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The map label for this gene is panB

Identifier: 91786798

GI number: 91786798

Start: 914001

End: 914903

Strand: Reverse

Name: panB

Synonym: Bpro_0896

Alternate gene names: 91786798

Gene position: 914903-914001 (Counterclockwise)

Preceding gene: 91786799

Following gene: 91786797

Centisome position: 17.59

GC content: 67.44

Gene sequence:

>903_bases
ATGACTCCAGCGAACGCTCCTGCCCCGTCCCCGGCAACGCCATCGGCCACCCCTTATGGCACGCTGCCGCCCGCCGCCAC
ACCGTCGGCGCGCAAGCCGCTGAGCCTGCCGCGCCTGCGCGAGATGCATGCGCAGGGTGAAAAAATCACCATGCTGACCG
CCTACGACGCAACCTTCGCGGCCGTAGCCGACGCGGCCGGCGTGGAATGCATCCTGGTCGGCGACTCGCTCGGCATGGTC
TGCCAGGGCCTGACGAGCACCGTGGGCGTGACGCTGGAAACCATGCGCCACCACACTGAGTGCGTGGCCAACGGCCTGCG
CCGCTCGCACGCCACGGCCTGGCTGATCGGTGACCTGCCCTTTGGCACCTACCACGAGTCGAAAGAACAGGCCCTGCGCA
ACGCCGTGGCGCTGATGCAGGCCGGCGCGCACATGGTGAAGCTCGAAGGCGGCGGCTGGACGGCCGATACCGTGCGCTTT
CTGGTCGAGCGCGGCATTCCCGTCTGCGCCCACCTGGGCCTGACGCCGCAAACCGTGCATGCCCTGGGCGGTTACCGCGT
GCAGGGCAAAACCGAGGAAGCCGCCGCCACGCTCAAACGCGAGGCCCATGAACTGCAGGACGCCGGTGCGGCCATGCTGG
TGCTGGAAATGGTGCCTGCGGCCTTGTCGGCCGCGCTCACCCAGGCACTGACGCACTGCCCCACCATCGGGATTGGTGCG
GGCAATGGCACGGCCGGCCAGGTGCTGGTGCTGCATGACATGCTGGGCATGAACCTGGGCAAGATGCCCAAATTTGTACG
AAACTTCATGGACGGAGCGCCCAGTGTGCGTGGCGCCATGGAGGCTTATGTCCGCGCCGTCAAAGACGGCAGCTTCCCGG
TCAATGCCACCCACGCGTGGTAA

Upstream 100 bases:

>100_bases
GGCGCCGGACGTTCGCTGAGCAGGCTTGACACCTCCCTCTAAAACGGTGGCGAAAAGGCCGGGCAAGCTGGTTAGACTCT
CGCTAACTTTGGACTGAACC

Downstream 100 bases:

>100_bases
TCCGGACCCAGTCAAAAAAAAAGCAGCCGAGGAGACCTCATGCACATCGTTCACAGCATCGCCGAACTGCGCGAAAAACT
GGCACCCTTCCAGCGCCCGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTPANAPAPSPATPSATPYGTLPPAATPSARKPLSLPRLREMHAQGEKITMLTAYDATFAAVADAAGVECILVGDSLGMV
CQGLTSTVGVTLETMRHHTECVANGLRRSHATAWLIGDLPFGTYHESKEQALRNAVALMQAGAHMVKLEGGGWTADTVRF
LVERGIPVCAHLGLTPQTVHALGGYRVQGKTEEAAATLKREAHELQDAGAAMLVLEMVPAALSAALTQALTHCPTIGIGA
GNGTAGQVLVLHDMLGMNLGKMPKFVRNFMDGAPSVRGAMEAYVRAVKDGSFPVNATHAW

Sequences:

>Translated_300_residues
MTPANAPAPSPATPSATPYGTLPPAATPSARKPLSLPRLREMHAQGEKITMLTAYDATFAAVADAAGVECILVGDSLGMV
CQGLTSTVGVTLETMRHHTECVANGLRRSHATAWLIGDLPFGTYHESKEQALRNAVALMQAGAHMVKLEGGGWTADTVRF
LVERGIPVCAHLGLTPQTVHALGGYRVQGKTEEAAATLKREAHELQDAGAAMLVLEMVPAALSAALTQALTHCPTIGIGA
GNGTAGQVLVLHDMLGMNLGKMPKFVRNFMDGAPSVRGAMEAYVRAVKDGSFPVNATHAW
>Mature_299_residues
TPANAPAPSPATPSATPYGTLPPAATPSARKPLSLPRLREMHAQGEKITMLTAYDATFAAVADAAGVECILVGDSLGMVC
QGLTSTVGVTLETMRHHTECVANGLRRSHATAWLIGDLPFGTYHESKEQALRNAVALMQAGAHMVKLEGGGWTADTVRFL
VERGIPVCAHLGLTPQTVHALGGYRVQGKTEEAAATLKREAHELQDAGAAMLVLEMVPAALSAALTQALTHCPTIGIGAG
NGTAGQVLVLHDMLGMNLGKMPKFVRNFMDGAPSVRGAMEAYVRAVKDGSFPVNATHAW

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=45.4198473282443, Blast_Score=228, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319653, Length=274, Percent_Identity=37.5912408759124, Blast_Score=188, Evalue=1e-48,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_POLSJ (Q12F40)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547750.1
- ProteinModelPortal:   Q12F40
- SMR:   Q12F40
- STRING:   Q12F40
- GeneID:   4011204
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0896
- NMPDR:   fig|296591.1.peg.3036
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q12F40
- ProtClustDB:   PRK00311
- BioCyc:   PSP296591:BPRO_0896-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 31377; Mature: 31245

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: ACT_SITE 216-216 BINDING 118-118 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPANAPAPSPATPSATPYGTLPPAATPSARKPLSLPRLREMHAQGEKITMLTAYDATFA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHH
AVADAAGVECILVGDSLGMVCQGLTSTVGVTLETMRHHTECVANGLRRSHATAWLIGDLP
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
FGTYHESKEQALRNAVALMQAGAHMVKLEGGGWTADTVRFLVERGIPVCAHLGLTPQTVH
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHH
ALGGYRVQGKTEEAAATLKREAHELQDAGAAMLVLEMVPAALSAALTQALTHCPTIGIGA
HHCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GNGTAGQVLVLHDMLGMNLGKMPKFVRNFMDGAPSVRGAMEAYVRAVKDGSFPVNATHAW
CCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TPANAPAPSPATPSATPYGTLPPAATPSARKPLSLPRLREMHAQGEKITMLTAYDATFA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCHHHH
AVADAAGVECILVGDSLGMVCQGLTSTVGVTLETMRHHTECVANGLRRSHATAWLIGDLP
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
FGTYHESKEQALRNAVALMQAGAHMVKLEGGGWTADTVRFLVERGIPVCAHLGLTPQTVH
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHH
ALGGYRVQGKTEEAAATLKREAHELQDAGAAMLVLEMVPAALSAALTQALTHCPTIGIGA
HHCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GNGTAGQVLVLHDMLGMNLGKMPKFVRNFMDGAPSVRGAMEAYVRAVKDGSFPVNATHAW
CCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA