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The map label for this gene is bkdA1 [H]

Identifier: 91786184

GI number: 91786184

Start: 288986

End: 290251

Strand: Direct

Name: bkdA1 [H]

Synonym: Bpro_0273

Alternate gene names: 91786184

Gene position: 288986-290251 (Clockwise)

Preceding gene: 91786182

Following gene: 91786185

Centisome position: 5.56

GC content: 66.67

Gene sequence:

>1266_bases
ATGAACAAGAACCAGGTGCTACGTCTGCATGTGCCGGAGCCTTCGGGGCGCCCCGGCCACGAGACCGACTTTTCCTATCT
GCATCTGTCACCGGCCGGCGCGGTGCGGCGCCCGCCCGTGGAGGCCTCCCCCGCCGACACCAGCGACCTGGCCTACAGCC
TGGTGCGGGTGCTGGATGACGACAACCGCGCCGTGGGGCCCTGGGTTCCGCCGGTGGAACCGCAGCGGCTGCGCCAGGGT
CTGCGCGCCATGATGAAGACGCGCATCTATGACGCCCGCATGCTGATCGCGCAGCGGCAGAAAAAAATCTCGTTTTACAT
GCAGTGCCTGGGCGAGGAGGCGATTGCCACCGCGCATGCGCTGGCGCTGCAGCCCGGCGATATGTGTTTTCCCACCTACC
GCCAGCAGGGGCTGCTGCTGGCGCGCGACGACGTCGACATGGTCGAGATGATCTGCCAGCTGATGTCCAACGAGCGCGAT
CCGCTCAAGGGGAGGCAGCTGCCGGTGATGTACTCGTACAAGCGGGCCGGATTTTTTACCATTTCGGGAAACCTGGCCAC
GCAGTTCATCCAGGCGGTGGGCTGGGGCATGGCGTCGGCGATCAAGGGCGACTCTAAAATAGCCTCGGCCTGGATTGGCG
ATGGCGCCACCGCCGAGGCCGACTTCCATACCGCGCTGACTTTCGCCCATGTGTACCGCGCGCCGGTCATTCTCAACGTG
GTCAACAACCAGTGGGCGATCTCCACCTGCCAGGCGATTGCCGGCGGCGAGGCCACCACTTTTGCTGCGCGGGGTGTCGG
CTGCGGTATTGCCTCCCTGCGCGTGGACGGCAATGACTTTCTCGCTGTCTACGCCGCTTCGCTGTGGGCCGCGGAGCGCG
CGCGCAGCAACCTGGGGCCGACGCTGATCGAGTGGGTCACCTACCGCGGCGGGGCGCACTCGACCTCGGACGATCCCTCC
CGCTACCGCCCCGTCGACGATTGGGCGCACTTTCCGCTGGGCGACCCGATCGCGCGGCTCAAGGCACACCTGATGCAGAC
AGGCGCATGGACCGAGCAGGAGCATGAAGCCACGCAGAAGGAGCTGGAGGCCCAGGTGAGCGCGGCGCTGAAGGAGGCCG
AGCGCTACGGCTCGCTGGCCGACGGACACCTGGCCGGCGCGGCGGCGATGTTCGAGGATGTCTACAAGGACATGCCGGAG
CATCTGCGCCGGCAGCGCGAACAACTGCTCAGGGGTGAAATGGACACCCCCAGGCGGGAGGCCTGA

Upstream 100 bases:

>100_bases
AATGGCCGCATCCCCCAAAAGGATGAGCGGAGGGGGCAAGCCCAGTCGCTGGTTTGCACCTTAAGCGGGTCAGGGCTACG
ATGTGTCAGGGGTGACGCGG

Downstream 100 bases:

>100_bases
CCATGCAGATGACCATGATCCAGGCACTGCGCTCGGCGATGGACGTGATGCTGGAACGTGACAGCAATGTCGTGGTGTTC
GGCCAGGACGTCGGTTATTT

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNKNQVLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVEASPADTSDLAYSLVRVLDDDNRAVGPWVPPVEPQRLRQG
LRAMMKTRIYDARMLIAQRQKKISFYMQCLGEEAIATAHALALQPGDMCFPTYRQQGLLLARDDVDMVEMICQLMSNERD
PLKGRQLPVMYSYKRAGFFTISGNLATQFIQAVGWGMASAIKGDSKIASAWIGDGATAEADFHTALTFAHVYRAPVILNV
VNNQWAISTCQAIAGGEATTFAARGVGCGIASLRVDGNDFLAVYAASLWAAERARSNLGPTLIEWVTYRGGAHSTSDDPS
RYRPVDDWAHFPLGDPIARLKAHLMQTGAWTEQEHEATQKELEAQVSAALKEAERYGSLADGHLAGAAAMFEDVYKDMPE
HLRRQREQLLRGEMDTPRREA

Sequences:

>Translated_421_residues
MNKNQVLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVEASPADTSDLAYSLVRVLDDDNRAVGPWVPPVEPQRLRQG
LRAMMKTRIYDARMLIAQRQKKISFYMQCLGEEAIATAHALALQPGDMCFPTYRQQGLLLARDDVDMVEMICQLMSNERD
PLKGRQLPVMYSYKRAGFFTISGNLATQFIQAVGWGMASAIKGDSKIASAWIGDGATAEADFHTALTFAHVYRAPVILNV
VNNQWAISTCQAIAGGEATTFAARGVGCGIASLRVDGNDFLAVYAASLWAAERARSNLGPTLIEWVTYRGGAHSTSDDPS
RYRPVDDWAHFPLGDPIARLKAHLMQTGAWTEQEHEATQKELEAQVSAALKEAERYGSLADGHLAGAAAMFEDVYKDMPE
HLRRQREQLLRGEMDTPRREA
>Mature_421_residues
MNKNQVLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVEASPADTSDLAYSLVRVLDDDNRAVGPWVPPVEPQRLRQG
LRAMMKTRIYDARMLIAQRQKKISFYMQCLGEEAIATAHALALQPGDMCFPTYRQQGLLLARDDVDMVEMICQLMSNERD
PLKGRQLPVMYSYKRAGFFTISGNLATQFIQAVGWGMASAIKGDSKIASAWIGDGATAEADFHTALTFAHVYRAPVILNV
VNNQWAISTCQAIAGGEATTFAARGVGCGIASLRVDGNDFLAVYAASLWAAERARSNLGPTLIEWVTYRGGAHSTSDDPS
RYRPVDDWAHFPLGDPIARLKAHLMQTGAWTEQEHEATQKELEAQVSAALKEAERYGSLADGHLAGAAAMFEDVYKDMPE
HLRRQREQLLRGEMDTPRREA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=360, Percent_Identity=38.3333333333333, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI258645172, Length=361, Percent_Identity=38.2271468144044, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI4885543, Length=315, Percent_Identity=26.984126984127, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4505685, Length=315, Percent_Identity=26.984126984127, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI291084742, Length=315, Percent_Identity=26.984126984127, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI291084744, Length=270, Percent_Identity=26.2962962962963, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI86563357, Length=343, Percent_Identity=38.7755102040816, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI86563355, Length=343, Percent_Identity=38.7755102040816, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17536047, Length=308, Percent_Identity=29.2207792207792, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32564172, Length=308, Percent_Identity=29.2207792207792, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321026, Length=341, Percent_Identity=29.3255131964809, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355903, Length=362, Percent_Identity=33.7016574585635, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24639748, Length=267, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24639744, Length=292, Percent_Identity=26.7123287671233, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28571106, Length=292, Percent_Identity=26.7123287671233, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24639740, Length=292, Percent_Identity=26.7123287671233, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24639746, Length=292, Percent_Identity=26.7123287671233, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 46508; Mature: 46508

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNQVLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVEASPADTSDLAYSLVRVLDD
CCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
DNRAVGPWVPPVEPQRLRQGLRAMMKTRIYDARMLIAQRQKKISFYMQCLGEEAIATAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALQPGDMCFPTYRQQGLLLARDDVDMVEMICQLMSNERDPLKGRQLPVMYSYKRAGFFT
HEECCCCCCCHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEE
ISGNLATQFIQAVGWGMASAIKGDSKIASAWIGDGATAEADFHTALTFAHVYRAPVILNV
EECHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
VNNQWAISTCQAIAGGEATTFAARGVGCGIASLRVDGNDFLAVYAASLWAAERARSNLGP
CCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCH
TLIEWVTYRGGAHSTSDDPSRYRPVDDWAHFPLGDPIARLKAHLMQTGAWTEQEHEATQK
HHHHHHHHCCCCCCCCCCCHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ELEAQVSAALKEAERYGSLADGHLAGAAAMFEDVYKDMPEHLRRQREQLLRGEMDTPRRE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
A
C
>Mature Secondary Structure
MNKNQVLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVEASPADTSDLAYSLVRVLDD
CCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
DNRAVGPWVPPVEPQRLRQGLRAMMKTRIYDARMLIAQRQKKISFYMQCLGEEAIATAHA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALQPGDMCFPTYRQQGLLLARDDVDMVEMICQLMSNERDPLKGRQLPVMYSYKRAGFFT
HEECCCCCCCHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEE
ISGNLATQFIQAVGWGMASAIKGDSKIASAWIGDGATAEADFHTALTFAHVYRAPVILNV
EECHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
VNNQWAISTCQAIAGGEATTFAARGVGCGIASLRVDGNDFLAVYAASLWAAERARSNLGP
CCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCH
TLIEWVTYRGGAHSTSDDPSRYRPVDDWAHFPLGDPIARLKAHLMQTGAWTEQEHEATQK
HHHHHHHHCCCCCCCCCCCHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ELEAQVSAALKEAERYGSLADGHLAGAAAMFEDVYKDMPEHLRRQREQLLRGEMDTPRRE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]