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The map label for this gene is ywfA [H]

Identifier: 91788197

GI number: 91788197

Start: 2424032

End: 2425252

Strand: Reverse

Name: ywfA [H]

Synonym: Bpro_2328

Alternate gene names: 91788197

Gene position: 2425252-2424032 (Counterclockwise)

Preceding gene: 91788198

Following gene: 91788196

Centisome position: 46.64

GC content: 65.27

Gene sequence:

>1221_bases
ATGACCACCGGTTCCGCCACCCTCTCCGCGGCTACGCGACGTTCCGTCCTGCTGCTGTCTTTTGCCACCTTCGCCAGCAT
GGCGGCACAGCGTATTTGCGACGCGATGCTCCCCGAGCTTTCACGTACTTTTTCCGTGAGCCTGGCGCAGGCGGCGCAGG
TGGTGTCGGTGTTTGCGGTGGTGTACGGCCTGGCACAGCTGTTTTATGGCCCGCTGGGCGACCGGCGGAGCAAGTTCGGC
ATCGTCACTTTTGCCACGCTGTCGTGTTGCGCTGGCAGCCTCATGTCGGCTTTTGCTGTCAGCCTGGACATGCTGGTGCT
GGCGCGTGTCATGGTGGCGCTGGGCGCGGCGGCCATTATTCCGCTGTCCATGGCATGGATCGGCGACACCGTCGCCTACG
AGCTGCGCCAGGAAACGCTGGCACGCGTCGGCCTGGGCACCACGCTGGGTATTGTGTGTGGCCAGCTGGTGGGCGGCTTG
TTGAGCGATACGCTGGGCTGGCGCTGGGCGTTCGGACTCATGACGTTGCTGTTCGGCGTGGTGGGATCGCTTCTCTTCAT
CGACCGGCGCCGCCAGCAGGCCGAACGCCTGCCAACCACCAACACCGAACCCGTCGTGCGGCCCGGTTTTGTGGCGCAGG
CCCTGATCATCATCACCGGACCGTGGTCGCGCATCGTGCTCGGGGTCGCTTTTGTCGAAGGCGCGGCAGGCTTCGGCGTG
CTGGCCATCTGGGCGTCGCATCTGCACCAGGCGCTGGGCCTGTCGCTGTCGGCAGCTGGTGCCATTGTGGCGCTGTTCGG
CCTGGGCGGCATGCTTTACATGGTGGTGGCGCGCCAGTTGATTCGGCGCTTGGGTGAACAGGGTTTGACCCTGCTGGGGG
GCAGCCTGATGGGGCTGTCTGCGCTAGTGCTGGGCTTCACGCCGCACTGGTTACTCAGCCTTCCGGCATGCCTGCTGGCC
GGTTTTGGCTTCTTCATGTTCCACAACACCATGCAGACCAATGCCACGCAGATGGCGCCTAAGGCACGCGGCACAGGCGT
GTCCCTGTTCGCCTCGGCACTGTTCATGGGCCAGTCGGTGGGCGTGCTGCTGGCCGCCGGCCTGGTGGACCGCATCGGCT
CCGGCGCCGTGATTGCGCTCGGAGGCGGCGTGTTGGTGAGCATTGGTATTTTCTTCGCGCGGGCATTGCGCCGGCGTGAG
GAGTTGATGCGCCTGCAGTGA

Upstream 100 bases:

>100_bases
GGGTCAGCGCTGCCTACGCTGCCTACAATCGACCGGCACAGACGTCAGGCTCCAGCCCGTACTGAACTGGCACACCCGAT
CAGCCTGACTTCATCCATCC

Downstream 100 bases:

>100_bases
GTGCTGGAGGACATTCGCTGCGCCACTTGGGATAGATTGGTATGCCCGTTTTTTTAACGCAAAATCCAAATCCTGTTGCC
CCCGTCACCTCGGCTTGAGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTTGSATLSAATRRSVLLLSFATFASMAAQRICDAMLPELSRTFSVSLAQAAQVVSVFAVVYGLAQLFYGPLGDRRSKFG
IVTFATLSCCAGSLMSAFAVSLDMLVLARVMVALGAAAIIPLSMAWIGDTVAYELRQETLARVGLGTTLGIVCGQLVGGL
LSDTLGWRWAFGLMTLLFGVVGSLLFIDRRRQQAERLPTTNTEPVVRPGFVAQALIIITGPWSRIVLGVAFVEGAAGFGV
LAIWASHLHQALGLSLSAAGAIVALFGLGGMLYMVVARQLIRRLGEQGLTLLGGSLMGLSALVLGFTPHWLLSLPACLLA
GFGFFMFHNTMQTNATQMAPKARGTGVSLFASALFMGQSVGVLLAAGLVDRIGSGAVIALGGGVLVSIGIFFARALRRRE
ELMRLQ

Sequences:

>Translated_406_residues
MTTGSATLSAATRRSVLLLSFATFASMAAQRICDAMLPELSRTFSVSLAQAAQVVSVFAVVYGLAQLFYGPLGDRRSKFG
IVTFATLSCCAGSLMSAFAVSLDMLVLARVMVALGAAAIIPLSMAWIGDTVAYELRQETLARVGLGTTLGIVCGQLVGGL
LSDTLGWRWAFGLMTLLFGVVGSLLFIDRRRQQAERLPTTNTEPVVRPGFVAQALIIITGPWSRIVLGVAFVEGAAGFGV
LAIWASHLHQALGLSLSAAGAIVALFGLGGMLYMVVARQLIRRLGEQGLTLLGGSLMGLSALVLGFTPHWLLSLPACLLA
GFGFFMFHNTMQTNATQMAPKARGTGVSLFASALFMGQSVGVLLAAGLVDRIGSGAVIALGGGVLVSIGIFFARALRRRE
ELMRLQ
>Mature_405_residues
TTGSATLSAATRRSVLLLSFATFASMAAQRICDAMLPELSRTFSVSLAQAAQVVSVFAVVYGLAQLFYGPLGDRRSKFGI
VTFATLSCCAGSLMSAFAVSLDMLVLARVMVALGAAAIIPLSMAWIGDTVAYELRQETLARVGLGTTLGIVCGQLVGGLL
SDTLGWRWAFGLMTLLFGVVGSLLFIDRRRQQAERLPTTNTEPVVRPGFVAQALIIITGPWSRIVLGVAFVEGAAGFGVL
AIWASHLHQALGLSLSAAGAIVALFGLGGMLYMVVARQLIRRLGEQGLTLLGGSLMGLSALVLGFTPHWLLSLPACLLAG
FGFFMFHNTMQTNATQMAPKARGTGVSLFASALFMGQSVGVLLAAGLVDRIGSGAVIALGGGVLVSIGIFFARALRRREE
LMRLQ

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=153, Percent_Identity=26.797385620915, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42622; Mature: 42491

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTGSATLSAATRRSVLLLSFATFASMAAQRICDAMLPELSRTFSVSLAQAAQVVSVFAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGLAQLFYGPLGDRRSKFGIVTFATLSCCAGSLMSAFAVSLDMLVLARVMVALGAAAII
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLSMAWIGDTVAYELRQETLARVGLGTTLGIVCGQLVGGLLSDTLGWRWAFGLMTLLFGV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSLLFIDRRRQQAERLPTTNTEPVVRPGFVAQALIIITGPWSRIVLGVAFVEGAAGFGV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEHHEEECCHHHHHHHHHHHHCCCCHHH
LAIWASHLHQALGLSLSAAGAIVALFGLGGMLYMVVARQLIRRLGEQGLTLLGGSLMGLS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALVLGFTPHWLLSLPACLLAGFGFFMFHNTMQTNATQMAPKARGTGVSLFASALFMGQSV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHCCHH
GVLLAAGLVDRIGSGAVIALGGGVLVSIGIFFARALRRREELMRLQ
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]