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The map label for this gene is gidB

Identifier: 91785987

GI number: 91785987

Start: 77980

End: 78660

Strand: Direct

Name: gidB

Synonym: Bpro_0074

Alternate gene names: 91785987

Gene position: 77980-78660 (Clockwise)

Preceding gene: 91785986

Following gene: 91785988

Centisome position: 1.5

GC content: 62.7

Gene sequence:

>681_bases
ATGCGTGGCGGTGAGCAGGATTTGCGCGCCGGCCTGAAGGCGCTGCAACTGGAATTGACCGATAGCCAGGTCGGCCAGTT
GCTGGACTACCAGGACCTGATCGGCAAATGGACCAAGGTGTACAACCTTACGGCGGTGCGCGACCCGGTGGAAATGATGA
CCCACCATCTGCTGGACAGCCTGGCGGCGGTGCCGCCGCTGCGGCGCCATTTGCGCAAAACCGGGCAGGAGCAGGGCGCC
TGCCTGCTGGACGTGGGCTCGGGCGCCGGCCTTCCGGGCGTGGTGATCGCCATTTGTTGCCCGGAGGTCGCGGTGACCTG
CGTGGACACGGTGGCCAAGAAGGCGGCTTTCATCAAGCAGGCTGCGCTGGCGTTGAAGCTGCCCAATCTCTCGGGTTTGC
ATGCGCGCGTGGAAACGATCACCACACTGTTTGATGTGATCTGTTCCCGCGCCTTTGCCTCGTTGGCCGATTTCACCCAA
TGGTCTGGCGATGCACTGGCGTCCCATGGCGTGTGGATGGCCATGAAGGGGAAGCAACCAGCGGAAGAATTGGCCTCCTT
ACCACCGACCGTGGAAGTGTTTCACGTGGAACAGTTGCAGGTGCCGGGGCTGGATGCCGAGCGCTGTATTGTCTGGATGC
GGTGCAAGTCAGCTGCGGCAGGACTGGACCAGCAGGGCTAA

Upstream 100 bases:

>100_bases
CGACAGTCTCGCTGCTGCTGGTGCATCTGAAGAAAAACCTGTGGAAAAACACGGTGCCCCTGAAGACAACGGACACCTCC
ACCGAAAAAGCCCAAGCCTG

Downstream 100 bases:

>100_bases
AAAGAGGGATTTTCTTCGCCGTGTCGTTGTGCGCGGCACGAAAGACAGGTTCATTCGCGTTGGCGTCGTGACCCCTGTCG
TAAACTCAAGCCCTCTACTT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRGGEQDLRAGLKALQLELTDSQVGQLLDYQDLIGKWTKVYNLTAVRDPVEMMTHHLLDSLAAVPPLRRHLRKTGQEQGA
CLLDVGSGAGLPGVVIAICCPEVAVTCVDTVAKKAAFIKQAALALKLPNLSGLHARVETITTLFDVICSRAFASLADFTQ
WSGDALASHGVWMAMKGKQPAEELASLPPTVEVFHVEQLQVPGLDAERCIVWMRCKSAAAGLDQQG

Sequences:

>Translated_226_residues
MRGGEQDLRAGLKALQLELTDSQVGQLLDYQDLIGKWTKVYNLTAVRDPVEMMTHHLLDSLAAVPPLRRHLRKTGQEQGA
CLLDVGSGAGLPGVVIAICCPEVAVTCVDTVAKKAAFIKQAALALKLPNLSGLHARVETITTLFDVICSRAFASLADFTQ
WSGDALASHGVWMAMKGKQPAEELASLPPTVEVFHVEQLQVPGLDAERCIVWMRCKSAAAGLDQQG
>Mature_226_residues
MRGGEQDLRAGLKALQLELTDSQVGQLLDYQDLIGKWTKVYNLTAVRDPVEMMTHHLLDSLAAVPPLRRHLRKTGQEQGA
CLLDVGSGAGLPGVVIAICCPEVAVTCVDTVAKKAAFIKQAALALKLPNLSGLHARVETITTLFDVICSRAFASLADFTQ
WSGDALASHGVWMAMKGKQPAEELASLPPTVEVFHVEQLQVPGLDAERCIVWMRCKSAAAGLDQQG

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=208, Percent_Identity=40.3846153846154, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_POLSJ (Q12HF1)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_546939.1
- ProteinModelPortal:   Q12HF1
- SMR:   Q12HF1
- STRING:   Q12HF1
- GeneID:   4011076
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0074
- NMPDR:   fig|296591.1.peg.4904
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   Q12HF1
- ProtClustDB:   PRK00107
- BioCyc:   PSP296591:BPRO_0074-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 24310; Mature: 24310

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGEQDLRAGLKALQLELTDSQVGQLLDYQDLIGKWTKVYNLTAVRDPVEMMTHHLLDS
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAAVPPLRRHLRKTGQEQGACLLDVGSGAGLPGVVIAICCPEVAVTCVDTVAKKAAFIKQ
HHCCCHHHHHHHHCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALALKLPNLSGLHARVETITTLFDVICSRAFASLADFTQWSGDALASHGVWMAMKGKQP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCC
AEELASLPPTVEVFHVEQLQVPGLDAERCIVWMRCKSAAAGLDQQG
HHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRGGEQDLRAGLKALQLELTDSQVGQLLDYQDLIGKWTKVYNLTAVRDPVEMMTHHLLDS
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAAVPPLRRHLRKTGQEQGACLLDVGSGAGLPGVVIAICCPEVAVTCVDTVAKKAAFIKQ
HHCCCHHHHHHHHCCHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALALKLPNLSGLHARVETITTLFDVICSRAFASLADFTQWSGDALASHGVWMAMKGKQP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCC
AEELASLPPTVEVFHVEQLQVPGLDAERCIVWMRCKSAAAGLDQQG
HHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA