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The map label for this gene is prfA [H]

Identifier: 91786752

GI number: 91786752

Start: 870795

End: 871916

Strand: Reverse

Name: prfA [H]

Synonym: Bpro_0850

Alternate gene names: 91786752

Gene position: 871916-870795 (Counterclockwise)

Preceding gene: 91786753

Following gene: 91786751

Centisome position: 16.77

GC content: 66.13

Gene sequence:

>1122_bases
ATGAAACCATTTCTTCGCCAAACCCTGGACCGCCAGCTTCTGCGGCTGCATGAACTGGATTCGCTGCTGTCAGCCACCGA
CGTCGTGAGTGACCTGGACCGCTTTCGGGCGCTGACCCGCGAGCATGCCGAAGCCAGCGTGGTGGCCGAGCAATACACCC
GGCTCACCAGCCGCGAGGCCGACCTGGCCAGCGCCGAGGGCATGCTGGCCGAAGCCAAAGACGACCCCGAGATGGCAGCC
ATGGCCGAAGAGGAAATCGCCGCCGCCAAGCTGGATATTGCCCAACTCGATGAGGCCCTGCAGCTGATGCTGATTCCCAA
GGACCCGGACGACGCGCGGGCGGCCTTTATCGAAATCCGCGCCGGCACGGGCGGCGACGAGTCGGCACTGTTTGCCGGCG
ATCTCTTTCGCATGTACACGCGCTTTGCCGAGCGCAACCGCTGGCAGGTCGAGATCATCAGCGAATCGCCCAGCGAGCTC
GGTGGCTACAAGGAAGTGGTGTTGCGCATGGACGGTCCGACCGATGCCACGGGTGTGGGCGTGTACGGCAAGCTGCGGTT
TGAATCGGGCGGCCACCGCGTGCAACGCGTGCCCGCCACCGAAACCCAGGGCCGCATCCACACCAGTGCCTGCACCGTGG
CCGTGCTGCCCGAGCCCGATGAGGCGGTGGCCATCCAGATCAACCCGGCCGACCTGCGCATAGACACCTACCGCGCCAGC
GGCGCCGGCGGCCAGCACATCAACAAGACCGACTCGGCCGTGCGCATCACGCACATCCCGACCGGCATCGTGGCCGAATG
CCAGGATGACCGCAGCCAGCACCGCAACAAGGCCAAGGCGCTGCAGGTGCTGTCAGCCCGCATCGTGGAAAAAGACCGCT
CGGAGCGCGCGGCGAAGGATGCGGCCATGCGCAAGGGCCTGGTCGGCAGCGGCGACCGCAGCGACCGCATACGCACCTAC
AACTTCCCGCAGGGGCGCCTGACCGACCACCGCATCAACCTGACGCTGTACAAGCTGCTGGCCATCATGGAAGGCGATTT
GGGCGATGTGATCACCGCGCTGCAGGTGGCGCGCGGCGCCGAACAGCTGGCAGAGCTGGAAACCGCCAGCAACGGTTTCT
AG

Upstream 100 bases:

>100_bases
GCCTTGCGCGCTGCCGCAGCCGCCAGAACGGTTGCCATCCTGCCGGCGGCTTCGCCGGTGTGCCCCCCCCATTTATCGCC
GCCCCGGCCCCCTCGATTCG

Downstream 100 bases:

>100_bases
ATGGCCGGCTTCCGCTTCTCACATGCCAAATCGGCCTCGGGCCCAATTTTTACCCGCGCAAGTAGCTATTGTTTTTATAG
CAAATGCAGGACACCCCAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKPFLRQTLDRQLLRLHELDSLLSATDVVSDLDRFRALTREHAEASVVAEQYTRLTSREADLASAEGMLAEAKDDPEMAA
MAEEEIAAAKLDIAQLDEALQLMLIPKDPDDARAAFIEIRAGTGGDESALFAGDLFRMYTRFAERNRWQVEIISESPSEL
GGYKEVVLRMDGPTDATGVGVYGKLRFESGGHRVQRVPATETQGRIHTSACTVAVLPEPDEAVAIQINPADLRIDTYRAS
GAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDRSERAAKDAAMRKGLVGSGDRSDRIRTY
NFPQGRLTDHRINLTLYKLLAIMEGDLGDVITALQVARGAEQLAELETASNGF

Sequences:

>Translated_373_residues
MKPFLRQTLDRQLLRLHELDSLLSATDVVSDLDRFRALTREHAEASVVAEQYTRLTSREADLASAEGMLAEAKDDPEMAA
MAEEEIAAAKLDIAQLDEALQLMLIPKDPDDARAAFIEIRAGTGGDESALFAGDLFRMYTRFAERNRWQVEIISESPSEL
GGYKEVVLRMDGPTDATGVGVYGKLRFESGGHRVQRVPATETQGRIHTSACTVAVLPEPDEAVAIQINPADLRIDTYRAS
GAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDRSERAAKDAAMRKGLVGSGDRSDRIRTY
NFPQGRLTDHRINLTLYKLLAIMEGDLGDVITALQVARGAEQLAELETASNGF
>Mature_373_residues
MKPFLRQTLDRQLLRLHELDSLLSATDVVSDLDRFRALTREHAEASVVAEQYTRLTSREADLASAEGMLAEAKDDPEMAA
MAEEEIAAAKLDIAQLDEALQLMLIPKDPDDARAAFIEIRAGTGGDESALFAGDLFRMYTRFAERNRWQVEIISESPSEL
GGYKEVVLRMDGPTDATGVGVYGKLRFESGGHRVQRVPATETQGRIHTSACTVAVLPEPDEAVAIQINPADLRIDTYRAS
GAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDRSERAAKDAAMRKGLVGSGDRSDRIRTY
NFPQGRLTDHRINLTLYKLLAIMEGDLGDVITALQVARGAEQLAELETASNGF

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=314, Percent_Identity=44.9044585987261, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=39.8255813953488, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=45.4106280193237, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=366, Percent_Identity=58.4699453551913, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=37.8299120234604, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=34.4827586206897, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=375, Percent_Identity=38.6666666666667, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI19921226, Length=312, Percent_Identity=40.7051282051282, Blast_Score=214, Evalue=7e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40937; Mature: 40937

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFLRQTLDRQLLRLHELDSLLSATDVVSDLDRFRALTREHAEASVVAEQYTRLTSREA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLASAEGMLAEAKDDPEMAAMAEEEIAAAKLDIAQLDEALQLMLIPKDPDDARAAFIEIR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEE
AGTGGDESALFAGDLFRMYTRFAERNRWQVEIISESPSELGGYKEVVLRMDGPTDATGVG
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEEEEECCCCCCCCCC
VYGKLRFESGGHRVQRVPATETQGRIHTSACTVAVLPEPDEAVAIQINPADLRIDTYRAS
EEEEEEECCCCCEEEECCCCCCCCEEEECEEEEEEECCCCCEEEEEECCCCEEEEEEECC
GAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDRSERAAKD
CCCCCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMRKGLVGSGDRSDRIRTYNFPQGRLTDHRINLTLYKLLAIMEGDLGDVITALQVARGA
HHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EQLAELETASNGF
HHHHHHHHCCCCC
>Mature Secondary Structure
MKPFLRQTLDRQLLRLHELDSLLSATDVVSDLDRFRALTREHAEASVVAEQYTRLTSREA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLASAEGMLAEAKDDPEMAAMAEEEIAAAKLDIAQLDEALQLMLIPKDPDDARAAFIEIR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEE
AGTGGDESALFAGDLFRMYTRFAERNRWQVEIISESPSELGGYKEVVLRMDGPTDATGVG
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEEEEECCCCCCCCCC
VYGKLRFESGGHRVQRVPATETQGRIHTSACTVAVLPEPDEAVAIQINPADLRIDTYRAS
EEEEEEECCCCCEEEECCCCCCCCEEEECEEEEEEECCCCCEEEEEECCCCEEEEEEECC
GAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDRSERAAKD
CCCCCCCCCCCCEEEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMRKGLVGSGDRSDRIRTYNFPQGRLTDHRINLTLYKLLAIMEGDLGDVITALQVARGA
HHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EQLAELETASNGF
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA