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The map label for this gene is glpK

Identifier: 91786386

GI number: 91786386

Start: 491944

End: 493437

Strand: Reverse

Name: glpK

Synonym: Bpro_0476

Alternate gene names: 91786386

Gene position: 493437-491944 (Counterclockwise)

Preceding gene: 91786387

Following gene: 91786385

Centisome position: 9.49

GC content: 66.6

Gene sequence:

>1494_bases
ATGACTTATTTGCTCGCCCTTGACCAGGGCACCTCCAGCTCCCGCAGCATTGTGTTTGACGAGCTGGGCCACATCGTGGC
CCAGGCCCAGCAGGAGCTGCCCCAGATCTATCCCAAACCAGGCTGGGTCGAACATGACCCGATGGAAATCTGGCGCACCC
AACTGGCCACGGCGCGCGAAGCGCTCGGCAAAGCGGGACTGAAAGCGAGCGACATTCGCGCCCTGGGCATCACCAACCAG
CGCGAAACCACGGTGGTGTGGCACCGCGCGACCGGCCAACCCATCCATAACGCCATCGTCTGGCAGGACCGTCGCGCCGA
AGCGACCTGCGCGCAACTGCGCGAGCAGGGCAAGGAAGCGCTGATCCAGTCCAAGACCGGCTTGTTGATTGATGCCTATT
TTTCGGGCACCAAGCTCAAGTGGCTGCTCGACAACGTACCCGGCGTGCGCGCGCAGGCCGAACGCGGCGAGCTGGCCTTT
GGCACCATCGACAGCTGGCTGATGTGGCAACTGACGGGCGGCGCGCTGCACGCCACTGACGTCAGCAATGCCTCGCGCAC
CATGCTGTTCAATGTGCGCACCAACCAGTGGGACGCCGAGCTGCTGGACCTGCTGGGCATTCCCGCTTCGCTCATGCCCC
AGGTGCTGCCGTCCAGCGCGCACTACGGCGAAACCCGGCCCGAGCTGCTGGGCCACGCGATAGCGATCGGCGGCGTCGCC
GGCGACCAGCAAAGTGCGCTGTTTGGCCAGGCCTGCTTCAAGGCAGGCATGGCCAAAAACACCTATGGCACCGGCTGCTT
CATGCTGATGCACACCGGCACGCAATTCCAGACCTCGCACAACGGCCTGCTGACCACCAGCGCCGCGCAAACCACGGCGC
ACCCCGAATTTGCCATGGAGGGCAGCGTGTTTGTCGGCGGTGCGGTGGTGCAGTGGCTGCGCGATGGCCTGCACGCCATC
CAGGGCAGCGGCGAGGTGCAGGCGCTGGCGCAAAGCGTGCCCGACTCCGGCGGCGTCATGATGGTGCCGGCCTTCACCGG
CCTGGGCGCCCCCTACTGGAAACCCGATGCCCGCGGCACCATCACCGGGCTGACCCGCGGCACCACTGTGGCCCATATTG
CGCGTGCCGCGCTGGAAAGCATTGCCTACCAGAGTGCCGCCCTGCTGCAGGCCATGAGCCGGGATGCGGTGAGCAGCGGT
GCGGCGCCCCTGGCCGAGCTGCGCGTTGACGGTGGGGCGTGCGCCAATGATTTGCTGATGCAGTTCCAGGCCGACCTGCT
GGGCATTCCGGTGCTGCGACCGGCGGTGATTGAAACCACGGCCCTGGGCGCGGCTTACCTGGCCGGCCTTTCCAGCGGGG
TGTACCGCAGCACGGACGAGCTGTCCGGCATGTGGCGCGCGGAGCGGACGTTTTTGCCTACCCTGGGCGCCGACAGCGCG
GCGGCGTTGATGAATCGGTGGGAGCATGCCGTCAGGCAGACGGTGCTTCCGTAA

Upstream 100 bases:

>100_bases
CCTGCTGGAAGAGGCCAATGTGATCTGTGAAATAGCCCACGCCTGAGGCAGCATCAGATCCGTCAGCCCCATCAAGCCCC
TACCCCCGCCGATTGAAGCC

Downstream 100 bases:

>100_bases
GCCCGGGCCTGCCGGGTCGGCGACAATTCGTTTTCGGGCGGAAACTTCAACGCCGCCGAACTAAAGCGAGCGACACGAAC
ATGAGCACCCCAAACCCAGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTYLLALDQGTSSSRSIVFDELGHIVAQAQQELPQIYPKPGWVEHDPMEIWRTQLATAREALGKAGLKASDIRALGITNQ
RETTVVWHRATGQPIHNAIVWQDRRAEATCAQLREQGKEALIQSKTGLLIDAYFSGTKLKWLLDNVPGVRAQAERGELAF
GTIDSWLMWQLTGGALHATDVSNASRTMLFNVRTNQWDAELLDLLGIPASLMPQVLPSSAHYGETRPELLGHAIAIGGVA
GDQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTQFQTSHNGLLTTSAAQTTAHPEFAMEGSVFVGGAVVQWLRDGLHAI
QGSGEVQALAQSVPDSGGVMMVPAFTGLGAPYWKPDARGTITGLTRGTTVAHIARAALESIAYQSAALLQAMSRDAVSSG
AAPLAELRVDGGACANDLLMQFQADLLGIPVLRPAVIETTALGAAYLAGLSSGVYRSTDELSGMWRAERTFLPTLGADSA
AALMNRWEHAVRQTVLP

Sequences:

>Translated_497_residues
MTYLLALDQGTSSSRSIVFDELGHIVAQAQQELPQIYPKPGWVEHDPMEIWRTQLATAREALGKAGLKASDIRALGITNQ
RETTVVWHRATGQPIHNAIVWQDRRAEATCAQLREQGKEALIQSKTGLLIDAYFSGTKLKWLLDNVPGVRAQAERGELAF
GTIDSWLMWQLTGGALHATDVSNASRTMLFNVRTNQWDAELLDLLGIPASLMPQVLPSSAHYGETRPELLGHAIAIGGVA
GDQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTQFQTSHNGLLTTSAAQTTAHPEFAMEGSVFVGGAVVQWLRDGLHAI
QGSGEVQALAQSVPDSGGVMMVPAFTGLGAPYWKPDARGTITGLTRGTTVAHIARAALESIAYQSAALLQAMSRDAVSSG
AAPLAELRVDGGACANDLLMQFQADLLGIPVLRPAVIETTALGAAYLAGLSSGVYRSTDELSGMWRAERTFLPTLGADSA
AALMNRWEHAVRQTVLP
>Mature_496_residues
TYLLALDQGTSSSRSIVFDELGHIVAQAQQELPQIYPKPGWVEHDPMEIWRTQLATAREALGKAGLKASDIRALGITNQR
ETTVVWHRATGQPIHNAIVWQDRRAEATCAQLREQGKEALIQSKTGLLIDAYFSGTKLKWLLDNVPGVRAQAERGELAFG
TIDSWLMWQLTGGALHATDVSNASRTMLFNVRTNQWDAELLDLLGIPASLMPQVLPSSAHYGETRPELLGHAIAIGGVAG
DQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTQFQTSHNGLLTTSAAQTTAHPEFAMEGSVFVGGAVVQWLRDGLHAIQ
GSGEVQALAQSVPDSGGVMMVPAFTGLGAPYWKPDARGTITGLTRGTTVAHIARAALESIAYQSAALLQAMSRDAVSSGA
APLAELRVDGGACANDLLMQFQADLLGIPVLRPAVIETTALGAAYLAGLSSGVYRSTDELSGMWRAERTFLPTLGADSAA
ALMNRWEHAVRQTVLP

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=45.3280318091451, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=45.3280318091451, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI42794763, Length=511, Percent_Identity=44.6183953033268, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=45.7256461232604, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=33.0058939096267, Blast_Score=305, Evalue=7e-83,
Organism=Escherichia coli, GI1790361, Length=492, Percent_Identity=54.6747967479675, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1790005, Length=518, Percent_Identity=26.0617760617761, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787789, Length=517, Percent_Identity=27.852998065764, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789987, Length=504, Percent_Identity=27.3809523809524, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789168, Length=473, Percent_Identity=23.8900634249471, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=40.7920792079208, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71986140, Length=498, Percent_Identity=24.8995983935743, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=302, Percent_Identity=27.8145695364238, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321755, Length=482, Percent_Identity=41.0788381742739, Blast_Score=336, Evalue=5e-93,
Organism=Drosophila melanogaster, GI17864214, Length=463, Percent_Identity=47.3002159827214, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=401, Percent_Identity=48.6284289276808, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=482, Percent_Identity=41.0788381742739, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655560, Length=482, Percent_Identity=41.0788381742739, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655570, Length=482, Percent_Identity=41.0788381742739, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655574, Length=482, Percent_Identity=41.0788381742739, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655565, Length=482, Percent_Identity=41.0788381742739, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI19922058, Length=508, Percent_Identity=33.4645669291339, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24652871, Length=490, Percent_Identity=33.469387755102, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=29.9610894941634, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=28.6527514231499, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24656372, Length=347, Percent_Identity=30.2593659942363, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45552909, Length=294, Percent_Identity=27.891156462585, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_POLSJ (Q12GA2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547338.1
- ProteinModelPortal:   Q12GA2
- SMR:   Q12GA2
- STRING:   Q12GA2
- GeneID:   4011499
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0476
- NMPDR:   fig|296591.1.peg.4475
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   QDRRTEE
- PhylomeDB:   Q12GA2
- ProtClustDB:   CLSK897540
- BioCyc:   PSP296591:BPRO_0476-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53113; Mature: 52982

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLLALDQGTSSSRSIVFDELGHIVAQAQQELPQIYPKPGWVEHDPMEIWRTQLATARE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALGKAGLKASDIRALGITNQRETTVVWHRATGQPIHNAIVWQDRRAEATCAQLREQGKEA
HHHHCCCCHHCEEEECCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHHHHHH
LIQSKTGLLIDAYFSGTKLKWLLDNVPGVRAQAERGELAFGTIDSWLMWQLTGGALHATD
HHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCEEEECC
VSNASRTMLFNVRTNQWDAELLDLLGIPASLMPQVLPSSAHYGETRPELLGHAIAIGGVA
CCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCC
GDQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTQFQTSHNGLLTTSAAQTTAHPEFAME
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCEEC
GSVFVGGAVVQWLRDGLHAIQGSGEVQALAQSVPDSGGVMMVPAFTGLGAPYWKPDARGT
CCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCE
ITGLTRGTTVAHIARAALESIAYQSAALLQAMSRDAVSSGAAPLAELRVDGGACANDLLM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHH
QFQADLLGIPVLRPAVIETTALGAAYLAGLSSGVYRSTDELSGMWRAERTFLPTLGADSA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCHH
AALMNRWEHAVRQTVLP
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYLLALDQGTSSSRSIVFDELGHIVAQAQQELPQIYPKPGWVEHDPMEIWRTQLATARE
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALGKAGLKASDIRALGITNQRETTVVWHRATGQPIHNAIVWQDRRAEATCAQLREQGKEA
HHHHCCCCHHCEEEECCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHHHHHH
LIQSKTGLLIDAYFSGTKLKWLLDNVPGVRAQAERGELAFGTIDSWLMWQLTGGALHATD
HHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCEEEECC
VSNASRTMLFNVRTNQWDAELLDLLGIPASLMPQVLPSSAHYGETRPELLGHAIAIGGVA
CCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCC
GDQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTQFQTSHNGLLTTSAAQTTAHPEFAME
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCEEC
GSVFVGGAVVQWLRDGLHAIQGSGEVQALAQSVPDSGGVMMVPAFTGLGAPYWKPDARGT
CCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCE
ITGLTRGTTVAHIARAALESIAYQSAALLQAMSRDAVSSGAAPLAELRVDGGACANDLLM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHH
QFQADLLGIPVLRPAVIETTALGAAYLAGLSSGVYRSTDELSGMWRAERTFLPTLGADSA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCHH
AALMNRWEHAVRQTVLP
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA