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The map label for this gene is hisF [H]

Identifier: 91785375

GI number: 91785375

Start: 4433326

End: 4434099

Strand: Reverse

Name: hisF [H]

Synonym: Bxe_A0404

Alternate gene names: 91785375

Gene position: 4434099-4433326 (Counterclockwise)

Preceding gene: 91785376

Following gene: 91785374

Centisome position: 90.57

GC content: 65.76

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGTCTCGACGTCACGGCTGGCCGCGTGGTCAAGGGCGTCAACTTCGTCGAACT
GCGCGATGCGGGCGACCCGGTCGAAATCGCCCGCCGCTACGACGATCAGGGCGCGGACGAACTCACCTTCCTCGACATCA
CCGCCACCTCGGATCAGCGCGATCTGATCCTGCCGATCATCGAAGCGGTCGCCTCGCAGGTGTTCATTCCGCTGACGGTC
GGCGGCGGCGTGCGCGCCGTCGAAGACGTGCGGCGCCTGTTGAACGCGGGCGCGGACAAGATCAGCATGAATTCGTCGGC
GGTGGCGAATCCGCAGCTCGTGAAAGACGCGACGGACAAATACGGCTCGCAGTGCATCGTGGTCGCGATCGACGCGAAGC
GCGTCTCCGCAGAAGGCGAAGTGCCGCGCTGGGAAGTCTTCACGCACGGCGGACGCAAGGCTACCGGGCTGGACGCGGTC
GAATGGGCGCGCAAGATGGCCGAACTCGGCGCCGGCGAAATCCTGCTCACCAGCATGGACCGCGACGGCACGAAGAGCGG
CTTCGATCTCGCTCTCACGCGCGCCGTGTCCGATGCGGTGCCGATCCCGGTGATCGCCTCGGGCGGCGTGGGCAACCTGC
AGCATCTGGCCGACGGCATCAAGGAAGGCCACGCGGACGCGGTACTCGCCGCGAGCATTTTCCACTACGGCGAACACACC
GTGGGCGAGGCCAAGCGCTTCATGGCCGATCAGGGCATTTCGGTGAGGTTGTGA

Upstream 100 bases:

>100_bases
GTTGCGTGGAACTCACCGGCGGCGCGACTGACCGCGCCGGCATTGTGCCGCTCATCCAGGCGAGCGGCCTCATCAGCGGT
ATTGGCAGAATTGCAAGATC

Downstream 100 bases:

>100_bases
CGTGGTGAATCCCTCGGCAGTCAATTGGCTCGACAAGGTCAGGTGGGACGCGAACGGCCTCGTGCCGGTGATCGCGCAGG
AAGCGTCGACGAACGACGTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVKDATDKYGSQCIVVAIDAKRVSAEGEVPRWEVFTHGGRKATGLDAV
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRAVSDAVPIPVIASGGVGNLQHLADGIKEGHADAVLAASIFHYGEHT
VGEAKRFMADQGISVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVKDATDKYGSQCIVVAIDAKRVSAEGEVPRWEVFTHGGRKATGLDAV
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRAVSDAVPIPVIASGGVGNLQHLADGIKEGHADAVLAASIFHYGEHT
VGEAKRFMADQGISVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKISMNSSAVANPQLVKDATDKYGSQCIVVAIDAKRVSAEGEVPRWEVFTHGGRKATGLDAVE
WARKMAELGAGEILLTSMDRDGTKSGFDLALTRAVSDAVPIPVIASGGVGNLQHLADGIKEGHADAVLAASIFHYGEHTV
GEAKRFMADQGISVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=41.6988416988417, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=30.5785123966942, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=34.4936708860759, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27272; Mature: 27141

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVKDATDK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHH
YGSQCIVVAIDAKRVSAEGEVPRWEVFTHGGRKATGLDAVEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRAVSDAVPIPVIASGGVGNLQHLADGIKEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VGEAKRFMADQGISVRL
HHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISMNSSAVANPQLVKDATDK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHH
YGSQCIVVAIDAKRVSAEGEVPRWEVFTHGGRKATGLDAVEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRAVSDAVPIPVIASGGVGNLQHLADGIKEGHADAVLAASIFHYGEHT
CCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
VGEAKRFMADQGISVRL
HHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA