCTGTACTTCGATTTCTGCTT
TGCGGTCTGGGTATTGAATGGGGCCATGGCGCCGTTCATCAGCGAGACCTTTCATCTGACGGCGGCGCAGAAGGGCTTCA
TGATTTCGGTGCCCATTCTGGCGGGCGCCCTCATGCGCTTTCCGCTGGGCATTCTGGCGCAATACATCGGCCGCAAAAAT
GCTGCGCTGGTCGAAATGGGCATGATTATTCTGGCGCTGGCCTACGGCTACTTCTTCATCAGCGCGTACGACCATGTGCT
AGCCATGGGCGTGCTGCTGGGCATCGCCGGCGCGAGCTTCGGCGTGGCGCTGTCGCTGGGTTCGGGCTGGTTCCCGCCCA
AGTACAAGGGCCTGGCCATGGGCATTGCCGGCGCCGGCAACTCCGGCACCGTGCTAGCCGTGCTGTTCGCACCGCCACTG
GCCATGGTCTATGGCTGGCAGACGGTTTACGGCCTGGCGGCCTTCTTCATGCTGATCCCGATGGTGGTAATGGTCGTCTT
CGCGAAAGAGCCGCCCGACATCGAGCACCAGACCTTCCGCCAGCACATCGCCTGCCTGTTCGAGAAGGACGGCTGGGCGT
TCAGCCTGATCTACATCATCACTTTCGGCGGCTTCATCGGCCTGGCCAGCTTCCTGCCCACCTACTTCTACGACCAGTTC
GGCGTCACCAAGGTGCAGGCAGGCCAGCTCACCATGCTGGCAGCACTCATGGGCTCAGCCGTGCGCGTGGTCGGCGGCTA
CATCTCTGACCGCGTGGGCGGCATCAACACCCTGAGCGGCGTGCTGGTGGTGGTCGCCTTCACCCTGGTGCTGTGCGGCC
TGGCCGGCAATTCGCTCACCGTCACCACCCTGCTCTTCATGGTCTGCTTTGCAGCGCTCGGTGCCGGCAACGGCGCGCTG
TTCCAGCTGGTGCCGCTGCGCTGGCCGCTGGCCACGGCGGTGGCGGGCTCCATGATTGGCGAGGTGGGCGCTCTTGGGGG
CGGCTTCATTCCCAACGCCATGGGCCTGAGCAAGCAGTACACCAATACCTACCTCTGGGGCTTTGTGGCTTTCGCCATCC
TGGCCGTCGTCATGCTGGTGATGCTGCGGGTGATGCAAATCCGCTGGACCCGGACCTGGGCTGAAAAGGGAGGTCGCGCG
CGGAGCGCGTGA

Upstream 100 bases:

>100_bases
CTGAACTGCTCCGCAAGGGTGCGCCGCTCGCGCCCCAAGTTGGCACGACTTTTGCACTAACTTTGTGAATTTTTATTTCA
ATTCACAGAGGATTTGCACC

Downstream 100 bases:

>100_bases
TCAGCGCAGCCGATACGGCGCGCAGCGCCGAGCCTCCCTTTCAGTGGAGACCAACTTTGCTTCGGCAAAGTTAAGCGAAG
CGTGTCGGAACTAAAAGCGA

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAGFRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISETFHLTAAQKGFMISVPILAGALMRFPLGILAQYIGRKN
AALVEMGMIILALAYGYFFISAYDHVLAMGVLLGIAGASFGVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPL
AMVYGWQTVYGLAAFFMLIPMVVMVVFAKEPPDIEHQTFRQHIACLFEKDGWAFSLIYIITFGGFIGLASFLPTYFYDQF
GVTKVQAGQLTMLAALMGSAVRVVGGYISDRVGGINTLSGVLVVVAFTLVLCGLAGNSLTVTTLLFMVCFAALGAGNGAL
FQLVPLRWPLATAVAGSMIGEVGALGGGFIPNAMGLSKQYTNTYLWGFVAFAILAVVMLVMLRVMQIRWTRTWAEKGGRA
RSA

Sequences:

>Translated_403_residues
MAGFRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISETFHLTAAQKGFMISVPILAGALMRFPLGILAQYIGRKN
AALVEMGMIILALAYGYFFISAYDHVLAMGVLLGIAGASFGVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPL
AMVYGWQTVYGLAAFFMLIPMVVMVVFAKEPPDIEHQTFRQHIACLFEKDGWAFSLIYIITFGGFIGLASFLPTYFYDQF
GVTKVQAGQLTMLAALMGSAVRVVGGYISDRVGGINTLSGVLVVVAFTLVLCGLAGNSLTVTTLLFMVCFAALGAGNGAL
FQLVPLRWPLATAVAGSMIGEVGALGGGFIPNAMGLSKQYTNTYLWGFVAFAILAVVMLVMLRVMQIRWTRTWAEKGGRA
RSA
>Mature_402_residues
AGFRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISETFHLTAAQKGFMISVPILAGALMRFPLGILAQYIGRKNA
ALVEMGMIILALAYGYFFISAYDHVLAMGVLLGIAGASFGVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPLA
MVYGWQTVYGLAAFFMLIPMVVMVVFAKEPPDIEHQTFRQHIACLFEKDGWAFSLIYIITFGGFIGLASFLPTYFYDQFG
VTKVQAGQLTMLAALMGSAVRVVGGYISDRVGGINTLSGVLVVVAFTLVLCGLAGNSLTVTTLLFMVCFAALGAGNGALF
QLVPLRWPLATAVAGSMIGEVGALGGGFIPNAMGLSKQYTNTYLWGFVAFAILAVVMLVMLRVMQIRWTRTWAEKGGRAR
SA

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=427, Percent_Identity=24.3559718969555, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43002; Mature: 42871

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGFRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISETFHLTAAQKGFMISVPIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEHHHH
AGALMRFPLGILAQYIGRKNAALVEMGMIILALAYGYFFISAYDHVLAMGVLLGIAGASF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPLAMVYGWQTVYGLAAFFMLIP
HEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHH
MVVMVVFAKEPPDIEHQTFRQHIACLFEKDGWAFSLIYIITFGGFIGLASFLPTYFYDQF
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVTKVQAGQLTMLAALMGSAVRVVGGYISDRVGGINTLSGVLVVVAFTLVLCGLAGNSLT
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
VTTLLFMVCFAALGAGNGALFQLVPLRWPLATAVAGSMIGEVGALGGGFIPNAMGLSKQY
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHH
TNTYLWGFVAFAILAVVMLVMLRVMQIRWTRTWAEKGGRARSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]