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The map label for this gene is hyuA [H]

Identifier: 91786457

GI number: 91786457

Start: 549982

End: 551418

Strand: Direct

Name: hyuA [H]

Synonym: Bpro_0551

Alternate gene names: 91786457

Gene position: 549982-551418 (Clockwise)

Preceding gene: 91786453

Following gene: 91786458

Centisome position: 10.58

GC content: 65.69

Gene sequence:

>1437_bases
ATGAGCCGCGCGCCGTTTGACCTGGTGATCCGGAATGCATGTGTTGCCACGGCCAGCGACACCTTCAAGGCCGATATCGG
GGTACTCGACGGACGGATATCGCAATTGGGCGTGGGCCTGGACCGGGGTGAGAAGGAAATCGACGCGGCTGGCCGCATCG
TGACCCCCGGAGGCGTGGACGCGCATTGCCATCTGGACCAGCCGATGGCGCCGCCGGTGCGCATGGCCGACAACTTTGAC
ACCGGCACGCGTGCCGCGGCCTGCGGCGGGACCACCACCGTGATTCCGTTCGCGGCGCAGGAAAAAGGCCAATCACTGCG
GGCCGCCGTGGCTGACTACCACCAGCGTGCCGATGGGCGCGCGCATGTCGATTACGCCTTCCACCTGATCGTCAGCGACC
CGACACCGGAAGTCCTGAACGTGGAGCTGCCGGCGCTGATCGCCGAAGGCTACACCTCGTTCAAGATCTACATGACCTAC
GACGACCTGAAGCTTGACGATGGCCAGATTCTCGACGTGCTGGACGTGGCGCGCCAGCATGGCGCCATGGCCATGGTGCA
CGCGGAGAATGCCGATTGCATCGAATGGCTGACGAAGCGGCTCGAAGCCGCCGGGCGTACCGCGCCGCGCTTTCATGCGC
ATGCGCGGCCCATGCTCGTCGAGCGGGAAGCGACACACCGGGCGATCGCGCTGTCGGAACTGGTGGATGTGCCGATTTTG
ATCGTGCATGTGTCGGGCCGTGAAGCGGTTGAGCAGATCCGCTGGGCGCGTGCCCAGGGCCTCAAGGTGTTTGCCGAAAC
CTGCCCGCAGTACCTGTTCCTGACCGCCGAGGATCTGGGGATGGACGACAGCTACCGCGGCGCGCGCTGTGTCTGCAGCC
CCCCGCCGCGCGACAAGGCCAACCAGGAGGTGATCTGGAATGGCCTCAACGATGGCCTGTTCACCCTGTTCTCGTCCGAC
CATGCGCCGTTCAACTACGAGGGCACCGAGGGCCGCAAGCCCGGTGGCGAGGAAGTGAGTTTCCGCCACATCCCCAACGG
CCTGGCCGGCATAGAGACGCGCATGGCGCTGCTCTATTCCGAAGGCGTGCTCGGCGGGCGTATCACGCTGCAAAAATTCG
TTGAACTGACTGCCACCAACCCGGCCAAGGCTTATGGCCTGCATCCCCGCAAAGGCACGATCGCCATTGGCGCCGATGCC
GACCTGGTGATCTGGGATGAACGCGAGTTCGTGCTGCGCAACACGCAGCTGCATCATGCGGTGGACCACACGCCCTATGA
AGGCATGACCCTGAAAGCCTGGCCCGGGCTCACGCTGTCGCGCGGCGAGGTGGTGTGGGACGGCCGGGACTTCCATGGCC
GGGCCGGTCGCGGCCAGTTCCTGGCCTGTGGCGCGCCCTCGCTGATGCCGTCGCGCCACGTCGGGAGGCCGGCATGA

Upstream 100 bases:

>100_bases
TGACGATGCACCAAAACAACTTAATGCAAAACATGTCGACAATTATGTTTGCACTAAAGATGACATTTATTCACCGCCTT
GGTGCGCCCGAGGTTGCGGC

Downstream 100 bases:

>100_bases
AGCTGCTGCTGATCAACCCGAATATCTCGGAGAGCGTGTCGGAGCTGATCCGCAGCGAAGCGCAACGCAGCGCTTCGGCA
GGCACCGAGATCAAGGTGCT

Product: phenylhydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRAPFDLVIRNACVATASDTFKADIGVLDGRISQLGVGLDRGEKEIDAAGRIVTPGGVDAHCHLDQPMAPPVRMADNFD
TGTRAAACGGTTTVIPFAAQEKGQSLRAAVADYHQRADGRAHVDYAFHLIVSDPTPEVLNVELPALIAEGYTSFKIYMTY
DDLKLDDGQILDVLDVARQHGAMAMVHAENADCIEWLTKRLEAAGRTAPRFHAHARPMLVEREATHRAIALSELVDVPIL
IVHVSGREAVEQIRWARAQGLKVFAETCPQYLFLTAEDLGMDDSYRGARCVCSPPPRDKANQEVIWNGLNDGLFTLFSSD
HAPFNYEGTEGRKPGGEEVSFRHIPNGLAGIETRMALLYSEGVLGGRITLQKFVELTATNPAKAYGLHPRKGTIAIGADA
DLVIWDEREFVLRNTQLHHAVDHTPYEGMTLKAWPGLTLSRGEVVWDGRDFHGRAGRGQFLACGAPSLMPSRHVGRPA

Sequences:

>Translated_478_residues
MSRAPFDLVIRNACVATASDTFKADIGVLDGRISQLGVGLDRGEKEIDAAGRIVTPGGVDAHCHLDQPMAPPVRMADNFD
TGTRAAACGGTTTVIPFAAQEKGQSLRAAVADYHQRADGRAHVDYAFHLIVSDPTPEVLNVELPALIAEGYTSFKIYMTY
DDLKLDDGQILDVLDVARQHGAMAMVHAENADCIEWLTKRLEAAGRTAPRFHAHARPMLVEREATHRAIALSELVDVPIL
IVHVSGREAVEQIRWARAQGLKVFAETCPQYLFLTAEDLGMDDSYRGARCVCSPPPRDKANQEVIWNGLNDGLFTLFSSD
HAPFNYEGTEGRKPGGEEVSFRHIPNGLAGIETRMALLYSEGVLGGRITLQKFVELTATNPAKAYGLHPRKGTIAIGADA
DLVIWDEREFVLRNTQLHHAVDHTPYEGMTLKAWPGLTLSRGEVVWDGRDFHGRAGRGQFLACGAPSLMPSRHVGRPA
>Mature_477_residues
SRAPFDLVIRNACVATASDTFKADIGVLDGRISQLGVGLDRGEKEIDAAGRIVTPGGVDAHCHLDQPMAPPVRMADNFDT
GTRAAACGGTTTVIPFAAQEKGQSLRAAVADYHQRADGRAHVDYAFHLIVSDPTPEVLNVELPALIAEGYTSFKIYMTYD
DLKLDDGQILDVLDVARQHGAMAMVHAENADCIEWLTKRLEAAGRTAPRFHAHARPMLVEREATHRAIALSELVDVPILI
VHVSGREAVEQIRWARAQGLKVFAETCPQYLFLTAEDLGMDDSYRGARCVCSPPPRDKANQEVIWNGLNDGLFTLFSSDH
APFNYEGTEGRKPGGEEVSFRHIPNGLAGIETRMALLYSEGVLGGRITLQKFVELTATNPAKAYGLHPRKGTIAIGADAD
LVIWDEREFVLRNTQLHHAVDHTPYEGMTLKAWPGLTLSRGEVVWDGRDFHGRAGRGQFLACGAPSLMPSRHVGRPA

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=468, Percent_Identity=36.5384615384615, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4503377, Length=460, Percent_Identity=34.3478260869565, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI4503051, Length=460, Percent_Identity=33.4782608695652, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI62422571, Length=460, Percent_Identity=33.4782608695652, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=32.9004329004329, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI190194363, Length=463, Percent_Identity=34.1252699784017, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI19923821, Length=467, Percent_Identity=35.5460385438972, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI18105007, Length=397, Percent_Identity=25.6926952141058, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87082175, Length=459, Percent_Identity=39.6514161220044, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1786722, Length=468, Percent_Identity=31.6239316239316, Blast_Score=193, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17539558, Length=463, Percent_Identity=33.0453563714903, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71989490, Length=471, Percent_Identity=33.5456475583864, Blast_Score=231, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI86575075, Length=458, Percent_Identity=27.292576419214, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI193204318, Length=399, Percent_Identity=23.8095238095238, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322218, Length=475, Percent_Identity=27.3684210526316, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI221377917, Length=468, Percent_Identity=35.6837606837607, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI17137462, Length=467, Percent_Identity=34.6895074946467, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24644287, Length=314, Percent_Identity=35.6687898089172, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24644289, Length=234, Percent_Identity=37.1794871794872, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24642586, Length=416, Percent_Identity=26.2019230769231, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI18859883, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- InterPro:   IPR011612 [H]

Pfam domain/function: PF01979 Amidohydro_1; PF00449 Urease_alpha [H]

EC number: 3.5.2.2

Molecular weight: Translated: 52154; Mature: 52022

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPFDLVIRNACVATASDTFKADIGVLDGRISQLGVGLDRGEKEIDAAGRIVTPGGVD
CCCCCHHHHHHHHHEECCCCCCEECHHHHCCCHHHHCCCCCCCCHHHHCCCCEECCCCCC
AHCHLDQPMAPPVRMADNFDTGTRAAACGGTTTVIPFAAQEKGQSLRAAVADYHQRADGR
EEEECCCCCCCCCEECCCCCCCCCEEECCCCEEEEEECCHHCCHHHHHHHHHHHHHCCCC
AHVDYAFHLIVSDPTPEVLNVELPALIAEGYTSFKIYMTYDDLKLDDGQILDVLDVARQH
EEEEEEEEEEECCCCCCEEEEEHHHHHHCCCCEEEEEEEECCEECCCCCHHHHHHHHHHC
GAMAMVHAENADCIEWLTKRLEAAGRTAPRFHAHARPMLVEREATHRAIALSELVDVPIL
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCEEEECCHHHHHHHHHHHCCCCEE
IVHVSGREAVEQIRWARAQGLKVFAETCPQYLFLTAEDLGMDDSYRGARCVCSPPPRDKA
EEEECCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEECCCCCCCCC
NQEVIWNGLNDGLFTLFSSDHAPFNYEGTEGRKPGGEEVSFRHIPNGLAGIETRMALLYS
CCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEC
EGVLGGRITLQKFVELTATNPAKAYGLHPRKGTIAIGADADLVIWDEREFVLRNTQLHHA
CCCCCCEEHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCCEEEECCCEEEEECCHHHHH
VDHTPYEGMTLKAWPGLTLSRGEVVWDGRDFHGRAGRGQFLACGAPSLMPSRHVGRPA
HCCCCCCCEEEEECCCCEEECCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRAPFDLVIRNACVATASDTFKADIGVLDGRISQLGVGLDRGEKEIDAAGRIVTPGGVD
CCCCHHHHHHHHHEECCCCCCEECHHHHCCCHHHHCCCCCCCCHHHHCCCCEECCCCCC
AHCHLDQPMAPPVRMADNFDTGTRAAACGGTTTVIPFAAQEKGQSLRAAVADYHQRADGR
EEEECCCCCCCCCEECCCCCCCCCEEECCCCEEEEEECCHHCCHHHHHHHHHHHHHCCCC
AHVDYAFHLIVSDPTPEVLNVELPALIAEGYTSFKIYMTYDDLKLDDGQILDVLDVARQH
EEEEEEEEEEECCCCCCEEEEEHHHHHHCCCCEEEEEEEECCEECCCCCHHHHHHHHHHC
GAMAMVHAENADCIEWLTKRLEAAGRTAPRFHAHARPMLVEREATHRAIALSELVDVPIL
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCEEEECCHHHHHHHHHHHCCCCEE
IVHVSGREAVEQIRWARAQGLKVFAETCPQYLFLTAEDLGMDDSYRGARCVCSPPPRDKA
EEEECCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEECCCCCCCCC
NQEVIWNGLNDGLFTLFSSDHAPFNYEGTEGRKPGGEEVSFRHIPNGLAGIETRMALLYS
CCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEC
EGVLGGRITLQKFVELTATNPAKAYGLHPRKGTIAIGADADLVIWDEREFVLRNTQLHHA
CCCCCCEEHHHHHHHHHCCCCHHHCCCCCCCCEEEECCCCCEEEECCCEEEEECCHHHHH
VDHTPYEGMTLKAWPGLTLSRGEVVWDGRDFHGRAGRGQFLACGAPSLMPSRHVGRPA
HCCCCCCCEEEEECCCCEEECCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA