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The map label for this gene is tolB

Identifier: 91787860

GI number: 91787860

Start: 2051573

End: 2052853

Strand: Direct

Name: tolB

Synonym: Bpro_1984

Alternate gene names: 91787860

Gene position: 2051573-2052853 (Clockwise)

Preceding gene: 91787859

Following gene: 91787861

Centisome position: 39.45

GC content: 61.75

Gene sequence:

>1281_bases
ATGAGCATCACACCATCACTGAGCCGTCGCACCGTCATGAGTCTGCTGGCGGCGGGCTTGTCGCCGGCATTTGCGCAGTT
CCGCGTGGAGGTGTCGGGCGTGGGCCTGACGCAATTGCCAATTGCCATCGCTTCCTTCCGGGGGGAGGTGCAGGCGCCGC
AAAAAATTGGCAACATTGTCAGGGCCGATCTTGAACGCAGTGGCATGTTTCGCCCGGTCGACACCGCGGGCATCGTGGCA
GACGAAAACACCCGGCCAGACCTCGCAATCTGGCGGCAAAAGGGCGCGGATGCGATGGTGACGGGCAGTGTCTCGCCCCT
GGCCGACGGACGATTTGATGTGCGGCTTCGCCTGTGGGATATCGTGCGGGGCCAGGACCTCGGAGGGCAGAGCTATACCG
TGGCCCAAGCTGATTTGCGCCTGTCTGCCCATCGGATTTCTGATTTTGTGTACGAGAAACTCACGGGTGAAAAAGGCATC
TTTTCAACGCGAATTGCCTATGTCACCAAAACGGCCCAACGCTTTAACCTCTGGGTGGCCGATTCGGATGGCGAAGGCGC
CCAGTCTGCCCTCACCAGCCCCGAGCCCATCATCTCGCCTTCCTGGTCGCCCAATGGCTCCCAGCTGGCTTATGTTTCCT
TCGAATCCCGCAAGCCGGTCATTTATGCGCATGATGTCGCCACCGGAAAGCGGCGCCTGCTTGCCAATTTCAGGGGCTCC
AACAGCGCACCAGCCTGGTCACCCGATGGCAAGCAACTGGTTGCCACGCTGAGCCGGGACGGCGGCTCGCAGATATACGC
CCTGGGGCTCAGTGGCGGCGACCCCAAGCGCTTGACCCAGTCATCCAGCATTGATACCGAGCCGGCTTATTCGCCCGATG
GGCGATATATCTATTTCGTCAGCGACCGCGGCGGCGCTCCGCAAATCTACCGCATGAGTCCCGGCGGTGGAAATCCGGAA
CGCGTGACGTTTACGGGCGGTTACAACATCTCCCCTGCGATCAGCCCTGATGGGCGTTGGCTGGCCTATGTTTCGAGGGT
TGGCGGCGCCTTCAAGCTGCACGTGATGGAACTTGCCAGCGGAACGGCCACTCCTATTACCGACACCACGGCCGATGAAA
GCCCGAGCTTCGCGCCCAACAGCCGCTTGATCGTGTACGCGACCCAGCAGCAGGGCCGGGAAGCACTGATGACCACGACG
CTGGACGGCAAGATCAAGGCCAGGTTATCCGGCGCCGGCGGCGACATCCGTGAGCCCGACTGGGGACCTTTCCAGCGGTG
A

Upstream 100 bases:

>100_bases
GCACATAGATCAAAGGTGGGTCTTGTCAACCGAGGGCTTCGTAAACTTGACTCCCAATCGCCCGTCAAAATAACCCAACT
CCACTGATCCAAATTCATCC

Downstream 100 bases:

>100_bases
CAGGCACTTTTTTTGAATCATCTTTTTTTGTTTTTTTGTAGAGGTACTCCATGAAGCTGAAGCGCGTTCTCTTTTCCATC
TTCTCCTCCCTGGCTCTTGC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSITPSLSRRTVMSLLAAGLSPAFAQFRVEVSGVGLTQLPIAIASFRGEVQAPQKIGNIVRADLERSGMFRPVDTAGIVA
DENTRPDLAIWRQKGADAMVTGSVSPLADGRFDVRLRLWDIVRGQDLGGQSYTVAQADLRLSAHRISDFVYEKLTGEKGI
FSTRIAYVTKTAQRFNLWVADSDGEGAQSALTSPEPIISPSWSPNGSQLAYVSFESRKPVIYAHDVATGKRRLLANFRGS
NSAPAWSPDGKQLVATLSRDGGSQIYALGLSGGDPKRLTQSSSIDTEPAYSPDGRYIYFVSDRGGAPQIYRMSPGGGNPE
RVTFTGGYNISPAISPDGRWLAYVSRVGGAFKLHVMELASGTATPITDTTADESPSFAPNSRLIVYATQQQGREALMTTT
LDGKIKARLSGAGGDIREPDWGPFQR

Sequences:

>Translated_426_residues
MSITPSLSRRTVMSLLAAGLSPAFAQFRVEVSGVGLTQLPIAIASFRGEVQAPQKIGNIVRADLERSGMFRPVDTAGIVA
DENTRPDLAIWRQKGADAMVTGSVSPLADGRFDVRLRLWDIVRGQDLGGQSYTVAQADLRLSAHRISDFVYEKLTGEKGI
FSTRIAYVTKTAQRFNLWVADSDGEGAQSALTSPEPIISPSWSPNGSQLAYVSFESRKPVIYAHDVATGKRRLLANFRGS
NSAPAWSPDGKQLVATLSRDGGSQIYALGLSGGDPKRLTQSSSIDTEPAYSPDGRYIYFVSDRGGAPQIYRMSPGGGNPE
RVTFTGGYNISPAISPDGRWLAYVSRVGGAFKLHVMELASGTATPITDTTADESPSFAPNSRLIVYATQQQGREALMTTT
LDGKIKARLSGAGGDIREPDWGPFQR
>Mature_425_residues
SITPSLSRRTVMSLLAAGLSPAFAQFRVEVSGVGLTQLPIAIASFRGEVQAPQKIGNIVRADLERSGMFRPVDTAGIVAD
ENTRPDLAIWRQKGADAMVTGSVSPLADGRFDVRLRLWDIVRGQDLGGQSYTVAQADLRLSAHRISDFVYEKLTGEKGIF
STRIAYVTKTAQRFNLWVADSDGEGAQSALTSPEPIISPSWSPNGSQLAYVSFESRKPVIYAHDVATGKRRLLANFRGSN
SAPAWSPDGKQLVATLSRDGGSQIYALGLSGGDPKRLTQSSSIDTEPAYSPDGRYIYFVSDRGGAPQIYRMSPGGGNPER
VTFTGGYNISPAISPDGRWLAYVSRVGGAFKLHVMELASGTATPITDTTADESPSFAPNSRLIVYATQQQGREALMTTTL
DGKIKARLSGAGGDIREPDWGPFQR

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=424, Percent_Identity=37.2641509433962, Blast_Score=271, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_POLSJ (Q12C28)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548812.1
- ProteinModelPortal:   Q12C28
- SMR:   Q12C28
- STRING:   Q12C28
- GeneID:   4015352
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1984
- NMPDR:   fig|296591.1.peg.2622
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q12C28
- ProtClustDB:   PRK02889
- BioCyc:   PSP296591:BPRO_1984-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- Gene3D:   G3DSA:3.40.50.10070
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 45797; Mature: 45666

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPSLSRRTVMSLLAAGLSPAFAQFRVEVSGVGLTQLPIAIASFRGEVQAPQKIGNIV
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHH
RADLERSGMFRPVDTAGIVADENTRPDLAIWRQKGADAMVTGSVSPLADGRFDVRLRLWD
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEEE
IVRGQDLGGQSYTVAQADLRLSAHRISDFVYEKLTGEKGIFSTRIAYVTKTAQRFNLWVA
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCEEEEEEEE
DSDGEGAQSALTSPEPIISPSWSPNGSQLAYVSFESRKPVIYAHDVATGKRRLLANFRGS
CCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHEECCCC
NSAPAWSPDGKQLVATLSRDGGSQIYALGLSGGDPKRLTQSSSIDTEPAYSPDGRYIYFV
CCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEE
SDRGGAPQIYRMSPGGGNPERVTFTGGYNISPAISPDGRWLAYVSRVGGAFKLHVMELAS
ECCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHHHCCCEEEEEEEEECC
GTATPITDTTADESPSFAPNSRLIVYATQQQGREALMTTTLDGKIKARLSGAGGDIREPD
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCC
WGPFQR
CCCCCC
>Mature Secondary Structure 
SITPSLSRRTVMSLLAAGLSPAFAQFRVEVSGVGLTQLPIAIASFRGEVQAPQKIGNIV
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHH
RADLERSGMFRPVDTAGIVADENTRPDLAIWRQKGADAMVTGSVSPLADGRFDVRLRLWD
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEEE
IVRGQDLGGQSYTVAQADLRLSAHRISDFVYEKLTGEKGIFSTRIAYVTKTAQRFNLWVA
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCEEEEEEEE
DSDGEGAQSALTSPEPIISPSWSPNGSQLAYVSFESRKPVIYAHDVATGKRRLLANFRGS
CCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHEECCCC
NSAPAWSPDGKQLVATLSRDGGSQIYALGLSGGDPKRLTQSSSIDTEPAYSPDGRYIYFV
CCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEE
SDRGGAPQIYRMSPGGGNPERVTFTGGYNISPAISPDGRWLAYVSRVGGAFKLHVMELAS
ECCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEHHHHHCCCEEEEEEEEECC
GTATPITDTTADESPSFAPNSRLIVYATQQQGREALMTTTLDGKIKARLSGAGGDIREPD
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCC
WGPFQR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA