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The map label for this gene is cbiH [H]

Identifier: 91788629

GI number: 91788629

Start: 2909581

End: 2910576

Strand: Reverse

Name: cbiH [H]

Synonym: Bpro_2767

Alternate gene names: 91788629

Gene position: 2910576-2909581 (Counterclockwise)

Preceding gene: 91788630

Following gene: 91788628

Centisome position: 55.97

GC content: 64.56

Gene sequence:

>996_bases
ATGACCGGCCCCAAAGGAAAAATCATGCTGGTGGGCATTGGTCCCGGCAGCCATGACCATATGACGCAGCGCGCCCGTGC
CGCCATTGCCGAGGCGGACGTGGTGGTGGGCTACGTCACCTACATCAAGCTGGTGGCCGATTTGCTGGAAGGCAAAGAGG
TCGTGCGCAAAGGCATGACTGAAGAGCTGGACCGCGCGGTGCACGCGTTGGCCAGCGCCCGCGAAGGCAAGAAGGTTGCG
CTCATCTCCAGTGGCGACGCTGGGGTGTATGGCATGGCCGGGCCCACCTATGAGGTGCTGTTCCAGGCCGGCTGGACGCC
CGAGTCCGACGTGCAGGTGGAAGTCGTGCCGGGGGCCTCGGCCATCAACGCCTGTGCGGCGCTGGTGGGCGCGCCCCTGA
CGCACGACTTCTGCTCGATCTCCCTGTCTGACCTGCTCACGCCCTGGCCGGTGATTGCACGCCGGCTGGATGCGGTGGCG
GCATCGGATTTTGTGGTGGCGCTGTACAACCCCAAGAGCGGCCGCCGTACCCAGCAAATCGTGCAGGCACAGCAGCTGTT
CTTGCGGCACCGCAGGCCCGACACGCCGGTGGCGGTGGTCAAGTCGGCTTACCGCAGACGCGAGCGCATCGAGTTCACCA
CACTGGACAAGATGAGCGACTGCGACATCGGCATGCTCACCACGGTACTGATCGGCAACAGCCACACCTTTGTACGGCAT
GGCCTGATGGTTACGCCGCGCGGCTACGCCAACAAGTACGAGCTGCACGGCGATGGCAGCACGCGCGAGGGCGAAAGGCC
TGGCCGTTCGCTGTCCACCGGCCTGCTGGGCTGGATGGAAAACCTGCGCATTGACCATGCTGAAGGCGCGAGCGTTGCTG
CGCTGGCGTTGCGCCACAAGCTGCCTGCGGACTACATCGAGGCAGTGCTGACCGCGCCGATTGAAGAAGAGGCCGTCAAG
GAAGAAACGGCCAGTGCCGAGGAATCGGAAGCATGA

Upstream 100 bases:

>100_bases
TGGTATGCGGCGCGGCGTTTCAGCGCTGGCTGCGTCCGGTGCTGGAAGCAACTTTTGAACGCTCGAAGCCGGTTGCATCA
GCCGGCACGGCGGTGAGCCC

Downstream 100 bases:

>100_bases
GCGAAGCGGTGGTCAAACCCAAAATTGGCGCTTACAAACGCCATTTGCTGATCTGCACCGGCCCGCGCTGTACGCAGGAT
GGTGCGTCGCAGGAGCTGTT

Product: precorrin-3 methyltransferase/precorrin-6Y C5,15-methyltransferase

Products: 2S-adenosyl-L-homocysteine; precorrin; sirohydrochlorin; NADH; Proton; siroheme [C]

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTGPKGKIMLVGIGPGSHDHMTQRARAAIAEADVVVGYVTYIKLVADLLEGKEVVRKGMTEELDRAVHALASAREGKKVA
LISSGDAGVYGMAGPTYEVLFQAGWTPESDVQVEVVPGASAINACAALVGAPLTHDFCSISLSDLLTPWPVIARRLDAVA
ASDFVVALYNPKSGRRTQQIVQAQQLFLRHRRPDTPVAVVKSAYRRRERIEFTTLDKMSDCDIGMLTTVLIGNSHTFVRH
GLMVTPRGYANKYELHGDGSTREGERPGRSLSTGLLGWMENLRIDHAEGASVAALALRHKLPADYIEAVLTAPIEEEAVK
EETASAEESEA

Sequences:

>Translated_331_residues
MTGPKGKIMLVGIGPGSHDHMTQRARAAIAEADVVVGYVTYIKLVADLLEGKEVVRKGMTEELDRAVHALASAREGKKVA
LISSGDAGVYGMAGPTYEVLFQAGWTPESDVQVEVVPGASAINACAALVGAPLTHDFCSISLSDLLTPWPVIARRLDAVA
ASDFVVALYNPKSGRRTQQIVQAQQLFLRHRRPDTPVAVVKSAYRRRERIEFTTLDKMSDCDIGMLTTVLIGNSHTFVRH
GLMVTPRGYANKYELHGDGSTREGERPGRSLSTGLLGWMENLRIDHAEGASVAALALRHKLPADYIEAVLTAPIEEEAVK
EETASAEESEA
>Mature_330_residues
TGPKGKIMLVGIGPGSHDHMTQRARAAIAEADVVVGYVTYIKLVADLLEGKEVVRKGMTEELDRAVHALASAREGKKVAL
ISSGDAGVYGMAGPTYEVLFQAGWTPESDVQVEVVPGASAINACAALVGAPLTHDFCSISLSDLLTPWPVIARRLDAVAA
SDFVVALYNPKSGRRTQQIVQAQQLFLRHRRPDTPVAVVKSAYRRRERIEFTTLDKMSDCDIGMLTTVLIGNSHTFVRHG
LMVTPRGYANKYELHGDGSTREGERPGRSLSTGLLGWMENLRIDHAEGASVAALALRHKLPADYIEAVLTAPIEEEAVKE
ETASAEESEA

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=270, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.107; 1.3.1.76; 4.99.1.4 [C]

Molecular weight: Translated: 35726; Mature: 35595

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPKGKIMLVGIGPGSHDHMTQRARAAIAEADVVVGYVTYIKLVADLLEGKEVVRKGMT
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EELDRAVHALASAREGKKVALISSGDAGVYGMAGPTYEVLFQAGWTPESDVQVEVVPGAS
HHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHEECCCCCCCCCEEEEECCCH
AINACAALVGAPLTHDFCSISLSDLLTPWPVIARRLDAVAASDFVVALYNPKSGRRTQQI
HHHHHHHHHCCCCCHHHHHCCHHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
VQAQQLFLRHRRPDTPVAVVKSAYRRRERIEFTTLDKMSDCDIGMLTTVLIGNSHTFVRH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHC
GLMVTPRGYANKYELHGDGSTREGERPGRSLSTGLLGWMENLRIDHAEGASVAALALRHK
CEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
LPADYIEAVLTAPIEEEAVKEETASAEESEA
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGPKGKIMLVGIGPGSHDHMTQRARAAIAEADVVVGYVTYIKLVADLLEGKEVVRKGMT
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EELDRAVHALASAREGKKVALISSGDAGVYGMAGPTYEVLFQAGWTPESDVQVEVVPGAS
HHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHEECCCCCCCCCEEEEECCCH
AINACAALVGAPLTHDFCSISLSDLLTPWPVIARRLDAVAASDFVVALYNPKSGRRTQQI
HHHHHHHHHCCCCCHHHHHCCHHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
VQAQQLFLRHRRPDTPVAVVKSAYRRRERIEFTTLDKMSDCDIGMLTTVLIGNSHTFVRH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHC
GLMVTPRGYANKYELHGDGSTREGERPGRSLSTGLLGWMENLRIDHAEGASVAALALRHK
CEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
LPADYIEAVLTAPIEEEAVKEETASAEESEA
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2S-Adenosyl-L-methionine; uroporphyrin III; dihydrosirohydrochlorin; NAD; sirohydrochlorin; Fe (II) [C]

Specific reaction: 2S-Adenosyl-L-methionine + uroporphyrin III = 2S-adenosyl-L-homocysteine + precorrin dihydrosirohydrochlorin + NAD = sirohydrochlorin + NADH sirohydrochlorin + Fe (II) = 2 Proton + siroheme [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]