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The map label for this gene is dhaAF [H]

Identifier: 91786453

GI number: 91786453

Start: 545640

End: 546710

Strand: Direct

Name: dhaAF [H]

Synonym: Bpro_0547

Alternate gene names: 91786453

Gene position: 545640-546710 (Clockwise)

Preceding gene: 91786452

Following gene: 91786457

Centisome position: 10.49

GC content: 58.92

Gene sequence:

>1071_bases
ATGTCCTCGCCCACGTCATCTCAGCAAACTAACTCGACGGACCAGGGAGCCAATTCCCACCGCCGCCAATTTCTCAACCT
GACTGCCATTGGTTTGACTGGAGCCGCAGCGCTTGCAGCATACCCATTGGTATCCGCAGCTTCTTCTACAACGTCGGCCA
TTAACTCGAAAGGAACCCCCATGTCGACCTCAAACCTCCAGAACGCCAGCGCAAACGCACCACGCGCAAGCTTCCACTCC
GTGGAAGCTGATGGTGTCAAGGTCTTCTATCGCGAGAGCGGTCCGGCCCAAGCGCCGGTGATTCTTCTCCTACACGGCTT
TCCAGCGTCGTCACACATGTTCCGCGAGTTGATCCCGCGTTTGGCATCCAAATACCGTGTTATTGCGCCGGACCTCCCGG
GATTTGGTTTCACGTCGGTACCTGATACCAGGCACTACGCTTACTCGTTCGATTCGTTGGCACGGACTACAGAGGCATTC
GTAGAGGCTTTGGGACTCAAACGCTACGCCCTGTATGTTTTCGACTACGGCGCGCCAGTGGGCTTCCGACTGGCACTTGC
TCACCCAGATCGGGTGACCGCCATCGTTTCCCAGAATGGCAATGCCTATGAGGAGGGTCTTGGTGAGGCGTGGGCACCGT
TCCAACAATACTGGGCGGCCCCCAACTCCGAAAACCGAAAGGCGCTGCTCAGTGCCTTGACATTGGAGACGACGCACTGG
GCTTATACACACGGGCTCCCCAATCCGCAAGCAGTGGCGCCGGAGTCTTACCAACTCGACGCGGCCCTGATGGCTCGCCC
GGGCAACTCCGAGGTTCAGCTCGACCTGTTCCTGGACTACGCCTCCAACCTGAAGCGCTACCCGGCGTTTCAGGCCTACT
TCCGCGAGGTGAAACCGCGCCTGCTGGCGATCTGGGGCAAGTACGACCAGTTGTTCATCCCTGCCGGCGCCCATGCTTTC
AAGCGCGACAACCCCAACGCCGTTGTCAAGCTCCTCGACGCCAGCCATTTCGCGCTGGAGACGCACGTTGAAGAGATTGC
GGCCGAGATGCACGTTTTTCTGCGGAGCTAG

Upstream 100 bases:

>100_bases
TTTCCAATGGCCCTTTTCTGTTCTCTGCTCACCCGTTTCGGCGGACGTGCAGGTGCCTTGCCCCGCTGTGTGCCGCAACA
GCTGACGTGAGGAGCAATAG

Downstream 100 bases:

>100_bases
CCCAGTGTCGCTGTGGGCTGCGCCGAGGCTGCCAGAAACTCCATGGCCGCCGAGGTGCCGCTCTCGCGCAGACGCACGCC
TGCGAGTTTCAGGCCCATCT

Product: twin-arginine translocation pathway signal protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSPTSSQQTNSTDQGANSHRRQFLNLTAIGLTGAAALAAYPLVSAASSTTSAINSKGTPMSTSNLQNASANAPRASFHS
VEADGVKVFYRESGPAQAPVILLLHGFPASSHMFRELIPRLASKYRVIAPDLPGFGFTSVPDTRHYAYSFDSLARTTEAF
VEALGLKRYALYVFDYGAPVGFRLALAHPDRVTAIVSQNGNAYEEGLGEAWAPFQQYWAAPNSENRKALLSALTLETTHW
AYTHGLPNPQAVAPESYQLDAALMARPGNSEVQLDLFLDYASNLKRYPAFQAYFREVKPRLLAIWGKYDQLFIPAGAHAF
KRDNPNAVVKLLDASHFALETHVEEIAAEMHVFLRS

Sequences:

>Translated_356_residues
MSSPTSSQQTNSTDQGANSHRRQFLNLTAIGLTGAAALAAYPLVSAASSTTSAINSKGTPMSTSNLQNASANAPRASFHS
VEADGVKVFYRESGPAQAPVILLLHGFPASSHMFRELIPRLASKYRVIAPDLPGFGFTSVPDTRHYAYSFDSLARTTEAF
VEALGLKRYALYVFDYGAPVGFRLALAHPDRVTAIVSQNGNAYEEGLGEAWAPFQQYWAAPNSENRKALLSALTLETTHW
AYTHGLPNPQAVAPESYQLDAALMARPGNSEVQLDLFLDYASNLKRYPAFQAYFREVKPRLLAIWGKYDQLFIPAGAHAF
KRDNPNAVVKLLDASHFALETHVEEIAAEMHVFLRS
>Mature_355_residues
SSPTSSQQTNSTDQGANSHRRQFLNLTAIGLTGAAALAAYPLVSAASSTTSAINSKGTPMSTSNLQNASANAPRASFHSV
EADGVKVFYRESGPAQAPVILLLHGFPASSHMFRELIPRLASKYRVIAPDLPGFGFTSVPDTRHYAYSFDSLARTTEAFV
EALGLKRYALYVFDYGAPVGFRLALAHPDRVTAIVSQNGNAYEEGLGEAWAPFQQYWAAPNSENRKALLSALTLETTHWA
YTHGLPNPQAVAPESYQLDAALMARPGNSEVQLDLFLDYASNLKRYPAFQAYFREVKPRLLAIWGKYDQLFIPAGAHAFK
RDNPNAVVKLLDASHFALETHVEEIAAEMHVFLRS

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=119, Percent_Identity=30.2521008403361, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI27597073, Length=127, Percent_Identity=35.4330708661417, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562298, Length=290, Percent_Identity=25.1724137931034, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17554012, Length=292, Percent_Identity=23.972602739726, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324392, Length=280, Percent_Identity=49.6428571428571, Blast_Score=278, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 38897; Mature: 38766

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTSSQQTNSTDQGANSHRRQFLNLTAIGLTGAAALAAYPLVSAASSTTSAINSKGTP
CCCCCCCCCCCCCCCCCHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
MSTSNLQNASANAPRASFHSVEADGVKVFYRESGPAQAPVILLLHGFPASSHMFRELIPR
CCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHH
LASKYRVIAPDLPGFGFTSVPDTRHYAYSFDSLARTTEAFVEALGLKRYALYVFDYGAPV
HHHCCEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
GFRLALAHPDRVTAIVSQNGNAYEEGLGEAWAPFQQYWAAPNSENRKALLSALTLETTHW
CEEEEEECCCEEEEEEECCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
AYTHGLPNPQAVAPESYQLDAALMARPGNSEVQLDLFLDYASNLKRYPAFQAYFREVKPR
EEECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEHHHHHHHCHHHHHHHHHHCCC
LLAIWGKYDQLFIPAGAHAFKRDNPNAVVKLLDASHFALETHVEEIAAEMHVFLRS
EEEEECCCCEEEECCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPTSSQQTNSTDQGANSHRRQFLNLTAIGLTGAAALAAYPLVSAASSTTSAINSKGTP
CCCCCCCCCCCCCCCCHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
MSTSNLQNASANAPRASFHSVEADGVKVFYRESGPAQAPVILLLHGFPASSHMFRELIPR
CCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHH
LASKYRVIAPDLPGFGFTSVPDTRHYAYSFDSLARTTEAFVEALGLKRYALYVFDYGAPV
HHHCCEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
GFRLALAHPDRVTAIVSQNGNAYEEGLGEAWAPFQQYWAAPNSENRKALLSALTLETTHW
CEEEEEECCCEEEEEEECCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
AYTHGLPNPQAVAPESYQLDAALMARPGNSEVQLDLFLDYASNLKRYPAFQAYFREVKPR
EEECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEHHHHHHHCHHHHHHHHHHCCC
LLAIWGKYDQLFIPAGAHAFKRDNPNAVVKLLDASHFALETHVEEIAAEMHVFLRS
EEEEECCCCEEEECCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]