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The map label for this gene is dapE

Identifier: 91787870

GI number: 91787870

Start: 2061320

End: 2062597

Strand: Reverse

Name: dapE

Synonym: Bpro_1994

Alternate gene names: 91787870

Gene position: 2062597-2061320 (Counterclockwise)

Preceding gene: 91787871

Following gene: 91787869

Centisome position: 39.66

GC content: 62.99

Gene sequence:

>1278_bases
ATGTCAGCTACCCTTCGCCTCACCGAACAACTGATTTCCCGGCCGTCTGTCACACCGCTCGACGAGGGCTGCATCGACCT
CCTTTCCGCCAGGCTGGGCGCGCTGGGCTTTGTGTGTGAGCGCATGGACAGCGGCCCCGACAGCTTCCGGGTTGTCAACC
TGTGGGCAAAACGCGAAGGTTTTAACCCTCTGGCCCAGGAAAACCGGGGGCAGTCAGCTACCAAATCAGCAGCAAATGAG
GGTGAAGCCCATACGACGCCCATCAAGACGCTGGTTTTCGCGGGTCACACCGACGTGGTGCCCACCGGCCCGCTGGAGCA
GTGGCACAGCCACCCGTTCACGCCCAGCCATCGCAACGGCGTGCTGTACGGCCGCGGTGCCGCCGACATGAAAACGTCGA
TTGCCGCCATGGTCGTTGCGGTCGAGGAGTTTCTGGCAGCCCACCCGCAGCCGGGGCTGTCAATTGCCTTCCTGCTCACC
AGCGACGAAGAAGGTCCGGCGACCGACGGAACCGTGGTGGTGTGCAAGCAACTGAAGGCGCGCGGCGAAGTGCTGGACTA
CTGCATCGTGGGCGAGCCTACTTCCGTCAGCCATCTCGGCGACATGATCAAGAACGGCCGCCGCGGCACCATGAGCGGCA
AGCTGACCATCAAGGGGGTGCAGGGCCATATCGCCTACCCTCACCTGGCCAGGAACCCGGTTCACCTGTTTGCACCCGCC
CTTGCCCAACTGGTGGCCACCGAGTGGGACCAGGGCAACGCTTTCTTTCCCGCCACCAGCTGGCAGGTGTCCAACATGCA
TGGCGGCACCGGCGCCTCCAACGTGATTCCGGGCGAACTGGTGGTGGATTTCAACTTCCGGTTCTGCACGGAATCCACGC
CCGAGAACCTGCAGCAGCGCCTGCAGGCAATCCTGGACCAGCACGAACTCGACTACGACCTGAAATGGACGGTGGGCGGC
CTGCCTTTTCTGACCACGCCGGGTGAGCTGGTCAACGCGGTACGCGGCGCGATTCACGCGGAAACGGGCCTGGACACCGA
GCTGTCGACAACCGGCGGCACCAGCGACGGCCGGTTCATCGCCAAGGTCTGCCCGCAGGTCATCGAATTTGGCCCGCTCA
ATGCAACCATTCACAAGATCAACGAGTGTGTGGACGTGAGCTCGCTCGACCCGCTCAAAAACATCTACAAGGGTGTCCTG
GAACGGCTGGCTGGCATAAGCGGCATGGCAGGTGCGTCAGGTCTGGCCGCAGTGGCTGATTCGACGTCCTCACCATGA

Upstream 100 bases:

>100_bases
AGGCCTCGTTCACGGAGATCACCCTCGACGTTCACCACGGCTCGAAATACGGCACCACGGGCTTCGGCTCGCTCTGAGCG
CGGGCCCGTCAACCTTTTCT

Downstream 100 bases:

>100_bases
GTTCGGCAACGCCTGTGACGGTACTCGCGCTGATCGAGCAGATGGCCGCCCGGCTGGAAGCCGCAGGGCTGAGCTTTTCG
GACGGCTTTGGGCACGGCAC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSATLRLTEQLISRPSVTPLDEGCIDLLSARLGALGFVCERMDSGPDSFRVVNLWAKREGFNPLAQENRGQSATKSAANE
GEAHTTPIKTLVFAGHTDVVPTGPLEQWHSHPFTPSHRNGVLYGRGAADMKTSIAAMVVAVEEFLAAHPQPGLSIAFLLT
SDEEGPATDGTVVVCKQLKARGEVLDYCIVGEPTSVSHLGDMIKNGRRGTMSGKLTIKGVQGHIAYPHLARNPVHLFAPA
LAQLVATEWDQGNAFFPATSWQVSNMHGGTGASNVIPGELVVDFNFRFCTESTPENLQQRLQAILDQHELDYDLKWTVGG
LPFLTTPGELVNAVRGAIHAETGLDTELSTTGGTSDGRFIAKVCPQVIEFGPLNATIHKINECVDVSSLDPLKNIYKGVL
ERLAGISGMAGASGLAAVADSTSSP

Sequences:

>Translated_425_residues
MSATLRLTEQLISRPSVTPLDEGCIDLLSARLGALGFVCERMDSGPDSFRVVNLWAKREGFNPLAQENRGQSATKSAANE
GEAHTTPIKTLVFAGHTDVVPTGPLEQWHSHPFTPSHRNGVLYGRGAADMKTSIAAMVVAVEEFLAAHPQPGLSIAFLLT
SDEEGPATDGTVVVCKQLKARGEVLDYCIVGEPTSVSHLGDMIKNGRRGTMSGKLTIKGVQGHIAYPHLARNPVHLFAPA
LAQLVATEWDQGNAFFPATSWQVSNMHGGTGASNVIPGELVVDFNFRFCTESTPENLQQRLQAILDQHELDYDLKWTVGG
LPFLTTPGELVNAVRGAIHAETGLDTELSTTGGTSDGRFIAKVCPQVIEFGPLNATIHKINECVDVSSLDPLKNIYKGVL
ERLAGISGMAGASGLAAVADSTSSP
>Mature_424_residues
SATLRLTEQLISRPSVTPLDEGCIDLLSARLGALGFVCERMDSGPDSFRVVNLWAKREGFNPLAQENRGQSATKSAANEG
EAHTTPIKTLVFAGHTDVVPTGPLEQWHSHPFTPSHRNGVLYGRGAADMKTSIAAMVVAVEEFLAAHPQPGLSIAFLLTS
DEEGPATDGTVVVCKQLKARGEVLDYCIVGEPTSVSHLGDMIKNGRRGTMSGKLTIKGVQGHIAYPHLARNPVHLFAPAL
AQLVATEWDQGNAFFPATSWQVSNMHGGTGASNVIPGELVVDFNFRFCTESTPENLQQRLQAILDQHELDYDLKWTVGGL
PFLTTPGELVNAVRGAIHAETGLDTELSTTGGTSDGRFIAKVCPQVIEFGPLNATIHKINECVDVSSLDPLKNIYKGVLE
RLAGISGMAGASGLAAVADSTSSP

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=400, Percent_Identity=52.75, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1790395, Length=375, Percent_Identity=25.8666666666667, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_POLSJ (Q12C18)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548822.1
- ProteinModelPortal:   Q12C18
- SMR:   Q12C18
- STRING:   Q12C18
- MEROPS:   M20.010
- GeneID:   4015363
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1994
- NMPDR:   fig|296591.1.peg.2611
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   Q12C18
- ProtClustDB:   PRK13009
- BioCyc:   PSP296591:BPRO_1994-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 45255; Mature: 45124

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 98-98 ACT_SITE 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLRLTEQLISRPSVTPLDEGCIDLLSARLGALGFVCERMDSGPDSFRVVNLWAKREG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
FNPLAQENRGQSATKSAANEGEAHTTPIKTLVFAGHTDVVPTGPLEQWHSHPFTPSHRNG
CCHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCC
VLYGRGAADMKTSIAAMVVAVEEFLAAHPQPGLSIAFLLTSDEEGPATDGTVVVCKQLKA
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
RGEVLDYCIVGEPTSVSHLGDMIKNGRRGTMSGKLTIKGVQGHIAYPHLARNPVHLFAPA
CCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCHHCCCCHHHHHHH
LAQLVATEWDQGNAFFPATSWQVSNMHGGTGASNVIPGELVVDFNFRFCTESTPENLQQR
HHHHHHHCCCCCCEECCCCCEEECCCCCCCCCCCCCCCEEEEEECEEECCCCCHHHHHHH
LQAILDQHELDYDLKWTVGGLPFLTTPGELVNAVRGAIHAETGLDTELSTTGGTSDGRFI
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEH
AKVCPQVIEFGPLNATIHKINECVDVSSLDPLKNIYKGVLERLAGISGMAGASGLAAVAD
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
STSSP
CCCCC
>Mature Secondary Structure 
SATLRLTEQLISRPSVTPLDEGCIDLLSARLGALGFVCERMDSGPDSFRVVNLWAKREG
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
FNPLAQENRGQSATKSAANEGEAHTTPIKTLVFAGHTDVVPTGPLEQWHSHPFTPSHRNG
CCHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCC
VLYGRGAADMKTSIAAMVVAVEEFLAAHPQPGLSIAFLLTSDEEGPATDGTVVVCKQLKA
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
RGEVLDYCIVGEPTSVSHLGDMIKNGRRGTMSGKLTIKGVQGHIAYPHLARNPVHLFAPA
CCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEECCHHCCCCHHHHHHH
LAQLVATEWDQGNAFFPATSWQVSNMHGGTGASNVIPGELVVDFNFRFCTESTPENLQQR
HHHHHHHCCCCCCEECCCCCEEECCCCCCCCCCCCCCCEEEEEECEEECCCCCHHHHHHH
LQAILDQHELDYDLKWTVGGLPFLTTPGELVNAVRGAIHAETGLDTELSTTGGTSDGRFI
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEH
AKVCPQVIEFGPLNATIHKINECVDVSSLDPLKNIYKGVLERLAGISGMAGASGLAAVAD
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
STSSP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA