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The map label for this gene is bkdB [H]

Identifier: 91786186

GI number: 91786186

Start: 291421

End: 292698

Strand: Direct

Name: bkdB [H]

Synonym: Bpro_0275

Alternate gene names: 91786186

Gene position: 291421-292698 (Clockwise)

Preceding gene: 91786185

Following gene: 91786191

Centisome position: 5.6

GC content: 68.08

Gene sequence:

>1278_bases
ATGAGCATTCACATCATCAAAATGCCCGACCTCGGCGAGGGCATCACCGAGGTCGAGCTGGTGGCCTGGCGCGTCAAGCC
CGGCGACCGTGTGACCGAAGACCAGGTGCTGGCCGATGTGATGACCGACAAGGCCACCGTCGAGATCCCGTCGCCTGTGG
TGGGCCAGGTGCTGGCCCTGGGCGGCGAGGTCGGCCAGGTGCTGGCAGTGGGGGCGGAGCTGATCCGCATTGAAGTGGAA
GGAGCGGGTGCCGCCAGCGAGGCTGCTCCATCCGTTTTGACCGTGCCGCAAGACGCGACCGCATCCATGCCGGTGGTCCC
TGCGCCCGCACCGGCATCGACCCTGACATCGATCCCGACTTCGATCCCGGACGCAATCGCTCCCCCAAGCCCCTCCGCTG
ACAAGCCTATTGCTTCGCCGGCCGTGCGTCGGCGCGCCTGGGAGCTTGGCATTGACTTGCAGCAGGTCGCCGCCAGCGGT
GCGGGTGGCCGCATCATGCAGGCCGACCTCGATGCCCACGTGGCGGCGCATGGGACGGCCCCGCCGGCCGTTGCGGGTTC
AACGGGTCTTGCGCAGCGCAACGACGAAGAAAAGGTGCCGGTGATTGGCCTGCGCCGCCGCATCGCGCAGAAGATGCAGG
AGTCCAAGCGCCGCATCCCGCATTTCACCTATGTCGAAGAGGTCGACGTCACCGAGCTGGAGGCGTTGCGTGCGCGCCTG
AACGCGAAGTGGGGCGCGCAGCGCGGCCACCTGACACTGCTGCCCCTGCTGGTGCGGGCCGTGGTGCTGGCCGTGCGCGA
GTTTCCGCAGGTCAACGCACGTTTTGACGACGAAGCCGGCGTGGTCACGCGCCATGGCGCGGTGCACATTGGCATTGCCA
CCCAGACCGGGGCCGGTCTCATGGTGCCGGTGCTGCGCCATGCCGAGGCCAGAGACCTGTGGTCCAGCGCGGCAGAGGTC
GTGCGCCTGGCCGAAGCCGCGCGCGCCGGCAAGGCCACACGCGACGAACTCACCGGTGCGACGCTGACCCTGACCAGCCT
GGGCGCCCTGGGCGGCATCGTCTCGACACCGGTGATCAACCATCCCGAAGTGGCCATCGTCGGTGTCAACCGCATCGTGG
AGCGGCCGGTGATGCGCGACGGCGCGGTGGTGGCGCGGCAGATGATGAACCTGTCGTCGTCGTTTGACCACCGCGTGGTC
GATGGCGTGGATGCGGCTGGTTTCGTGCAGGCTGTGCGCGGTTACCTCGAATCCCCGGTCACGCTGTTCGTGGAGTAG

Upstream 100 bases:

>100_bases
CTCTTCGGCCTGTCCAAGCCGGCGCCAGCCGGCTTGGAGCCGCAGGCCTCAGCCCCTCAGGGGCGCGCCTCCCTAGGGGC
GGCCCGGCGGGAGGCATGGC

Downstream 100 bases:

>100_bases
GCCCTATTGGGAAGTGGGAAGTGGGAAGTCGCCCGATGACCTGCACATGATCATTGGTGAAGAAGAAAGGAAAGTCCGCC
AGCACCCGGCGGACACCGGC

Product: branched-chain alpha-keto acid dehydrogenase E2 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSIHIIKMPDLGEGITEVELVAWRVKPGDRVTEDQVLADVMTDKATVEIPSPVVGQVLALGGEVGQVLAVGAELIRIEVE
GAGAASEAAPSVLTVPQDATASMPVVPAPAPASTLTSIPTSIPDAIAPPSPSADKPIASPAVRRRAWELGIDLQQVAASG
AGGRIMQADLDAHVAAHGTAPPAVAGSTGLAQRNDEEKVPVIGLRRRIAQKMQESKRRIPHFTYVEEVDVTELEALRARL
NAKWGAQRGHLTLLPLLVRAVVLAVREFPQVNARFDDEAGVVTRHGAVHIGIATQTGAGLMVPVLRHAEARDLWSSAAEV
VRLAEAARAGKATRDELTGATLTLTSLGALGGIVSTPVINHPEVAIVGVNRIVERPVMRDGAVVARQMMNLSSSFDHRVV
DGVDAAGFVQAVRGYLESPVTLFVE

Sequences:

>Translated_425_residues
MSIHIIKMPDLGEGITEVELVAWRVKPGDRVTEDQVLADVMTDKATVEIPSPVVGQVLALGGEVGQVLAVGAELIRIEVE
GAGAASEAAPSVLTVPQDATASMPVVPAPAPASTLTSIPTSIPDAIAPPSPSADKPIASPAVRRRAWELGIDLQQVAASG
AGGRIMQADLDAHVAAHGTAPPAVAGSTGLAQRNDEEKVPVIGLRRRIAQKMQESKRRIPHFTYVEEVDVTELEALRARL
NAKWGAQRGHLTLLPLLVRAVVLAVREFPQVNARFDDEAGVVTRHGAVHIGIATQTGAGLMVPVLRHAEARDLWSSAAEV
VRLAEAARAGKATRDELTGATLTLTSLGALGGIVSTPVINHPEVAIVGVNRIVERPVMRDGAVVARQMMNLSSSFDHRVV
DGVDAAGFVQAVRGYLESPVTLFVE
>Mature_424_residues
SIHIIKMPDLGEGITEVELVAWRVKPGDRVTEDQVLADVMTDKATVEIPSPVVGQVLALGGEVGQVLAVGAELIRIEVEG
AGAASEAAPSVLTVPQDATASMPVVPAPAPASTLTSIPTSIPDAIAPPSPSADKPIASPAVRRRAWELGIDLQQVAASGA
GGRIMQADLDAHVAAHGTAPPAVAGSTGLAQRNDEEKVPVIGLRRRIAQKMQESKRRIPHFTYVEEVDVTELEALRARLN
AKWGAQRGHLTLLPLLVRAVVLAVREFPQVNARFDDEAGVVTRHGAVHIGIATQTGAGLMVPVLRHAEARDLWSSAAEVV
RLAEAARAGKATRDELTGATLTLTSLGALGGIVSTPVINHPEVAIVGVNRIVERPVMRDGAVVARQMMNLSSSFDHRVVD
GVDAAGFVQAVRGYLESPVTLFVE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=30.2483069977427, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=29.2906178489703, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=31.1740890688259, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI203098816, Length=232, Percent_Identity=27.5862068965517, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI203098753, Length=232, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=29.585798816568, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=30.9302325581395, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=30.7511737089202, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=30.9633027522936, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=30.0715990453461, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=28.7015945330296, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=24.6794871794872, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=29.1375291375291, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=25.5411255411255, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=28.5714285714286, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24582497, Length=418, Percent_Identity=28.2296650717703, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645909, Length=243, Percent_Identity=30.4526748971193, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=28.82096069869, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 44658; Mature: 44527

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHIIKMPDLGEGITEVELVAWRVKPGDRVTEDQVLADVMTDKATVEIPSPVVGQVLAL
CEEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHH
GGEVGQVLAVGAELIRIEVEGAGAASEAAPSVLTVPQDATASMPVVPAPAPASTLTSIPT
CCHHHHHHHHCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCC
SIPDAIAPPSPSADKPIASPAVRRRAWELGIDLQQVAASGAGGRIMQADLDAHVAAHGTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHEEECCCC
PPAVAGSTGLAQRNDEEKVPVIGLRRRIAQKMQESKRRIPHFTYVEEVDVTELEALRARL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
NAKWGAQRGHLTLLPLLVRAVVLAVREFPQVNARFDDEAGVVTRHGAVHIGIATQTGAGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCH
MVPVLRHAEARDLWSSAAEVVRLAEAARAGKATRDELTGATLTLTSLGALGGIVSTPVIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEHHHHHHHHHHHHCCCCC
HPEVAIVGVNRIVERPVMRDGAVVARQMMNLSSSFDHRVVDGVDAAGFVQAVRGYLESPV
CCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCE
TLFVE
EEEEC
>Mature Secondary Structure 
SIHIIKMPDLGEGITEVELVAWRVKPGDRVTEDQVLADVMTDKATVEIPSPVVGQVLAL
EEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHH
GGEVGQVLAVGAELIRIEVEGAGAASEAAPSVLTVPQDATASMPVVPAPAPASTLTSIPT
CCHHHHHHHHCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCC
SIPDAIAPPSPSADKPIASPAVRRRAWELGIDLQQVAASGAGGRIMQADLDAHVAAHGTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHEEECCCC
PPAVAGSTGLAQRNDEEKVPVIGLRRRIAQKMQESKRRIPHFTYVEEVDVTELEALRARL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
NAKWGAQRGHLTLLPLLVRAVVLAVREFPQVNARFDDEAGVVTRHGAVHIGIATQTGAGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCH
MVPVLRHAEARDLWSSAAEVVRLAEAARAGKATRDELTGATLTLTSLGALGGIVSTPVIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEHHHHHHHHHHHHCCCCC
HPEVAIVGVNRIVERPVMRDGAVVARQMMNLSSSFDHRVVDGVDAAGFVQAVRGYLESPV
CCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCE
TLFVE
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]