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The map label for this gene is bioB1

Identifier: 91786799

GI number: 91786799

Start: 914985

End: 915998

Strand: Reverse

Name: bioB1

Synonym: Bpro_0897

Alternate gene names: 91786799

Gene position: 915998-914985 (Counterclockwise)

Preceding gene: 91786800

Following gene: 91786798

Centisome position: 17.61

GC content: 66.96

Gene sequence:

>1014_bases
ATGTCCTCTGTCTTGACCCAACCCCTCGCCTTCCACCCGCCCCGCCCCGCCGTCCAGCCACGGGAACACGGCCGCTGGAG
CGTTGACGAGATTGAAGCGCTGTTCAACCTGCCGTTTCCGGAGCTGATGCACCGGGCGCAAACGGTGCACCGCGAGAACT
TCGACCCCACGCGGGTTGAATTCGCCACGCTGCTGTCGGTGAAAACCGGAGGCTGCGCCGAAGACTGCGGCTACTGCCCG
CAGGCCGCCCGCTACGACACCGGCGTGGAAGCCAGCAAGCTGATGGAGCCCGCCGAAGTGCTGGCTGCCGCCCAGCGTGC
ACAGGCCGCCGGCGCCACGCGTTTCTGCATGGGCGCAGCCTGGCGTTCACCCAAGGACCGAGACATTGAAAAAGTCGCCG
AGCTGGTGCGCACCGTCAAGGCCCTGGGCCTGGAGACCTGCGCCACGCTGGGCATGCTGGAAGACGGCCACGCGCAGACA
CTGCAAAGCGCGGGCCTCGACTACTACAACCACAACCTCGACAGCGCCCCTGATTTTTACGGCGACATCATCACCACGCG
CGACTACCAGGACCGGCTGGACACGCTGGCACGCGTGCGCAGCGCCGGCGTGAAAGTCTGCTGCGGCGGCATTGTGGGCA
TGGGTGAAACCCGCCGCCAGCGCGCGGGCCTGGTGGCCGAACTGGCCAACCTCACGCCCTACCCCGAATCGGTCCCCATC
AACAACCTGGTGAAGGTTGAAGGCACGCCGCTGGCGAATCAGGCGGACCTCGATCCGTTTGAATTTGTGCGCACCATTGC
GGTGGCCCGCATCACCATGCCCACGGCGCGCGTGCGCCTGTCGGCCGGCCGGCAGCAAATGGGCGACGGCGTGCAGGCGC
TGTGTTTCCTCGCGGGGGCCAACTCGATTTTTTATGGCGACAAGCTGCTGACCACCGGCAACCCCGACACCGAGGCCGAC
GTGACCCTGCTGCAGCGCCTGGGCATGAGCCGCTCGGCGCCGGACGTTCGCTGA

Upstream 100 bases:

>100_bases
CAAGCCGTGCGAGCAAGCGCATCGAACAGACTGACAAGCTGTGTGGTACTGATAACAGTGCATCTGTGAATACTTTCAAT
ACTTTAGGAGCCCCACGTTC

Downstream 100 bases:

>100_bases
GCAGGCTTGACACCTCCCTCTAAAACGGTGGCGAAAAGGCCGGGCAAGCTGGTTAGACTCTCGCTAACTTTGGACTGAAC
CATGACTCCAGCGAACGCTC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSSVLTQPLAFHPPRPAVQPREHGRWSVDEIEALFNLPFPELMHRAQTVHRENFDPTRVEFATLLSVKTGGCAEDCGYCP
QAARYDTGVEASKLMEPAEVLAAAQRAQAAGATRFCMGAAWRSPKDRDIEKVAELVRTVKALGLETCATLGMLEDGHAQT
LQSAGLDYYNHNLDSAPDFYGDIITTRDYQDRLDTLARVRSAGVKVCCGGIVGMGETRRQRAGLVAELANLTPYPESVPI
NNLVKVEGTPLANQADLDPFEFVRTIAVARITMPTARVRLSAGRQQMGDGVQALCFLAGANSIFYGDKLLTTGNPDTEAD
VTLLQRLGMSRSAPDVR

Sequences:

>Translated_337_residues
MSSVLTQPLAFHPPRPAVQPREHGRWSVDEIEALFNLPFPELMHRAQTVHRENFDPTRVEFATLLSVKTGGCAEDCGYCP
QAARYDTGVEASKLMEPAEVLAAAQRAQAAGATRFCMGAAWRSPKDRDIEKVAELVRTVKALGLETCATLGMLEDGHAQT
LQSAGLDYYNHNLDSAPDFYGDIITTRDYQDRLDTLARVRSAGVKVCCGGIVGMGETRRQRAGLVAELANLTPYPESVPI
NNLVKVEGTPLANQADLDPFEFVRTIAVARITMPTARVRLSAGRQQMGDGVQALCFLAGANSIFYGDKLLTTGNPDTEAD
VTLLQRLGMSRSAPDVR
>Mature_336_residues
SSVLTQPLAFHPPRPAVQPREHGRWSVDEIEALFNLPFPELMHRAQTVHRENFDPTRVEFATLLSVKTGGCAEDCGYCPQ
AARYDTGVEASKLMEPAEVLAAAQRAQAAGATRFCMGAAWRSPKDRDIEKVAELVRTVKALGLETCATLGMLEDGHAQTL
QSAGLDYYNHNLDSAPDFYGDIITTRDYQDRLDTLARVRSAGVKVCCGGIVGMGETRRQRAGLVAELANLTPYPESVPIN
NLVKVEGTPLANQADLDPFEFVRTIAVARITMPTARVRLSAGRQQMGDGVQALCFLAGANSIFYGDKLLTTGNPDTEADV
TLLQRLGMSRSAPDVR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=61.1111111111111, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321725, Length=312, Percent_Identity=45.5128205128205, Blast_Score=308, Evalue=9e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB1_POLSJ (Q12F39)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547751.1
- ProteinModelPortal:   Q12F39
- SMR:   Q12F39
- STRING:   Q12F39
- GeneID:   4011205
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0897
- NMPDR:   fig|296591.1.peg.3035
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MLEDGHA
- PhylomeDB:   Q12F39
- BioCyc:   PSP296591:BPRO_0897-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36665; Mature: 36534

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVLTQPLAFHPPRPAVQPREHGRWSVDEIEALFNLPFPELMHRAQTVHRENFDPTRVE
CCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHH
FATLLSVKTGGCAEDCGYCPQAARYDTGVEASKLMEPAEVLAAAQRAQAAGATRFCMGAA
HHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
WRSPKDRDIEKVAELVRTVKALGLETCATLGMLEDGHAQTLQSAGLDYYNHNLDSAPDFY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHH
GDIITTRDYQDRLDTLARVRSAGVKVCCGGIVGMGETRRQRAGLVAELANLTPYPESVPI
HCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NNLVKVEGTPLANQADLDPFEFVRTIAVARITMPTARVRLSAGRQQMGDGVQALCFLAGA
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHCCHHHHHHHHHCC
NSIFYGDKLLTTGNPDTEADVTLLQRLGMSRSAPDVR
CCEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSVLTQPLAFHPPRPAVQPREHGRWSVDEIEALFNLPFPELMHRAQTVHRENFDPTRVE
CCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHH
FATLLSVKTGGCAEDCGYCPQAARYDTGVEASKLMEPAEVLAAAQRAQAAGATRFCMGAA
HHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
WRSPKDRDIEKVAELVRTVKALGLETCATLGMLEDGHAQTLQSAGLDYYNHNLDSAPDFY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHH
GDIITTRDYQDRLDTLARVRSAGVKVCCGGIVGMGETRRQRAGLVAELANLTPYPESVPI
HCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NNLVKVEGTPLANQADLDPFEFVRTIAVARITMPTARVRLSAGRQQMGDGVQALCFLAGA
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHCCHHHHHHHHHCC
NSIFYGDKLLTTGNPDTEADVTLLQRLGMSRSAPDVR
CCEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA