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The map label for this gene is bkdA2 [H]

Identifier: 91786185

GI number: 91786185

Start: 290254

End: 291264

Strand: Direct

Name: bkdA2 [H]

Synonym: Bpro_0274

Alternate gene names: 91786185

Gene position: 290254-291264 (Clockwise)

Preceding gene: 91786184

Following gene: 91786186

Centisome position: 5.58

GC content: 66.47

Gene sequence:

>1011_bases
ATGCAGATGACCATGATCCAGGCACTGCGCTCGGCGATGGACGTGATGCTGGAACGTGACAGCAATGTCGTGGTGTTCGG
CCAGGACGTCGGTTATTTTGGCGGCGTGTTCCGCTGCACCGACGGCCTGCAGGCCAAGTACGGCCGCTCGCGTGTGTTCG
ACGCGCCTATCTCCGAAGGCGGCATCGTCGGTGCGGCGGTCGGCATGGCCGCCTATGGCTTGCGCCCGGTGGTCGAGATC
CAGTTCGCAGACTACTTTTATCCGGCCTCCGACCAGATCGTGTCCGAAGCGGCGCGGCTGCGTTACCGTTCGGCCGCTGA
CTTCACGGCGCCCATGACCATCCGCATGCCGTGCGGCGGCGGCATCTACGGCGGGCAGACGCACAGCCAAAGCCCGGAGG
CCCTGTTCACCCATGTCTGCGGGCTGCGCACGGTGATGCCGTCCAACCCTTACGACGCCAAGGGTCTGCTGATTGCGTGC
ATCGAGAACGACGATCCGGTGATTTTTCTGGAGCCCAAGCGCCTGTACAACGGGCCTTTCGATGGCCACCATGACAGGCC
GGCGGTACCCTGGACCGGCCACCCGCTGGGCGAGGTGCCCGAGGGTTACTACACCGTGCCGCTGGAGTCGGCCACGGTGT
TCCGCCCCGGCGCCGACCTGACGGTGCTCACCTACGGCACCATGGTCTTCGTCTCGCAGGCGGCGGCGCAGGAGAGCGGC
ATTGACGCCGAGATCATCGACCTGCGCAGCCTCTGGCCGATGGATCTGCAGACCGTGGTGGACTCGGTGAAAAAGACCGG
CCGCTGCGTGGTGGTGCATGAGGCCACGCGCACCAGCGGCTTCGGCGCCGAACTGGCGGCGCTGGTGCAGGAGCACTGCT
TCTACCACCTCGAAGCGCCCATCGAACGCGTGACCGGCTGGGACACGCCTTACCCGCATGCGCAGGAATGGGCCTATTTC
CCCGGCCCGGCGCGCGTGGGCGCGGCGTTCAAGCGCGCGATGGAGGCATGA

Upstream 100 bases:

>100_bases
TGTTCGAGGATGTCTACAAGGACATGCCGGAGCATCTGCGCCGGCAGCGCGAACAACTGCTCAGGGGTGAAATGGACACC
CCCAGGCGGGAGGCCTGACC

Downstream 100 bases:

>100_bases
AATGAGCCCCCACGCTCACATTCGTTCGCTGGACGAACCGCTGCGAGACGCCGCGGCTCTTCGGCCTGTCCAAGCCGGCG
CCAGCCGGCTTGGAGCCGCA

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MQMTMIQALRSAMDVMLERDSNVVVFGQDVGYFGGVFRCTDGLQAKYGRSRVFDAPISEGGIVGAAVGMAAYGLRPVVEI
QFADYFYPASDQIVSEAARLRYRSAADFTAPMTIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIAC
IENDDPVIFLEPKRLYNGPFDGHHDRPAVPWTGHPLGEVPEGYYTVPLESATVFRPGADLTVLTYGTMVFVSQAAAQESG
IDAEIIDLRSLWPMDLQTVVDSVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAPIERVTGWDTPYPHAQEWAYF
PGPARVGAAFKRAMEA

Sequences:

>Translated_336_residues
MQMTMIQALRSAMDVMLERDSNVVVFGQDVGYFGGVFRCTDGLQAKYGRSRVFDAPISEGGIVGAAVGMAAYGLRPVVEI
QFADYFYPASDQIVSEAARLRYRSAADFTAPMTIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIAC
IENDDPVIFLEPKRLYNGPFDGHHDRPAVPWTGHPLGEVPEGYYTVPLESATVFRPGADLTVLTYGTMVFVSQAAAQESG
IDAEIIDLRSLWPMDLQTVVDSVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAPIERVTGWDTPYPHAQEWAYF
PGPARVGAAFKRAMEA
>Mature_336_residues
MQMTMIQALRSAMDVMLERDSNVVVFGQDVGYFGGVFRCTDGLQAKYGRSRVFDAPISEGGIVGAAVGMAAYGLRPVVEI
QFADYFYPASDQIVSEAARLRYRSAADFTAPMTIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIAC
IENDDPVIFLEPKRLYNGPFDGHHDRPAVPWTGHPLGEVPEGYYTVPLESATVFRPGADLTVLTYGTMVFVSQAAAQESG
IDAEIIDLRSLWPMDLQTVVDSVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAPIERVTGWDTPYPHAQEWAYF
PGPARVGAAFKRAMEA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=339, Percent_Identity=45.1327433628319, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI34101272, Length=339, Percent_Identity=45.1327433628319, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI156564403, Length=319, Percent_Identity=34.7962382445141, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI291084858, Length=319, Percent_Identity=33.5423197492163, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=46.0176991150442, Blast_Score=281, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17538422, Length=340, Percent_Identity=37.9411764705882, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=36.1963190184049, Blast_Score=202, Evalue=9e-53,
Organism=Drosophila melanogaster, GI160714832, Length=335, Percent_Identity=47.4626865671642, Blast_Score=303, Evalue=8e-83,
Organism=Drosophila melanogaster, GI160714828, Length=335, Percent_Identity=47.4626865671642, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI21358145, Length=319, Percent_Identity=37.6175548589342, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650940, Length=319, Percent_Identity=37.6175548589342, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=44.4444444444444, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=44.4444444444444, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI160714826, Length=66, Percent_Identity=46.969696969697, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36685; Mature: 36685

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTMIQALRSAMDVMLERDSNVVVFGQDVGYFGGVFRCTDGLQAKYGRSRVFDAPISEG
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCEEECCCHHHHCCCCEEEECCCCCC
GIVGAAVGMAAYGLRPVVEIQFADYFYPASDQIVSEAARLRYRSAADFTAPMTIRMPCGG
CCHHHHHHHHHHCCCEEEEEEEECEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
GIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYNGPF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHCCCC
DGHHDRPAVPWTGHPLGEVPEGYYTVPLESATVFRPGADLTVLTYGTMVFVSQAAAQESG
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHHHHHCC
IDAEIIDLRSLWPMDLQTVVDSVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAP
CCEEEEEHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCCC
IERVTGWDTPYPHAQEWAYFPGPARVGAAFKRAMEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQMTMIQALRSAMDVMLERDSNVVVFGQDVGYFGGVFRCTDGLQAKYGRSRVFDAPISEG
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCEEECCCHHHHCCCCEEEECCCCCC
GIVGAAVGMAAYGLRPVVEIQFADYFYPASDQIVSEAARLRYRSAADFTAPMTIRMPCGG
CCHHHHHHHHHHCCCEEEEEEEECEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
GIYGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYNGPF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHCCCC
DGHHDRPAVPWTGHPLGEVPEGYYTVPLESATVFRPGADLTVLTYGTMVFVSQAAAQESG
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHHHHHCC
IDAEIIDLRSLWPMDLQTVVDSVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAP
CCEEEEEHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCCC
IERVTGWDTPYPHAQEWAYFPGPARVGAAFKRAMEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]