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The map label for this gene is metK [H]

Identifier: 91786669

GI number: 91786669

Start: 787571

End: 788752

Strand: Reverse

Name: metK [H]

Synonym: Bpro_0767

Alternate gene names: 91786669

Gene position: 788752-787571 (Counterclockwise)

Preceding gene: 91786672

Following gene: 91786668

Centisome position: 15.17

GC content: 62.77

Gene sequence:

>1182_bases
ATGGCGAACGATTTTCTCTTTACTTCCGAATCCGTCTCCGAAGGCCATCCCGACAAGGTCGCCGACCAGATTTCCGATGC
CATCCTCGATGCCATCTTCAAGCAGGATCCGCGCAGCCGCGTGGCGGCCGAGACGCTGACCAACACCGGCCTCGTCGTGC
TGGCCGGTGAAATCACCACCAATGCGCATGTCGACTACATCCAGGTCGCACGCGACACCATCAAGCGCATCGGCTACGAC
AACACCGAGTACGGCATCGACTACAAGGGCTGCGCCGTGCTCGTGGCCTACGACAAGCAGAGCAACGACATCGCCCAGGG
CGTTGACCATGCCAGCGACGATCACCTGAACATCGGCGCCGGCGACCAGGGCCTGATGTTTGGCTACGCCTGCGATGAAA
CGCCGGAACTGATGCCGGCCCCGATTTATTACGCGCACCGCCTGATGGAGCGCCAGGCCCAGCTGCGCAAGGACGGCCGC
CTGCCTTTCCTGCGCCCAGACGCCAAAAGCCAGGTCACCATGCGCTATGTGAACGGCAAACCGCACAGCATCGACACCGT
GGTGCTGTCGACCCAGCACAGCCCGGACCAGAGCGAAACGCCTCACAAGATGAAGGCCTCGTTCACCGAAGCCATCATTG
AAGAGATCATCAAGCCGGTCCTGCCCAAGGAGTGGCTCAAGGAAACCAAATACCTGATCAACCCGACCGGCCGTTTCGTC
ATTGGCGGCCCGCAGGGCGACTGCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCGCCTGCCCGCATGG
CGGCGGCGCGTTCTCGGGCAAGGATCCGAGCAAGGTCGATCGCTCGGCCGCCTATGCCGCCCGCTACGTGGCCAAAAACA
TTGTGGCCGCCGGCCTGGCCCGGCAGTGCCAGATCCAGGTGGCTTATGCGATTGGCGTGGCCCGGCCGATGAACGTGACG
GTGTACACCGAAGGCACGGGCGTGATCTCCGACGAAAAATTGTCAGCGCTGGTCCACGAACACTTTGACCTGCGTCCCAA
AGGCATCATCCAGATGCTGGACCTGCTGCGTCCAATCTACGAAAAGACCGCGGCTTACGGCCACTTCGGCCGGGATGAGC
CCGAGTTTTCCTGGGAGCGCACGGATCGGGCCGCTTTGCTGCGGGCCGCGGCAGGGCGATAA

Upstream 100 bases:

>100_bases
TACTGAACAACTTGCAGGGCGACATTAAACCAAACTGCTAAAGCGTTCGCCGAGGCACCCCAGCCGGCAACGCGCCGACA
AACTTCATGGAGTGTACGCA

Downstream 100 bases:

>100_bases
GCCCAGCCCTCCCCAAGCGGCGGACGCCGGTTCCCGCGATGGGCCACGCACTGAAAGCCACCTGCGGGTGGCTTTTTTAT
GCGTACATTTTTTGGCGCAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYD
NTEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNIGAGDQGLMFGYACDETPELMPAPIYYAHRLMERQAQLRKDGR
LPFLRPDAKSQVTMRYVNGKPHSIDTVVLSTQHSPDQSETPHKMKASFTEAIIEEIIKPVLPKEWLKETKYLINPTGRFV
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNVT
VYTEGTGVISDEKLSALVHEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGRDEPEFSWERTDRAALLRAAAGR

Sequences:

>Translated_393_residues
MANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYD
NTEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNIGAGDQGLMFGYACDETPELMPAPIYYAHRLMERQAQLRKDGR
LPFLRPDAKSQVTMRYVNGKPHSIDTVVLSTQHSPDQSETPHKMKASFTEAIIEEIIKPVLPKEWLKETKYLINPTGRFV
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNVT
VYTEGTGVISDEKLSALVHEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGRDEPEFSWERTDRAALLRAAAGR
>Mature_392_residues
ANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDN
TEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNIGAGDQGLMFGYACDETPELMPAPIYYAHRLMERQAQLRKDGRL
PFLRPDAKSQVTMRYVNGKPHSIDTVVLSTQHSPDQSETPHKMKASFTEAIIEEIIKPVLPKEWLKETKYLINPTGRFVI
GGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVARPMNVTV
YTEGTGVISDEKLSALVHEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGRDEPEFSWERTDRAALLRAAAGR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.1578947368421, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=56.578947368421, Blast_Score=407, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=67.2680412371134, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=56.1679790026247, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=397, Percent_Identity=54.1561712846348, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=55.3805774278215, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=402, Percent_Identity=54.228855721393, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=53.7313432835821, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=49.6062992125984, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=349, Percent_Identity=51.5759312320917, Blast_Score=330, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=52.5974025974026, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=60.7142857142857, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=57.2178477690289, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=56.8831168831169, Blast_Score=408, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=52.8947368421053, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=52.1052631578947, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=52.1052631578947, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=49.6062992125984, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.1428571428571, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43112; Mature: 42981

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPRSRVAAETLTNTGLVVLAGEITT
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNIGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCEEECC
GDQGLMFGYACDETPELMPAPIYYAHRLMERQAQLRKDGRLPFLRPDAKSQVTMRYVNGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCC
PHSIDTVVLSTQHSPDQSETPHKMKASFTEAIIEEIIKPVLPKEWLKETKYLINPTGRFV
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEE
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQCQIQVAYAIGVARPMNVTVYTEGTGVISDEKLSALVHEHFDLRPKGIIQMLDLLRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EKTAAYGHFGRDEPEFSWERTDRAALLRAAAGR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANDFLFTSESVSEGHPDKVADQISDAILDAIFKQDPRSRVAAETLTNTGLVVLAGEITT
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNIGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCEEECC
GDQGLMFGYACDETPELMPAPIYYAHRLMERQAQLRKDGRLPFLRPDAKSQVTMRYVNGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCC
PHSIDTVVLSTQHSPDQSETPHKMKASFTEAIIEEIIKPVLPKEWLKETKYLINPTGRFV
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEE
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQCQIQVAYAIGVARPMNVTVYTEGTGVISDEKLSALVHEHFDLRPKGIIQMLDLLRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EKTAAYGHFGRDEPEFSWERTDRAALLRAAAGR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA