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The map label for this gene is pilT [H]

Identifier: 91787871

GI number: 91787871

Start: 2062621

End: 2063826

Strand: Reverse

Name: pilT [H]

Synonym: Bpro_1995

Alternate gene names: 91787871

Gene position: 2063826-2062621 (Counterclockwise)

Preceding gene: 91787872

Following gene: 91787870

Centisome position: 39.69

GC content: 60.78

Gene sequence:

>1206_bases
ATGACTGAAGGCAGGACTATGAGCACGATGGAGAGGATCCTTCGTTTGATGGCGGATAAAAAAGCATCGGATGTTTACCT
GTCGGCTCATTCGCCCGCCCTCATCAAGATCAACGGCCAGGCCATTCCCATCAATGCCCAGATCCTGCCGCCCGACGCGC
CGCGCAACCTGCTGGCCGAAGTGCTGCCGCCCGAGCGGATCGAAGAACTCGAGGAAGTGGGCGAGCTCAACATGGCGCTG
CCGATTGCCGGCCTGGGCAATTTCCGCATCAGCGGCTTCCGGCAGCGCAGCACCTATGCGGCGGTGATCCGCTTCATTCC
GAACGAAATCCCTGCACTGGACAGCCTGAACGTGCCGGCCATCCTGTCCAGCCTGATCATGGAAAAACGCGGCCTGCTGC
TGATGGTGGGCTCCACCGGCGCGGGAAAGAGCACCACGCTGGCAGCCATGATGGACCACCGCAACGAAACCACGCTGGGC
CACATCCTGACCATTGAAGACCCGGTTGAATTCCTGTTCACCAACAAGAAATCGGTGGTCAACCAGCGCGAGGTGGGCAG
TGACACCCAATCCCTGCAGGTGGCACTGCGAAACGCGCTACGCCAGGCGCCTGATGTGATTCTGATCGGTGAAATCCGCG
ACCGCGAAACCATGTCGTCCGCCATTGCCTATGCGCAGTCGGGCCATTTGTGCCTGGCGACCATGCACGCCAACAACAGC
TACCAGGCGCTGAACCGCATCCTGAGCTTTTACCCGGTGGAGGTTCGCCCCACCATGCTGGGCGACCTGGCCGCAGCCCT
GAAGGCGATCGTGTCGCAGCGGCTGCTGCGTACCCAGGACGGCAACCGCTCGCCGGCAGTGGAAGTCATGCTCAACACCA
AGCTGGTATCCGAGCTGATCGAAAAAGGCGACTTCTCGGGGGTCAAGGAGGCCATGGAGAAATCCATGGCCGAGGGCTCG
CAAACCTTTGAACAGGACATCGCCCGGATGATCGTGGAAGGCACCGTTACCCGCAAGGAAGGCATTTTGCATGCGGATTC
GCCAACCAACCTGATGTGGCGCCTTGAGAACGACTTTGCCGGGGCTGCCAAGGACAAGGCGAAGGAGGCCGAAGAAGAGC
CGGATGACGAGGCCTCGTTCACGGAGATCACCCTCGACGTTCACCACGGCTCGAAATACGGCACCACGGGCTTCGGCTCG
CTCTGA

Upstream 100 bases:

>100_bases
GTGATCGTCAAGCGGGTGGATGCCAAGACGCGCGCCACGACCAGCCTGAACGACCTGCTGCGTGATTGATTTGACTGAAG
ACCTGAAAGGATAATTGATC

Downstream 100 bases:

>100_bases
GCGCGGGCCCGTCAACCTTTTCTATGTCAGCTACCCTTCGCCTCACCGAACAACTGATTTCCCGGCCGTCTGTCACACCG
CTCGACGAGGGCTGCATCGA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTEGRTMSTMERILRLMADKKASDVYLSAHSPALIKINGQAIPINAQILPPDAPRNLLAEVLPPERIEELEEVGELNMAL
PIAGLGNFRISGFRQRSTYAAVIRFIPNEIPALDSLNVPAILSSLIMEKRGLLLMVGSTGAGKSTTLAAMMDHRNETTLG
HILTIEDPVEFLFTNKKSVVNQREVGSDTQSLQVALRNALRQAPDVILIGEIRDRETMSSAIAYAQSGHLCLATMHANNS
YQALNRILSFYPVEVRPTMLGDLAAALKAIVSQRLLRTQDGNRSPAVEVMLNTKLVSELIEKGDFSGVKEAMEKSMAEGS
QTFEQDIARMIVEGTVTRKEGILHADSPTNLMWRLENDFAGAAKDKAKEAEEEPDDEASFTEITLDVHHGSKYGTTGFGS
L

Sequences:

>Translated_401_residues
MTEGRTMSTMERILRLMADKKASDVYLSAHSPALIKINGQAIPINAQILPPDAPRNLLAEVLPPERIEELEEVGELNMAL
PIAGLGNFRISGFRQRSTYAAVIRFIPNEIPALDSLNVPAILSSLIMEKRGLLLMVGSTGAGKSTTLAAMMDHRNETTLG
HILTIEDPVEFLFTNKKSVVNQREVGSDTQSLQVALRNALRQAPDVILIGEIRDRETMSSAIAYAQSGHLCLATMHANNS
YQALNRILSFYPVEVRPTMLGDLAAALKAIVSQRLLRTQDGNRSPAVEVMLNTKLVSELIEKGDFSGVKEAMEKSMAEGS
QTFEQDIARMIVEGTVTRKEGILHADSPTNLMWRLENDFAGAAKDKAKEAEEEPDDEASFTEITLDVHHGSKYGTTGFGS
L
>Mature_400_residues
TEGRTMSTMERILRLMADKKASDVYLSAHSPALIKINGQAIPINAQILPPDAPRNLLAEVLPPERIEELEEVGELNMALP
IAGLGNFRISGFRQRSTYAAVIRFIPNEIPALDSLNVPAILSSLIMEKRGLLLMVGSTGAGKSTTLAAMMDHRNETTLGH
ILTIEDPVEFLFTNKKSVVNQREVGSDTQSLQVALRNALRQAPDVILIGEIRDRETMSSAIAYAQSGHLCLATMHANNSY
QALNRILSFYPVEVRPTMLGDLAAALKAIVSQRLLRTQDGNRSPAVEVMLNTKLVSELIEKGDFSGVKEAMEKSMAEGSQ
TFEQDIARMIVEGTVTRKEGILHADSPTNLMWRLENDFAGAAKDKAKEAEEEPDDEASFTEITLDVHHGSKYGTTGFGSL

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=36.085626911315, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786296, Length=290, Percent_Identity=31.7241379310345, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789723, Length=157, Percent_Identity=40.7643312101911, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 43871; Mature: 43740

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGRTMSTMERILRLMADKKASDVYLSAHSPALIKINGQAIPINAQILPPDAPRNLLAE
CCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEEEEEEEECCCCCHHHHHH
VLPPERIEELEEVGELNMALPIAGLGNFRISGFRQRSTYAAVIRFIPNEIPALDSLNVPA
CCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILSSLIMEKRGLLLMVGSTGAGKSTTLAAMMDHRNETTLGHILTIEDPVEFLFTNKKSVV
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCHHHH
NQREVGSDTQSLQVALRNALRQAPDVILIGEIRDRETMSSAIAYAQSGHLCLATMHANNS
HHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCH
YQALNRILSFYPVEVRPTMLGDLAAALKAIVSQRLLRTQDGNRSPAVEVMLNTKLVSELI
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
EKGDFSGVKEAMEKSMAEGSQTFEQDIARMIVEGTVTRKEGILHADSPTNLMWRLENDFA
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCEECCCCCCEEEEECCCCC
GAAKDKAKEAEEEPDDEASFTEITLDVHHGSKYGTTGFGSL
CCHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TEGRTMSTMERILRLMADKKASDVYLSAHSPALIKINGQAIPINAQILPPDAPRNLLAE
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEEEEEEEECCCCCHHHHHH
VLPPERIEELEEVGELNMALPIAGLGNFRISGFRQRSTYAAVIRFIPNEIPALDSLNVPA
CCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
ILSSLIMEKRGLLLMVGSTGAGKSTTLAAMMDHRNETTLGHILTIEDPVEFLFTNKKSVV
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCHHHH
NQREVGSDTQSLQVALRNALRQAPDVILIGEIRDRETMSSAIAYAQSGHLCLATMHANNS
HHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCH
YQALNRILSFYPVEVRPTMLGDLAAALKAIVSQRLLRTQDGNRSPAVEVMLNTKLVSELI
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
EKGDFSGVKEAMEKSMAEGSQTFEQDIARMIVEGTVTRKEGILHADSPTNLMWRLENDFA
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCEECCCCCCEEEEECCCCC
GAAKDKAKEAEEEPDDEASFTEITLDVHHGSKYGTTGFGSL
CCHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]