Click here to switch to the map view.

The map label for this gene is proB

Identifier: 91786738

GI number: 91786738

Start: 858876

End: 860012

Strand: Reverse

Name: proB

Synonym: Bpro_0836

Alternate gene names: 91786738

Gene position: 860012-858876 (Counterclockwise)

Preceding gene: 91786739

Following gene: 91786735

Centisome position: 16.54

GC content: 66.14

Gene sequence:

>1137_bases
ATGCAAGAAAAGACAGGCTCTCCCGTCCTGCGCAACGCCAGACGCATTGTGGTCAAGGTGGGCTCCAGCCTGGTCACCAA
TGAAGGCCGCGGACTGGACGCGGACGCCATCGGCCTGTGGTGCGAACAGCTGGCCGCGCTGGTCAAGGATGGGCGTGAAG
TCATCATGGTGAGCAGCGGCGCCATCGCCGAAGGCATGAAGCGCCTGGGCTGGACGGCCCGCCCCAAAGCCATCCACGAG
TTGCAGGCCGCCGCCGCCGTCGGCCAGATGGGGCTGGCCCAGGTGTATGAGAGCAAGCTGCGGGAGAACGGCATCGGCAG
CGCGCAGGTCCTGCTCACGCATGCCGACCTGGCCGACCGGGAGCGTTACCTCAACGCGCGCTCCACCCTGCTGGCCTTGC
TGCAGTTGGGCGTGGTGCCGGTCATCAACGAGAACGACACCGTCGTCAACGACGAGATCAAGTTTGGCGACAACGACACC
CTGGGCGCGCTGGTCGCCAACCTTGTCGAAGCTGACGCGCTGGTCATCCTGACCGACCAGAAGGGCCTTTACACCGCCGA
CCCGCGCAAGGACCCGGCCGCCCGCTTTGTGCACGAAGCGAAGGCCGGCGATGCCGCACTGGAAGCCATGGCCGGCGGCG
CCGGCTCGAGCATTGGCAAGGGCGGCATGATCACGAAAATCCTGGCGGCCAAACGCGCGGCGGGCTCCGGCGCATCGACG
GTCATTGCCTGGGGCCGCGAGCCGCAGGCACTGATCCGGCTGACGCAGGGCGAGGCCATCGGCACGCTGCTGGTCGCGCA
AACCCAGAAGATACAGGCCCGCAAGCAATGGATGGCCGATCACCTGCAAATGCGCGGCTCGGTTGCCGTCGACGCGGGGG
CCGTCAGCAAGGTGCGCGATGAAGGCAAGAGCCTGCTGCCCATCGGCATGACCAGGGTGGACGGCGACTTTTCACGGGGC
GACGTGATTGCCATCCGGGACGCCGAAGGCGCCGAAATTGCGCGGGGCCTGGCCAACTATTCGAGTTCGGAGGCACGGCT
GATTTGCCGCAAGGCGTCTTCGGAATTCGAAAGACTTCTGGGCTACACCGGCGAGCCTGAAATGGTCCACCGGACCAACC
TCGTGCTCACGCGGTAG

Upstream 100 bases:

>100_bases
TTTTGCCAAATCCGCAGCAAGTGCGGAAGTGGCGATTGCTACATATATGATAGCTTCTTACCCTCGTATTCATTGGACTA
GAGCCTGAAAAATATCTAAA

Downstream 100 bases:

>100_bases
CTATCCGAAAGGCAGAGCACCGCCGGCATCGAAAGGTGCACCGGAGCAACCAGTCCTCCCGACCCCATACGACCGGGCGA
TGCCCCCGCACGACAGGGTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQEKTGSPVLRNARRIVVKVGSSLVTNEGRGLDADAIGLWCEQLAALVKDGREVIMVSSGAIAEGMKRLGWTARPKAIHE
LQAAAAVGQMGLAQVYESKLRENGIGSAQVLLTHADLADRERYLNARSTLLALLQLGVVPVINENDTVVNDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRKDPAARFVHEAKAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPQALIRLTQGEAIGTLLVAQTQKIQARKQWMADHLQMRGSVAVDAGAVSKVRDEGKSLLPIGMTRVDGDFSRG
DVIAIRDAEGAEIARGLANYSSSEARLICRKASSEFERLLGYTGEPEMVHRTNLVLTR

Sequences:

>Translated_378_residues
MQEKTGSPVLRNARRIVVKVGSSLVTNEGRGLDADAIGLWCEQLAALVKDGREVIMVSSGAIAEGMKRLGWTARPKAIHE
LQAAAAVGQMGLAQVYESKLRENGIGSAQVLLTHADLADRERYLNARSTLLALLQLGVVPVINENDTVVNDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRKDPAARFVHEAKAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPQALIRLTQGEAIGTLLVAQTQKIQARKQWMADHLQMRGSVAVDAGAVSKVRDEGKSLLPIGMTRVDGDFSRG
DVIAIRDAEGAEIARGLANYSSSEARLICRKASSEFERLLGYTGEPEMVHRTNLVLTR
>Mature_378_residues
MQEKTGSPVLRNARRIVVKVGSSLVTNEGRGLDADAIGLWCEQLAALVKDGREVIMVSSGAIAEGMKRLGWTARPKAIHE
LQAAAAVGQMGLAQVYESKLRENGIGSAQVLLTHADLADRERYLNARSTLLALLQLGVVPVINENDTVVNDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRKDPAARFVHEAKAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPQALIRLTQGEAIGTLLVAQTQKIQARKQWMADHLQMRGSVAVDAGAVSKVRDEGKSLLPIGMTRVDGDFSRG
DVIAIRDAEGAEIARGLANYSSSEARLICRKASSEFERLLGYTGEPEMVHRTNLVLTR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=33.9100346020761, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=44.2622950819672, Blast_Score=281, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=33.4532374100719, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=278, Percent_Identity=33.4532374100719, Blast_Score=138, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=32.6086956521739, Blast_Score=189, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=30.952380952381, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21357643, Length=296, Percent_Identity=35.472972972973, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_POLSJ (Q12FA0)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547690.1
- ProteinModelPortal:   Q12FA0
- STRING:   Q12FA0
- GeneID:   4010951
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0836
- NMPDR:   fig|296591.1.peg.3098
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q12FA0
- ProtClustDB:   PRK05429
- BioCyc:   PSP296591:BPRO_0836-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40110; Mature: 40110

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKTGSPVLRNARRIVVKVGSSLVTNEGRGLDADAIGLWCEQLAALVKDGREVIMVSSG
CCCCCCCHHHHHHHEEEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAEGMKRLGWTARPKAIHELQAAAAVGQMGLAQVYESKLRENGIGSAQVLLTHADLADR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
ERYLNARSTLLALLQLGVVPVINENDTVVNDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCCEECCEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPAARFVHEAKAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
CCCEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPQALIRLTQGEAIGTLLVAQTQKIQARKQWMADHLQMRGSVAVDAGAVSKVRD
EEEECCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHH
EGKSLLPIGMTRVDGDFSRGDVIAIRDAEGAEIARGLANYSSSEARLICRKASSEFERLL
CCCCEEEECEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GYTGEPEMVHRTNLVLTR
CCCCCCCEEEECCEEEEC
>Mature Secondary Structure
MQEKTGSPVLRNARRIVVKVGSSLVTNEGRGLDADAIGLWCEQLAALVKDGREVIMVSSG
CCCCCCCHHHHHHHEEEEECCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAEGMKRLGWTARPKAIHELQAAAAVGQMGLAQVYESKLRENGIGSAQVLLTHADLADR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
ERYLNARSTLLALLQLGVVPVINENDTVVNDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCCEECCEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPAARFVHEAKAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
CCCEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPQALIRLTQGEAIGTLLVAQTQKIQARKQWMADHLQMRGSVAVDAGAVSKVRD
EEEECCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHH
EGKSLLPIGMTRVDGDFSRGDVIAIRDAEGAEIARGLANYSSSEARLICRKASSEFERLL
CCCCEEEECEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GYTGEPEMVHRTNLVLTR
CCCCCCCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA