Click here to switch to the map view.

The map label for this gene is alkJ [H]

Identifier: 91786140

GI number: 91786140

Start: 250832

End: 252574

Strand: Reverse

Name: alkJ [H]

Synonym: Bpro_0229

Alternate gene names: 91786140

Gene position: 252574-250832 (Counterclockwise)

Preceding gene: 91786147

Following gene: 91786139

Centisome position: 4.86

GC content: 65.81

Gene sequence:

>1743_bases
ATGCAAGACGAGAACCAGGTTTTTGACTACATCATCATTGGCGCCGGCACGGCCGGTTGTCTGCTGGCCAACCGCCTCAG
CGCTGACGCTTCCAAGCGCGTGCTGCTGATCGAGGCCGGCCGCAAGGACGACTACCACTGGATCCACATTCCGGTGGGCT
ATCTGCACTGCATTGGCAACCCGCGCACCGACTGGCTGTACAACACCGAGCCGGACGCCGGCCTCAACGGCCGCGCACTG
CGCTACCCGCGGGGCAAAACGCTGGGCGGCTGCTCGAGCATCAACGGCATGATTTACATGCGCGGCCAGGCCCGCGACTA
CGACCGGTGGGCGGAACTGACCGGCGACAGCGCCTGGCGCTGGGACAACGCCCTGCCTCATTTCAAGCTGCACGAAGACT
ACTACAAGGGCGCGGACGCCATGCACGGCGCACGCGGCACCGCCCCCGAGCTGATGCAGGACAAGCTCAACCCCTACCAG
AAGCTGCTGCGCCACCGCAACGCGGGCGGCGAATGGCGCATTGAAAAACAGCGGCTGCGCTGGGACATTCTGGATGCGTT
TGCCGAGGCTGCTACCCAAGCCGGCATTCCGGCCACCCCGGACTTCAACCGGGGCGACAACGAAGGCGTCGGGTATTTTG
AAGTCAACCAGAAAAGCGGCTGGCGCTGGAATACCGCCAAGGCCTTCCTGCGGCCGGCCTGCTACGGCCGGCCCAATTTT
GAGCTCTGGACCAATGCGCAGGTGTGCAAGCTGCTGATCGAGCCGCAGCCCGATGGCAGCCAGCGCTGTACCGGCGCCGA
GGTATGGACCGGCCAGGGACGCATCACCGCGCTGGCCACCCGGGACTCGGAACACATGGGCGAGGTCATTCTCTGCGCCG
GCAGCATCGGCTCGCCGCAAATTCTGCAGCTCTCGGGCATTGGCCCGGCCGCCCTGCTGCAGCAGCACGGCATCCCCGTG
GTGCAGGATCTGCCCGGTGTCGGCGCCAACCTGCAGGACCACCTGCAAATTCGCTCGGTCTACAAGGTGCAGGCCCTGGA
AGGCGGCAAGCGCTGGGGCCTGTCGCTCAACACCATGGCCAATTCGCTGTGGGGCAAGGCCCGCATCGGGCTCGAATATG
CGTTTCGCCAGACCGGCCCGATGAGCATGGCGCCCTCGCAACTGGGCGCCTTCACGCGCAGCTCGCCGGCGCAACCCTAC
CCGAACATCGAGTACCACGTCCAGCCGCTGTCGCTCGATGCCTTTGGCGAGCCGCTGCACAATTTCAATGCCTTCACGGC
GAGCGTGTGCAACCTCAACCCGACCAGCCGCGGCACCGTGCACATCAAGAGCGGCCATTTTGAGGACGCCCCGGCCATCG
CCCCCAATTACCTAAGCACGGCCGAGGACCGCCAGGTGGCGGCCGACTCGCTGCGCGTGACGCGCCGCATTGTCAGCCAG
AGCGCGCTGGCCAAATACCGGCCTGAAGAATTCAAGCCGGGCGTGCAGTTCCAGACCGACGAAGAACTCACGCGGCTGGC
CGGCGACATTGCCACCACCATCTTCCACCCGGTCGGCACCACCAAAATGGGCCGCCATGACGACCCCCTCGCGGTGGTGG
ACTCGCACCTGCGGGTGCGCGGCGTTCGCGGCCTGCGCGTCGTCGATGCCGGGGTGATGCCGCTGATCACCAGCGGAAAT
ACCAACTCGCCCACGCTGATGATCGCGGAGAAGGCGGCGCAGTGGATTCGCGACGGCGAGTAA

Upstream 100 bases:

>100_bases
TCCTGCGTTAGGCAAGGCAGGCAGGATCCCCCTGCTACCCCGCCCAAAACCAGTCACCATCACAGCCCCGGCCGGGAACC
GCAAGACAAAAAGCACACCC

Downstream 100 bases:

>100_bases
GCCCGCCGGGGATGCCGGGATCGGCATCGCCGCTTTGTTCCTTCGGTGGCGTTCACGCGCAATAATGGGCATCAGCCCCC
CATTCCCACTGAGCCGCCAG

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MQDENQVFDYIIIGAGTAGCLLANRLSADASKRVLLIEAGRKDDYHWIHIPVGYLHCIGNPRTDWLYNTEPDAGLNGRAL
RYPRGKTLGGCSSINGMIYMRGQARDYDRWAELTGDSAWRWDNALPHFKLHEDYYKGADAMHGARGTAPELMQDKLNPYQ
KLLRHRNAGGEWRIEKQRLRWDILDAFAEAATQAGIPATPDFNRGDNEGVGYFEVNQKSGWRWNTAKAFLRPACYGRPNF
ELWTNAQVCKLLIEPQPDGSQRCTGAEVWTGQGRITALATRDSEHMGEVILCAGSIGSPQILQLSGIGPAALLQQHGIPV
VQDLPGVGANLQDHLQIRSVYKVQALEGGKRWGLSLNTMANSLWGKARIGLEYAFRQTGPMSMAPSQLGAFTRSSPAQPY
PNIEYHVQPLSLDAFGEPLHNFNAFTASVCNLNPTSRGTVHIKSGHFEDAPAIAPNYLSTAEDRQVAADSLRVTRRIVSQ
SALAKYRPEEFKPGVQFQTDEELTRLAGDIATTIFHPVGTTKMGRHDDPLAVVDSHLRVRGVRGLRVVDAGVMPLITSGN
TNSPTLMIAEKAAQWIRDGE

Sequences:

>Translated_580_residues
MQDENQVFDYIIIGAGTAGCLLANRLSADASKRVLLIEAGRKDDYHWIHIPVGYLHCIGNPRTDWLYNTEPDAGLNGRAL
RYPRGKTLGGCSSINGMIYMRGQARDYDRWAELTGDSAWRWDNALPHFKLHEDYYKGADAMHGARGTAPELMQDKLNPYQ
KLLRHRNAGGEWRIEKQRLRWDILDAFAEAATQAGIPATPDFNRGDNEGVGYFEVNQKSGWRWNTAKAFLRPACYGRPNF
ELWTNAQVCKLLIEPQPDGSQRCTGAEVWTGQGRITALATRDSEHMGEVILCAGSIGSPQILQLSGIGPAALLQQHGIPV
VQDLPGVGANLQDHLQIRSVYKVQALEGGKRWGLSLNTMANSLWGKARIGLEYAFRQTGPMSMAPSQLGAFTRSSPAQPY
PNIEYHVQPLSLDAFGEPLHNFNAFTASVCNLNPTSRGTVHIKSGHFEDAPAIAPNYLSTAEDRQVAADSLRVTRRIVSQ
SALAKYRPEEFKPGVQFQTDEELTRLAGDIATTIFHPVGTTKMGRHDDPLAVVDSHLRVRGVRGLRVVDAGVMPLITSGN
TNSPTLMIAEKAAQWIRDGE
>Mature_580_residues
MQDENQVFDYIIIGAGTAGCLLANRLSADASKRVLLIEAGRKDDYHWIHIPVGYLHCIGNPRTDWLYNTEPDAGLNGRAL
RYPRGKTLGGCSSINGMIYMRGQARDYDRWAELTGDSAWRWDNALPHFKLHEDYYKGADAMHGARGTAPELMQDKLNPYQ
KLLRHRNAGGEWRIEKQRLRWDILDAFAEAATQAGIPATPDFNRGDNEGVGYFEVNQKSGWRWNTAKAFLRPACYGRPNF
ELWTNAQVCKLLIEPQPDGSQRCTGAEVWTGQGRITALATRDSEHMGEVILCAGSIGSPQILQLSGIGPAALLQQHGIPV
VQDLPGVGANLQDHLQIRSVYKVQALEGGKRWGLSLNTMANSLWGKARIGLEYAFRQTGPMSMAPSQLGAFTRSSPAQPY
PNIEYHVQPLSLDAFGEPLHNFNAFTASVCNLNPTSRGTVHIKSGHFEDAPAIAPNYLSTAEDRQVAADSLRVTRRIVSQ
SALAKYRPEEFKPGVQFQTDEELTRLAGDIATTIFHPVGTTKMGRHDDPLAVVDSHLRVRGVRGLRVVDAGVMPLITSGN
TNSPTLMIAEKAAQWIRDGE

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=577, Percent_Identity=38.6481802426343, Blast_Score=361, Evalue=1e-99,
Organism=Escherichia coli, GI1786503, Length=581, Percent_Identity=38.382099827883, Blast_Score=345, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17532301, Length=579, Percent_Identity=33.3333333333333, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24642042, Length=607, Percent_Identity=33.6079077429984, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24642055, Length=612, Percent_Identity=34.4771241830065, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24642048, Length=617, Percent_Identity=31.9286871961102, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI45549471, Length=608, Percent_Identity=31.25, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI45551458, Length=608, Percent_Identity=31.25, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24642059, Length=608, Percent_Identity=32.2368421052632, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI18859995, Length=603, Percent_Identity=32.8358208955224, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24650267, Length=605, Percent_Identity=31.7355371900826, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17137792, Length=590, Percent_Identity=32.0338983050847, Blast_Score=239, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24642039, Length=595, Percent_Identity=31.5966386554622, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI18859993, Length=612, Percent_Identity=30.718954248366, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24642035, Length=620, Percent_Identity=30, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24642051, Length=611, Percent_Identity=28.8052373158756, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24642037, Length=609, Percent_Identity=29.2282430213465, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24645930, Length=618, Percent_Identity=27.5080906148867, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24642057, Length=623, Percent_Identity=26.645264847512, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 64047; Mature: 64047

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDENQVFDYIIIGAGTAGCLLANRLSADASKRVLLIEAGRKDDYHWIHIPVGYLHCIGN
CCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECEEEEECCC
PRTDWLYNTEPDAGLNGRALRYPRGKTLGGCSSINGMIYMRGQARDYDRWAELTGDSAWR
CCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCC
WDNALPHFKLHEDYYKGADAMHGARGTAPELMQDKLNPYQKLLRHRNAGGEWRIEKQRLR
CCCCCCCEEECHHHHCCCHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEEEEHHHHH
WDILDAFAEAATQAGIPATPDFNRGDNEGVGYFEVNQKSGWRWNTAKAFLRPACYGRPNF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHHHHHCCCCCC
ELWTNAQVCKLLIEPQPDGSQRCTGAEVWTGQGRITALATRDSEHMGEVILCAGSIGSPQ
EEECCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEECCCHHCCCEEEEECCCCCCC
ILQLSGIGPAALLQQHGIPVVQDLPGVGANLQDHLQIRSVYKVQALEGGKRWGLSLNTMA
EEEECCCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCEEHHHHH
NSLWGKARIGLEYAFRQTGPMSMAPSQLGAFTRSSPAQPYPNIEYHVQPLSLDAFGEPLH
HHHHCHHHCCEEEHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCHH
NFNAFTASVCNLNPTSRGTVHIKSGHFEDAPAIAPNYLSTAEDRQVAADSLRVTRRIVSQ
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SALAKYRPEEFKPGVQFQTDEELTRLAGDIATTIFHPVGTTKMGRHDDPLAVVDSHLRVR
HHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEE
GVRGLRVVDAGVMPLITSGNTNSPTLMIAEKAAQWIRDGE
CCCCEEEECCCCEEEEECCCCCCCEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MQDENQVFDYIIIGAGTAGCLLANRLSADASKRVLLIEAGRKDDYHWIHIPVGYLHCIGN
CCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECEEEEECCC
PRTDWLYNTEPDAGLNGRALRYPRGKTLGGCSSINGMIYMRGQARDYDRWAELTGDSAWR
CCCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCC
WDNALPHFKLHEDYYKGADAMHGARGTAPELMQDKLNPYQKLLRHRNAGGEWRIEKQRLR
CCCCCCCEEECHHHHCCCHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEEEEHHHHH
WDILDAFAEAATQAGIPATPDFNRGDNEGVGYFEVNQKSGWRWNTAKAFLRPACYGRPNF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHHHHHCCCCCC
ELWTNAQVCKLLIEPQPDGSQRCTGAEVWTGQGRITALATRDSEHMGEVILCAGSIGSPQ
EEECCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEECCCHHCCCEEEEECCCCCCC
ILQLSGIGPAALLQQHGIPVVQDLPGVGANLQDHLQIRSVYKVQALEGGKRWGLSLNTMA
EEEECCCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCEEHHHHH
NSLWGKARIGLEYAFRQTGPMSMAPSQLGAFTRSSPAQPYPNIEYHVQPLSLDAFGEPLH
HHHHCHHHCCEEEHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCHH
NFNAFTASVCNLNPTSRGTVHIKSGHFEDAPAIAPNYLSTAEDRQVAADSLRVTRRIVSQ
CCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SALAKYRPEEFKPGVQFQTDEELTRLAGDIATTIFHPVGTTKMGRHDDPLAVVDSHLRVR
HHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEE
GVRGLRVVDAGVMPLITSGNTNSPTLMIAEKAAQWIRDGE
CCCCEEEECCCCEEEEECCCCCCCEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]