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The map label for this gene is pckG [H]

Identifier: 91786071

GI number: 91786071

Start: 171455

End: 173323

Strand: Reverse

Name: pckG [H]

Synonym: Bpro_0160

Alternate gene names: 91786071

Gene position: 173323-171455 (Counterclockwise)

Preceding gene: 91786076

Following gene: 91786069

Centisome position: 3.33

GC content: 63.67

Gene sequence:

>1869_bases
ATGAACCACCCCGTGATGCAAGGACTGGAACTGGGCACGCCCGGCTATGTCAAGAACGCAAAACTGATTGCCTGGGTTGC
CGACATGGTGGCCCTGTGCAAACCCGACCGCGTGTACTGGTGCGACGGCAGCGACGCCGAATACCAGCGCCTGTGCCAGC
AGCTGGTCGATGCCGGCACCTTCAAAAAGCTCAACGAAACCAAGCGCCCCAACAGCTTCCTGGCGCTGAGCGACCCCTCC
GACGTGGCGCGCGTGGAAGACCGCACCTACATCTGCTCGGCCCGCAAGGAAGACGCCGGCCCGACCAACAACTGGATGGC
CCCGGCCGAGATGCGCGCCACGCTGCAGCCGCTGTTTGACGGCTGCATGCAGGGCCGCACCATGTATGTGGTGCCTTTCA
GCATGGGCCCGCTGGGCTCGCCGATTGCACACATCGGCGTCGAGCTGTCGGACAGCCCTTATGTGGCTGTCAACATGAAG
ATCATGACGCGCATGGGCAAGGCGGTGTTCGACGTGCTGGGCGTCAATGGCGACTTCGTGCCCTGCGTACACACCGTGGG
CGCGCCGTTGGCCGCCGGCCAGAAAGACGTGACATGGCCGTGCAACAAGACCAAATACATCGTGCACTACCCGGAGACCC
GCGAAATCTGGTCCTACGGTTCGGGCTACGGCGGCAATGCGCTGCTGGGCAAGAAGTGCTTTGCGCTGCGTATCGCGTCC
AACATGGGCCGCGACGAGGGCTGGCTGGCCGAGCACATGCTGATTCTCGGCGTGACCAACCCTGAGGGCAAGAAATACCA
TGTCGCAGCGGCCTTCCCGTCGGCTTGCGGCAAGACCAACTTCTCCATGCTGGTGCCACCCGCCGGCTTTGACGGCTGGA
AAGTCACCACCATCGGCGACGACATCGCCTGGATCAAGCCCGCGGCCGACGGCAAGCTGTATGCCATCAACCCGGAAGCC
GGCTACTTCGGCGTGGCGCCCGGCACCAACATGCTGACCAACCCGAACTGCATGCGCAGCCTGGACCGCGACACGATTTT
CACCAACGTCGGGCTGACCGACGATGGCGACGTGTGGTGGGAGGGCATGGAGCAGGATGCACCCGGCAAGGCACTGCCGG
CCCACCTGATCGACTGGCAGGGCAATGACTGGACACCCCAGATTGCCAAGGAAACCGGCGCCAAGGCCGCACATCCCAAT
TCACGCTTTACCGTGGCCGCGACCAACAATCCGGCGCTCGACAGCGCCTGGGACGACCCGAAAGGCGTGGCGATCGACGC
CTTCATCTTCGGCGGCCGCCGCTCGACCACCGTGCCGCTGGTGACCGAGGCGCGCAACTGGGTGGAAGGCGTCTACATGG
CCGCCACCATGGGCTCCGAAACCACGGCTGCCGCGACCGGCCAGGCCGGTGTGGTGCGCCGCGACCCGTTCGCCATGCTG
CCGTTTACCGGCTACAACATGAGCGACTACTTCCAGCACTGGCTGGATATGGGTCAGAAGCTCGCCGGTTCCGGCGCCAA
ACTACCGAAAATCTACACCACCAACTGGTTCCGCAAAGGTGCGGATGGCAAATTCGTCTGGCCTGGCTACGGTGAGAACA
TGCGCGTGCTGAAGTGGATGATTGACCGCATCGAAGGCAGCGGCCAGGGCAATGACCACATCACCGGCGTCAGCCCCAGC
TACGAAGACCTGAACTGGACCGGCCTCGACTTCAGCGCCGAGCAGTTCGACAGCGTGATCAGCATGGACAAGGCCGCCTG
GCTCAAGGAGCTGGAGCTGCACGCCGAACTGTTCAAGCAGCTTGAACACCACCTGCCGAAAGAACTGGACCAGACCATGT
CCGCCATCGAGCGCCGGCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCATTTCGACAGACGCCCGATGTCGCAGCAACTGCCGCCGCAATTGCCCACATTCACCGGCGTTTATCGAAATAACGTT
TTTCAACTTTGGAGTTTTCA

Downstream 100 bases:

>100_bases
CATCCGCCCGCGCCCGGGCGCCTTGGCGCCCGGGCTGTCTCTGGCACTTTGACGTTTCGACAAGCTGGAGCCAGACGAAA
AAAAGCCACCTCATGAGGTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNHPVMQGLELGTPGYVKNAKLIAWVADMVALCKPDRVYWCDGSDAEYQRLCQQLVDAGTFKKLNETKRPNSFLALSDPS
DVARVEDRTYICSARKEDAGPTNNWMAPAEMRATLQPLFDGCMQGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMK
IMTRMGKAVFDVLGVNGDFVPCVHTVGAPLAAGQKDVTWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
NMGRDEGWLAEHMLILGVTNPEGKKYHVAAAFPSACGKTNFSMLVPPAGFDGWKVTTIGDDIAWIKPAADGKLYAINPEA
GYFGVAPGTNMLTNPNCMRSLDRDTIFTNVGLTDDGDVWWEGMEQDAPGKALPAHLIDWQGNDWTPQIAKETGAKAAHPN
SRFTVAATNNPALDSAWDDPKGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAATGQAGVVRRDPFAML
PFTGYNMSDYFQHWLDMGQKLAGSGAKLPKIYTTNWFRKGADGKFVWPGYGENMRVLKWMIDRIEGSGQGNDHITGVSPS
YEDLNWTGLDFSAEQFDSVISMDKAAWLKELELHAELFKQLEHHLPKELDQTMSAIERRLAA

Sequences:

>Translated_622_residues
MNHPVMQGLELGTPGYVKNAKLIAWVADMVALCKPDRVYWCDGSDAEYQRLCQQLVDAGTFKKLNETKRPNSFLALSDPS
DVARVEDRTYICSARKEDAGPTNNWMAPAEMRATLQPLFDGCMQGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMK
IMTRMGKAVFDVLGVNGDFVPCVHTVGAPLAAGQKDVTWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
NMGRDEGWLAEHMLILGVTNPEGKKYHVAAAFPSACGKTNFSMLVPPAGFDGWKVTTIGDDIAWIKPAADGKLYAINPEA
GYFGVAPGTNMLTNPNCMRSLDRDTIFTNVGLTDDGDVWWEGMEQDAPGKALPAHLIDWQGNDWTPQIAKETGAKAAHPN
SRFTVAATNNPALDSAWDDPKGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAATGQAGVVRRDPFAML
PFTGYNMSDYFQHWLDMGQKLAGSGAKLPKIYTTNWFRKGADGKFVWPGYGENMRVLKWMIDRIEGSGQGNDHITGVSPS
YEDLNWTGLDFSAEQFDSVISMDKAAWLKELELHAELFKQLEHHLPKELDQTMSAIERRLAA
>Mature_622_residues
MNHPVMQGLELGTPGYVKNAKLIAWVADMVALCKPDRVYWCDGSDAEYQRLCQQLVDAGTFKKLNETKRPNSFLALSDPS
DVARVEDRTYICSARKEDAGPTNNWMAPAEMRATLQPLFDGCMQGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMK
IMTRMGKAVFDVLGVNGDFVPCVHTVGAPLAAGQKDVTWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
NMGRDEGWLAEHMLILGVTNPEGKKYHVAAAFPSACGKTNFSMLVPPAGFDGWKVTTIGDDIAWIKPAADGKLYAINPEA
GYFGVAPGTNMLTNPNCMRSLDRDTIFTNVGLTDDGDVWWEGMEQDAPGKALPAHLIDWQGNDWTPQIAKETGAKAAHPN
SRFTVAATNNPALDSAWDDPKGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAATGQAGVVRRDPFAML
PFTGYNMSDYFQHWLDMGQKLAGSGAKLPKIYTTNWFRKGADGKFVWPGYGENMRVLKWMIDRIEGSGQGNDHITGVSPS
YEDLNWTGLDFSAEQFDSVISMDKAAWLKELELHAELFKQLEHHLPKELDQTMSAIERRLAA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=628, Percent_Identity=47.6114649681529, Blast_Score=584, Evalue=1e-167,
Organism=Homo sapiens, GI66346721, Length=609, Percent_Identity=48.9326765188834, Blast_Score=575, Evalue=1e-164,
Organism=Homo sapiens, GI66346723, Length=380, Percent_Identity=51.8421052631579, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989645, Length=614, Percent_Identity=45.2768729641694, Blast_Score=539, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71989658, Length=614, Percent_Identity=45.2768729641694, Blast_Score=538, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17555492, Length=623, Percent_Identity=46.2279293739968, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989653, Length=600, Percent_Identity=45.6666666666667, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI32564411, Length=563, Percent_Identity=49.0230905861456, Blast_Score=527, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI32564407, Length=531, Percent_Identity=48.7758945386064, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71989649, Length=489, Percent_Identity=48.2617586912065, Blast_Score=473, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI32564409, Length=377, Percent_Identity=44.2970822281167, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17540956, Length=549, Percent_Identity=26.0473588342441, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24655085, Length=609, Percent_Identity=48.9326765188834, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24655082, Length=609, Percent_Identity=48.2758620689655, Blast_Score=573, Evalue=1e-163,
Organism=Drosophila melanogaster, GI161077226, Length=566, Percent_Identity=49.2932862190813, Blast_Score=547, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24655088, Length=513, Percent_Identity=50.0974658869396, Blast_Score=506, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68300; Mature: 68300

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPVMQGLELGTPGYVKNAKLIAWVADMVALCKPDRVYWCDGSDAEYQRLCQQLVDAGT
CCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCC
FKKLNETKRPNSFLALSDPSDVARVEDRTYICSARKEDAGPTNNWMAPAEMRATLQPLFD
HHHHHHCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GCMQGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMKIMTRMGKAVFDVLGVNGDFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCEE
PCVHTVGAPLAAGQKDVTWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHEEEEEHH
NMGRDEGWLAEHMLILGVTNPEGKKYHVAAAFPSACGKTNFSMLVPPAGFDGWKVTTIGD
HCCCCCCHHEEEEEEEEEECCCCCEEEEEEECCHHCCCCCCEEEECCCCCCCEEEEEECC
DIAWIKPAADGKLYAINPEAGYFGVAPGTNMLTNPNCMRSLDRDTIFTNVGLTDDGDVWW
CCEEEEECCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHH
EGMEQDAPGKALPAHLIDWQGNDWTPQIAKETGAKAAHPNSRFTVAATNNPALDSAWDDP
CCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCC
KGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAATGQAGVVRRDPFAML
CCCEEEEEEECCCCCCEEEEHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEECCCEEEE
PFTGYNMSDYFQHWLDMGQKLAGSGAKLPKIYTTNWFRKGADGKFVWPGYGENMRVLKWM
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCHHHHHHH
IDRIEGSGQGNDHITGVSPSYEDLNWTGLDFSAEQFDSVISMDKAAWLKELELHAELFKQ
HHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHHLPKELDQTMSAIERRLAA
HHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHPVMQGLELGTPGYVKNAKLIAWVADMVALCKPDRVYWCDGSDAEYQRLCQQLVDAGT
CCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCC
FKKLNETKRPNSFLALSDPSDVARVEDRTYICSARKEDAGPTNNWMAPAEMRATLQPLFD
HHHHHHCCCCCCEEEECCCCHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GCMQGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMKIMTRMGKAVFDVLGVNGDFV
HHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCEE
PCVHTVGAPLAAGQKDVTWPCNKTKYIVHYPETREIWSYGSGYGGNALLGKKCFALRIAS
HHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHEEEEEHH
NMGRDEGWLAEHMLILGVTNPEGKKYHVAAAFPSACGKTNFSMLVPPAGFDGWKVTTIGD
HCCCCCCHHEEEEEEEEEECCCCCEEEEEEECCHHCCCCCCEEEECCCCCCCEEEEEECC
DIAWIKPAADGKLYAINPEAGYFGVAPGTNMLTNPNCMRSLDRDTIFTNVGLTDDGDVWW
CCEEEEECCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCHHH
EGMEQDAPGKALPAHLIDWQGNDWTPQIAKETGAKAAHPNSRFTVAATNNPALDSAWDDP
CCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCC
KGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAATGQAGVVRRDPFAML
CCCEEEEEEECCCCCCEEEEHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEECCCEEEE
PFTGYNMSDYFQHWLDMGQKLAGSGAKLPKIYTTNWFRKGADGKFVWPGYGENMRVLKWM
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCHHHHHHH
IDRIEGSGQGNDHITGVSPSYEDLNWTGLDFSAEQFDSVISMDKAAWLKELELHAELFKQ
HHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEHHLPKELDQTMSAIERRLAA
HHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA