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The map label for this gene is nac [H]

Identifier: 91786542

GI number: 91786542

Start: 644804

End: 645736

Strand: Direct

Name: nac [H]

Synonym: Bpro_0637

Alternate gene names: 91786542

Gene position: 644804-645736 (Clockwise)

Preceding gene: 91786541

Following gene: 91786543

Centisome position: 12.4

GC content: 68.7

Gene sequence:

>933_bases
ATGGAACTGCGCCAACTGCGTTACCTGGTCCGGGTGATCGAGCTGGGCAGCATCAGCCGCGCCGCACTGGATATGGACCT
CGTGCAATCGGCCTTGAGCCAGCAGATCAGCCGGCTGGAGAGCGAGCTCTCCACCCGTTTGCTCCAGCGCAGCCCCAAGG
GCGTGACCCCGACCGAGGCCGGCATGGCGTTTTTCCGCGAGGCGCAACTGGTGCTGCGCCACGCCGAGCAGGCGGCGCGC
GCGGCGCAGCAGTCGCGCCTGTCGGGCAGTGTCAGCGTGGGCATGGCGCCCACCACGGCAGCCGTGCTGGGGCTGCCGCT
GCTTCGCGCCATGCGTGAACGTTACCCAGATGTGCGCCTGCACATGGTGGAAAGCCTGTCGGGCCATCTCACCACCATGC
TCAATGCCCGTCAGCTGGACCTGGCCATGCTGTTCAACACCCAGGCGGGGCGCCGCTGGAGCGTGATGCCCCTGCTCGAG
GAAAAGCTGTTCCTGATCCGGTCACGCCGCACGCTGGCGGGCCAACCCGCCGACCGCATCCGTGTCGCGCAGCTGGCGGG
CCTGCCGCTGATACTGCCCACCGGCACGCACGGCTTGCGCAGCACGATCGAAAGCGCCTTTGCCCGGGCCAAATGCAAGC
CCACGCTGGTGGCCGAAATCGACTCACTGGCCATGTTGATGGATGCGGTTGACAGCGGCCTCGGCTGCACGCTGCAGCCC
TGGGCCGCGGTGAGCCGGTTCAGCGATGCGGCGCAGCGCTTTCATCTGGCGCAAATCACCGACACGCTGGCACGCCGCGT
CAATTCACTGTGCAGCCTGTCGGACGACGAACTCTCGCCCGCCGCCCTGGCTGCGCGCGTGGTGCTGGCCGATTGCGCGA
CCGAGGCCGTGCGCTCCGGCCGCTGGCTGGGTGCCCGGCTGGTGGACGCCTGA

Upstream 100 bases:

>100_bases
AGCGGAGCCGCAGCGCTCACCCTTGCCTGGCACGCCGATCACGGCGCACTCAGACGCATCCAACTGCCGTTTCTAGGTTG
AACCCACCGGAGGATTTTGC

Downstream 100 bases:

>100_bases
GGCCATCCCAGGTCTTGGCCATCACGGTTTGTGATGCCCCCATGCCGCCGACCCGCTAGGCGTGCCGGTGTTTTGGGGCT
AAATTCCAGCGTGGCTGACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MELRQLRYLVRVIELGSISRAALDMDLVQSALSQQISRLESELSTRLLQRSPKGVTPTEAGMAFFREAQLVLRHAEQAAR
AAQQSRLSGSVSVGMAPTTAAVLGLPLLRAMRERYPDVRLHMVESLSGHLTTMLNARQLDLAMLFNTQAGRRWSVMPLLE
EKLFLIRSRRTLAGQPADRIRVAQLAGLPLILPTGTHGLRSTIESAFARAKCKPTLVAEIDSLAMLMDAVDSGLGCTLQP
WAAVSRFSDAAQRFHLAQITDTLARRVNSLCSLSDDELSPAALAARVVLADCATEAVRSGRWLGARLVDA

Sequences:

>Translated_310_residues
MELRQLRYLVRVIELGSISRAALDMDLVQSALSQQISRLESELSTRLLQRSPKGVTPTEAGMAFFREAQLVLRHAEQAAR
AAQQSRLSGSVSVGMAPTTAAVLGLPLLRAMRERYPDVRLHMVESLSGHLTTMLNARQLDLAMLFNTQAGRRWSVMPLLE
EKLFLIRSRRTLAGQPADRIRVAQLAGLPLILPTGTHGLRSTIESAFARAKCKPTLVAEIDSLAMLMDAVDSGLGCTLQP
WAAVSRFSDAAQRFHLAQITDTLARRVNSLCSLSDDELSPAALAARVVLADCATEAVRSGRWLGARLVDA
>Mature_310_residues
MELRQLRYLVRVIELGSISRAALDMDLVQSALSQQISRLESELSTRLLQRSPKGVTPTEAGMAFFREAQLVLRHAEQAAR
AAQQSRLSGSVSVGMAPTTAAVLGLPLLRAMRERYPDVRLHMVESLSGHLTTMLNARQLDLAMLFNTQAGRRWSVMPLLE
EKLFLIRSRRTLAGQPADRIRVAQLAGLPLILPTGTHGLRSTIESAFARAKCKPTLVAEIDSLAMLMDAVDSGLGCTLQP
WAAVSRFSDAAQRFHLAQITDTLARRVNSLCSLSDDELSPAALAARVVLADCATEAVRSGRWLGARLVDA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=306, Percent_Identity=33.0065359477124, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=29.1497975708502, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=273, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=28.2786885245902, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=30.3149606299213, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787601, Length=210, Percent_Identity=32.3809523809524, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=28.7937743190661, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=27.6859504132231, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=242, Percent_Identity=23.9669421487603, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=178, Percent_Identity=29.7752808988764, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33833; Mature: 33833

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRYLVRVIELGSISRAALDMDLVQSALSQQISRLESELSTRLLQRSPKGVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
GMAFFREAQLVLRHAEQAARAAQQSRLSGSVSVGMAPTTAAVLGLPLLRAMRERYPDVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHH
HMVESLSGHLTTMLNARQLDLAMLFNTQAGRRWSVMPLLEEKLFLIRSRRTLAGQPADRI
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCHHHCCCCHHHH
RVAQLAGLPLILPTGTHGLRSTIESAFARAKCKPTLVAEIDSLAMLMDAVDSGLGCTLQP
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
WAAVSRFSDAAQRFHLAQITDTLARRVNSLCSLSDDELSPAALAARVVLADCATEAVRSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
RWLGARLVDA
CCCCHHCCCC
>Mature Secondary Structure
MELRQLRYLVRVIELGSISRAALDMDLVQSALSQQISRLESELSTRLLQRSPKGVTPTEA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
GMAFFREAQLVLRHAEQAARAAQQSRLSGSVSVGMAPTTAAVLGLPLLRAMRERYPDVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCHHH
HMVESLSGHLTTMLNARQLDLAMLFNTQAGRRWSVMPLLEEKLFLIRSRRTLAGQPADRI
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCHHHCCCCHHHH
RVAQLAGLPLILPTGTHGLRSTIESAFARAKCKPTLVAEIDSLAMLMDAVDSGLGCTLQP
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
WAAVSRFSDAAQRFHLAQITDTLARRVNSLCSLSDDELSPAALAARVVLADCATEAVRSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
RWLGARLVDA
CCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]