Click here to switch to the map view.

The map label for this gene is proS

Identifier: 91786735

GI number: 91786735

Start: 855674

End: 857416

Strand: Reverse

Name: proS

Synonym: Bpro_0833

Alternate gene names: 91786735

Gene position: 857416-855674 (Counterclockwise)

Preceding gene: 91786738

Following gene: 91786734

Centisome position: 16.49

GC content: 62.36

Gene sequence:

>1743_bases
ATGAAAGCCTCCCAGTTCTTCATTTCCACCCTCAAGGAAGCCCCTGCGGATGCGGAGATCGTCAGCCACCAGCTCATGAT
GCGCGCCGGTATGATCAAAAAGCTCGGTGCCGGCATATACAGCTACATGCCCATGGGTTTGCGTGTCATCCGCAAGGTCG
AGGCCATCGTTCGTGAGGAAATGAACCGCGCCGGCGCCGTGGAAATGACCATGCCGGTGATCCAGCCGGCCGAATTCTGG
CAGGAGACCGGCCGGTTTGAAAAAATGGGACCCGAACTGCTGCGCATCAAGGACCGCCATGGCCGGGATTTCGTGGTACA
GCCGACCAGCGAGGAAGTCATCACCGATGTGATGCGCCAGGACATCAAGAGCTACAAACAGCTGCCGAAGAACCTGTACC
AGATCCAGACCAAATTCCGCGACGAGCGCCGGCCGCGTTTTGGCCTGATGCGCGGGCGCGAATTCACCATGAAAGACGCC
TACAGCTTTGACCGCGACCAGGCCAGCGCCAAGGCCAGCTACCAGATCATGGCGCAGGCCTACCGGCGGATTTTTGACCG
CTTTGGCCTCACCTACCGCGCCGTGGCCGCCGACAGCGGCGCGATTGGCGGCGACCTGAGCGAAGAATTCCAGGTGATCG
CCGCCACCGGCGAAGACGCCATCGTCTACTGCCCCGGCAGCGACTATGCGGCCAATATGGAAAAAGCCGAAGCGCTGCCG
CCGCAGTGCGACCGCAAGGCTGCATCGCAGGCCATGGTCAAGACGGCCACACCGGACAAGGCGACCTGCGCCGACGTAGC
CCAGTTGCTGGGCATTCCGCTGGAAACGACCGTCAAATCCCTGGTGCTGGCCACCGACCAGACCGACGAAGCCGGCAAGA
TCATCAAGACACAGATCTGGCTGCTATTGTTGCGCGGCGACCATGACATGAATGAAGTCAAGGTCGGCAAGGTACCCGGC
CTCGCAGGGTTCCGCTTTGCCACCCTGCCAGAAATCGAGGAGCACTTTGGCTGCAAGCCCGGCTACCTGGGGCCCCTGAA
CCTTCAAAAACCGGTGAAGTTGGTGGTTGACCGTGAGGTGGCTGCACTGAGCGACTGGGTGTGCGGCGCCAACGAGATTG
ATTTCCACATCACCGGCGTGAACTGGGGACGCGATCTTGCTGAACCCGAGCTGGTGGCCGACCTGCGCAACGTGGTCGCG
GGCAACCCCTCGCCCGACGGCAAGGGCGTGCTGGCCATTGAGCGCGGCATTGAGGTGGGCCATGTGTTCTACCTCGGCAC
CAAATACAGCCAGGCCATGAATGCCACCTTCCTGGACGAAAACGGCAAGCCGCAGTTCCTGGAAATGGGCTGCTACGGCA
TCGGCATCACACGCCTGCCCGCTGCCGCCATCGAGCAAAACCACGACGAGCGCGGCATCATCTGGCCCGACGCCATTGCC
CCGTTCACGGTGGTAATTTGCCCCATCAGCCCGGACCGCTTCGCCGACGTAAAGGCCGCCGCCGACAAGCTCTATGGCGA
ACTCCTCGCCGCCGGCGTGGACGTGATCCTCGATGACCGCGGCGAGCGCCCCGGCGCGATGTTTGCCGACTGGGAGCTGA
TCGGCGTGCCGCACCGCGTCACCATTGGCGACCGTGGCCTGAAAGAAGGCCAGCTTGAATACCAGCACCGGCGTGACGCC
GCCTCCAGCAAGGTCGATGCAGCCACTGCGCTGGACTTTCTCAAGGGGAAACTGGCCCTGTGA

