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The map label for this gene is gltX

Identifier: 91787493

GI number: 91787493

Start: 1661005

End: 1662393

Strand: Direct

Name: gltX

Synonym: Bpro_1609

Alternate gene names: 91787493

Gene position: 1661005-1662393 (Clockwise)

Preceding gene: 91787492

Following gene: 91787494

Centisome position: 31.94

GC content: 62.99

Gene sequence:

>1389_bases
ATGACACAGAAAATACGAACGCGTTTTGCGCCATCGCCCACGGGCTTTATCCACCTGGGCAACATCCGCTCGGCGCTGTA
CCCGTGGGCATTTGCCCGCGCCACCGGCGGCGATTTCATTTTGCGCATTGAAGACACGGACGTGGAGCGTTCCAGCCAGG
CCGCGGTCGACGTGATCATCGAGGGCATGCGCTGGCTGGGCCTGGACCATGACGAGGGCCCTTTCTACCAGATGCAGCGC
ATGGACCGCTACAAGGCCGTGCTGGCCGAGATGCAGGCGGCCGGCCAGGTCTACCCCTGCTACATGAGCGTGGCCGAGCT
CGACGCGCTGCGCGAAAAGCAGATGGCCGCCAAGGAAAAACCGCGCTATGACGGCACCTGGCGGCCCGAGCCGGGCAAGA
CGCTGCCGCCCATTCCCGCGGGCGTGCAGCCCGTGCTGCGCTTTAAAAACCCGCAGGGCGGCTCGGTGGTCTGGGACGAC
AAGGTCAAGGGCCGCATCGAGATCAGCAACGACGAGCTCGATGACCTCGTCATTGCGCGGCCGGATGGCACACCCACCTA
CAACTTCTGCGTGGTGGTGGACGATATCGACATGGCCATCACGCATGTGATTCGCGGCGATGACCATGTGAACAACACGC
CGCGCCAGATCAATATCTTCCGCGCGCTCGGCAAGGACGTGCCGGTTTATGCCCATTTGCCCACGGTTCTGAACGAACAG
GGCGAAAAGATGAGCAAGCGCAACGGTGCCAAGCCGGTGACGCAGTACGCGGCCGAAGGTTACTTGCCCGACGCCATGGT
GAACTACCTGGCGCGCCTCGGCTGGAGTCACGGCGACGACGAGATTTTCAGCCGCGAGCAATTCCTGAAGTGGTTCAACC
TCGATCACCTCGGCAAGAGTGCCGCACAGTTCGATGAAGCGAAGCTGCGCTGGGTCAATGCACAGCACCTCAAGGCTACG
GCAGACGACAAGCTGGCCGAACTTGTGCAGCCCTTCCTGGCTGCGCGCGGCGTGGGCCTGGACACCGCACGCATGGTCGG
TGGTTGCGGGCTTTTCAAGGACCGCTGCAGCACGCTGGTGGAGCTGGCCGACTGGCTGCAATTGCTGGTGACGGGCGGAC
AGCCGAGCGCACAGGATATCAGCACCCACGTGACCGAAGCCGTGCGCCCGGCTCTGGGCAAACTGGCCGATGCGCTGGTT
GCCTGCGAGTGGAGCAAGGCGGCCATTGCGGCGGCGATCAAACAGGTGCTCGCGGAGACCGGACTGAAAATGCCGCAGCT
CGCGATGGCGGTGCGCGTGCTGGTGATGGGCACTCCGCAAACGCCCTCGCTGGATGCGATTCTGGAGCTCATGAAACGGG
AAGATGTGATCTCGCGTTTGAAGATTTGA

Upstream 100 bases:

>100_bases
GCATAGGCCAGGGCCTGATCCGCGTGGCCGCGGGCCTGGAACACATTGACGATTTGAAAGCCGATCTCCGCAGAGGTCTG
GACGGTCTGGGCCGTTTGAA

Downstream 100 bases:

>100_bases
AAAACCGCGAATTTTCGGGCTATAATTCAAGGCTCGACAACTGCTGAGAAATCTGCAGGGTTTTGGGGGTATAGCTCAGC
TGGGAGAGCGCTTGCATGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTQKIRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVIIEGMRWLGLDHDEGPFYQMQR
MDRYKAVLAEMQAAGQVYPCYMSVAELDALREKQMAAKEKPRYDGTWRPEPGKTLPPIPAGVQPVLRFKNPQGGSVVWDD
KVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKDVPVYAHLPTVLNEQ
GEKMSKRNGAKPVTQYAAEGYLPDAMVNYLARLGWSHGDDEIFSREQFLKWFNLDHLGKSAAQFDEAKLRWVNAQHLKAT
ADDKLAELVQPFLAARGVGLDTARMVGGCGLFKDRCSTLVELADWLQLLVTGGQPSAQDISTHVTEAVRPALGKLADALV
ACEWSKAAIAAAIKQVLAETGLKMPQLAMAVRVLVMGTPQTPSLDAILELMKREDVISRLKI

Sequences:

>Translated_462_residues
MTQKIRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVIIEGMRWLGLDHDEGPFYQMQR
MDRYKAVLAEMQAAGQVYPCYMSVAELDALREKQMAAKEKPRYDGTWRPEPGKTLPPIPAGVQPVLRFKNPQGGSVVWDD
KVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKDVPVYAHLPTVLNEQ
GEKMSKRNGAKPVTQYAAEGYLPDAMVNYLARLGWSHGDDEIFSREQFLKWFNLDHLGKSAAQFDEAKLRWVNAQHLKAT
ADDKLAELVQPFLAARGVGLDTARMVGGCGLFKDRCSTLVELADWLQLLVTGGQPSAQDISTHVTEAVRPALGKLADALV
ACEWSKAAIAAAIKQVLAETGLKMPQLAMAVRVLVMGTPQTPSLDAILELMKREDVISRLKI
>Mature_461_residues
TQKIRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVIIEGMRWLGLDHDEGPFYQMQRM
DRYKAVLAEMQAAGQVYPCYMSVAELDALREKQMAAKEKPRYDGTWRPEPGKTLPPIPAGVQPVLRFKNPQGGSVVWDDK
VKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKDVPVYAHLPTVLNEQG
EKMSKRNGAKPVTQYAAEGYLPDAMVNYLARLGWSHGDDEIFSREQFLKWFNLDHLGKSAAQFDEAKLRWVNAQHLKATA
DDKLAELVQPFLAARGVGLDTARMVGGCGLFKDRCSTLVELADWLQLLVTGGQPSAQDISTHVTEAVRPALGKLADALVA
CEWSKAAIAAAIKQVLAETGLKMPQLAMAVRVLVMGTPQTPSLDAILELMKREDVISRLKI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=334, Percent_Identity=35.0299401197605, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=46.8546637744035, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=27.5, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324540, Length=371, Percent_Identity=33.6927223719677, Blast_Score=209, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24665321, Length=484, Percent_Identity=28.7190082644628, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_POLSJ (Q12D45)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548445.1
- ProteinModelPortal:   Q12D45
- SMR:   Q12D45
- STRING:   Q12D45
- GeneID:   4015739
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1609
- NMPDR:   fig|296591.1.peg.202
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- PhylomeDB:   Q12D45
- ProtClustDB:   PRK01406
- BioCyc:   PSP296591:BPRO_1609-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51204; Mature: 51073

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKIRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVII
CCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
EGMRWLGLDHDEGPFYQMQRMDRYKAVLAEMQAAGQVYPCYMSVAELDALREKQMAAKEK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
PRYDGTWRPEPGKTLPPIPAGVQPVLRFKNPQGGSVVWDDKVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKDVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHH
GEKMSKRNGAKPVTQYAAEGYLPDAMVNYLARLGWSHGDDEIFSREQFLKWFNLDHLGKS
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCH
AAQFDEAKLRWVNAQHLKATADDKLAELVQPFLAARGVGLDTARMVGGCGLFKDRCSTLV
HHHHHHHHHEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHH
ELADWLQLLVTGGQPSAQDISTHVTEAVRPALGKLADALVACEWSKAAIAAAIKQVLAET
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GLKMPQLAMAVRVLVMGTPQTPSLDAILELMKREDVISRLKI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQKIRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVII
CCCHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
EGMRWLGLDHDEGPFYQMQRMDRYKAVLAEMQAAGQVYPCYMSVAELDALREKQMAAKEK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
PRYDGTWRPEPGKTLPPIPAGVQPVLRFKNPQGGSVVWDDKVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKDVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHH
GEKMSKRNGAKPVTQYAAEGYLPDAMVNYLARLGWSHGDDEIFSREQFLKWFNLDHLGKS
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCH
AAQFDEAKLRWVNAQHLKATADDKLAELVQPFLAARGVGLDTARMVGGCGLFKDRCSTLV
HHHHHHHHHEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHH
ELADWLQLLVTGGQPSAQDISTHVTEAVRPALGKLADALVACEWSKAAIAAAIKQVLAET
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GLKMPQLAMAVRVLVMGTPQTPSLDAILELMKREDVISRLKI
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA