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The map label for this gene is flhA [H]

Identifier: 91788989

GI number: 91788989

Start: 3304080

End: 3305186

Strand: Direct

Name: flhA [H]

Synonym: Bpro_3129

Alternate gene names: 91788989

Gene position: 3304080-3305186 (Clockwise)

Preceding gene: 91788988

Following gene: 91788990

Centisome position: 63.54

GC content: 61.61

Gene sequence:

>1107_bases
ATGAAAACCAAAGCCGCCGTCGCCTGGAAAGCGGGCCAACCCCTGACCATTGAAACCGTCGATCTTGAAGGCCCGAAGTT
CGGCGAGGTGCTGGTCGAGATCAAGGCCACCGGTATTTGCCACACCGACTACTACACGCTGTCGGGCGCCGATCCGGAAG
GCATCTTCCCGGCCATTCTGGGCCATGAAGGCGCGGGCATCGTGGTCGATGTTGGCCCCGGCGTGACCACGCTCAAGAAG
GGCGACCACGTCATTCCGCTCTACACGCCGGAATGCCGCCAGTGCAAGTTCTGCCTGAGCCGCAAGACCAATCTGTGCCA
GCTGATTCGTGGCACGCAGGGCAAGGGCCTGATGCCCGACGCCACCAGCCGCTTCAGCCTGGACTGCCAACCCATCTTCC
ACTACATGGGCACCAGCACCTTCAGCAACTACACGGTGGCGCCCGGAATTTCGCTGGCCAAAATCCGTGAAGACGCACCG
TTTGACAAGGTCTGCTACATCGGCTGCGGCGTCACCACCGGCATCGGTGCGGTGATCTTCACCGCCAAGGTGGAAGCCGG
CGCCAACGTGGTGATCTTCGGCCTGGGCGGCATTGGCCTGAACGTGATCCAGGGCGCGAAGATGGTGGGCGCCGACAAGA
TCATCGGCGTGGACATCAACCCCGCGCGGCAGGAGATGGCACGCAAATTCGGCATGACGCATTTCATCAACCCCAAGGAA
ACAGACAACGTGGTCGATGCCATCGTGCAGCTGACCGATGGCGGCGCCGACTACAGCTTTGAATGCATAGGCAACACGAA
AGTCATGCGGCAGGCGCTGGAATGCACCCACAAGGGCTGGGGCCGCAGCATCATCATCGGCGTGGCCGAGGCCGGCGCCG
AAATCAGCACCCGCCCCTTCCAGCTGGTCACCGGCCGCAAGTGGGAAGGCTCAGCCTTTGGTGGCGCGCGCGGCCGCACC
GATGTGCCCAAGATCGTCGACTGGTACATGGACGGCAAGATCAACATCGACGACCTGATCACCCACACCATGCCGCTGGA
AGACATCAACAAGGGCTTCGATTTGATGAAGCGTGGCGAGTCGATTCGCGGCGTGGTCATTTACTAG

Upstream 100 bases:

>100_bases
CCAGGGCGCTGCCAGGCAATAGCTGGCTTATAGTGCCGGGTAACCCTTTACCCACCCTCTTTCCCACCTTCTTTCGCGCT
ACGCAGAAGCCATCACCACC

Downstream 100 bases:

>100_bases
CCCCCACGTTCGCTCTACTAGCGTGTAGCTCTCTGCCCCCTCAGGGGGCGCTGCGCCTGCGGTCTGGCAAAGCCAGTCCC
GCGGCCCCTGCTGGTGAAGA

Product: zinc-binding alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAVAWKAGQPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVVDVGPGVTTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDCQPIFHYMGTSTFSNYTVAPGISLAKIREDAP
FDKVCYIGCGVTTGIGAVIFTAKVEAGANVVIFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARKFGMTHFINPKE
TDNVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGSAFGGARGRT
DVPKIVDWYMDGKINIDDLITHTMPLEDINKGFDLMKRGESIRGVVIY

Sequences:

>Translated_368_residues
MKTKAAVAWKAGQPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVVDVGPGVTTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDCQPIFHYMGTSTFSNYTVAPGISLAKIREDAP
FDKVCYIGCGVTTGIGAVIFTAKVEAGANVVIFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARKFGMTHFINPKE
TDNVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGSAFGGARGRT
DVPKIVDWYMDGKINIDDLITHTMPLEDINKGFDLMKRGESIRGVVIY
>Mature_368_residues
MKTKAAVAWKAGQPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVVDVGPGVTTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPDATSRFSLDCQPIFHYMGTSTFSNYTVAPGISLAKIREDAP
FDKVCYIGCGVTTGIGAVIFTAKVEAGANVVIFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARKFGMTHFINPKE
TDNVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGSAFGGARGRT
DVPKIVDWYMDGKINIDDLITHTMPLEDINKGFDLMKRGESIRGVVIY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.4904632152589, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=45.8445040214477, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=44.4444444444444, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=44.3243243243243, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=44.3243243243243, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=43.9024390243902, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=43.9024390243902, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=42.3913043478261, Blast_Score=314, Evalue=9e-86,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=42.6592797783933, Blast_Score=308, Evalue=4e-84,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.8075880758808, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI87082125, Length=385, Percent_Identity=25.7142857142857, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788073, Length=372, Percent_Identity=26.6129032258064, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790045, Length=358, Percent_Identity=28.7709497206704, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081918, Length=367, Percent_Identity=29.1553133514986, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI226510992, Length=374, Percent_Identity=24.5989304812834, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786825, Length=370, Percent_Identity=25.6756756756757, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=24.3553008595989, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788075, Length=378, Percent_Identity=27.5132275132275, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.5231607629428, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.6916890080429, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.4, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.2274052478134, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.3378746594005, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=355, Percent_Identity=26.7605633802817, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=359, Percent_Identity=25.9052924791086, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=374, Percent_Identity=26.2032085561497, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=356, Percent_Identity=25.561797752809, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.6388140161725, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319621, Length=373, Percent_Identity=28.686327077748, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=27.3972602739726, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=25.8760107816712, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.3736263736264, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=28.8409703504043, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=27.8236914600551, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=27.8236914600551, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319258, Length=361, Percent_Identity=26.8698060941828, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=354, Percent_Identity=24.8587570621469, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=59.7826086956522, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17137530, Length=340, Percent_Identity=27.9411764705882, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39583; Mature: 39583

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAVAWKAGQPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGIVVDVGPGVTTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPD
CCCCCEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
ATSRFSLDCQPIFHYMGTSTFSNYTVAPGISLAKIREDAPFDKVCYIGCGVTTGIGAVIF
CCCCEECCHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCCCCEEEEECCHHHCCCEEEE
TAKVEAGANVVIFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARKFGMTHFINPKE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCCC
TDNVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEISTRPF
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRKWEGSAFGGARGRTDVPKIVDWYMDGKINIDDLITHTMPLEDINKGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRGVVIY
CCCEEEEC
>Mature Secondary Structure
MKTKAAVAWKAGQPLTIETVDLEGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGIVVDVGPGVTTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPD
CCCCCEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
ATSRFSLDCQPIFHYMGTSTFSNYTVAPGISLAKIREDAPFDKVCYIGCGVTTGIGAVIF
CCCCEECCHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCCCCEEEEECCHHHCCCEEEE
TAKVEAGANVVIFGLGGIGLNVIQGAKMVGADKIIGVDINPARQEMARKFGMTHFINPKE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCCC
TDNVVDAIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGRSIIIGVAEAGAEISTRPF
HHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGRKWEGSAFGGARGRTDVPKIVDWYMDGKINIDDLITHTMPLEDINKGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHCCHHHHHCCC
SIRGVVIY
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]