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The map label for this gene is eptA [H]

Identifier: 91788990

GI number: 91788990

Start: 3305445

End: 3307139

Strand: Direct

Name: eptA [H]

Synonym: Bpro_3130

Alternate gene names: 91788990

Gene position: 3305445-3307139 (Clockwise)

Preceding gene: 91788989

Following gene: 91788991

Centisome position: 63.56

GC content: 61.83

Gene sequence:

>1695_bases
ATGCCTGCAAACGCCACCCCCTCGCGCGCGGGTATTCGGCCGCTGTGGATCGTCATCCTGTCCAGTCTCTGGATCGCCAC
TGTTTGCAACATCGCGCTGTGGCGTGAGCTGGCGCGCTTGCCCGACCTGAGCAGGGGCGAGGTTCTCACCATCAGCCTGG
CGCTGGCGCTGGTGATCGCGCTGTCGACAGCCGCATTGCTCAGCCTGCTGGCCTGGCGCTGGACCCTGAAGCCGTCCATC
ACGCTCTTTCTGGTGTCTGCCGCCCTGGGCGCCTATTTCATGCTGGCCTATGGCGTGGTCATCGACAAGACCATGATCCT
CAATACCCTGCAGACCGATGTGCGCGAAACGCGCGACCTGCTGAACTGGCACCTGCCGGTAACCATGCTGCTGCTGGCGG
GATTGCCCGGATTTCTCCTGTGGCGGCAAAAAATCCGGATGCAAAGCCCCGCGCGTCAGCTGCTGTCCAACGCCATGACC
CTGGTCACCGCATGCGCCGTGCTGGTGCTCGTTGTGATGCTGTTCTTCCAGAGCATCGCTTCGGTCATGCGCAACTACAC
GCATGTGCGCTACCTGATCAATCCACTCAATTCCTTTTACGCACTGGGAAGGGTTGCGGCCCAGCCCTTTCAGCGCAACG
AATCCATCGTCTTGCCCCTGGGCGAGGACGCAAGGCTCGCTGCCAGCGACGCGGCCGCCGCCAAACCGCCTCTGTTGCTG
CTGGTGCTGGGTGAAACCGCACGCAGCGGCAATTTCGCGATCAATGGCTATCGGCGGGCCACCACACCGGAGCTGGCGCG
GGAAAACATTGCCAGCCAGCGCAACGCCTGGTCCTGCGGCACCAGCACGGCGGTGTCGGTGCCCTGCATGTTTTCCAATT
TCGGCCGCGAAGCGTATGACTCACGGCCTGCGAATTACGAAGGCTTGCTCGATGTTTTGCAGCACTCAGGCCTGGCCGTG
GTCTGGATAGACAACCAGTCGGGCTGCAAAGGGGTATGCGACCGGGTCGCCAGTGTGTACACCACCAGCCTGAAGGTACC
TGGCCTGTGCGACGGCGGGGAATGCTTTGATGAGGTGATGCTGCAAAACATGGAGGAACGCATCGCTGCCCTGCCTGCAG
AACGCAGGGCCAGGGGTGTGGTCGTAGTGATGCACCAGATCGGCGGCCACGGCCCGGCTTACTACCAGCGTTCACCACCG
GCCTTCAAGAAGTTCTTGCCCGAATGCATCAACAACGCCCTGCAAAGCTGCGGACGCGATGAACTGATGAATGCCTATGA
CAACAGCGTTGTCTACGCCGACCATCTGCTGGGGTCCACCATTCGGTGGCTCAAAACGCAGGAGGCGCACAACTCGCCCG
CCATGCTGTATGTGGCCGACCACGGCGAGTCCCTGGGCGAAAACAACCTTTACCTGCATGGCATGCCCTACGGGATGGCG
CCCGACGTGCAAAAGCGCGTACCCTGGATCACCTGGCTGTCGCCGGAATTCGAGCAACTCAGCAGTATCACCACGGCCTG
CCTCAAGCAGCAGCTCGATGCGCCGGTGAGCCATGACAATTACTTTCACTCGGTGCTGGGCCTGATGAAGGTGCAGACCA
GCGTCTACCAGCCCGCGCTCGATATCTATGCCGGCTGCGCCAACCGTGGTCCCGGCAGTGTGCACGCGCAGACGTTGCGC
ACTGACTCGTCCTGA

Upstream 100 bases:

>100_bases
TTAAGCGTCTCTTAAGTCGCCCAAAATACACTGCGGCGCTATGCCGCTGCTCTCCCTCCCCTCAGGCTCTGCGTCCGCGT
TCAGTCCGGTGTCCAGGTCA

Downstream 100 bases:

>100_bases
CGAATCGTCCTGACGCCGCATAGGGTTTTTAGGCAGCTGCAGCCACTTGTTGCACGTCGGTTTAATATTCACTTCCACCT
CAACTACAAATACATTATTC

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MPANATPSRAGIRPLWIVILSSLWIATVCNIALWRELARLPDLSRGEVLTISLALALVIALSTAALLSLLAWRWTLKPSI
TLFLVSAALGAYFMLAYGVVIDKTMILNTLQTDVRETRDLLNWHLPVTMLLLAGLPGFLLWRQKIRMQSPARQLLSNAMT
LVTACAVLVLVVMLFFQSIASVMRNYTHVRYLINPLNSFYALGRVAAQPFQRNESIVLPLGEDARLAASDAAAAKPPLLL
LVLGETARSGNFAINGYRRATTPELARENIASQRNAWSCGTSTAVSVPCMFSNFGREAYDSRPANYEGLLDVLQHSGLAV
VWIDNQSGCKGVCDRVASVYTTSLKVPGLCDGGECFDEVMLQNMEERIAALPAERRARGVVVVMHQIGGHGPAYYQRSPP
AFKKFLPECINNALQSCGRDELMNAYDNSVVYADHLLGSTIRWLKTQEAHNSPAMLYVADHGESLGENNLYLHGMPYGMA
PDVQKRVPWITWLSPEFEQLSSITTACLKQQLDAPVSHDNYFHSVLGLMKVQTSVYQPALDIYAGCANRGPGSVHAQTLR
TDSS

Sequences:

>Translated_564_residues
MPANATPSRAGIRPLWIVILSSLWIATVCNIALWRELARLPDLSRGEVLTISLALALVIALSTAALLSLLAWRWTLKPSI
TLFLVSAALGAYFMLAYGVVIDKTMILNTLQTDVRETRDLLNWHLPVTMLLLAGLPGFLLWRQKIRMQSPARQLLSNAMT
LVTACAVLVLVVMLFFQSIASVMRNYTHVRYLINPLNSFYALGRVAAQPFQRNESIVLPLGEDARLAASDAAAAKPPLLL
LVLGETARSGNFAINGYRRATTPELARENIASQRNAWSCGTSTAVSVPCMFSNFGREAYDSRPANYEGLLDVLQHSGLAV
VWIDNQSGCKGVCDRVASVYTTSLKVPGLCDGGECFDEVMLQNMEERIAALPAERRARGVVVVMHQIGGHGPAYYQRSPP
AFKKFLPECINNALQSCGRDELMNAYDNSVVYADHLLGSTIRWLKTQEAHNSPAMLYVADHGESLGENNLYLHGMPYGMA
PDVQKRVPWITWLSPEFEQLSSITTACLKQQLDAPVSHDNYFHSVLGLMKVQTSVYQPALDIYAGCANRGPGSVHAQTLR
TDSS
>Mature_563_residues
PANATPSRAGIRPLWIVILSSLWIATVCNIALWRELARLPDLSRGEVLTISLALALVIALSTAALLSLLAWRWTLKPSIT
LFLVSAALGAYFMLAYGVVIDKTMILNTLQTDVRETRDLLNWHLPVTMLLLAGLPGFLLWRQKIRMQSPARQLLSNAMTL
VTACAVLVLVVMLFFQSIASVMRNYTHVRYLINPLNSFYALGRVAAQPFQRNESIVLPLGEDARLAASDAAAAKPPLLLL
VLGETARSGNFAINGYRRATTPELARENIASQRNAWSCGTSTAVSVPCMFSNFGREAYDSRPANYEGLLDVLQHSGLAVV
WIDNQSGCKGVCDRVASVYTTSLKVPGLCDGGECFDEVMLQNMEERIAALPAERRARGVVVVMHQIGGHGPAYYQRSPPA
FKKFLPECINNALQSCGRDELMNAYDNSVVYADHLLGSTIRWLKTQEAHNSPAMLYVADHGESLGENNLYLHGMPYGMAP
DVQKRVPWITWLSPEFEQLSSITTACLKQQLDAPVSHDNYFHSVLGLMKVQTSVYQPALDIYAGCANRGPGSVHAQTLRT
DSS

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=537, Percent_Identity=37.4301675977654, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87082286, Length=480, Percent_Identity=26.0416666666667, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87082223, Length=264, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61994; Mature: 61863

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANATPSRAGIRPLWIVILSSLWIATVCNIALWRELARLPDLSRGEVLTISLALALVIA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LSTAALLSLLAWRWTLKPSITLFLVSAALGAYFMLAYGVVIDKTMILNTLQTDVRETRDL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNWHLPVTMLLLAGLPGFLLWRQKIRMQSPARQLLSNAMTLVTACAVLVLVVMLFFQSIA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVMRNYTHVRYLINPLNSFYALGRVAAQPFQRNESIVLPLGEDARLAASDAAAAKPPLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
LVLGETARSGNFAINGYRRATTPELARENIASQRNAWSCGTSTAVSVPCMFSNFGREAYD
EEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCHHHHC
SRPANYEGLLDVLQHSGLAVVWIDNQSGCKGVCDRVASVYTTSLKVPGLCDGGECFDEVM
CCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LQNMEERIAALPAERRARGVVVVMHQIGGHGPAYYQRSPPAFKKFLPECINNALQSCGRD
HHHHHHHHHHCCHHHHCCCEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
ELMNAYDNSVVYADHLLGSTIRWLKTQEAHNSPAMLYVADHGESLGENNLYLHGMPYGMA
HHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCC
PDVQKRVPWITWLSPEFEQLSSITTACLKQQLDAPVSHDNYFHSVLGLMKVQTSVYQPAL
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DIYAGCANRGPGSVHAQTLRTDSS
HHHHCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
PANATPSRAGIRPLWIVILSSLWIATVCNIALWRELARLPDLSRGEVLTISLALALVIA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LSTAALLSLLAWRWTLKPSITLFLVSAALGAYFMLAYGVVIDKTMILNTLQTDVRETRDL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNWHLPVTMLLLAGLPGFLLWRQKIRMQSPARQLLSNAMTLVTACAVLVLVVMLFFQSIA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVMRNYTHVRYLINPLNSFYALGRVAAQPFQRNESIVLPLGEDARLAASDAAAAKPPLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
LVLGETARSGNFAINGYRRATTPELARENIASQRNAWSCGTSTAVSVPCMFSNFGREAYD
EEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCHHHHC
SRPANYEGLLDVLQHSGLAVVWIDNQSGCKGVCDRVASVYTTSLKVPGLCDGGECFDEVM
CCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LQNMEERIAALPAERRARGVVVVMHQIGGHGPAYYQRSPPAFKKFLPECINNALQSCGRD
HHHHHHHHHHCCHHHHCCCEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
ELMNAYDNSVVYADHLLGSTIRWLKTQEAHNSPAMLYVADHGESLGENNLYLHGMPYGMA
HHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCC
PDVQKRVPWITWLSPEFEQLSSITTACLKQQLDAPVSHDNYFHSVLGLMKVQTSVYQPAL
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DIYAGCANRGPGSVHAQTLRTDSS
HHHHCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]