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The map label for this gene is nasD [H]

Identifier: 91789136

GI number: 91789136

Start: 3469545

End: 3471977

Strand: Direct

Name: nasD [H]

Synonym: Bpro_3276

Alternate gene names: 91789136

Gene position: 3469545-3471977 (Clockwise)

Preceding gene: 91789133

Following gene: 91789137

Centisome position: 66.72

GC content: 63.13

Gene sequence:

>2433_bases
ATGAAAAAATCGAAACTGGTAATGGTCGGCAACGGCATGGCGGGGGTTCGCACCATCGAGGAATTGCTCAAGATCGCACC
CGAGCTGTACGACATCACCGTCTTCGGTGCCGAACCACACCCCAACTACAACCGCATTTTGCTCAGCCCCGTGCTGGCCG
GGGAGCAGACGCTCGACGAGATCGTGCTCAACAGCTGGGACTGGTACAAGGACAACCACATCACGCTGCACGCCGGCAAG
AAGGTCGTTGAAGTCGACCGCGTCCGCCGCGTCGTGCGCGCCGAGGACGGCACCGAAGAAGAATACGACCGATTGCTGAT
GTGCACCGGCTCCAACCCCTTCATCCTGCCGGTGCCCGGCAAAGACCTGCAGGGCGTGATTGCCTACCGCGACATTGCCG
ATACCAACGCCATGATTGAGGCGGCGCAGAAGTACAAAAAGGCCGTGGTGATCGGTGGCGGCCTGCTGGGCCTGGAAGCA
GCCAACGGCCTGATGCTGCGCGGCATGGAGGTCACCGTGGTGCACGTCATGCCCTGGCTGATGGAGCGCCAGCTCGACGA
TGTGGCGGGCAAACTGCTGCAAAAGTCGCTGGAAGACCGCGGCCTCAAGTTCCTGATCGGCGCGCAAACCCAGGAACTGA
CCGGCGGCGCCGATGGCCGTGTCAAGTCCATCAAGTTCAAGGACGGCAGCGAGCTCGACACCGATCTCGTGGTGATGGCC
GTGGGCATCCGCCCCAACACCGAGCTGGCCGAAAAGATGCGCCTGCACTGCAACCGCGGCATCGTCGTCAACGACACCAT
GCAGACCGTGACCGATGCCCGCATCTATTCCGTCGGCGAGTGCGCTGCGCACCGCGGCATCGCCTACGGCCTGGTGGCGC
CGCTGTTCGAGCAGGCCAAGGTAGCCGCCAATCACCTGGCGCAATTCGGTATTGGCCGCTATACCGGCTCCCTGACGTCC
ACCAAGCTCAAGGTCACCGGCATTGACCTGTTCAGCGCCGGCGAATTCATGGGCGGCGAGGGCACCGAGGAGATCGTGAT
GAGCGACCCCTTCGGCGGGGTTTACAAGAAACTGGTCATCAAGGGCGACAAGCTGATTGGCGCCTGCCTGTACGGCGACA
CCGTCGACGGCAGCTATTACTTCAAGCTGCTGCGCGATGGGCGCAGCGTCAGCGACATCCGCGACAAGCTGATGTTTGGC
GAATCCAATATCGGCGACACCGGCCACGAAGGCCACAGCAAGGCAGCCGCCATGCCCGATGACGCCGAGGTCTGCGGCTG
CAACGGTGTCACCAAAGGAACCATCTGCAAGGCTATCAAGGAAAAAGGCCTGTTCACACTCGACGAAGTGCGCAAGCACA
CCAAGGCCAGCGCTTCGTGCGGCTCCTGCACCGGCCTGGTCGAGCAGCTCCTGATGTTCACGGCCGGTGGCGACTACTCG
GCCACCCCCAAGAAAAAGGCCGTGTGCGGCTGCACCGACCACAGCCATCAGGAGGTGCGCGACACCATCAAGTCGCTGCA
CCTTCTGACCATCGCTGACGTTTACAAGACCATGGAGTGGCGCACGCCCAACGGCTGCGCGTCCTGCCGCCCCGCCATCA
ACTACTACCTGATCAGCACCTGGCCCAAGGAGGCCAAGGATGACCCGCAAAGCCGCTACATCAACGAACGCAGCCACGCC
AACATACAGCGGGACGGCACCTACAGCGTGATCCCGCGCATGTGGGGCGGCCACACCACGCCGAACGACCTGCGTCGCAT
CGCCGACGTGGCCGACAAGTACCAGATTCCCACCGTCAAGGTGACGGGCGGCCAGCGCATCGACCTGCTGGGCGTGAAGA
AGGAAGACCTGCAGAGCGTCTGGCAGGACCTCGGCATGCCCAGTGGCCTGGCCTATGCCAAGGCGACGCGCACGGTGAAG
ACCTGCGTGGGCAGCGAATGGTGCCGCTTCGGCACGCAGGACTCTACGCAGATGGGCAAGGACCTGGAGCACGCGCTCTG
GGCCATGTATGCACCGCACAAGGTCAAGCTGGCGGTCAGCGGCTGTCCGCGCAACTGCGCCGAATCCGGCATCAAGGACG
TGGGCGTGATCGGCGTGGACTCCGGCTGGGAGATCTACGTGGGCGGCAACGGCGGCATCAAGACCGAGGTGGCGGAATTC
CTGGTGAAGGTCAAGACCTCCAGCGAGGTGATGGAATACGCCGGCGCCTTCCTGCAGCTCTACCGTGAGGAAGGCTGGTA
CCTGGAGCGTACGGTGCACTACCTGCACCGTGTCGGTCTCGACTACGTCAAGAAGAAGATTCTGGACGACGCCGAAGGCC
GCAAGGCCCTATGGGAGCGCCTGCAATTCAGCCTCGACGGAGCACCCGACCCCTGGCTCGAGACCAGCAAGGCAGCCGTG
GACACACGCCAGTTCGAGAAGTTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TTGTCACTGCTTGCCTCTGCGCTGCCGGCACTGCGTCGCGGCCAGTTTGGCATGCCGTTTGCACTTACAACATTGAATCT
GAATCCACTGGAGTGACCCC

Downstream 100 bases:

>100_bases
GCGCAGCACGGACGCACAACGGAGTACTCCTCATGAAGCGAAGAATTTTTATTGTGTCGGCGGCGTTGTTTGGCCTGACG
GCTCACTCGCAAGTGGCTGA

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MKKSKLVMVGNGMAGVRTIEELLKIAPELYDITVFGAEPHPNYNRILLSPVLAGEQTLDEIVLNSWDWYKDNHITLHAGK
KVVEVDRVRRVVRAEDGTEEEYDRLLMCTGSNPFILPVPGKDLQGVIAYRDIADTNAMIEAAQKYKKAVVIGGGLLGLEA
ANGLMLRGMEVTVVHVMPWLMERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELTGGADGRVKSIKFKDGSELDTDLVVMA
VGIRPNTELAEKMRLHCNRGIVVNDTMQTVTDARIYSVGECAAHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSLTS
TKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYKKLVIKGDKLIGACLYGDTVDGSYYFKLLRDGRSVSDIRDKLMFG
ESNIGDTGHEGHSKAAAMPDDAEVCGCNGVTKGTICKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYS
ATPKKKAVCGCTDHSHQEVRDTIKSLHLLTIADVYKTMEWRTPNGCASCRPAINYYLISTWPKEAKDDPQSRYINERSHA
NIQRDGTYSVIPRMWGGHTTPNDLRRIADVADKYQIPTVKVTGGQRIDLLGVKKEDLQSVWQDLGMPSGLAYAKATRTVK
TCVGSEWCRFGTQDSTQMGKDLEHALWAMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAEF
LVKVKTSSEVMEYAGAFLQLYREEGWYLERTVHYLHRVGLDYVKKKILDDAEGRKALWERLQFSLDGAPDPWLETSKAAV
DTRQFEKLPA

Sequences:

>Translated_810_residues
MKKSKLVMVGNGMAGVRTIEELLKIAPELYDITVFGAEPHPNYNRILLSPVLAGEQTLDEIVLNSWDWYKDNHITLHAGK
KVVEVDRVRRVVRAEDGTEEEYDRLLMCTGSNPFILPVPGKDLQGVIAYRDIADTNAMIEAAQKYKKAVVIGGGLLGLEA
ANGLMLRGMEVTVVHVMPWLMERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELTGGADGRVKSIKFKDGSELDTDLVVMA
VGIRPNTELAEKMRLHCNRGIVVNDTMQTVTDARIYSVGECAAHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSLTS
TKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYKKLVIKGDKLIGACLYGDTVDGSYYFKLLRDGRSVSDIRDKLMFG
ESNIGDTGHEGHSKAAAMPDDAEVCGCNGVTKGTICKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYS
ATPKKKAVCGCTDHSHQEVRDTIKSLHLLTIADVYKTMEWRTPNGCASCRPAINYYLISTWPKEAKDDPQSRYINERSHA
NIQRDGTYSVIPRMWGGHTTPNDLRRIADVADKYQIPTVKVTGGQRIDLLGVKKEDLQSVWQDLGMPSGLAYAKATRTVK
TCVGSEWCRFGTQDSTQMGKDLEHALWAMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAEF
LVKVKTSSEVMEYAGAFLQLYREEGWYLERTVHYLHRVGLDYVKKKILDDAEGRKALWERLQFSLDGAPDPWLETSKAAV
DTRQFEKLPA
>Mature_810_residues
MKKSKLVMVGNGMAGVRTIEELLKIAPELYDITVFGAEPHPNYNRILLSPVLAGEQTLDEIVLNSWDWYKDNHITLHAGK
KVVEVDRVRRVVRAEDGTEEEYDRLLMCTGSNPFILPVPGKDLQGVIAYRDIADTNAMIEAAQKYKKAVVIGGGLLGLEA
ANGLMLRGMEVTVVHVMPWLMERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELTGGADGRVKSIKFKDGSELDTDLVVMA
VGIRPNTELAEKMRLHCNRGIVVNDTMQTVTDARIYSVGECAAHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYTGSLTS
TKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYKKLVIKGDKLIGACLYGDTVDGSYYFKLLRDGRSVSDIRDKLMFG
ESNIGDTGHEGHSKAAAMPDDAEVCGCNGVTKGTICKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYS
ATPKKKAVCGCTDHSHQEVRDTIKSLHLLTIADVYKTMEWRTPNGCASCRPAINYYLISTWPKEAKDDPQSRYINERSHA
NIQRDGTYSVIPRMWGGHTTPNDLRRIADVADKYQIPTVKVTGGQRIDLLGVKKEDLQSVWQDLGMPSGLAYAKATRTVK
TCVGSEWCRFGTQDSTQMGKDLEHALWAMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAEF
LVKVKTSSEVMEYAGAFLQLYREEGWYLERTVHYLHRVGLDYVKKKILDDAEGRKALWERLQFSLDGAPDPWLETSKAAV
DTRQFEKLPA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=416, Percent_Identity=25.7211538461538, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI226437568, Length=270, Percent_Identity=28.5185185185185, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI65787454, Length=270, Percent_Identity=28.5185185185185, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI22202629, Length=228, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4757732, Length=228, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1789765, Length=833, Percent_Identity=36.0144057623049, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789065, Length=335, Percent_Identity=26.2686567164179, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788892, Length=287, Percent_Identity=25.4355400696864, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=412, Percent_Identity=25.7281553398058, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281359715, Length=309, Percent_Identity=25.2427184466019, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281359713, Length=309, Percent_Identity=25.2427184466019, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24639250, Length=309, Percent_Identity=25.2427184466019, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18543267, Length=309, Percent_Identity=25.2427184466019, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24639252, Length=309, Percent_Identity=25.2427184466019, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24639257, Length=309, Percent_Identity=25.2427184466019, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585130, Length=271, Percent_Identity=23.6162361623616, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21358499, Length=240, Percent_Identity=30, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581020, Length=297, Percent_Identity=26.5993265993266, Blast_Score=73, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28573993, Length=315, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89047; Mature: 89047

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00022 EGF_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSKLVMVGNGMAGVRTIEELLKIAPELYDITVFGAEPHPNYNRILLSPVLAGEQTLDE
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNSWDWYKDNHITLHAGKKVVEVDRVRRVVRAEDGTEEEYDRLLMCTGSNPFILPVPG
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCEEEECCC
KDLQGVIAYRDIADTNAMIEAAQKYKKAVVIGGGLLGLEAANGLMLRGMEVTVVHVMPWL
CCCCCEEEEHHHHHHHHHHHHHHHHCCEEEECCCEEEHHCCCCEEEECCEEEHHHHHHHH
MERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELTGGADGRVKSIKFKDGSELDTDLVVMA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCEEEEEECCCCCCCCCEEEEE
VGIRPNTELAEKMRLHCNRGIVVNDTMQTVTDARIYSVGECAAHRGIAYGLVAPLFEQAK
ECCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAANHLAQFGIGRYTGSLTSTKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYKKLVI
HHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHC
KGDKLIGACLYGDTVDGSYYFKLLRDGRSVSDIRDKLMFGESNIGDTGHEGHSKAAAMPD
CCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DAEVCGCNGVTKGTICKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYS
CCCEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
ATPKKKAVCGCTDHSHQEVRDTIKSLHLLTIADVYKTMEWRTPNGCASCRPAINYYLIST
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
WPKEAKDDPQSRYINERSHANIQRDGTYSVIPRMWGGHTTPNDLRRIADVADKYQIPTVK
CCCCCCCCCHHHHCCCHHCCCCCCCCCCEECCHHCCCCCCHHHHHHHHHHHHHCCCCEEE
VTGGQRIDLLGVKKEDLQSVWQDLGMPSGLAYAKATRTVKTCVGSEWCRFGTQDSTQMGK
ECCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
DLEHALWAMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAEF
HHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCHHHHHHH
LVKVKTSSEVMEYAGAFLQLYREEGWYLERTVHYLHRVGLDYVKKKILDDAEGRKALWER
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQFSLDGAPDPWLETSKAAVDTRQFEKLPA
HHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKSKLVMVGNGMAGVRTIEELLKIAPELYDITVFGAEPHPNYNRILLSPVLAGEQTLDE
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHHHCCHHHHHH
IVLNSWDWYKDNHITLHAGKKVVEVDRVRRVVRAEDGTEEEYDRLLMCTGSNPFILPVPG
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCEEEECCC
KDLQGVIAYRDIADTNAMIEAAQKYKKAVVIGGGLLGLEAANGLMLRGMEVTVVHVMPWL
CCCCCEEEEHHHHHHHHHHHHHHHHCCEEEECCCEEEHHCCCCEEEECCEEEHHHHHHHH
MERQLDDVAGKLLQKSLEDRGLKFLIGAQTQELTGGADGRVKSIKFKDGSELDTDLVVMA
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCEEEEEECCCCCCCCCEEEEE
VGIRPNTELAEKMRLHCNRGIVVNDTMQTVTDARIYSVGECAAHRGIAYGLVAPLFEQAK
ECCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAANHLAQFGIGRYTGSLTSTKLKVTGIDLFSAGEFMGGEGTEEIVMSDPFGGVYKKLVI
HHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHC
KGDKLIGACLYGDTVDGSYYFKLLRDGRSVSDIRDKLMFGESNIGDTGHEGHSKAAAMPD
CCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DAEVCGCNGVTKGTICKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLMFTAGGDYS
CCCEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
ATPKKKAVCGCTDHSHQEVRDTIKSLHLLTIADVYKTMEWRTPNGCASCRPAINYYLIST
CCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
WPKEAKDDPQSRYINERSHANIQRDGTYSVIPRMWGGHTTPNDLRRIADVADKYQIPTVK
CCCCCCCCCHHHHCCCHHCCCCCCCCCCEECCHHCCCCCCHHHHHHHHHHHHHCCCCEEE
VTGGQRIDLLGVKKEDLQSVWQDLGMPSGLAYAKATRTVKTCVGSEWCRFGTQDSTQMGK
ECCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHH
DLEHALWAMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAEF
HHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCHHHHHHH
LVKVKTSSEVMEYAGAFLQLYREEGWYLERTVHYLHRVGLDYVKKKILDDAEGRKALWER
HHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQFSLDGAPDPWLETSKAAVDTRQFEKLPA
HHHHCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]