Upstream 100 bases:

>100_bases
TTTTTTAAACTGAGTCCATTATGCACAGCGAAGGCGCCAGCGCCAAGCGCACGGCCGGGCTCCAGCCCCCTTCCAACGCA
GAATTCCAGAAAAATAGCCC

Downstream 100 bases:

>100_bases
CAGCGCCCGGCATCGCGTCTGCAACGGCCCTCAGGCAATTTTCAAGAAGAAGGTGCCTGCAGTCCCTGTCTGCCGCGTGC
ATTCCGTTCTTATTTTCAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKASQFFISTLKEAPADAEIVSHQLMMRAGMIKKLGAGIYSYMPMGLRVIRKVEAIVREEMNRAGAVEMTMPVIQPAEFW
QETGRFEKMGPELLRIKDRHGRDFVVQPTSEEVITDVMRQDIKSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFTMKDA
YSFDRDQASAKASYQIMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPGSDYAANMEKAEALP
PQCDRKAASQAMVKTATPDKATCADVAQLLGIPLETTVKSLVLATDQTDEAGKIIKTQIWLLLLRGDHDMNEVKVGKVPG
LAGFRFATLPEIEEHFGCKPGYLGPLNLQKPVKLVVDREVAALSDWVCGANEIDFHITGVNWGRDLAEPELVADLRNVVA
GNPSPDGKGVLAIERGIEVGHVFYLGTKYSQAMNATFLDENGKPQFLEMGCYGIGITRLPAAAIEQNHDERGIIWPDAIA
PFTVVICPISPDRFADVKAAADKLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDRGLKEGQLEYQHRRDA
ASSKVDAATALDFLKGKLAL

Sequences:

>Translated_580_residues
MKASQFFISTLKEAPADAEIVSHQLMMRAGMIKKLGAGIYSYMPMGLRVIRKVEAIVREEMNRAGAVEMTMPVIQPAEFW
QETGRFEKMGPELLRIKDRHGRDFVVQPTSEEVITDVMRQDIKSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFTMKDA
YSFDRDQASAKASYQIMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPGSDYAANMEKAEALP
PQCDRKAASQAMVKTATPDKATCADVAQLLGIPLETTVKSLVLATDQTDEAGKIIKTQIWLLLLRGDHDMNEVKVGKVPG
LAGFRFATLPEIEEHFGCKPGYLGPLNLQKPVKLVVDREVAALSDWVCGANEIDFHITGVNWGRDLAEPELVADLRNVVA
GNPSPDGKGVLAIERGIEVGHVFYLGTKYSQAMNATFLDENGKPQFLEMGCYGIGITRLPAAAIEQNHDERGIIWPDAIA
PFTVVICPISPDRFADVKAAADKLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDRGLKEGQLEYQHRRDA
ASSKVDAATALDFLKGKLAL
>Mature_580_residues
MKASQFFISTLKEAPADAEIVSHQLMMRAGMIKKLGAGIYSYMPMGLRVIRKVEAIVREEMNRAGAVEMTMPVIQPAEFW
QETGRFEKMGPELLRIKDRHGRDFVVQPTSEEVITDVMRQDIKSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFTMKDA
YSFDRDQASAKASYQIMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPGSDYAANMEKAEALP
PQCDRKAASQAMVKTATPDKATCADVAQLLGIPLETTVKSLVLATDQTDEAGKIIKTQIWLLLLRGDHDMNEVKVGKVPG
LAGFRFATLPEIEEHFGCKPGYLGPLNLQKPVKLVVDREVAALSDWVCGANEIDFHITGVNWGRDLAEPELVADLRNVVA
GNPSPDGKGVLAIERGIEVGHVFYLGTKYSQAMNATFLDENGKPQFLEMGCYGIGITRLPAAAIEQNHDERGIIWPDAIA
PFTVVICPISPDRFADVKAAADKLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDRGLKEGQLEYQHRRDA
ASSKVDAATALDFLKGKLAL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=43.421052631579, Blast_Score=213, Evalue=5e-55,
Organism=Escherichia coli, GI1786392, Length=575, Percent_Identity=55.304347826087, Blast_Score=661, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=244, Percent_Identity=36.4754098360656, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=106, Percent_Identity=32.0754716981132, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=223, Percent_Identity=36.322869955157, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24656200, Length=253, Percent_Identity=36.3636363636364, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_POLSJ (Q12FA3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547687.1
- ProteinModelPortal:   Q12FA3
- SMR:   Q12FA3
- STRING:   Q12FA3
- GeneID:   4010948
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0833
- NMPDR:   fig|296591.1.peg.3101
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q12FA3
- ProtClustDB:   PRK09194
- BioCyc:   PSP296591:BPRO_0833-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64049; Mature: 64049

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFISTLKEAPADAEIVSHQLMMRAGMIKKLGAGIYSYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVEMTMPVIQPAEFWQETGRFEKMGPELLRIKDRHGRDFVVQPTSEEVITDVMRQ
HHHCCCEEEECCCCCHHHHHHHCCCHHHCCCHHEEEECCCCCEEEECCCHHHHHHHHHHH
DIKSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFTMKDAYSFDRDQASAKASYQIMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
YRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPGSDYAANMEKAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHCCC
PQCDRKAASQAMVKTATPDKATCADVAQLLGIPLETTVKSLVLATDQTDEAGKIIKTQIW
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCHHHHEEEE
LLLLRGDHDMNEVKVGKVPGLAGFRFATLPEIEEHFGCKPGYLGPLNLQKPVKLVVDREV
EEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCHHH
AALSDWVCGANEIDFHITGVNWGRDLAEPELVADLRNVVAGNPSPDGKGVLAIERGIEVG
HHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEE
HVFYLGTKYSQAMNATFLDENGKPQFLEMGCYGIGITRLPAAAIEQNHDERGIIWPDAIA
EEEEECCHHHHHCCCEEECCCCCCCEEEECCEECCHHHCCHHHHHCCCCCCCCCCCCCCC
PFTVVICPISPDRFADVKAAADKLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEECCCEEE
TIGDRGLKEGQLEYQHRRDAASSKVDAATALDFLKGKLAL
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKASQFFISTLKEAPADAEIVSHQLMMRAGMIKKLGAGIYSYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVEMTMPVIQPAEFWQETGRFEKMGPELLRIKDRHGRDFVVQPTSEEVITDVMRQ
HHHCCCEEEECCCCCHHHHHHHCCCHHHCCCHHEEEECCCCCEEEECCCHHHHHHHHHHH
DIKSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFTMKDAYSFDRDQASAKASYQIMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
YRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPGSDYAANMEKAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHCCC
PQCDRKAASQAMVKTATPDKATCADVAQLLGIPLETTVKSLVLATDQTDEAGKIIKTQIW
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCHHHHEEEE
LLLLRGDHDMNEVKVGKVPGLAGFRFATLPEIEEHFGCKPGYLGPLNLQKPVKLVVDREV
EEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCHHH
AALSDWVCGANEIDFHITGVNWGRDLAEPELVADLRNVVAGNPSPDGKGVLAIERGIEVG
HHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEE
HVFYLGTKYSQAMNATFLDENGKPQFLEMGCYGIGITRLPAAAIEQNHDERGIIWPDAIA
EEEEECCHHHHHCCCEEECCCCCCCEEEECCEECCHHHCCHHHHHCCCCCCCCCCCCCCC
PFTVVICPISPDRFADVKAAADKLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEECCCEEE
TIGDRGLKEGQLEYQHRRDAASSKVDAATALDFLKGKLAL
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA