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The map label for this gene is aat

Identifier: 91788195

GI number: 91788195

Start: 2421239

End: 2422006

Strand: Reverse

Name: aat

Synonym: Bpro_2326

Alternate gene names: 91788195

Gene position: 2422006-2421239 (Counterclockwise)

Preceding gene: 91788196

Following gene: 91788194

Centisome position: 46.57

GC content: 57.16

Gene sequence:

>768_bases
ATGGGAAAACTGCCGCGCCCCGTACCCTGGCTTGCCGCCGGGGAGAATTTTCCTCCGCTGGAATTCGCTTGGGGGCGTCA
CGACCCTGCCCCCGGTCTGTTAGCCGCTGGCGCGGCGCTTGATGTGCCAACGTTGATTCAAGCTTATTCGCGCGGAATTT
TCCCCTGGTTTAGTCTGGGCCAGCCGGTGCTCTGGTGGAGCCCAGACCCTCGGATGGTGCTGCATACCGAAAAATTCAAG
CTGCATAGATCCCTGCGCAAAAGCCTGATTCATTTTCTGGACGATCCGGAATGCGAAATAAAGTTTGACAGTGCCTTTGA
GCATGTCATCAAAGCCTGCGCCAGCCAACCCCGTGCGGGCCAGCCGGGCACCTGGATTGTTCCGGATATGGTGCAAGCCT
ACAGCAGCCTGCATCGGGCCGGGTATGCCCACAGTGTAGAAACATGGGTGCGCGGCAAGCTGGTAGGAGGTCTGTATTGC
GTGAAATTGGGACGAATGGTATTTGGCGAATCCATGTTCGCCCATCAGACCGACGCCTCCAAGATTGCATTGGCTGCGCT
TGTGGGTTTTTGCCGTGCCCACCAGATCGCCATGATTGACTGCCAGCAAAACACCCGCCATTTGGCCTCGCTCGGCGCGG
CTGAAATTGATCGCGCGGACTTCGTGAGGCATCTGGCTCAAAACGTGGAGAAAACCACTCCCCCCTGGCAATTTGAACCC
GTATACTGGAAACAGATACTGACGGCTGAAAAGCCGACGCTACCGTGA

Upstream 100 bases:

>100_bases
CAATCCACCGCGCCCACTCAAAGCTGCCAGCTCAGCTACCATCTGTGGATGTCTCTCCTGAAACATTTTTAAAGCTCTGA
TGTTGCCAAGATTTTGAGCC

Downstream 100 bases:

>100_bases
CGCACCTCAAAGATCTGCCTCTGCAAACCTTGCAGTTTTATGCAACAGCGCCCTATCCCTGCAGCTATCTGCCGGGGAAG
CAGGCCCGCTCTCAGGTTGC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MGKLPRPVPWLAAGENFPPLEFAWGRHDPAPGLLAAGAALDVPTLIQAYSRGIFPWFSLGQPVLWWSPDPRMVLHTEKFK
LHRSLRKSLIHFLDDPECEIKFDSAFEHVIKACASQPRAGQPGTWIVPDMVQAYSSLHRAGYAHSVETWVRGKLVGGLYC
VKLGRMVFGESMFAHQTDASKIALAALVGFCRAHQIAMIDCQQNTRHLASLGAAEIDRADFVRHLAQNVEKTTPPWQFEP
VYWKQILTAEKPTLP

Sequences:

>Translated_255_residues
MGKLPRPVPWLAAGENFPPLEFAWGRHDPAPGLLAAGAALDVPTLIQAYSRGIFPWFSLGQPVLWWSPDPRMVLHTEKFK
LHRSLRKSLIHFLDDPECEIKFDSAFEHVIKACASQPRAGQPGTWIVPDMVQAYSSLHRAGYAHSVETWVRGKLVGGLYC
VKLGRMVFGESMFAHQTDASKIALAALVGFCRAHQIAMIDCQQNTRHLASLGAAEIDRADFVRHLAQNVEKTTPPWQFEP
VYWKQILTAEKPTLP
>Mature_254_residues
GKLPRPVPWLAAGENFPPLEFAWGRHDPAPGLLAAGAALDVPTLIQAYSRGIFPWFSLGQPVLWWSPDPRMVLHTEKFKL
HRSLRKSLIHFLDDPECEIKFDSAFEHVIKACASQPRAGQPGTWIVPDMVQAYSSLHRAGYAHSVETWVRGKLVGGLYCV
KLGRMVFGESMFAHQTDASKIALAALVGFCRAHQIAMIDCQQNTRHLASLGAAEIDRADFVRHLAQNVEKTTPPWQFEPV
YWKQILTAEKPTLP

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=196, Percent_Identity=45.4081632653061, Blast_Score=165, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_POLSJ (Q12B43)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549147.1
- ProteinModelPortal:   Q12B43
- SMR:   Q12B43
- STRING:   Q12B43
- GeneID:   4015016
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2326
- NMPDR:   fig|296591.1.peg.3750
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q12B43
- ProtClustDB:   PRK00301
- BioCyc:   PSP296591:BPRO_2326-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 28528; Mature: 28397

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLPRPVPWLAAGENFPPLEFAWGRHDPAPGLLAAGAALDVPTLIQAYSRGIFPWFSLG
CCCCCCCCCCEECCCCCCCCCEECCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCHHHCC
QPVLWWSPDPRMVLHTEKFKLHRSLRKSLIHFLDDPECEIKFDSAFEHVIKACASQPRAG
CCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCC
QPGTWIVPDMVQAYSSLHRAGYAHSVETWVRGKLVGGLYCVKLGRMVFGESMFAHQTDAS
CCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHH
KIALAALVGFCRAHQIAMIDCQQNTRHLASLGAAEIDRADFVRHLAQNVEKTTPPWQFEP
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHCCCCCCCCH
VYWKQILTAEKPTLP
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKLPRPVPWLAAGENFPPLEFAWGRHDPAPGLLAAGAALDVPTLIQAYSRGIFPWFSLG
CCCCCCCCCEECCCCCCCCCEECCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCHHHCC
QPVLWWSPDPRMVLHTEKFKLHRSLRKSLIHFLDDPECEIKFDSAFEHVIKACASQPRAG
CCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCC
QPGTWIVPDMVQAYSSLHRAGYAHSVETWVRGKLVGGLYCVKLGRMVFGESMFAHQTDAS
CCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHH
KIALAALVGFCRAHQIAMIDCQQNTRHLASLGAAEIDRADFVRHLAQNVEKTTPPWQFEP
HHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHCCCCCCCCH
VYWKQILTAEKPTLP
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA