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The map label for this gene is hemK [H]

Identifier: 91786751

GI number: 91786751

Start: 869833

End: 870696

Strand: Reverse

Name: hemK [H]

Synonym: Bpro_0849

Alternate gene names: 91786751

Gene position: 870696-869833 (Counterclockwise)

Preceding gene: 91786752

Following gene: 91786750

Centisome position: 16.74

GC content: 67.82

Gene sequence:

>864_bases
ATGAGCCAACCGATAAACCAGCCCACCTGCGCCCAAGCCCTGAGTGCCGCCCAGGCATTGGGGCTGGATCGGCTGGACGC
CCAGTTGCTGCTGCTGCATGCGCTGGGCAAGCCCGCCATCGACCGGGCCTGGCTGCTGGCGCACGACACCGATCAGCTGA
CGGATGAAGTCGCCCAGCGTATGCGGGCATTCAGCCTGCGCCGGGCAAGGGGTGAACCCCTGGCCTATATCGTCGGCAGC
AAGGAGTTCTTTGGGCTGGAACTGCAGGTGGATGCCCGGGTGCTGGTGCCCCGCCCCGACACCGAAACGCTGGTGCATTG
GTCACTGGAAGTACTGCAGGCGCCCGCGATGACGGAACGGCCGCAGATTCTGGACCTGGGCACCGGCAGCGGCGCCATTG
CACTGGCCATCGCGCACAGTCTGCAGGCGGCCGGCCGACCAGCCCGGGTTGTTGCGGTGGACGCGAGTGCCGGCGCACTG
GCCGTGGCCCGTGAAAATGCCCGGCGCCTCCAGCTGGAACTCGAATTCAGCCAGAGCAACTGGCTGGATGAAGTTGGTGG
ACACTTTCACCTGATCGCCAGCAATCCGCCCTACATCGCCAGCGCTGACCCTCACCTGGTGGCGCTGGCGCATGAGCCTT
TGGGGTCGCTGGCCGCGGGACCAGACGGGCTGGGGGACATCCGGCAGATTGTGGCGAAGGCGCCCGAACACCTGGCGCCC
GGCGGCTGGCTGCTGCTGGAGCACGGCTATGACCAGGCGGCTGCGGTACGCGGTCTCCTGCGCGAGCGGGGCTTCGCACA
GGTCCAGAGCCGGCTGGACCTGGCCGGCATCGCACGCTGCTCGGGCGGTCAATGGCCGGGTTAA

Upstream 100 bases:

>100_bases
AGATGGCCGGCTTCCGCTTCTCACATGCCAAATCGGCCTCGGGCCCAATTTTTACCCGCGCAAGTAGCTATTGTTTTTAT
AGCAAATGCAGGACACCCCA

Downstream 100 bases:

>100_bases
GTATCCCGCGTGGAGTTCGGCAGGTCCGGACGGACCAGTAGCACCCGAGCACCGGGCCTCAAACAGCGGGATAATCAAAT
AGTTGAATTCTGATTGCCAG

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.HindHemKP [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSQPINQPTCAQALSAAQALGLDRLDAQLLLLHALGKPAIDRAWLLAHDTDQLTDEVAQRMRAFSLRRARGEPLAYIVGS
KEFFGLELQVDARVLVPRPDTETLVHWSLEVLQAPAMTERPQILDLGTGSGAIALAIAHSLQAAGRPARVVAVDASAGAL
AVARENARRLQLELEFSQSNWLDEVGGHFHLIASNPPYIASADPHLVALAHEPLGSLAAGPDGLGDIRQIVAKAPEHLAP
GGWLLLEHGYDQAAAVRGLLRERGFAQVQSRLDLAGIARCSGGQWPG

Sequences:

>Translated_287_residues
MSQPINQPTCAQALSAAQALGLDRLDAQLLLLHALGKPAIDRAWLLAHDTDQLTDEVAQRMRAFSLRRARGEPLAYIVGS
KEFFGLELQVDARVLVPRPDTETLVHWSLEVLQAPAMTERPQILDLGTGSGAIALAIAHSLQAAGRPARVVAVDASAGAL
AVARENARRLQLELEFSQSNWLDEVGGHFHLIASNPPYIASADPHLVALAHEPLGSLAAGPDGLGDIRQIVAKAPEHLAP
GGWLLLEHGYDQAAAVRGLLRERGFAQVQSRLDLAGIARCSGGQWPG
>Mature_286_residues
SQPINQPTCAQALSAAQALGLDRLDAQLLLLHALGKPAIDRAWLLAHDTDQLTDEVAQRMRAFSLRRARGEPLAYIVGSK
EFFGLELQVDARVLVPRPDTETLVHWSLEVLQAPAMTERPQILDLGTGSGAIALAIAHSLQAAGRPARVVAVDASAGALA
VARENARRLQLELEFSQSNWLDEVGGHFHLIASNPPYIASADPHLVALAHEPLGSLAAGPDGLGDIRQIVAKAPEHLAPG
GWLLLEHGYDQAAAVRGLLRERGFAQVQSRLDLAGIARCSGGQWPG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=248, Percent_Identity=33.8709677419355, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1787463, Length=280, Percent_Identity=40.3571428571429, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI87082085, Length=209, Percent_Identity=35.4066985645933, Blast_Score=91, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24582226, Length=218, Percent_Identity=31.651376146789, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30627; Mature: 30496

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPINQPTCAQALSAAQALGLDRLDAQLLLLHALGKPAIDRAWLLAHDTDQLTDEVAQR
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
MRAFSLRRARGEPLAYIVGSKEFFGLELQVDARVLVPRPDTETLVHWSLEVLQAPAMTER
HHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCC
PQILDLGTGSGAIALAIAHSLQAAGRPARVVAVDASAGALAVARENARRLQLELEFSQSN
CCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEEEEECCCC
WLDEVGGHFHLIASNPPYIASADPHLVALAHEPLGSLAAGPDGLGDIRQIVAKAPEHLAP
CHHHHCCEEEEEECCCCEEECCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHCCHHCCC
GGWLLLEHGYDQAAAVRGLLRERGFAQVQSRLDLAGIARCSGGQWPG
CCEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQPINQPTCAQALSAAQALGLDRLDAQLLLLHALGKPAIDRAWLLAHDTDQLTDEVAQR
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHH
MRAFSLRRARGEPLAYIVGSKEFFGLELQVDARVLVPRPDTETLVHWSLEVLQAPAMTER
HHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCC
PQILDLGTGSGAIALAIAHSLQAAGRPARVVAVDASAGALAVARENARRLQLELEFSQSN
CCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEEEEEECCCC
WLDEVGGHFHLIASNPPYIASADPHLVALAHEPLGSLAAGPDGLGDIRQIVAKAPEHLAP
CHHHHCCEEEEEECCCCEEECCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHCCHHCCC
GGWLLLEHGYDQAAAVRGLLRERGFAQVQSRLDLAGIARCSGGQWPG
CCEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]