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The map label for this gene is rbsB [H]

Identifier: 91789063

GI number: 91789063

Start: 3388672

End: 3389685

Strand: Direct

Name: rbsB [H]

Synonym: Bpro_3203

Alternate gene names: 91789063

Gene position: 3388672-3389685 (Clockwise)

Preceding gene: 91789060

Following gene: 91789064

Centisome position: 65.16

GC content: 64.2

Gene sequence:

>1014_bases
ATGAAACATACCGTTGCCCACACCATCGCCCGTACCGCCACCCTTCTCACGCTGTCCAGCCTTGCACTGGCCGCAACCTG
CGCATTCGCTGCGGGCGAGCCGGTGATCGGCCTGATCACCAAAACCGAAACCAATCCCTTCTTCGTCAAGATGAAGGAAG
GGGCCGAGGCCGAGGCCAAAAAACTCGGTGCCAAGCTGCTGACCGCGGCCGGAAAATCCGACGGCGACACCGCAGGCCAG
ATCACCGCCCTTGAAAACATGGCGGCTGCCGGCGCCAAGACCATTTTGATCACCGCCAGCGGCGATGCCATCGCCCCGAC
CGTCAAAAAACTGCAGGCCAAGGGCGTGCAGGTGATCGCGCTGGACAGCCCCTTTGCCGGCGCCGACGCGCTGTTTGCCA
CCGACAACTACCAGGCCGGCGTCCTGGTGGGCCAGTACGCCAAGGCGGCAATGGCCGGCAAACCCGCCCGTATCGTCACG
CTTGACCTGCTGCCCGGCCACCCGGTGGGCGCACAGCGGCACAACGGCTTCATGAAGGGTTTTGGCCTGCCTGCGGCAGA
CGCCAAGTCCAACGAACTGTCGCGGGACCCTGCCGTGGTGTGCATGGCCGACAGCTTTGGCGACCGGGCCAAGAGCCAGA
CCGCCATGGAGAACTGCCTGCAGAAAAATCCCGACATCAACGTGGTCTACACCATCAACGAACCCGCCGCCGCCGGCGCC
TTCAACGCGCTGAAGAAGGCCGGCAAGGAAAAAGGCGTGCTGATCCTGTCCGTCGACGGCGGCTGCCAGGGCATCAAGGA
TCTGGCGGCCGGCATGATCACTGCGACCTCGCAGCAATATCCGCTGAAGATGGCGGCACTGGGCGTGGAGGCCGGCGTGA
AGTACGCCAAGGGCGGCCAGAAGTCCTCGGGCTACACCGACACCGGCGTAACCCTGATCTCGGCCAAGCCGGTCAGCGGC
GTGGACAGCAAGGACCTCAAGACGGGCATGGACCTTTGCTGGGGCACCAAATGA

Upstream 100 bases:

>100_bases
AGCGCCACTATAGTTCGATCAAGCCCATCGGTAATGGGTACTTTCCCCGAGGGTAGCCATACCCGACCATGAACATTCCA
TCAAGCAGGAGACAAGTCGC

Downstream 100 bases:

>100_bases
CCCGGCAGTTGCCCGAAGCAGACCTTGGGTTGGCGGGACCGCCTGAGCGCGCCCCTGTTCCTTCAATCACTTCGAGGGGT
TCACCGATGAATTCAACTTC

Product: periplasmic-binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKHTVAHTIARTATLLTLSSLALAATCAFAAGEPVIGLITKTETNPFFVKMKEGAEAEAKKLGAKLLTAAGKSDGDTAGQ
ITALENMAAAGAKTILITASGDAIAPTVKKLQAKGVQVIALDSPFAGADALFATDNYQAGVLVGQYAKAAMAGKPARIVT
LDLLPGHPVGAQRHNGFMKGFGLPAADAKSNELSRDPAVVCMADSFGDRAKSQTAMENCLQKNPDINVVYTINEPAAAGA
FNALKKAGKEKGVLILSVDGGCQGIKDLAAGMITATSQQYPLKMAALGVEAGVKYAKGGQKSSGYTDTGVTLISAKPVSG
VDSKDLKTGMDLCWGTK

Sequences:

>Translated_337_residues
MKHTVAHTIARTATLLTLSSLALAATCAFAAGEPVIGLITKTETNPFFVKMKEGAEAEAKKLGAKLLTAAGKSDGDTAGQ
ITALENMAAAGAKTILITASGDAIAPTVKKLQAKGVQVIALDSPFAGADALFATDNYQAGVLVGQYAKAAMAGKPARIVT
LDLLPGHPVGAQRHNGFMKGFGLPAADAKSNELSRDPAVVCMADSFGDRAKSQTAMENCLQKNPDINVVYTINEPAAAGA
FNALKKAGKEKGVLILSVDGGCQGIKDLAAGMITATSQQYPLKMAALGVEAGVKYAKGGQKSSGYTDTGVTLISAKPVSG
VDSKDLKTGMDLCWGTK
>Mature_337_residues
MKHTVAHTIARTATLLTLSSLALAATCAFAAGEPVIGLITKTETNPFFVKMKEGAEAEAKKLGAKLLTAAGKSDGDTAGQ
ITALENMAAAGAKTILITASGDAIAPTVKKLQAKGVQVIALDSPFAGADALFATDNYQAGVLVGQYAKAAMAGKPARIVT
LDLLPGHPVGAQRHNGFMKGFGLPAADAKSNELSRDPAVVCMADSFGDRAKSQTAMENCLQKNPDINVVYTINEPAAAGA
FNALKKAGKEKGVLILSVDGGCQGIKDLAAGMITATSQQYPLKMAALGVEAGVKYAKGGQKSSGYTDTGVTLISAKPVSG
VDSKDLKTGMDLCWGTK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=302, Percent_Identity=32.7814569536424, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790526, Length=295, Percent_Identity=29.1525423728814, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790674, Length=275, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34444; Mature: 34444

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTVAHTIARTATLLTLSSLALAATCAFAAGEPVIGLITKTETNPFFVKMKEGAEAEAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCHHHH
KLGAKLLTAAGKSDGDTAGQITALENMAAAGAKTILITASGDAIAPTVKKLQAKGVQVIA
HHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCEEEEE
LDSPFAGADALFATDNYQAGVLVGQYAKAAMAGKPARIVTLDLLPGHPVGAQRHNGFMKG
ECCCCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCHHCCCCHHC
FGLPAADAKSNELSRDPAVVCMADSFGDRAKSQTAMENCLQKNPDINVVYTINEPAAAGA
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
FNALKKAGKEKGVLILSVDGGCQGIKDLAAGMITATSQQYPLKMAALGVEAGVKYAKGGQ
HHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCCEEHEEHHHHHCCCCCCCCC
KSSGYTDTGVTLISAKPVSGVDSKDLKTGMDLCWGTK
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKHTVAHTIARTATLLTLSSLALAATCAFAAGEPVIGLITKTETNPFFVKMKEGAEAEAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCHHHH
KLGAKLLTAAGKSDGDTAGQITALENMAAAGAKTILITASGDAIAPTVKKLQAKGVQVIA
HHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCEEEEE
LDSPFAGADALFATDNYQAGVLVGQYAKAAMAGKPARIVTLDLLPGHPVGAQRHNGFMKG
ECCCCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCHHCCCCHHC
FGLPAADAKSNELSRDPAVVCMADSFGDRAKSQTAMENCLQKNPDINVVYTINEPAAAGA
CCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH
FNALKKAGKEKGVLILSVDGGCQGIKDLAAGMITATSQQYPLKMAALGVEAGVKYAKGGQ
HHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCCEEHEEHHHHHCCCCCCCCC
KSSGYTDTGVTLISAKPVSGVDSKDLKTGMDLCWGTK
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]